Lamin B1 (LMNB1) (NM_005573) Human Untagged Clone

CAT#: SC116661

LMNB1 (untagged)-Human lamin B1 (LMNB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LMNB1
• Synonyms: ADLD; LMN; LMN2; LMNB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116661 sequence for NM_005573 edited (data generated by NextGen Sequencing)
  ATGGCGACTGCGACCCCCGTGCCGCCGCGGATGGGCAGCCGCGCTGGCGGCCCCACCACG
  CCGCTGAGCCCCACGCGCCTGTCGCGGCTCCAGGAGAAGGAGGAGCTGCGCGAGCTCAAT
  GACCGGCTGGCGGTGTACATCGACAAGGTGCGCAGCCTGGAGACGGAGAACAGCGCGCTG
  CAGCTGCAGGTGACGGAGCGCGAGGAGGTGCGCGGCCGTGAGCTCACCGGCCTCAAGGCG
  CTCTACGAGACCGAGCTGGCCGACGCGCGACGCGCGCTCGACGACACGGCCCGCGAGCGC
  GCCAAGCTGCAGATCGAGCTGGGCAAGTGCAAGGCGGAACACGACCAGCTGCTCCTCAAC
  TATGCTAAGAAGGAATCTGATCTTAATGGCGCCCAGATCAAGCTTCGAGAATATGAAGCA
  GCACTGAATTCGAAAGATGCAGCTCTTGCTACTGCACTTGGTGACAAAAAAAGTTTAGAG
  GGAGATTTGGAGGATCTGAAGGATCAGATTGCCCAGTTGGAAGCCTCCTTAGCTGCAGCC
  AAAAAACAGTTAGCAGATGAAACTTTACTTAAAGTAGATTTGGAGAATCGTTGTCAGAGC
  CTTACTGAGGACTTGGAGTTTCGCAAAAGCATGTATGAAGAGGAGATTAACGAGACCAGA
  AGGAAGCATGAAACGCGCTTGGTAGAGGTGGATTCTGGGCGTCAAATTGAGTATGAGTAC
  AAGCTGGCGCAAGCCCTTCATGAGATGAGAGAGCAACATGATGCCCAAGTGAGGCTGTAT
  AAGGAGGAGCTGGAGCAGACTTACCATGCCAAACTTGAGAATGCCAGACTGTCATCAGAG
  ATGAATACTTCTACTGTCAACAGTGCCAGGGAAGAACTGATGGAAAGCCGCATGAGAATT
  GAGAGCCTTTCATCCCAGCTTTCTAATCTACAGAAAGAGTCTAGAGCATGTTTGGAAAGG
  ATTCAAGAATTAGAGGACTTGCTTGCTAAAGAAAAAGACAACTCTCGTCGCATGCTGACA
  GACAAAGAGAGAGAGATGGCGGAAATAAGGGATCAAATGCAGCAACAGCTGAATGACTAT
  GAACAGCTTCTTGATGTAAAGTTAGCCCTGGACATGGAAATCAGTGCTTACAGGAAACTC
  TTAGAAGGCGAAGAAGAGAGGTTGAAGCTGTCTCCAAGCCCTTCTTCCCGTGTGACAGTA
  TCCCGAGCATCCTCAAGTCGTAGTGTACGTACAACTAGAGGAAAGCGGAAGAGGGTTGAT
  GTGGAAGAATCAGAGGCGAGTAGTAGTGTTAGCATCTCTCATTCCGCCTCAGCCACTGGA
  AATGTTTGCATCGAAGAAATTGATGTTGATGGGAAATTTATCCGCTTGAAGAACACTTCT
  GAACAGGATCAACCAATGGGAGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTC
  AGTTATAAATATACCTCAAGATATGTGCTGAAGGCAGGCCAGACTGTTACAATTTGGGCT
  GCAAACGCTGGTGTCACAGCCAGCCCCCCAACTGACCTCATCTGGAAGAACCAGAACTCG
  TGGGGCACTGGCGAAGATGTGAAGGTTATATTGAAAAATTCTCAGGGAGAGGAGGTTGCT
  CAAAGAAGTACAGTCTTTAAAACAACCATACCTGAAGAAGAGGAGGAGGAGGAAGAAGCA
  GCTGGAGTGGTTGTTGAGGAAGAACTTTTCCACCAGCAGGGAACCCCAAGAGCATCCAAT
  AGAAGCTGTGCAATTATGTAA
  
  Clone variation with respect to NM_005573.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005573 unedited
  NGGTGTCAACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGGAG
  ACGGCGGCGGCGCGAACCCTGCTGGGCCTCCAGTCACCCTCGTCTTGCATTTTCCCGCGT
  GCGTGTGTGAGTGGGTGTGTGTGTTTTCTTACAAAGGGTATTTCGCGATCGATCGATTGA
  TTCGTAGTTCCCCCCCGCGCGCCTTTGCCCTTTGTGCTGTAATCGAGCTCCCGCCATCCC
  AGGTGCTTCTCCGTTCCTCTAAACGCCAGCGTCTGGACGTGAGCGCAGGTCGCCGGTTTG
  TGCCTTCGGTCCCCGCTTCGCCCCCTGCCGTCCCCTCCTTATCACGGTCCCGCTCGCGGC
  CTCGCCGCCCCGCTGTCTCCGCCGCCCGCCATGGCGACTGCGACCCCCGTGCCGCCGCGG
  ATGGGCAGCCGCGCTGGCGGCCCCACCACGCCGCTGAGCCCCACGCGCCTGTCGCGGCTC
  CAGGAGAAGGAGGAGCTGCGCGAGCTCAATGACCGGCTGGCGGTGTACATCGACAAGGTG
  CGCAGCCTGGAGACGGAGAACAGCGCGCTGCAGCTGCAGGTGACGGAGCGCGAGGAGGTG
  CGCGGCCGTGAGCTCACCGGCCTCAAGGCGCTCTACGAGACCGAGCTGGCCGACGCGCGA
  CGCGCGCTCGACGACACGGCCCGCGAGCGCGCCAAGCTGCAGATCGAGCTGNGCAAGTGC
  AAGGCGGAACACGACCAGCTGCTCCTCAACTATGCTAAGAAGGAATCTGATCTTAATGGC
  GCCCAGATCAAGCTTCGAGAATATGAGCAGCACTGAATTCGAAGATGCAGCTCTTGCTAC
  TGCACTTGGTGACAAAAAAAGTTANAGGGAGATTNGGNAGATCTGAANGATCAGATTGCC
  CCATTTGGAAGCCTTCTTAGCTGCAGCCAAAAACAGTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005573 unedited
  GCTATGGACCGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTC
  CAATATTAAAAGAGCTTTTTTATTTGTTAATGTCACATAAGAAATTTAAGCAAGTTACAC
  TATCTTAAAAAACACAACGAATGCATTTTAATAGAGAAACCCTTCCCTCCCTCCACCTCC
  CTCCCCCACCCTCCTCATGAATTAAGAATCTAAGAGAAGAAGTACCCATAAAACCAAGTT
  TTGTGGAATCCATCATCCAGAGTGCTTACATGGGGATTAGGTTAATATTGCCTTCTTACA
  AAATTTCTATTTTAAAAAAAATTATAACCTTGATTGCTTATTACAAAAAAATTCAGTCCA
  AAAGTTCAATATCTTGAAAAATGCTTTTCCCCTCCCTCACAGCACCGTTTTATATATAGC
  AGAGAATAATGAAGAGATTGCTAGTCTAGATGGGGCAATCTTCAAATTACACCACGACGC
  ACAGTGGTTTATTTACCCTCCCCTCCTCCTAAGAACTTAAGAAAAAACAAAATACCCACC
  TCCAAAAAGAGTCAAAAAATTTGAGGAACCCCTTCCGAACCGTCCCCGTTATTAAGTGCA
  GTGGCAATAATTCACACCTTGCAACAAAGTGTGGGGCCTTGATTTCTTTTTACAAAACTT
  TCCGGGGCAAAAAGAGAATTAGGGCCATCAGATTTCGCACTTACTAAATCCCCTGAAGGA
  GAAATTCGAAAAATTCTCGGGCTCTAGAAAGGCCCACCCCTGTAGACGGGGAAGGGAAAC
  CCACCCTTCTTTATTTGAGAAAAGCCGGTGGAAAATTACGGGCGGGACAGATTTCTCTGG
  GGGCCGCGGGGGTCTCCGCGCGANGGAAAGGCCGTTTACAAAGACCACGAGAGGGGATGT
  CCACGCGTCAGCTCCTGAGAGCGGTGTAATGAAGAACGTGTCGCCTGAATAAGCGGCTAA
  GACGGATTGTGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005573
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005573.2, NP_005564.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 1761 bp
• Locus ID: 4001
• Cytogenetics: 5q23.2
• Domains: IF_tail, filament
• Gene Summary: 'This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.