LDHA (NM_005566) Human Untagged Clone

CAT#: SC116656

LDHA (untagged)-Human lactate dehydrogenase A (LDHA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LDHA
• Synonyms: GSD11; HEL-S-133P; LDHM; PIG19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116656 sequence for NM_005566 edited (data generated by NextGen Sequencing)
  ATGGCAACTCTAAAGGATCAGCTGATTTATAATCTTCTAAAGGAAGAACAGACCCCCCAG
  AATAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCCTGTGCCATCAGTATCTTA
  ATGAAGGACTTGGCAGATGAACTTGCTCTTGTTGATGTCATCGAAGACAAATTGAAGGGA
  GAGATGATGGATCTCCAACATGGCAGCCTTTTCCTTAGAACACCAAAGATTGTCTCTGGC
  AAAGACTATAATGTAACTGCAAACTCCAAGCTGGTCATTATCACGGCTGGGGCACGTCAG
  CAAGAGGGAGAAAGCCGTCTTAATTTGGTCCAGCGTAACGTGAACATCTTTAAATTCATC
  ATTCCTAATGTTGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCCAGTG
  GATATCTTGACCTACGTGGCTTGGAAGATAAGTGGTTTTCCCAAAAACCGTGTTATTGGA
  AGTGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTGATGGGGGAAAGGCTGGGAGTT
  CACCCATTAAGCTGTCATGGGTGGGTCCTTGGGGAACATGGAGATTCCAGTGTGCCTGTA
  TGGAGTGGAATGAATGTTGCTGGTGTCTCTCTGAAGACTCTGCACCCAGATTTAGGGACT
  GATAAAGATAAGGAACAGTGGAAAGAGGTTCACAAGCAGGTGGTTGAGAGTGCTTATGAG
  GTGATCAAACTCAAAGGCTACACATCCTGGGCTATTGGACTCTCTGTAGCAGATTTGGCA
  GAGAGTATAATGAAGAATCTTAGGCGGGTGCACCCAGTTTCCACCATGATTAAGGGTCTT
  TACGGAATAAAGGATGATGTCTTCCTTAGTGTTCCTTGCATTTTGGGACAGAATGGAATC
  TCAGACCTTGTGAAGGTGACTCTGACTTCTGAGGAAGAGGCCCGTTTGAAGAAGAGTGCA
  GATACACTTTGGGGGATCCAAAAGGAGCTGCAATTTTAA
  
  Clone variation with respect to NM_005566.3
  483 c=>t;519 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005566 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGCCGCCGATTCCGGATCTCA
  TTGCCACGCGCCCCCGACGACCGCCCGACGTGCATTCCCGATTCCTTTTGGTTCCAAGTC
  CAATATGGCAACTCTAAAGGATCAGCTGATTTATAATCTTCTAAAGGAAGAACAGACCCC
  CCAGAATAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCCTGTGCCATCAGTAT
  CTTAATGAAGGACTTGGCAGATGAACTTGCTCTTGTTGATGTCATCGAAGACAAATTGAA
  GGGAGAGATGATGGATCTCCAACATGGCAGCCTTTTCCTTAGAACACCAAAGATTGTCTC
  TGGCAAAGACTATAATGTAACTGCAAACTCCAAGCTGGTCATTATCACGGCTGGGGCACG
  TCAGCAAGAGGGAGAAAGCCGTCTTAATTTGGTCCAGCGTAACGTGAACATCTTTAAATT
  CATCATTCCTAATGTTGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCC
  AGTGGATATCTTGACCTACGTGGCTTGGAAGATAAGTGGTTTTCCCAAAAACCGTGTTAT
  TGGAAAGTGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTGATGGGGGAAAGGGCTG
  GGAGTTCACCCATTAAGCTGTCATGGGGTGGGTCCTTGGGGAACATGGAGATTCCAGTGT
  GCCTGTATGGAGTGGAATGATGNTGCCTGGTGTCTCTCTGAGACTCTGCCCCAGATTANG
  GACTGATAAGATAAGGACAGTGNAAAAGAGTCACAAGCAGGTGGTTGAAAGTCTTATGGA
  GTGATCAACTCAAAGCTACCATNCTGGGCTATGGACTCTTGTAGCGATTTGCAGAAATTT
  ATGGAGATCTTAGGCGGTGCCCCAGTTCACATGAATAAAGGTCTTACGAAAGAGGAGAAG
  CTCCCCTAGGTCCTGCATTGGGACAATGAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005566 unedited
  GCTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTAACTGTTAAAGGTTTATGGGGGGTTTTAGTTGGTATAACACTTGGATAGT
  GGGTTGCATTGTTTGTATGTAAATCTTTTTACATTATATGGAAAGGTCCACTACTGATAT
  AGTTCACAAAATAAAATCCTTTGGAAAAAATTATGCACAAAACATGATATTGGATTTATA
  CACTGGATCCCAGGATGGGACTCACTGGGAAAAAAATGTTGGACTAGGCATGTTCAGGGA
  AGGACCCAGGAAGTTATATAACACACGGTAAACATCCACCTGGCTCAAGGGGCAAATGCA
  GTACGTACAGCATTGGCAGGGGGGCGTCAAAGGGGGCAAAACTATTTCACACTACCCAGT
  TGAAGACTACCCAAGATTAATCCCTTCCAGCATCAGGATATAGCTGGGGATTTTACAAAC
  CATTCTTATTTCTAACTTCAGGAGTTGATGTTTTTCCCAGCCCATCTTAAAATATTACTG
  CTTTATTCACAGATCAGATAAAAAGGACAACTTGCCCAACCTCCACCTAAAATCCTGTTG
  TACCCTAAACAGGGAAATGATATGACATCAAAAGACTTTAAAATTGCAGCTCCTTTTGGA
  TCCCCCAAAGTGTATCTGCACTCTTCTTAAAACGGGCCTCTTCCTCAAAAGTCAGAGTCA
  CCTTCACAAGGTCTGAGATTCCATTCTGTCCCAAATGCAAGGACACTAAGGAAAACATCT
  CCTTTATTCCGTAAAACCCTTATCATGGTGGAAACTGGGTGCACCCGCCTAAGATTCTTA
  TTATACTCTCTGCCAAATCTCTACAGAGAGTCCATAGCCCAGAATGTGTAGCCTTGGAGT
  TGACACCTATAGCACTCTAACCACTGCTGGGACCCTTTTCATTGTTCTTACTTATAGCCC
  TAAATTGGNNGCAATCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005566
• Insert Size: 1690 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005566.1, NP_005557.1
• RefSeq Size: 1661 bp
• RefSeq ORF: 999 bp
• Locus ID: 3939
• Cytogenetics: 11p15.1
• Domains: ldh
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
• Gene Summary: 'The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.