Legumain (LGMN) (NM_005606) Human Untagged Clone

CAT#: SC116627

LGMN (untagged)-Human legumain (LGMN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LGMN
• Synonyms: AEP; LGMN1; PRSC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116627 sequence for NM_005606 edited (data generated by NextGen Sequencing)
  ATGGTTTGGAAAGTAGCTGTATTCCTCAGTGTGGCCCTGGGCATTGGTGCCATTCCTATA
  GATGATCCTGAAGATGGAGGCAAGCACTGGGTGGTGATCGTGGCAGGTTCAAATGGCTGG
  TATAATTATAGGCACCAGGCAGACGCGTGCCATGCCTACCAGATCATTCACCGCAATGGG
  ATTCCTGACGAACAGATCGTTGTGATGATGTACGATGACATTGCTTACTCTGAAGACAAT
  CCCACTCCAGGAATTGTGATCAACAGGCCCAATGGCACAGATGTCTATCAGGGAGTCCCG
  AAGGACTACACTGGAGAGGATGTTACCCCACAAAATTTCCTTGCTGTGTTGAGAGGCGAT
  GCAGAAGCAGTGAAGGGCATAGGATCCGGCAAAGTCCTGAAGAGTGGCCCCCAGGATCAC
  GTGTTCATTTACTTCACTGACCATGGATCTACTGGAATACTGGTTTTTCCCAATGAAGAT
  CTTCATGTAAAGGACCTGAATGAGACCATCCATTACATGTACAAACACAAAATGTACCGA
  AAGATGGTGTTCTACATTGAAGCCTGTGAGTCTGGGTCCATGATGAACCACCTGCCGGAT
  AACATCAATGTTTATGCAACTACTGCTGCCAACCCCAGAGAGTCGTCCTACGCCTGTTAC
  TATGATGAGAAGAGGTCCACGTACCTGGGGGACTGGTACAGCGTCAACTGGATGGAAGAC
  TCGGACGTGGAAGATCTGACTAAAGAGACCCTGCACAAGCAGTACCACCTGGTAAAATCG
  CACACCAACACCAGCCACGTCATGCAGTATGGAAACAAAACAATCTCCACCATGAAAGTG
  ATGCAGTTTCAGGGTATGAAACGCAAAGCCAGTTCTCCCGTCCCCCTACCTCCAGTCACA
  CACCTTGACCTCACCCCCAGCCCTGATGTGCCTCTCACCATCATGAAAAGGAAACTGATG
  AACACCAATGATCTGGAGGAGTCCAGGCAGCTCACGGAGGAGATCCAGCGGCATCTGGAT
  GCCAGGCACCTCATTGAGAAGTCAGTGCGTAAGATCGTCTCCTTGCTGGCAGCGTCCGAG
  GCTGAGGTGGAGCAGCTCCTGTCCGAGAGAGCCCCGCTCACGGGGCACAGCTGCTACCCA
  GAGGCCCTGCTGCACTTCCGGACCCACTGCTTCAACTGGCACTCCCCCACGTACGAGTAT
  GCGTTGAGACATTTGTACGTGCTGGTCAACCTTTGTGAGAAGCCGTATCCACTTCACAGG
  ATAAAATTGTCCATGGACCACGTGTGCCTTGGTCACTACTGA
  
  Clone variation with respect to NM_005606.6
  52 g=>a;720 t=>c;1251 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005606 unedited
  ATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGGCCCGCAGCAATCACA
  GCAGTGCCGACGTCGTGGGTGTTTGGTGTGAGGCTGCGAGCCGCCGCGAGTTCTCACGGT
  CCCGCCGGCGCCACCACCGCGGTCACTCACCGCCGCCGCCGCCACCACTGCCACCACGGT
  CGCCTGCCACAGGTGTCTGCAATTGAACTCCAAGGTGCAGAATGGTTTGGAAAGTAGCTG
  TATTCCTCAGTGTGGCCCTGGGCATTGGTGCCATTCCTATAGATGATCCTGAAGATGGAG
  GCAAGCACTGGGTGGTGATCGTGGCAGGTTCAAATGGCTGGTATAATTATAGGCACCAGG
  CAGACGCGTGCCATGCCTACCAGATCATTCACCGCAATGGGATTCCTGACGAACAGATCG
  TTGTGATGATGTACGATGACATTGCTTACTCTGAAGACAATCCCACTCCAGGAATTGTGA
  TCAACAGGCCCAATGGCACAGATGTCTATCAGGGAGTCCCGAAGGACTACACTGGAGAGG
  ATGTTACCCCACAAAATTTCCTTGCTGTGTTGAGAGGCGATGCANAAGCAGTGAAGGGCA
  TAGGATCCGGCAAAGTCCTGAAGAGTGGCCCCCAGGATCACGTGTTCATTTACTTCACTG
  ACCATGGATCTACTGGAATACTGGTTTTTCCCAATGAAGATCTTCATGTAAAGGACCTGA
  ATGAGACCATNCATTACATGTACAAACACANAATGTACCGAAGGATGGTGTTCACATTGA
  AGCCTGTGAAGTCTGGGTCATGATGAACCACCTGCNCGATACATCATGGTTATGCACTAC
  TGCTGCCACCCAGAGAGTCGTCTACGCTGTACATGATNANAAAAAGTCCACTCCTGNGGG
  ACTGGTACGCGTCATGGATGGAAAACTCGACCGGGAGATTGCTAAGAGACCTGNCAGCAG
  TCCACTGTTAATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005606 unedited
  AGCTATGGACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGTTCTG
  TTATAAATCTTTATTTTTTAAGACTTGAACACCTGCAGAATTAAATACAAAAGCAATCAA
  AAATCATCATATTTTCTAAAAACAGTTTTCCATTTGGGGCTTGCGGCCCTCTTCAATAAA
  ATCATTCTGAAGATTTAAAGAGGTTTCAGCTTCAAATTCTCAGAATAAAGACTCCTTTTG
  AGAGGGGCTACAGAAGCCCCCATGAGCTTCCTGCTCCTCAAAACTAACAGGCAAAACAAC
  AAACCTCCTAGAAAGCAAATATGACCCTTCTCAATACAGAGCTTTTCCCACCCTAATTTG
  TAGTTTTTCAGAAAAGACTGGGGAAGCAGGTAACAGCGAGCAAGTCATCTTGTGAAGAAG
  GTCCTGGAGCGAGCCCTGGAGCGGGGGCTCCCCAGGAGGGCCCGAGCAGCGGAGACTTCT
  CACTCCACCTCTCCAGTCTCTGATCAGCACACAGTCGGTGGGGCGCTCACACTTGGAAAA
  GCTTCCAGGAGGCAGCTCTTCAGTAGTGACCAAGGCACACGTGGTCCATGGACAATTTTA
  TCCTGTGAAGTGGATACGGCTTCTCACAAAGGTTGACCAGCACGTACAAATGTCTCAACG
  CATACTCGTACGTGGGGGAGTGCCAGTTGAAGCAGTGGGTCCCGAAGTGCAGCAGGGCCT
  CTGGGTAGCAGCTGTGCCCCGTGNAGCGNGCTCTCTTCGACAGGAGCTGNTCCCCTNAGC
  CTCGNACGCTGCCACCAAGAGACGATCTTACGCACTGACTTTCTCATGAGTGCCTGCCAT
  CCAGATGCCGCTGGATCTCTCCCGGAGCTGCCTGGACTCCCCAGATCATTGNNGTCATCA
  GTTCCCTTTCATGATAGGGAGAGCACATCAAGCCTGGGGTGAGGCCCAGTGTGTGACTGG
  AG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005606
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005606.5, NP_005597.3
• RefSeq Size: 2048 bp
• RefSeq ORF: 1302 bp
• Locus ID: 5641
• Cytogenetics: 14q32.12
• Domains: Peptidase_C13
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome
• Gene Summary: 'This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same isoform.