ELOC (NM_005648) Human Untagged Clone

CAT#: SC116611

TCEB1 (untagged)-Human transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C) (TCEB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELOC
• Synonyms: SIII; TCEB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005648 edited
  ATGGATGGAGAGGAGAAAACCTATGGTGGCTGTGAAGGACCTGATGCCATGTATGTCAAA
  TTGATATCATCTGATGGCCATGAATTTATTGTAAAAAGAGAACATGCATTAACATCAGGC
  ACGATAAAAGCCATGTTGAGTGGCCCAGGTCAGTTTGCTGAGAACGAAACCAATGAGGTC
  AATTTTAGAGAGATACCTTCACATGTGCTATCGAAAGTATGCATGTATTTTACGTACAAG
  GTTCGCTACACTAACAGCTCCACCGAGATTCCTGAATTCCCAATTGCACCTGAAATTGCA
  CTGGAACTGCTGATGGCTGCGAACTTCTTAGATTGTTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005648 unedited
  GTTCAGATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGCCCGGACAGG
  CAGCTGGGGGTGGGGGGGCGGCCCTGGGATAGGGGCTGTGGCAGTACGCGGGGACCCGGC
  TGCGGTGGCTGCGGGACTGACGAGAAACTACTAAAGTTCCTGGGGAAGCAAAGTAGAATT
  TCATAAGAACAAAATGGATGGAGAGGAGAAAACCTATGGTGGCTGTGAAGGACCTGATGC
  CATGTATGTCAAATTGATATCATCTGATGGCCATGAATTTATTGTAAAAAGAGAACATGC
  ATTAACATCAGGCACGATAAAAGCCATGTTGAGTGGCCCAGGTCAGTTTGCTGAGAACGA
  AACCAATGAGGTCAATTTTAGAGAGATACCTTCACATGTGCTATCGAAAGTATGCATGTA
  TTTTACGTACAAGGTTCGCTACACTAACAGCTCCACCGAGATTCCTGAATTCCCAATTGC
  ACCTGAAATTGCACTGGAACTGCTGATGGCTGCGAACTTCTTAGATTGTTAAATAAAATA
  AATTATAATAAACTGTTAACTCTTTTCAGTATTTAATACCTGTAGTTCAGTTAGTAACTT
  TTTCATATATAGCATGTTGCCTGTATGCAGTTGAACTATATAAAGTTCATTGCAAAGCAG
  ATTATCTTGTTTTTTTGCATAGCAATCAAAGTTGAAATTTGTTTGCTACATCAACAAATT
  AAGGACCATTTCACAAACTGAGAAATAAACCAATATGCCAATTAAAAAAAAAAAAAAAAA
  CTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGGACAGATCCCGTTGGCATCC
  CTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGNNAGTTGCCACTCCAGTGCCACCAGC
  CTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005648 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTGGCATATTTGTTTA
  TTTCTCAGTTTGTGAAAATGTCCTTAATTTGTTGATGTAGCAAACAAATTTCAACTTTGA
  TTGCTATGCAAAAAAACAAGATAATCTGCTTTGCAATGAACTTTATATAGTTCAACTGCA
  TACAGGCAACATGCTATATATGAAAAAGTTACTAACTGAACTACAGGTATTAAATACTGA
  AAAGAGTTAACAGTTTATTATAATTTATTTTATTTAACAATCTAAGAAGTTCGCAGCCAT
  CAGCAGTTCCAGTGCAATTTCAGGTGCAATTGGGAATTCAGGAATCTCGGTGGAGCTGTT
  AGTGTAGCGAACCTTGTACGTAAAATACATGCATACTTTCGATAGCACATGTGAAGGTAT
  CTCTCTAAAATTGACCTCATTGGTTTCGTTCTCAGCAAACTGACCTGGGCCACTCAACAT
  GGCTTTTATCGTGCCTGATGTTAATGCATGTTCTCTTTTTACAATAAATTCATGGCCATC
  AGATGATATCAATTTGACATACATGGCATCAGGTCCTTCACAGCCACCATAGGTTNTCTC
  CTCTCCATCCATTTTGTTCTTATGAAATTCTACTTTGCTTCCCCAGGAACTTTAGTAGTT
  TCTCGTCAGTCCCGCAGCCACCGCAACCGGGTCCCCGCGTACTGCCACAGNCCCTATCCC
  ATGGCCGCCCCCCCACCCCCAATTGTCTGCTCCCGCCTCGTGCCGAATTTCGGGCCGCCC
  TATATTGAGTCGAATACAAAATCCTGACGGNTCACTAAACGACCTCTGCTTAATAAACCT
  CCACCGTACACGCTACCGCCCTTTGGGTAACGGGGCGGGGTATTACAACATTTGGAAGTC
  CGGTGGTTTGGCGCCAAAAAAACTCCCTTGCCGCAATGGGGTGGGAACCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005648
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005648.2, NP_005639.1
• RefSeq Size: 668 bp
• RefSeq ORF: 339 bp
• Locus ID: 6921
• Cytogenetics: 8q21.11
• Domains: Skp1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1-7 encode the same protein (isoform a).