RbAp48 (RBBP4) (NM_005610) Human Untagged Clone

CAT#: SC116632

RBBP4 (untagged)-Human retinoblastoma binding protein 4 (RBBP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBBP4
• Synonyms: lin-53; NURF55; RBAP48
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005610, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGACAAGGAAGCAGCCTTCGACGACGCAGTGGAAGAACGAGTGATCAACGAGGAATACAAAATAT
  GGAAAAAGAACACCCCTTTTCTTTATGATTTGGTGATGACCCATGCTCTGGAGTGGCCCAGCCTAACTGC
  CCAGTGGCTTCCAGATGTAACCAGACCAGAAGGGAAAGATTTCAGCATTCATCGACTTGTCCTGGGGACA
  CACACATCGGATGAACAAAACCATCTTGTTATAGCCAGTGTGCAGCTCCCTAATGATGATGCTCAGTTTG
  ATGCGTCACACTACGACAGTGAGAAAGGAGAATTTGGAGGTTTTGGTTCAGTTAGTGGAAAAATTGAAAT
  AGAAATCAAGATCAACCATGAAGGAGAAGTAAACAGGGCCCGTTATATGCCCCAGAACCCTTGTATCATC
  GCAACAAAGACTCCTTCCAGTGATGTTCTTGTTTTTGACTATACAAAACATCCTTCTAAACCAGATCCTT
  CTGGAGAGTGCAACCCAGACTTGCGTCTCCGTGGACATCAGAAGGAAGGCTATGGGCTTTCTTGGAACCC
  AAATCTCAGTGGGCACTTACTTAGTGCTTCAGATGACCATACCATCTGCCTGTGGGACATCAGTGCCGTT
  CCAAAGGAGGGAAAAGTGGTAGATGCGAAGACCATCTTTACAGGGCATACGGCAGTAGTAGAAGATGTTT
  CCTGGCATCTACTCCATGAGTCTCTGTTTGGGTCAGTTGCTGATGATCAGAAACTTATGATTTGGGATAC
  TCGTTCAAACAATACTTCCAAACCAAGCCACTCAGTTGATGCTCACACTGCTGAAGTGAACTGCCTTTCT
  TTCAATCCTTATAGTGAGTTCATTCTTGCCACAGGATCAGCTGACAAGACTGTTGCCTTGTGGGATCTGA
  GAAATCTGAAACTTAAGTTGCATTCCTTTGAGTCACATAAGGATGAAATATTCCAGGTTCAGTGGTCACC
  TCACAATGAGACTATTTTAGCTTCCAGTGGTACTGATCGCAGACTGAATGTCTGGGATTTAAGTAAAATT
  GGAGAGGAACAATCCCCAGAAGATGCAGAAGACGGGCCACCAGAGTTGTTGTTTATTCATGGTGGTCATA
  CTGCCAAGATATCTGATTTCTCCTGGAATCCCAATGAACCTTGGGTGATTTGTTCTGTATCAGAAGACAA
  TATCATGCAAGTGTGGCAAATGGCAGAGAACATTTATAATGATGAAGACCCTGAAGGAAGCGTGGATCCA
  GAAGGACAAGGGTCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005610 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAGAGCGAGCTCT
  TGCAGCCTCCCCGCCCCTCCCGCAACGCTCGACCCCAGGATTCCCCCGGCTCGCCTGCCC
  GCCATGGCCGACAAGGAAGCAGCCTTCGACGACGCAGTGGAAGAACGAGTGATCAACGAG
  GAATACAAAATATGGAAAAAGAACACCCCTTTTCTTTATGATTTGGTGATGACCCATGCT
  CTGGAGTGGCCCAGCCTAACTGCCCAGTGGCTTCCAGATGTAACCAGACCAGAAGGGAAA
  GATTTCAGCATTCATCGACTTGTCCTGGGGACACACACATCGGATGAACAAAACCATCTT
  GTTATAGCCAGTGTGCAGCTCCCTAATGATGATGCTCAGTTTGATGCGTCACACTACGAC
  AGTGAGAAAGGAGAATTTGGAGGTTTTGGTTCAGTTAGTGGAAAAATTGAAATAGAAATC
  AAGATCAACCATGAAGGAGAAGTAAACAGGGCCCGTTATATGCCCCAGAACCCTTGTATC
  ATCGCAACAAAGACTCCTTCCAGTGATGTTCTTGTCTTTGACTATACAAAACATCCTTCT
  AAACCAGATCCTTCTGGAGAGTGCAACCCAGACTTGCGTCTCCGTGGACATCAGAAGGAA
  GGCTATGGGCTTTCTTGGAACCCANATCTCAGTGGGCACTTACTTAGTGCTTCAGATGAC
  CATACCATCTGCCTGTGGGACATCAGTGCCCTTCCCAAAGAAGGAAAAGTGGTAGATGCG
  AAGACCCATCTTACAGGGCATACGGCAGTAGTAGAAGATGTTCCTGGCATCTACTCCATG
  AGTCTCTGTTTGGGCCAGTGCTGATGATCAAAAACTATGATTTGGGATACTCGCTCAAAC
  AT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005610 unedited
  TATGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTATGTAGAAA
  AACAATTTATTCCATTTTAAGCACTTACACAGTTAGTCATGGAGAGTAACAGGCCTGCTG
  GTGAAACAGGTCACCCAAAATGGAGATGGCATCAAACTAGTGGTCAAGGACTAACTCCTA
  AAAAAAAAAACAACTCTTATCAAGGATTAATTTAATTTTTAAAACAAATACAAGTTATTG
  ATTCACTCTTCTCAACTTGACAGTCTACCTGTGGTATAACTGTCAGGTAAAAACATACAT
  CTTTACAACTTGGTGGTCCCAAGTTAAAAAAAAAAAAAAAAAAACCAACAACAAAAAAAA
  AAACACCATTTCACAGACAGGAAATAAACAACATGAAAACAGCTCAAGAAATACACTAAC
  GAGCAAAAATATATGAATATATGGGGAAAGAGGAACGTGTTGTTTTGACTTAACTGAAAA
  AACCAAGAGGAAACTGGTCTACGTATGAAAATGTGCATCCTGGAAAGTCAGGTGTCAAGA
  TTTTCGAGTAGGAATCTATACGACTTGAATCTCCCCCTATTTCCTGAATAAAAGTGACAT
  CTTTCAGTATTTATACTTCATGGCTCAAACACCTACCTCATTTGGCTCTATTCCTTACTC
  ACTCTAGCCTTTACTTAAATGAGTCTGAAAAACTTGGGGATATAGCATAAGAAGAAAAAT
  AATCACACATAATATTCCCCTTTCTGTAGCTACTTTAGACCTGNGTTACTAGAAAATTCC
  TGAAGAAAATTTCAACGTAAGTCTGTGGCTTNTGTGAATCAAGCCCCCCTATAAATTTAG
  AAAACACCCACGTTTGGCTATAGCATTATCGTGGTACTATTTATANAGGGAAGCTGATAA
  AGN
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005610
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005610.1, NP_005601.1
• RefSeq Size: 2314 bp
• RefSeq ORF: 1278 bp
• Locus ID: 5928
• Cytogenetics: 1p35.1
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.