SHIP (INPP5D) (NM_005541) Human Untagged Clone

CAT#: SC116675

INPP5D (untagged)-Human inositol polyphosphate-5-phosphatase, 145kDa (INPP5D), transcript variant 2


  "NM_005541" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol INPP5D
Synonyms hp51CN; p150Ship; SHIP; SHIP-1; SHIP1; SIP-145
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116675 sequence for NM_005541 edited (data generated by NextGen Sequencing)
ATGGTCCCCTGCTGGAACCATGGCAACATCACCCGCTCCAAGGCGGAGGAGCTGCTTTCC
AGGACAGGCAAGGACGGGAGCTTCCTCGTGCGTGCCAGCGAGTCCATCTCCCGGGCATAC
GCGCTCTGCGTGCTGTATCGGAATTGCGTTTACACTTACAGAATTCTGCCCAATGAAGAT
GATAAATTCACTGTTCAGGCATCCGAAGGCGTCTCCATGAGGTTCTTCACCAAGCTGGAC
CAGCTCATCGAGTTTTACAAGAAGGAAAACATGGGGCTGGTGACCCATCTGCAATACCCT
GTGCCGCTGGAGGAAGAGGACACAGGCGACGACCCTGAGGAGGACACAGAAAGTGTCGTG
TCTCCACCCGAGCTGCCCCCAAGAAACATCCCGCTGACTGCCAGCTCCTGTGAGGCCAAG
GAGGTTCCTTTTTCAAACGAGAATCCCCGAGCGACCGAGACCAGCCGGCCGAGCCTCTCC
GAGACATTGTTCCAGCGACTGCAAAGCATGGACACCAGTGGGCTTCCAGAAGAGCATCTT
AAGGCCATCCAAGATTATTTAAGCACTCAGCTCGCCCAGGACTCTGAATTTGTGAAGACA
GGGTCCAGCAGTCTTCCTCACCTGAAGAAACTGACCACACTGCTCTGCAAGGAGCTCTAT
GGAGAAGTCATCCGGACCCTCCCATCCCTGGAGTCTCTGCAGAGGTTATTTGACCAGCAG
CTCTCCCCGGGCCTCCGTCCACGTCCTCAGGTTCCTGGTGAGGCCAATCCCATCAACATG
GTGTCCAAGCTCAGCCAACTGACAAGCCTGTTGTCATCCATTGAAGACAAGGTCAAGGCC
TTGCTGCACGAGGGTCCTGAGTCTCCGCACCGGCCCTCCCTTATCCCTCCAGTCACCTTT
GAGGTGAAGGCAGAGTCTCTGGGGATTCCTCAGAAAATGCAGCTCAAAGTCGACGTTGAG
TCTGGGAAACTGATCATTAAGAAGTCCAAGGATGGTTCTGAGGACAAGTTCTACAGCCAC
AAGAAAATCCTGCAGCTCATTAAGTCACAGAAATTTCTGAATAAGTTGGTGATCTTGGTG
GAAACAGAGAAGGAGAAGATCCTGCGGAAGGAATATGTTTTTGCTGACTCCAAAAAGAGA
GAAGGCTTCTGCCAGCTCCTGCAGCAGATGAAGAACAAGCACTCAGAGCAGCCGGAGCCC
GACATGATCACCATCTTCATCGGCACCTGGAACATGGGTAACGCCCCCCCTCCCAAGAAG
ATCACGTCCTGGTTTCTCTCCAAGGGGCAGGGAAAGACGCGGGACGACTCTGCGGACTAC
ATCCCCCATGACATTTACGTGATCGGCACCCAAGAGGACCCCCTGAGTGAGAAGGAGTGG
CTGGAGATCCTCAAACACTCCCTGCAAGAAATCACCAGTGTGACTTTTAAAACAGTCGCC
ATCCACACGCTCTGGAACATCCGCATCGTGGTGCTGGCCAAGCCTGAGCACGAGAACCGG
ATCAGCCACATCTGTACTGACAACGTGAAGACAGGCATTGCAAACACACTGGGGAACAAG
GGAGCCGTGGGGGTGTCGTTCATGTTCAATGGAACCTCCTTAGGGTTCGTCAACAGCCAC
TTGACTTCAGGAAGTGAAAAGAAACTCAGGCGAAACCAAAACTATATGAACATTCTCCGG
TTCCTGGCCCTGGGCGACAAGAAGCTGAGTCCCTTTAACATCACTCACCGCTTCACGCAC
CTCTTCTGGTTTGGGGATCTTAACTACCGTGTGGATCTGCCTACCTGGGAGGCAGAAACC
ATCATCCAGAAAATCAAGCAGCAGCAGTACGCAGACCTCCTGTCCCACGACCAGCTGCTC
ACAGAGAGGAGGGAGCAGAAGGTCTTCCTACACTTCGAGGAGGAAGAAATCACGTTTGCC
CCAACCTACCGTTTTGAGAGACTGACTCGGGACAAATACGCCTACACCAAGCAGAAAGCG
ACAGGGATGAAGTACAACTTGCCTTCCTGGTGTGACCGAGTCCTCTGGAAGTCTTATCCC
CTGGTGCACGTGGTGTGTCAGTCTTATGGCAGTACCAGCGACATCATGACGAGTGACCAC
AGCCCTGTCTTTGCCACATTTGAGGCAGGAGTCACTTCCCAGTTTGTCTCCAAGAACGGT
CCCGGGACTGTTGACAGCCAAGGACAGATTGAGTTTCTCAGGTGCTATGCCACATTGAAG
ACCAAGTCCCAGACCAAATTCTACCTGGAGTTCCACTCGAGCTGCTTGGAGAGTTTTGTC
AAGAGTCAGGAAGGAGAAAATGAAGAAGGAAGTGAGGGGGAGCTGGTGGTGAAGTTTGGT
GAGACTCTTCCAAAGCTGAAGCCCATTATCTCTGACCCTGAGTACCTGCTAGACCAGCAC
ATCCTCATCAGCATCAAGTCCTCTGACAGCGACGAATCCTATGGCGAGGGCTGCATTGCC
CTTCGGTTAGAGGCCACAGAAACGCAGCTGCCCATCTACACGCCTCTCACCCACCATGGG
GAGTTGACAGGCCACTTCCAGGGGGAGATCAAGCTGCAGACCTCTCAGGGCAAGACGAGG
GAGAAGCTCTATGACTTTGTGAAGACGGAGCGTGATGAATCCAGTGGGCCAAAGACCCTG
AAGAGCCTCACCAGCCACGACCCCATGAAGCAGTGGGAAGTCACTAGCAGGGCCCCTCCG
TGCAGTGGCTCCAGCATCACTGAAATCATCAACCCCAACTACATGGGAGTGGGGCCCTTT
GGGCCACCAATGCCCCTGCACGTGAAGCAGACCTTGTCCCCTGACCAGCAGCCCACAGCC
TGGAGCTACGACCAGCCGCCCAAGGACTCCCCGCTGGGGCCCTGCAGGGGAGAAAGTCCT
CCGACACCTCCCGGCCAGCCGCCCATATCACCCAAGAAGTTTTTACCCTCAACAGCAAAC
CGGGGTCTCCCTCCCAGGACACAGGAGTCAAGGCCCAGTGACCTGGGGAAGAACGCAGGG
GACACGCTGCCTCAGGAGGACCTGCCGCTGACGAAGCCCGAGATGTTTGAGAACCCCCTG
TATGGGTCCCTGAGTTCCTTCCCTAAGCCTGCTCCCAGGAAGGACCAGGAATCCCCCAAA
ATGCCGCGGAAGGAACCCCCGCCCTGCCCGGAACCCGGCATCTTGTCGCCCAGCATCGTG
CTCACCAAAGCCCAGGAGGCTGATCGCGGCGAGGGGCCCGGCAAGCAGGTGCCCGCGCCC
CGGCTGCGCTCCTTCACGTGCTCATCCTCTGCCGAGGGCAGGGCGGCCGGCGGGGACAAG
AGCCAAGGGAAGCCCAAGACCCCGGTCAGCTCCCAGGCCCCGGTGCCGGCCAAGAGGCCC
ATCAAGCCTTCCAGATCGGAAATCAACCAGCAGACCCCGCCCACCCCGACGCCGCGGCCG
CCGCTGCCAGTCAAGAGCCCGGCGGTGCTGCACCTCCAGCACTCCAAGGGCCGCGACTAC
CGCGACAACACCGAGCTCCCGCATCACGGCAAGCACCGGCCGGAGGAGGGGCCACCAGGG
CCTCTAGGCAGGACTGCCATGCAGTGA

Clone variation with respect to NM_005541.3
>OriGene 5' read for NM_005541 unedited
TCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
GCACGAGGGTGGCAGCAGCCGAGGCCACCAAGAGGCAACGGGCGGCAGGTTGCAGTGGAG
GGGCCTCCGCTCCCCTCGGTGGTGTGTGGGTCCTGGGGGTGCCTGCCGGCCCGGCCGAGG
AGGCCCACGCCCACCATGGTCCCCTGCTGGAACCATGGCAACATCACCCGCTCCAAGGCG
GAGGAGCTGCTTTCCAGGACAGGCAAGGACGGGAGCTTCCTCGTGCGTGCCAGCGAGTCC
ATCTCCCGGGCATACGCGCTCTGCGTGCTGTATCGGAATTGCGTTTACACTTACAGAATT
CTGCCCAATGAAGATGATAAATTCACTGTTCAGGCATCCGAAGGCGTCTCCATGAGGTTC
TTCACCAAGCTGGACCAGCTCATCGAGTTTTACAAGAAGGAAAACATGGNGCTGGTGACC
CATCTGCATACCCTGTGCCGCTGGAGGAAGAGGACACAGGCGACGACCCCTGAGAGGACA
CAGAAAGTGTCGTGTCTTCACCCGAGCTGCCCCCCAGAAACATCCCGCTGACTGCCAGCT
CCTGTGAGGCCACGAGGGTNCTTTTTCAAACGAGATCCCCGAGCGACCGAGACCANCGGC
CGAGGCTTTTCGAGAATTGTTCCAGCGACTGCAAGCATGGCCCCAGGGGGCTTCCGCAGA
CATTTAAGGGCCCCCCAGATATTTAAGCCTTCGTTTGCCCAGAATTTGAATTTTGAGAAC
AGGTCACCAGTTTTCTCACCTGAAAAATGACCCACTGTTTGTAGGGGTCTTTGGGAAAAT
TCCGGCCCTCCACTCCTGGGTTCTCCGAGGTATTTACCAAACTTTCCCGGCCC
Restriction Sites NotI-NotI     
ACCN NM_005541
Insert Size 5500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005541.3, NP_005532.2
RefSeq Size 4925 bp
RefSeq ORF 3567 bp
Locus ID 3635
Cytogenetics 2q37.1
Domains SH2, Exo_endo_phos
Protein Families Druggable Genome
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, Phosphatidylinositol signaling system
Gene Summary 'This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Deficiencies in the encoded protein, SHIP1, have been associated with Inflammatory Bowel Disease types such as Crohn's Disease and Ulcerative Colitis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2020]'
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is 1 aa shorter than isoform a.

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