LMO2 (NM_005574) Human Untagged Clone

CAT#: SC116662

LMO2 (untagged)-Human LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 1


  "NM_005574" in other vectors (11)

Reconstitution Protocol

USD 420.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LMO2
Synonyms LMO-2; RBTN2; RBTNL1; RHOM2; TTG2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_005574 edited
ATGGAAGGGAGCGCGGTGACTGTCCTTGAGCGCGGAGGGGCGAGCTCGCCGGCGGAGCGC
CGGAGCAAGCGGAGGCGCAGGAGCGGCGGCGACGGCGGCGGCGGCGGCGGCGCCCGAGCA
CCCGAGGGGGTCCGAGCCCCGGCAGCCGGCCAGCCCCGCGCCACAAAGGGAGCGCCCCCG
CCGCCCGGCACCCCGCCTCCCTCCCCAATGTCCTCGGCCATCGAAAGGAAGAGCCTGGAC
CCTTCAGAGGAACCAGTGGATGAGGTGCTGCAGATCCCCCCATCCCTGCTGACATGCGGC
GGCTGCCAGCAGAACATTGGGGACCGCTACTTCCTGAAGGCCATCGACCAGTACTGGCAC
GAGGACTGCCTGAGCTGCGACCTCTGTGGCTGCCGGCTGGGTGAGGTGGGGCGGCGCCTC
TACTACAAACTGGGCCGGAAGCTCTGCCGGAGAGACTATCTCAGGCTTTTTGGGCAAGAC
GGTCTCTGCGCATCCTGTGACAAGCGGATTCGTGCCTATGAGATGACAATGCGGGTGAAA
GACAAAGTGTATCACCTGGAATGTTTCAAGTGCGCCGCCTGTCAGAAGCATTTCTGTGTA
GGTGACAGATACCTCCTCATCAACTCTGACATAGTGTGCGAACAGGACATCTACGAGTGG
ACTAAGATCAATGGGATGATATAG
>OriGene 5' read for NM_005574 unedited
GTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGCGCACA
CCATCCCCGCGGGCGGCGCGGAGCCGGCGACAGCGCGCGAGAGGGACCGGGCGGTGGCGG
CGGCGGGACCGGGATGGAAGGGAGCGCGGTGACTGTCCTTGAGCGCGGAGGGGCGAGCTC
GCCGGCGGAGCGCCGGAGCAAGCGGAGGCGCAGGAGCGGCGGCGACGGCGGCGGCGGCGG
CGGCGCCCGAGCACCCGAGGGGGTCCGAGCCCCGGCAGCCGGCCAGCCCCGCGCCACAAA
GGGAGCGCCCCCGCCGCCCGGCACCCCGCCTCCCTCCCCAATGTCCTCGGCCATCGAAAG
GAAGAGCCTGGACCCTTCAGAGGAACCAGTGGATGAGGTGCTGCAGATCCCCCCATCCCT
GCTGACATGCGGCGGCTGCCAGCAGAACATTGGGGACCGCTACTTCCTGAAGGCCATCGA
CCAGTACTGGCACGAGGACTGCCTGAGCTGCGACCTCTGTGGCTGCCGGCTGGGTGAGGT
GGGGCGGCGCCTCTACTACAAACTGGGCCGGAAGCTCTGCCGGAGAGACTATCTCAGGCT
TTTTGGGCAAGACGGTCTCTGCGCATCCTGTGANCAGCGGATTCGTGCCTATGANGATGA
CATGCGGGTGAAAAGACAAGTGTATCACCTGGAATGTTTCAAGTGCGCCGCCTGTCAGAA
GCATTCCTGTGTANGTGACAGATACCTCCTCATCCACTCTGACATAGTGTGCGAAACAGA
CATCTACGAGTGGACTAAGATCAATGGGATGATATAGGCCCGAGTCCCCCGGCATCTTTG
GGGAGGTGTTACTGAAGACGCCTTCTCCATGGGATCTTCCTCCTCACTCTTAGCACTTTG
GGGTTTGAAGTGGGGTAAAGGGGATTCTTAGGGGTGGGGAAACCTTTATGGGGGTT
>OriGene 3' read for NM_005574 unedited
ATGGCCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTCAGTCTGTCATTTTT
ATTAAGCCTGCAGAGCTGTTTTTTTTTTCTACACACGACAAATACTTTGATATAATCTAG
GATAATAAAATAGTTGAACAACTTTTTAAGATTTATATTTGTATAGAAACAATCTGTGGA
ACTCCTCCCCTCAAAATGAAGGTGTCTAAAAACAGTGATTNTCATCAGCATTGCTTTAAA
TAATTGTTTGGTTAAAAGTTGTGGTTTCCATTCTCAACCGAAATGCGTCTCCATGCAGTT
TTCCTTGGGTCCCAAACTTAAGGCCTTGGGAAGGGGGCCAAGAAAAAAAAAGATGGCCAC
TCTCCTTTCTGTCCTTCCCTTGTGTGGAGGACAGTGATTGAAACAGGGGAGTTAAAAAGC
CAGGAAGAAAAGAAATCAATTGCACATCTCTAGTTCGCAAGCGTCAAAGTCACAACAAGT
CTGTACACAACAACTCTCTATCTGTAGATATGAAATTCCATCATGATAGCACAGCGCCTG
CTTGCCCCTAAATGTTCCTTTCTTCTGCTAATCATGTCAGTTACTATGGATGGGCTGTGG
GCCATAAGTCTCCCTCAAGGGCTGGTCCTTCTGTCACCTTGAATGTCAGCCCCTGAATTA
TGCACTTGATGCTATGTCTGATACCCATAAGTTTACCATCCCCTAGAATCCCTACCCCAC
CTCACCCCCAAAGTGCTAAAAGTGAGACCAGATGCATGGANCGGGGTTTTAGGAACACCT
CCCAAGATGCGGGGACTGGGCCTTTTCATCCATGGTCTAATCACTCTAAATGTCTGTTCC
CCCTTTGTCAAGTGTGAGGAGGTTTTGCACTCACAAAATGCTTTGAAGGGGGCCTTAAAA
TTCAGGGAACTTTGTTTCCCCCATGTATTTATAGGCAATCCTTTTCAGGATGCAAAACCT
TGCCAAAACTAAATTTTC
Restriction Sites NotI-NotI     
ACCN NM_005574
Insert Size 1600 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005574.2, NP_005565.1
RefSeq Size 2303 bp
RefSeq ORF 477 bp
Locus ID 4005
Cytogenetics 11p13
Domains LIM
Protein Families Druggable Genome
Gene Summary 'LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2008]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform.

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