NIT1 (NM_005600) Human Untagged Clone

CAT#: SC116684

NIT1 (untagged)-Human nitrilase 1 (NIT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NIT1
• Synonyms: MGC57670
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116684 sequence for NM_005600 edited (data generated by NextGen Sequencing)
  ATGCTGGGCTTCATCACCAGGCCTCCTCACAGATTCCTGTCCCTTCTGTGTCCTGGACTC
  CGGATACCTCAACTCTCAGTACTTTGTGCTCAGCCCAGGCCCAGAGCCATGGCTATCTCC
  TCTTCCTCCTGCGAACTGCCCCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAG
  CAACAGAACTTTAAAACATGTGCTGAGCTGGTTCGAGAGGCTGCCAGACTGGGTGCCTGC
  CTGGCTTTCCTGCCTGAGGCATTTGACTTCATTGCACGGGACCCTGCAGAGACGCTACAC
  CTGTCTGAACCACTGGGTGGGAAACTTTTGGAAGAATACACCCAGCTTGCCAGGGAATGT
  GGACTCTGGCTGTCCTTGGGTGGTTTCCATGAGCGTGGCCAAGACTGGGAGCAGACTCAG
  AAAATCTACAATTGTCACGTGCTGCTGAACAGCAAAGGGGCAGTAGTGGCCACTTACAGG
  AAGACACATCTGTGTGACGTAGAGATTCCAGGGCAGGGGCCTATGTGTGAAAGCAACTCT
  ACCATGCCTGGGCCCAGTCTTGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTA
  GCTGTCTGCTATGACATGCGGTTCCCTGAACTCTCTCTGGCATTGGCTCAAGCTGGAGCA
  GAGATACTTACCTATCCTTCAGCTTTTGGATCCATTACAGGCCCAGCCCACTGGGAGGTG
  TTGCTGCGGGCCCGTGCTATCGAAACCCAGTGCTATGTAGTGGCAGCAGCACAGTGTGGA
  CGCCACCATGAGAAGAGAGCAAGTTATGGCCACAGCATGGTGGTAGACCCCTGGGGAACA
  GTGGTGGCCCGCTGCTCTGAGGGGCCAGGCCTCTGCCTTGCCCGAATAGACCTCAACTAT
  CTGCGACAGTTGCGCCGACACCTGCCTGTGTTCCAGCACCGCAGGCCTGACCTCTATGGC
  AATCTGGGTCACCCACTGTCTTAA
  
  Clone variation with respect to NM_005600.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005600 unedited
  GTAATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTCCATCTTCCCACTGT
  ATGGTCTTGTCCCTTAGCCTATAATTTCTTATAAAGAGCGTTTTGAAGACTAGGTTTTTA
  CTGGCAGGGCCCTGATTCAGGCTCACAGTATAAAGAGAGAAGTCAGAGAATCCTAGAAAT
  TTATTGCTTGAAGAAGGGGTTGGTGTCCTCATATCTCACCTTCCTCAGGCTGGGCTTCAT
  CACCAGGCCTCCTCACAGATTCCTGTCCCTTCTGTGTCCTGGACTCCGGATACCTCAACT
  CTCAGTACTTTGTGCTCAGCCCAGGCCCAGAGCCATGGCTATCTCCTCTTCCTCCTGCGA
  ACTGCCCCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAGCAACAGAACTTTAA
  AACATGTGCTGAGCTGGTTCGAGAGGCTGCCAGACTGGGTGCCTGCCTGGCTTTCCTGCC
  TGAGGCATTTGACTTCATTGCACGGGACCCTGCAGAGACGCTACACCTGTCTGAACCACT
  GGGTGGGAAACTTTTGGAAGAATACACCCAGCTTGCCAGGGAATGTGGACTCTGGCTGTC
  CTTGGGTGGTTTCCATGAGCGTGGCCAAGACTGGGAGCAGACTCAGAAAATCTACAATTG
  TCACGTGCTGCTGAACAGCAAAGGGGCAGTAGTGGCCACTTACAGGAAGACACATCTGTG
  TGACGTAGAGATTCCAGGGCAGGNNGCCTATGTGTGAAAGCAACTCTACCATGCCTGGGC
  CCAGTCTTGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTAGCTGTCTGCTATG
  ACATGCNGNTCCCCTGACTCTCTCTGGCATTGGCTCAAGCTGGACAGAGATACTTACCTA
  TCCTTCACTTTTNGGATCCATACAGGNCCCAGCACTGGGGGAGTGTTGCTGGGGGCCCGT
  GCTATCNAACCCANTGCTTTGTATGCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005600 unedited
  CGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTGATTATTATATTTTTCAATGC
  CAGTGCTGCTCAGCCCTCAGCAGAACTTCAGTTTCCATGAAATAAACAATGACTATATAA
  AATTCCACCTTTCTGAAACTGCAGTCTGACCTCAGGTGAAGCTCAAGTGAAAGTTTCTTT
  CCCAAGCAGCCCATCTGTGTTCCATCAAGAGAGTCAAGGTTCTCCAAGTGAGGGGAGCTG
  TGCCTGGATCCTGCCTCCAAGTCACATGAGCACTAGCTCATATTGGCAGGGTGGGGGTGG
  GAGGGGCAGGTCTAAACTCACAGAAGTCAAGTCTTAAGACAGTGGGTGACCCAGATTGCC
  ATAGAGGTCAAGCCTGCGGTGCTGGAACACAGGCAGGTGTCGGCGCAACTGTCGCAGATA
  GTTGAGGTCTATTCGGGCAAAGCAGAGGCCTGGCCCCTCAGAGCAGCGGGCCACCACTGT
  TCCCCAGGGGTCTACCACCATGCTGAGGCCATAACTTGCTCTCTTTTATGGGGGCGTCCA
  CACTGTGCTGCTGCACTACTATCACTGGGTTTCGATACACGGGCCCGAGCAACACCTTCC
  AGGGGCTGGGCCTGAATGGATCCAAAGCCTGAGGATAGGTAGTATCTCTGCTCCATTTGA
  GCCATGCCAAAAAGAGTTCAGGAACCGCTTGCCTTTCAGACAGTTAACAATCTTGCCTGC
  TGTGTGCTCGCAGGGGACTAAAAATGGCCCATGCATGGACAATTGCTTTACACATAGCCC
  CCGCCCCGGAACCCTACCCAAAACATTGTTTTCCTTTATGGCCCCCCGCCCCTTTCTGCC
  ACACCCCCCCAATGGTTTTTCGCCCTTCCCCCCCTGGCCCTCTCTGATTCACCAGGACTC
  TCTCCCTCTTCCTGGAACGGGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005600
• Insert Size: 1580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005600.1, NP_005591.1
• RefSeq Size: 1385 bp
• RefSeq ORF: 984 bp
• Locus ID: 4817
• Cytogenetics: 1q23.3
• Domains: CN_hydrolase
• Gene Summary: 'This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1).