LMO2 (NM_005574) Human Untagged Clone

CAT#: SC116662

LMO2 (untagged)-Human LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LMO2
• Synonyms: LMO-2; RBTN2; RBTNL1; RHOM2; TTG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005574 edited
  ATGGAAGGGAGCGCGGTGACTGTCCTTGAGCGCGGAGGGGCGAGCTCGCCGGCGGAGCGC
  CGGAGCAAGCGGAGGCGCAGGAGCGGCGGCGACGGCGGCGGCGGCGGCGGCGCCCGAGCA
  CCCGAGGGGGTCCGAGCCCCGGCAGCCGGCCAGCCCCGCGCCACAAAGGGAGCGCCCCCG
  CCGCCCGGCACCCCGCCTCCCTCCCCAATGTCCTCGGCCATCGAAAGGAAGAGCCTGGAC
  CCTTCAGAGGAACCAGTGGATGAGGTGCTGCAGATCCCCCCATCCCTGCTGACATGCGGC
  GGCTGCCAGCAGAACATTGGGGACCGCTACTTCCTGAAGGCCATCGACCAGTACTGGCAC
  GAGGACTGCCTGAGCTGCGACCTCTGTGGCTGCCGGCTGGGTGAGGTGGGGCGGCGCCTC
  TACTACAAACTGGGCCGGAAGCTCTGCCGGAGAGACTATCTCAGGCTTTTTGGGCAAGAC
  GGTCTCTGCGCATCCTGTGACAAGCGGATTCGTGCCTATGAGATGACAATGCGGGTGAAA
  GACAAAGTGTATCACCTGGAATGTTTCAAGTGCGCCGCCTGTCAGAAGCATTTCTGTGTA
  GGTGACAGATACCTCCTCATCAACTCTGACATAGTGTGCGAACAGGACATCTACGAGTGG
  ACTAAGATCAATGGGATGATATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005574 unedited
  GTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGCGCACA
  CCATCCCCGCGGGCGGCGCGGAGCCGGCGACAGCGCGCGAGAGGGACCGGGCGGTGGCGG
  CGGCGGGACCGGGATGGAAGGGAGCGCGGTGACTGTCCTTGAGCGCGGAGGGGCGAGCTC
  GCCGGCGGAGCGCCGGAGCAAGCGGAGGCGCAGGAGCGGCGGCGACGGCGGCGGCGGCGG
  CGGCGCCCGAGCACCCGAGGGGGTCCGAGCCCCGGCAGCCGGCCAGCCCCGCGCCACAAA
  GGGAGCGCCCCCGCCGCCCGGCACCCCGCCTCCCTCCCCAATGTCCTCGGCCATCGAAAG
  GAAGAGCCTGGACCCTTCAGAGGAACCAGTGGATGAGGTGCTGCAGATCCCCCCATCCCT
  GCTGACATGCGGCGGCTGCCAGCAGAACATTGGGGACCGCTACTTCCTGAAGGCCATCGA
  CCAGTACTGGCACGAGGACTGCCTGAGCTGCGACCTCTGTGGCTGCCGGCTGGGTGAGGT
  GGGGCGGCGCCTCTACTACAAACTGGGCCGGAAGCTCTGCCGGAGAGACTATCTCAGGCT
  TTTTGGGCAAGACGGTCTCTGCGCATCCTGTGANCAGCGGATTCGTGCCTATGANGATGA
  CATGCGGGTGAAAAGACAAGTGTATCACCTGGAATGTTTCAAGTGCGCCGCCTGTCAGAA
  GCATTCCTGTGTANGTGACAGATACCTCCTCATCCACTCTGACATAGTGTGCGAAACAGA
  CATCTACGAGTGGACTAAGATCAATGGGATGATATAGGCCCGAGTCCCCCGGCATCTTTG
  GGGAGGTGTTACTGAAGACGCCTTCTCCATGGGATCTTCCTCCTCACTCTTAGCACTTTG
  GGGTTTGAAGTGGGGTAAAGGGGATTCTTAGGGGTGGGGAAACCTTTATGGGGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005574 unedited
  ATGGCCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTCAGTCTGTCATTTTT
  ATTAAGCCTGCAGAGCTGTTTTTTTTTTCTACACACGACAAATACTTTGATATAATCTAG
  GATAATAAAATAGTTGAACAACTTTTTAAGATTTATATTTGTATAGAAACAATCTGTGGA
  ACTCCTCCCCTCAAAATGAAGGTGTCTAAAAACAGTGATTNTCATCAGCATTGCTTTAAA
  TAATTGTTTGGTTAAAAGTTGTGGTTTCCATTCTCAACCGAAATGCGTCTCCATGCAGTT
  TTCCTTGGGTCCCAAACTTAAGGCCTTGGGAAGGGGGCCAAGAAAAAAAAAGATGGCCAC
  TCTCCTTTCTGTCCTTCCCTTGTGTGGAGGACAGTGATTGAAACAGGGGAGTTAAAAAGC
  CAGGAAGAAAAGAAATCAATTGCACATCTCTAGTTCGCAAGCGTCAAAGTCACAACAAGT
  CTGTACACAACAACTCTCTATCTGTAGATATGAAATTCCATCATGATAGCACAGCGCCTG
  CTTGCCCCTAAATGTTCCTTTCTTCTGCTAATCATGTCAGTTACTATGGATGGGCTGTGG
  GCCATAAGTCTCCCTCAAGGGCTGGTCCTTCTGTCACCTTGAATGTCAGCCCCTGAATTA
  TGCACTTGATGCTATGTCTGATACCCATAAGTTTACCATCCCCTAGAATCCCTACCCCAC
  CTCACCCCCAAAGTGCTAAAAGTGAGACCAGATGCATGGANCGGGGTTTTAGGAACACCT
  CCCAAGATGCGGGGACTGGGCCTTTTCATCCATGGTCTAATCACTCTAAATGTCTGTTCC
  CCCTTTGTCAAGTGTGAGGAGGTTTTGCACTCACAAAATGCTTTGAAGGGGGCCTTAAAA
  TTCAGGGAACTTTGTTTCCCCCATGTATTTATAGGCAATCCTTTTCAGGATGCAAAACCT
  TGCCAAAACTAAATTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005574
• Insert Size: 1600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005574.2, NP_005565.1
• RefSeq Size: 2303 bp
• RefSeq ORF: 477 bp
• Locus ID: 4005
• Cytogenetics: 11p13
• Domains: LIM
• Protein Families: Druggable Genome
• Gene Summary: 'LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform.