Stathmin 1 (STMN1) (NM_005563) Human Untagged Clone

CAT#: SC116654

STMN1 (untagged)-Human stathmin 1 (STMN1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STMN1
• Synonyms: C1orf215; Lag; LAP18; OP18; PP17; PP19; PR22; SMN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005563 edited
  ATGGCTTCTTCTGATATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCCAGGCTTTT
  GAGCTGATTCTCAGCCCTCGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCCCCTCCA
  AAGAAGAAGGATCTTTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGA
  CGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAA
  GAAGTGCTTCAGAAGGCAATAGAAGAGAACAACAACTTCAGTAAAATGGCAGAAGAGAAA
  CTGACCCACAAAATGGAAGCTAATAAAGAGAACCGAGAGGCACAAATGGCTGCCAAACTG
  GAACGTTTGCGAGAGAAGGATAAGCACATTGAAGAAGTGCGGAAGAACAAAGAATCCAAA
  GACCCTGCTGACGAGACTGAAGCTGACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005563 unedited
  NGGTCGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCTCGGACTG
  AGCAGGACTTTCCTTATCCCAGTTGATTGTGCAGAAACACTGCCTGTCGCTTGTCTTCTA
  TTCACCATGGCTTCTTCTGATATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCCAG
  GCTTTTGAGCTGATTCTCAGCCCTCGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCC
  CCTCCAAAGAAGAAGGATCTTTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAA
  GAAAGACGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCAC
  GAGAAAGAAGTGCTTCAGAAGGCAATAGAAGAGAACAACAACTTCAGTAAAATGGCAGAA
  GAGAAACTGACCCACAAAATGGAAGCTAATAAAGAGAACCGAGAGGCACAAATGGCTGCC
  AAACTGGAACGTTTGCGAGAGAAGGATAAGCACATTGAAGAAGTGCGGAAGAACAAAGAA
  TCCAAAGACCCTGCTGACGAGACTGAAGCTGACTAATTTGTTCTGAGAACTGACTTTCTC
  CCCATCCCCTTCCTAAATATCCAAAGACTGTACTGGCCAGTGTCATTNTATTTTTTCCCT
  CCTGNACAATATTTTAGAAGCTAATGTANGACTGTATAGGTAGATCCAGATCCAGACTGG
  TAGATGTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTNTACTCTTTTTCTAANA
  GTGTGGTCTTTCTAATGTAGCTATTNTTCCTTGGTGCATCTTTTCTACTTCAGTACCTTT
  GGTGTACTGGGGTTATGGGCTAGTACTGGNATTGGCTCTGTGAAACATATTTGTGAAAAG
  ATATGTAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005563
• Insert Size: 1030 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005563.3, NP_005554.1
• RefSeq Size: 1542 bp
• RefSeq ORF: 450 bp
• Locus ID: 3925
• Cytogenetics: 1p36.11
• Domains: Stathmin
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]'
  Transcript Variant: This variant (3) uses an alternate terminal exon, compared to variant 4. The resulting isoform (a) has a shorter and distinct C-terminus, compared to isoform b. Variants 1, 2, and 3 encode the same protein.