Cofilin 1 (CFL1) (NM_005507) Human Untagged Clone

CAT#: SC116694

CFL1 (untagged)-Human cofilin 1 (non-muscle) (CFL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CFL1
• Synonyms: CFL; cofilin; HEL-S-15
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005507 edited
  GAATTCGGCACGAGGCTGCAGCGCTCTCGTCTTCTGCGGCTCTCGGTGCCCTCTCCTTTT
  CGTTTCCGGAAACATGGCCTCCGGTGTGGCTGTCTCTGATGGTGTCATCAAGGTGTTCAA
  CGACATGAAGGTGCGTAAGTCTTCAACGCCAGAGGAGGTGAAGAAGCGCAAGAAGGCGGT
  GCTCTTCTGCCTGAGTGAGGACAAGAAGAACATCATCCTGGAGGAGGGCAAGGAGATCCT
  GGTGGGCGATGTGGGCCAGACTGTCGACGATCCCTACGCCACCTTTGTCAAGATGCTGCC
  AGATAAGGACTGCCGCTATGCCCTCTATGATGCAACCTATGAGACCAAGGAGAGCAAGAA
  GGAGGATCTGGTGTTTATCTTCTGGGCCCCCGAGTCTGCGCCCCTTAAGAGCAAAATGAT
  TTATGCCAGCTCCAAGGACGCCATCAAGAAGAAGCTGACAGGGATCAAGCATGAATTGCA
  AGCAAACTGCTACGAGGAGGTCAAGGACCGCTGCACCCTGGCAGAGAAGCTGGGGGGCAG
  TGCCGTCATCTCCCTGGAGGGCAAGCCTTTGTGAGCCCCTTCTGGCCCCCTGCCTGGAGC
  ATCTGGCAGCCCCACACCTGCCCTTGGGGGTTGCAGGCTGCCCCCTTCCTGCCAGACCGG
  AGGGGCTGGGGGGATCCCAGCAGGGGGAGGGCAATCCCTTCACCCCAGTTGCCAAACAGA
  CCCCCCACCCCCTGGATTTTCCTTCTCCCTCCATCCCTTGACGGTTCTGGCCTTCCCAAA
  CTGCTTTTGATCTTTTGATTCCTCTTGGGCTGAAGCAGACCAAGTTCCCCCCAGGCACCC
  CAGTTGTGGGGGAGCCTGTATTTTTTTTAACAACATCCCCATTCCCCACCTGGTCCTCCC
  CCTTCCCATGCTGCCAACTTCTAACCGCAATAGTGACTCTGTGCTTGTCTGTTTAGTTCT
  GTGTATAAATGGAATGTTGTGGAGATGACCCCTCCCTGTGCCGGCTGGTTCCTCTCCCTT
  TTCCCCTGGTCACGGCTACTCATGGAAGCAGGACCAGTAAGGGACCTTCGATTAAAAAAA
  AAAAAGACAATAATAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005507 unedited
  NGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCTGCAGCG
  CTCTCGTCTTCTGCGGCTCTCGGTGCCCTCTCCTTTTCGTTTCCGGAAACATGGCCTCCG
  GTGTGGCTGTCTCTGATGGTGTCATCAAGGTGTTCAACGACATGAAGGTGCGTAAGTCTT
  CAACGCCAGAGGAGGTGAAGAAGCGCAAGAAGGCGGTGCTCTTCTGCCTGAGTGAGGACA
  AGAAGAACATCATCCTGGAGGAGGGCAAGGAGATCCTGGTGGGCGATGTGGGCCAGACTG
  TCGACGATCCCTACGCCACCTTTGTCAAGATGCTGCCAGATAAGGACTGCCGCTATGCCC
  TCTATGATGCAACCTATGAGACCAAGGAGAGCAAGAAGGAGGATCTGGTGTTTATCTTCT
  GGGCCCCCGAGTCTGCGCCCCTTAAGAGCAAAATGATTTATGCCAGCTCCAAGGACGCCA
  TCAAGAAGAAGCTGACAGGGATCAAGCATGAATTGCAAGCAAACTGCTACGAGGAGGTCA
  AGGACCGCTGCACCCTGGCAGAGAAGCTGGGGGGCAGTGCCGTCATCTCCCTGGAGGGCA
  AGCCTTTGTGAGCCCCTTCTGGCCCCCTGCCTGGAGCATCTGGCAGCCCCACACCTGCCC
  TTGGGGGTTGCAGGCTGCCCCCTTCCTGCCAGACCGGAGGGGCTGGGGGGATCCCAGCAG
  GGGGAGGGCAATCCCTTCACCCCAGTTGCCAAACAGACCCCCCCACCCCTGGATTNTCCT
  TCTCCCTTCATNNCCTTGACGTTCTGGCCTTTCCAAACTGCNTTTGATCTTTTGATTCCT
  CTTGNNGCTGAGCAGACCAAGTTCCCCCCCAGCACCCCANTGTGGGGGAGCCTGTATTTT
  TTTAACACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005507 unedited
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTATTGGCTTTTTTTTTTT
  TAATCGAAGGTCCCTTACTGGTCCTGCTTCCATGAGTAGCCGTGACCAGGGGAAAAGGGA
  GAGGAACCAGCCGGCACAGGGAGGGGTCATCTCCACAACATTCCATTTATACACAGAACT
  AAACAGACAAGCACAGAGTCACTATTGCGGTTAAAAGTTGGCAGCATGGGAAGGGGGAGG
  ACCAGGTGGGGAATGGGGATGTTGTTAAAAAAAATACAGGCTCCCCCACAACTGGGGTGC
  CTGGGGGGAACTTGGTCTGCTTCAGCCCAAGAGGAATCAAAAGATCAAAAGCAGTTTGGG
  AAGGCCAGAACCGTCAAGGGATGGAGGGAGAAGGAAAATCCAGGGGGTGGGGGGTCTGTT
  TGGCAACTGGGGTGAAGGGATTGCCCTCCCCCTGCTGGGATCCCCCCAGCCCCTCCGGTC
  TGGCAGGAAGGGGGCAGCCTGCAACCCCCAAGGGCAGGTGTGGGGCTGCCAGATGCTCCA
  GGCAGGGGGCCAGAAGGGGCTCACAAAGGCTTGCCCTCCAGGGAGATGACGGCACTGCCC
  CCCAGCTTCTCTGCCAGGGTGCAGCGGTCCTTGACCTCCTCGTAGCAGTTTGCTTGCAAT
  TCATGCTTGATCCCTGTCAGCTTCTTCTTGATGGCGTCCTTGGAGCTGGCATAAATCATT
  TTGCTCTTAAGGGGCGCAGACTCGGGGGCCCCAGAAGAAAACACCAGATCCTNCTTCTTG
  CTCTNCTGGNCTCATANGTTGCATCATAAAGGGCATAGCGGCAGTCCCTATCTGGCAGCA
  TCTTGACAAAGTGGGCGTAAGGAACGTNAAACGTCTGGGCCAAATGGGCAACCAGGACTC
  CTTGCCCTCTCCAGAAAGAGGTCTTCTTTGCCTACTCAGGCAAAGAGACCCGCTTCTGCC
  CTCTCACCCCTTTGGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005507
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005507.2, NP_005498.1
• RefSeq Size: 1260 bp
• RefSeq ORF: 501 bp
• Locus ID: 1072
• Cytogenetics: 11q13.1
• Domains: ADF
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]'