TMPRSS2 (NM_005656) Human Untagged Clone

CAT#: SC116619

TMPRSS2 (untagged)-Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMPRSS2
• Synonyms: PP9284; PRSS10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116619 sequence for NM_005656 edited (data generated by NextGen Sequencing)
  ATGGCTTTGAACTCAGGGTCACCACCAGCTATTGGACCTTACTATGAAAACCATGGATAC
  CAACCGGAAAACCCCTATCCCGCACAGCCCACTGTGGTCCCCACTGTCTACGAGGTGCAT
  CCGGCTCAGTACTACCCGTCCCCCGTGCCCCAGTACGCCCCGAGGGTCCTGACGCAGGCT
  TCCAACCCCGTCGTCTGCACGCAGCCCAAATCCCCATCCGGGACAGTGTGCACCTCAAAG
  ACTAAGAAAGCACTGTGCATCACCTTGACCCTGGGGACCTTCCTCGTGGGAGCTGCGCTG
  GCCGCTGGCCTACTCTGGAAGTTCATGGGCAGCAAGTGCTCCAACTCTGGGATAGAGTGC
  GACTCCTCAGGTACCTGCATCAACCCCTCTAACTGGTGTGATGGCGTGTCACACTGCCCC
  GGCGGGGAGGACGAGAATCGGTGTGTTCGCCTCTACGGACCAAACTTCATCCTTCAGRTG
  TACTCATCTCAGAGGAAGTCCTGGCACCCTGTGTGCCAAGACGACTGGAACGAGAACTAC
  GGGCGGGCGGCCTGCAGGGACATGGGCTATAAGAATAATTTTTACTCTAGCCAAGGAATA
  GTGGATGACAGCGGATCCACCAGCTTTATGAAACTGAACACAAGTGCCGGCAATGTCGAT
  ATCTATAAAAAACTGTACCACAGTGATGCCTGTTCTTCAAAAGCAGTGGTTTCTTTACGC
  TGTATAGCCTGCGGGGTCAACTTGAACTCAAGCCGCCAGAGCAGGATTGTGGGCGGYGAG
  AGCGCGCTCCCGGGGGCCTGGCCCTGGCAGGTCAGCCTGCACGTCCAGAACGTCCACGTG
  TGCGGAGGCTCCATCATCACCCCCGAGTGGATCGTGACAGCCGCCCACTGCGTGGAAAAA
  CCTCTTAACAATCCATGGCATTGGACGGCATTTGCGGGGATTTTGAGACAATCTTTCATG
  TTCTATGGAGCCGGATACCAAGTAGAAAAAGTGATTTCTCATCCAAATTATGACTCCAAG
  ACCAAGAACAATGACATTGCGCTGATGAAGCTGCAGAAGCCTCTGACTTTCAACGACCTA
  GTGAAACCAGTGTGTCTGCCCAACCCAGGCATGATGCTGCAGCCAGAACAGCTCTGCTGG
  ATTTCCGGGTGGGGGGCCACCGAGGAGAAAGGGAAGACCTCAGAAGTGCTGAACGCTGCC
  AAGGTGCTTCTCATTGAGACACAGAGATGCAACAGCAGATATGTCTATGACAACCTGATC
  ACACCAGCCATGATCTGTGCCGGCTTCCTGCAGGGGAACGTCGATTCTTGCCAGGGTGAC
  AGTGGAGGGCCTCTGGTCACTTCGAAGAACAATATCTGGTGGCTGATAGGGGATACAAGC
  TGGGGTTCTGGCTGTGCCAAAGCTTACAGACCAGGAGTGTACGGGAATGTGATGGTATTC
  ACGGACTGGATTTATCGACAAATGAGGGCAGACGGCTAA
  
  Clone variation with respect to NM_005656.3
  478 g=>r;777 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005656 unedited
  NCCTGGATCTTGTGTGGGAGTCAGTGTCTACAAGCCTAACTTTTTTCGCGTTGGCTTGGT
  TGTTTGGAAAGTCATATTGAACATTCCAGATACCTATCATTACTCGATGCTGTTGATAAC
  AGCAAGATGGCTTTGAACTCAGGGTCACCACCAGCTATTGGATCGATTATGAAAACATGG
  ATACCATCCGGAAAACCTCTATCCCGCACAGCCCACTGTGGTCCCCACTGTCTACGAGGT
  GCATCCGGTTCAGTACTACCCGTCCCCCGTGCCCCAGTACGCCCCGAGGGTCCTGACGCA
  GGCTTCCAACCCCGTCGTCTGCACGCAGCCCAAATCCCCATCCGGGACAGTGTGCACCTC
  AAAGACTAAGAAAGCACTGTGCATCACCTTGACCCTGGGGACCTTCCTCGTGGGAGCTGC
  GCTGGCCGCTGGCCTACTCTGGAAGTTCATGGGCAGCAAGTGCTCCAACTCTGGGATAGA
  GTGCGACTCCTCAGGTACCTGCATCAACCCCTCTAACTGGTGTGATGGCGTGTCACACTG
  CCCCGGCGGGGAGGACGAGAATCGGTGTGTTCGCCTCTACGGACCAAACTTCATCCTTCA
  GGTGTACTCATCTCAGAGGAAGTCCTGGCACCCTGTGTGCCAAGACGACTGGGAACGAGA
  ACTACGGGCGGGCGGCCTGCAAGGACATGGGCTATAAGAATAATTTTTACTCTAGCCCAG
  GAATATTGGATGACAGCGGATCCACCAGCTTTATGAAACTGAACACAATTGTCCGGCAAT
  GTCGATATCTTATAAACACTGTCCCACAATGATGCCTGTTCTTCAAAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005656 unedited
  CTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTACTTTGAAA
  AAAAAACGCATAATTTATTTGCATGATATTCATTTTCACAATTGAACTTTACAGTTTAAA
  AAAGATACAAAAAAAGACAAACAGTTGTTCACATAAATAAGAAGGGGCAATAAAGAAGGA
  AGACGTTTTCACCATTACAACACCTTTTAGGATGTGTCTTGGGGAGCAAGCACCTTACAG
  TGCCAACTGTTTCCAAGGTCCCTGGGAATGCTGCTCTCTACAGAGGCATGTGCACAGACG
  GATCCTGCAAATGGGATTGCAAGACTTTCCATTTCAAGGTTAAGTCCTAGCTGTAGAATC
  ATTCATTTCATTCTTGCAAACCAGCCTGCTTGGCCAGGAGGCAGAACCATGGTAGAGTAG
  TGCTCATGGTTATGGCACTTGGCAATGCAAAAGGGACCCTTCCCCTGGTTGGAAACCCAC
  AGCATTGGAAGGGACCACAGAGAGTCCAAAACAAAACACATCTTTCTCTTCTTCGCCGCC
  ACCATGGGCACTTTGCTTCAGCACATTCATTTATAGAACGTTAAGTCAGGAGCCAGTCAT
  TTTGTCAAAGCAGCTGAAATAGGCCACCACTCTTACTTGGCAAAGATGATACAGTTTCAA
  AGAGTTAAATGAAGGTGGACTACTTGGAGACATCAAAAGCTAAGTTTCCAGGAGCACTGA
  GGTAGNCATGTGTAGAAACATAACATGGAAACAGTGTACCTTAAACTGAGCATCCTTGAT
  TTNCCCCATGGACCCCTCATTTCAGTGACTAGCAGGCCTGAAGAGGCAACATGGTGCCAG
  ACTTNGCGCCTGCCAGGACAGGNGCATGGCACTCTTNCAGGTGCTAGACAGGTCAGGNNA
  GATGAGAGAANGCTGATTCTCCTCCAGNAGCCCACCATGTGCAGTGGAAACTGCCCANGA
  TGCTAGGCAGTCCTTTCATGCTTCCTCGACAGGACTGT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005656
• Insert Size: 3270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005656.2, NP_005647.2
• RefSeq Size: 3226 bp
• RefSeq ORF: 1479 bp
• Locus ID: 7113
• Cytogenetics: 21q22.3
• Domains: SR, Tryp_SPc, ldl_recept_a
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2020]'
  Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1.