LDHA (NM_005566) Human Untagged Clone

CAT#: SC116656

LDHA (untagged)-Human lactate dehydrogenase A (LDHA), transcript variant 1


  "NM_005566" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "LDHA"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LDHA
Synonyms GSD11; HEL-S-133P; LDHM; PIG19
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116656 sequence for NM_005566 edited (data generated by NextGen Sequencing)
ATGGCAACTCTAAAGGATCAGCTGATTTATAATCTTCTAAAGGAAGAACAGACCCCCCAG
AATAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCCTGTGCCATCAGTATCTTA
ATGAAGGACTTGGCAGATGAACTTGCTCTTGTTGATGTCATCGAAGACAAATTGAAGGGA
GAGATGATGGATCTCCAACATGGCAGCCTTTTCCTTAGAACACCAAAGATTGTCTCTGGC
AAAGACTATAATGTAACTGCAAACTCCAAGCTGGTCATTATCACGGCTGGGGCACGTCAG
CAAGAGGGAGAAAGCCGTCTTAATTTGGTCCAGCGTAACGTGAACATCTTTAAATTCATC
ATTCCTAATGTTGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCCAGTG
GATATCTTGACCTACGTGGCTTGGAAGATAAGTGGTTTTCCCAAAAACCGTGTTATTGGA
AGTGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTGATGGGGGAAAGGCTGGGAGTT
CACCCATTAAGCTGTCATGGGTGGGTCCTTGGGGAACATGGAGATTCCAGTGTGCCTGTA
TGGAGTGGAATGAATGTTGCTGGTGTCTCTCTGAAGACTCTGCACCCAGATTTAGGGACT
GATAAAGATAAGGAACAGTGGAAAGAGGTTCACAAGCAGGTGGTTGAGAGTGCTTATGAG
GTGATCAAACTCAAAGGCTACACATCCTGGGCTATTGGACTCTCTGTAGCAGATTTGGCA
GAGAGTATAATGAAGAATCTTAGGCGGGTGCACCCAGTTTCCACCATGATTAAGGGTCTT
TACGGAATAAAGGATGATGTCTTCCTTAGTGTTCCTTGCATTTTGGGACAGAATGGAATC
TCAGACCTTGTGAAGGTGACTCTGACTTCTGAGGAAGAGGCCCGTTTGAAGAAGAGTGCA
GATACACTTTGGGGGATCCAAAAGGAGCTGCAATTTTAA

Clone variation with respect to NM_005566.3
483 c=>t;519 a=>g
>OriGene 5' read for NM_005566 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGCCGCCGATTCCGGATCTCA
TTGCCACGCGCCCCCGACGACCGCCCGACGTGCATTCCCGATTCCTTTTGGTTCCAAGTC
CAATATGGCAACTCTAAAGGATCAGCTGATTTATAATCTTCTAAAGGAAGAACAGACCCC
CCAGAATAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCCTGTGCCATCAGTAT
CTTAATGAAGGACTTGGCAGATGAACTTGCTCTTGTTGATGTCATCGAAGACAAATTGAA
GGGAGAGATGATGGATCTCCAACATGGCAGCCTTTTCCTTAGAACACCAAAGATTGTCTC
TGGCAAAGACTATAATGTAACTGCAAACTCCAAGCTGGTCATTATCACGGCTGGGGCACG
TCAGCAAGAGGGAGAAAGCCGTCTTAATTTGGTCCAGCGTAACGTGAACATCTTTAAATT
CATCATTCCTAATGTTGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCC
AGTGGATATCTTGACCTACGTGGCTTGGAAGATAAGTGGTTTTCCCAAAAACCGTGTTAT
TGGAAAGTGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTGATGGGGGAAAGGGCTG
GGAGTTCACCCATTAAGCTGTCATGGGGTGGGTCCTTGGGGAACATGGAGATTCCAGTGT
GCCTGTATGGAGTGGAATGATGNTGCCTGGTGTCTCTCTGAGACTCTGCCCCAGATTANG
GACTGATAAGATAAGGACAGTGNAAAAGAGTCACAAGCAGGTGGTTGAAAGTCTTATGGA
GTGATCAACTCAAAGCTACCATNCTGGGCTATGGACTCTTGTAGCGATTTGCAGAAATTT
ATGGAGATCTTAGGCGGTGCCCCAGTTCACATGAATAAAGGTCTTACGAAAGAGGAGAAG
CTCCCCTAGGTCCTGCATTGGGACAATGAAT
>OriGene 3' read for NM_005566 unedited
GCTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTAACTGTTAAAGGTTTATGGGGGGTTTTAGTTGGTATAACACTTGGATAGT
GGGTTGCATTGTTTGTATGTAAATCTTTTTACATTATATGGAAAGGTCCACTACTGATAT
AGTTCACAAAATAAAATCCTTTGGAAAAAATTATGCACAAAACATGATATTGGATTTATA
CACTGGATCCCAGGATGGGACTCACTGGGAAAAAAATGTTGGACTAGGCATGTTCAGGGA
AGGACCCAGGAAGTTATATAACACACGGTAAACATCCACCTGGCTCAAGGGGCAAATGCA
GTACGTACAGCATTGGCAGGGGGGCGTCAAAGGGGGCAAAACTATTTCACACTACCCAGT
TGAAGACTACCCAAGATTAATCCCTTCCAGCATCAGGATATAGCTGGGGATTTTACAAAC
CATTCTTATTTCTAACTTCAGGAGTTGATGTTTTTCCCAGCCCATCTTAAAATATTACTG
CTTTATTCACAGATCAGATAAAAAGGACAACTTGCCCAACCTCCACCTAAAATCCTGTTG
TACCCTAAACAGGGAAATGATATGACATCAAAAGACTTTAAAATTGCAGCTCCTTTTGGA
TCCCCCAAAGTGTATCTGCACTCTTCTTAAAACGGGCCTCTTCCTCAAAAGTCAGAGTCA
CCTTCACAAGGTCTGAGATTCCATTCTGTCCCAAATGCAAGGACACTAAGGAAAACATCT
CCTTTATTCCGTAAAACCCTTATCATGGTGGAAACTGGGTGCACCCGCCTAAGATTCTTA
TTATACTCTCTGCCAAATCTCTACAGAGAGTCCATAGCCCAGAATGTGTAGCCTTGGAGT
TGACACCTATAGCACTCTAACCACTGCTGGGACCCTTTTCATTGTTCTTACTTATAGCCC
TAAATTGGNNGCAATCTN
Restriction Sites NotI-NotI     
ACCN NM_005566
Insert Size 1690 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005566.1, NP_005557.1
RefSeq Size 1661 bp
RefSeq ORF 999 bp
Locus ID 3939
Cytogenetics 11p15.1
Domains ldh
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
Gene Summary 'The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]'
Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.