PYGM (NM_005609) Human Untagged Clone

CAT#: SC116630

PYGM (untagged)-Human phosphorylase, glycogen, muscle (PYGM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYGM
• Synonyms: glycogen phosphorylase; glycogen storage disease type V; McArdle syndrome; muscle (McArdle syndrome, glycogen storage disease type V); myophosphorylase; phosphorylase, glycogen; phosphorylase, glycogen, muscle
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116630 sequence for NM_005609 edited (data generated by NextGen Sequencing)
  ATGTCCCGGCCCCTGTCAGACCAAGAGAAAAGAAAGCAAATCAGTGTGCGTGGCCTGGCC
  GGCGTGGAGAACGTGACTGAGCTGAAAAAGAACTTCAACCGGCACCTGCATTTCACACTC
  GTAAAGGACCGCAATGTGGCCACCCCACGAGACTACTACTTTGCTCTGGCCCATACCGTG
  CGCGACCACCTCGTGGGGCGCTGGATCCGCACGCAGCAGCACTACTATGAGAAGGACCCC
  AAGAGGATCTACTACCTGTCTTTAGAGTTCTATATGGGACGGACGCTACAGAACACCATG
  GTGAACCTGGCCTTAGAGAATGCCTGTGACGAGGCCACCTACCAGCTGGGCCTGGACATG
  GAGGAGCTGGAGGAAATTGAGGAGGATGCGGGGCTGGGCAACGGGGGCCTGGGCCGGCTG
  GCAGCCTGCTTTCTTGACTCCATGGCAACACTGGGCCTGGCCGCCTATGGCTACGGGATT
  CGCTATGAGTTTGGGATTTTTAACCAGAAGATCTCCGGGGGCTGGCAGATGGAGGAGGCC
  GATGACTGGCTTCGCTACGGCAACCCCTGGGAGAAGGCCCGGCCCGAGTTCACGCTACCT
  GTGCACTTCTACGGCCATGTGGAGCACACCAGCCAGGGTGCCAAGTGGGTGGACACACAG
  GTGGTACTGGCCATGCCCTACGATACGCCCGTGCCTGGCTATCGCAACAATGTTGTCAAC
  ACCATGCGCCTCTGGTCTGCCAAGGCTCCCAATGACTTCAACCTCAAGGACTTCAATGTC
  GGTGGCTACATCCAGGCTGTGTTGGACCGAAACCTGGCGGAGAACATCTCTCGTGTCCTG
  TACCCCAATGATAATTTCTTCGAAGGGAAGGAGCTGCGGCTGAAGCAGGAGTATTTCGTG
  GTGGCTGCCACCCTCCAGGACATCATCCGTCGCTTCAAGTCTTCCAAGTTCGGCTGCCGT
  GATCCCGTGCGCACGAACTTCGATGCCTTCCCAGATAAGGTGGCCATCCAGCTCAATGAC
  ACCCACCCCTCCCTGGCCATCCCCGAGCTGATGAGGATCCTGGTGGACCTGGAACGGATG
  GACTGGGACAAGGCGTGGGATGTGACAGTGAGGACCTGTGCCTACACCAACCACACGGTG
  CTGCCCGAGGCCCTGGAGCGCTGGCCGGTGCACCTCTTGGAGACGCTGCTGCCGCGGCAC
  CTCCAGATCATCTACGAGATCAACCAGCGCTTCCTCAACCGGGTGGCGGCCGCATTCCCA
  GGGGACGTAGACCGGCTGCGGCGCATGTCGCTGGTGGAGGAGGGCGCAGTGAAGCGCATC
  AACATGGCACACCTGTGCATCGCGGGGTCGCACGCCGTCAACGGCGTGGCGCGCATCCAC
  TCCGAGATCCTCAAGAAGACCATCTTCAAAGACTTCTATGAGCTGGAGCCTCATAAGTTC
  CAGAATAAGACCAACGGCATCACCCCTCGGCGCTGGCTGGTTCTGTGTAACCCCGGGCTG
  GCAGAGGTCATTGCTGAGCGCATCGGGGAGGACTTCATCTCTGACCTGGACCAGCTGCGC
  AAACTGCTCTCCTTTGTGGATGATGAAGCTTTCATTCGGGATGTGGCCAAAGTGAAGCAG
  GAAAACAAGTTGAAGTTTGCTGCCTACCTAGAGAGGGAATACAAAGTCCACATCAACCCC
  AACTCACTCTTCGACATCCAGGTGAAGCGGATTCACGAATATAAACGACAGCTCCTCAAC
  TGCCTCCATGTCATCACCCTGTACAACCGCATCAAGAGGGAGCCCAATAAGTTTTTTGTG
  CCTCGGACTGTGATGATTGGAGGGAAGGCTGCACCTGGGTACCACATGGCCAAGATGATC
  ATCAGACTCGTCACAGCCATCGGGGATGTGGTCAACCATGACCCGGCAGTGGGTGACCGC
  CTCCGTGTCATCTTCCTGGAGAACTACCGAGTCTCACTGGCCGAGAAAGTGATCCCAGCT
  GCAGACCTCTCTGAGCAGATCTCCACTGCGGGCACTGAAGCCTCAGGCACCGGCAACATG
  AAGTTCATGCTCAACGGGGCTCTGACCATTGGCACCATGGACGGGGCCAATGTGGAGATG
  GCAGAAGAGGCGGGAGAGGAAAACTTCTTCATCTTTGGCATGCGGGTGGAGGATGTGGAT
  AAGCTTGACCAAAGAGGGTACAATGCCCAGGAGTACTACGATCGCATTCCTGAGCTTCGG
  CAGGTCATTGAGCAGCTGAGCAGTGGCTTCTTCTCCCCCAAACAGCCCGACCTGTTCAAG
  GACATTGTCAATATGCTCATGCACCATGACCGGTTTAAAGTCTTCGCAGATTATGAAGAC
  TACATTAAATGCCAGGAGAAAGTCAGCGCCTTGTACAAGAACCCAAGAGAGTGGACGCGG
  ATGGTGATCCGGAACATAGCCACCTCTGGCAAGTTCTCCAGTGACCGCACCATTGCCCAG
  TATGCCCGGGAGATCTGGGGTGTGGAGCCTTCCCGCCAGCGCCTGCCAGCCCCGGATGAG
  GCCATCTGA
  
  Clone variation with respect to NM_005609.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005609 unedited
  GTTGGATTTTGTATACGACTTATATAGGCGGCCGCGCAATTCGCACGAGGCTGGAGGCAG
  TGCTGAGGCCGCCGTCCCCTCTACCATCAGTCCAGTCCGGCCCGCCCTCCTGCAGCCATG
  TCCCGGCCCCTGTCAGACCAAGAGAAAAGAAAGCAAATCAGTGTGCGTGGCCTGGCCGGC
  GTGGAGAACGTGACTGAGCTGAAAAAGAACTTCAACCGGCACCTGCATTTCACACTCGTA
  AAGGACCGCAATGTGGCCACCCCACGAGACTACTACTTTGCTCTGGCCCATACCGTGCGC
  GACCACCTCGTGGGGCGCTGGATCCGCACGCAGCAGCACTACTATGAGAAGGACCCCAAG
  AGGATCTACTACCTGTCTTTAGAGTTCTATATGGGACGGACGCTACAGAACACCATGGTG
  AACCTGGCCTTAGAGAATGCCTGTGACGAGGCCACCTACCAGCTGGGCCTGGACATGGAG
  GAGCTGGAGGAAATTGAGGAGGATGCGGGGCTGGGCAACGGGGGCCTGGGCCGGCTGGCA
  GCCTGCTTTCTTGACTCCATGGCAACACTGGGCCTGGCCGCCTATGGCTACGGGATTCGC
  TATGAGTTTGGGATTTTTAACCAGAAGATCTCCGGGGGCTGGCAGATGGAGGAGGCCGAT
  GACTGGCTTCGCTACGGCAACCCCTGGGAGAAGGCCCGGCCCGAGTTCACGCTACCTGTG
  CACTTCTACGGCCATGTGGAGCACACCAGCCAGGGTGCCAAGTGGGGTGACACACANGTG
  GTACTGGCCATGCCCTACGATACGCCCGTGCCTGGCTATCGCAACATGGTTGTCAACACA
  TGCGCCTCTGGTCTGCCAAGGCTCCNATGACTTNNCACCTCAGGACTTCAATGTCGTGGC
  TACATCCAGNCTGTGTTGGACCGAAACCTGGCGGAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005609 unedited
  NNNTTTACTCTGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
  CCCAGGGAGAATGAATTTATTAGGGAGTGGGTGCAGTTGGTCAGACCCCATAAATAGGAG
  GGGACCGGGAGCCCGAGGACGGAAGGGGGCCCGTGTCCTTAGTCACGCTGGACACTGGGA
  CATTGAGAGTGGGGAAAATGAGGGTTCCAGGAGGGGCTTAAAGATCTAACTCCAGTACCC
  CACCCTCTGCATGAGGTGCTGGGGCTGGCCCAAGAGAGTGTGACAGACTCAAGGGCTGGT
  TTGGGGTCTGGTCTGGAGGCTCAAATGGCCTCATCCGGGGCTGGCAGGCGCTGGCGGGAA
  GGCTCCACACCCCAGATCTCCCGGGCATACTGGGCAATGGTGCGGTCACTGGAGAACTTG
  CCAGAGGTGGCTATGTTCCGGATCACCATCCGCGTCCACTCTCTTGGGTTCTTGTACAAG
  GCGCTGACTTTCTCCTGGCATTTAATGTAGTCTTCATAATCTGCGAAGACTTTAAACCGG
  TCATGGTGCATGAGCATATTGACAATGTCCTTGAACAGGTCGGGCTGTTTGGGGGAGAAG
  AAGCCACTGCTCAGCTGCTCAATGACCTGCCGAAGCTCAGGAATGCGATCGTAGTACTCC
  TGGGCATTGTACCCTCTTTGGTCAAGCTTATCCACATCCTCCACCCGCATGCCAAAGATG
  AAGAAGTTTTCCTCTCCCGCCTCTTCTGCCATCTCCACATTGGGCCCCGTNCATGGTGCC
  ANATGGTCANAGCCCCCGTTGAGCATGAACTTNCATGTNGCCGTGCCTGAAGCTTCANTG
  CCCGCAGTGGAGATCTGCTCANAAAGTCTGGAGCTGGGATCACTTTCTGGCCAGTGAGAT
  CGGTAGTTCTCCAGGAAGATGACCCGAGCGGGCACCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005609
• Insert Size: 2780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005609.1, NP_005600.1
• RefSeq Size: 3447 bp
• RefSeq ORF: 2529 bp
• Locus ID: 5837
• Cytogenetics: 11q13.1
• Domains: phosphorylase
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Gene Summary: 'This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).