IFI16 (NM_005531) Human Untagged Clone

CAT#: SC116712

IFI16 (untagged)-Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2


  "NM_005531" in other vectors (6)

Reconstitution Protocol

USD 540.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol IFI16
Synonyms IFNGIP1; PYHIN2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_005531 edited
GAATTCGGCACGAGGGAATAGGAGCAAGCCAGCACTAGTCAGCTAACTAAGTGACTCAAC
CAAGGCCTTTTTTCCTTGTTATCTTTGCAGATACTTCATTTTCTTAGCGTTTCTGGAGAT
TACAACATCCTGCGGTTCCGTTTCTGGGAACTTTACTGATTTATCTCCCCCCTCACACAA
ATAAGCATTGATTCCTGCATTTCTGAAGATCTCAAGATCTGGACTACTGTTGAAAAAATT
TCCAGTGAGGCTCACTTATGTCTGTAAAGATGGGAAAAAAATACAAGAACATTGTTCTAC
TAAAAGGATTAGAGGTCATCAATGATTATCATTTTAGAATGGTTAAGTCCTTACTGAGCA
ACGATTTAAAACTTAATTTAAAAATGAGAGAAGAGTATGACAAAATTCAGATTGCTGACT
TGATGGAAGAAAAGTTCCGAGGTGATGCTGGTTTGGGCAAACTAATAAAAATTTTCGAAG
ATATACCAACGCTTGAAGACCTGGCTGAAACTCTTAAAAAAGAAAAGTTAAAAGTAAAAG
GACCAGCCCTATCAAGAAAGAGGAAGAAGGAAGTGGATGCTACTTCACCTGCACCCTCCA
CAAGCAGCACTGTCAAAACTGAAGGAGCAGAGGCAACTCCTGGAGCTCAGAAAAGAAAAA
AATCAACCAAAGAAAAGGCTGGACCCAAAGGGAGTAAGGTGTCCGAGGAACAGACTCAGC
CTCCCTCTCCTGCAGGAGCCGGCATGTCCACAGCCATGGGCCGTTCCCCATCTCCCAAGA
CCTCATTGTCAGCTCCACCCAACACTTCTTCAACTGAGAACCCGAAAACAGTGGCCAAAT
GTCAGGTAACTCCCAGAAGAAATGTTCTCCAAAAACGCCCAGTGATAGTGAAGGTACTGA
GTACAACAAAGCCATTTGAATATGAGACCCCAGAAATGGAGAAAAAAATAATGTTTCATG
CTACAGTGGCTACACAGACACAGTTCTTCCATGTGAAGGTTTTAAACACCAGCTTGAAGG
AGAAATTCAATGGAAAGAAAATCATCATCATATCAGATTATTTGGAATATGATAGTCTCC
TAGAGGTCAATGAAGAATCTACTGTATCTGAAGCTGGTCCTAACCAAACGTTTGAGGTTC
CAAATAAAATCATCAACAGAGCAAAGGAAACTCTGAAGATTGATATTCTTCACAAACAAG
CTTCAGGAAATATTGTATATGGGGTATTTATGCTACATAAGAAAACAGTAAATCAGAAGA
CCACAATCTACGAAATTCAGGATGATAGAGGAAAAATGGATGTAGTGGGGACAGGACAAT
GTCACAATATCCCCTGTGAAGAAGGAGATAAGCTCCAACTTTTCTGCTTTCGACTTAGAA
AAAAGAACCAGATGTCAAAACTGATTTCAGAAATGCATAGTTTTATCCAGATAAAGAAAA
AAACAAACCCGAGAAACAATGACCCCAAGAGCATGAAGCTACCCCAGGAACAGAGTCAGC
TTCCAAATCCTTCAGAGGCCAGCACAACCTTCCCTGAGAGCCATCTTCGGACTCCTCAGA
TGCCACCAACAACTCCATCCAGCAGTTTCTTCACCAAGAAAAGTGAAGACACAATCTCCA
AAATGAATGACTTCATGAGGATGCAGATACTGAAGGAAGGGAGTCATTTTCCAGGACCGT
TCATGACCAGCATAGGCCCAGCTGAGAGCCATCCCCACACTCCTCAGATGCCTCCATCAA
CACCAAGCAGCAGTTTCTTAACCACGTTGAAACCAAGACTGAAGACTGAACCTGAAGAAG
TTTCCATAGAAGACAGTGCCCAGAGTGACCTCAAAGAAGTGATGGTGCTGAACGCAACAG
AATCATTTGTATATGAGCCCAAAGAGCAGAAGAAAATGTTTCATGCCACAGTGGCAACTG
AGAATGAAGTCTTCCGAGTGAAGGTTTTTAATATTGACCTAAAGGAGAAGTTCACCCCAA
AGAAGATCATTGCCATAGCAAATTATGTTTGCCGCAATGGGTTCCTGGAGGTATATCCTT
TCACACTTGTGGCTGATGTGAATGCTGACCGAAACATGGAGATCCCAAAAGGATTGATTA
GAAGTGCCAGCGTAACTCCTAAAATCAATCAGCTTTGCTCACAAACTAAAGGAAGTTTTG
TGAATGGGGTGTTTGAGGTACATAAGAAAAATGTAAGGGGTGAATTCACTTATTATGAAA
TACAAGATAATACAGGGAAGATGGAAGTGGTGGTGCATGGACGACTGACCACAATCAACT
GTGAGGAAGGAGATAAACTGAAACTCACCTGCTTTGAATTGGCACCGAAAAGTGGGAATA
CCGGGGAGTTGAGATCTGTAATTCATAGTCACATCAAGGTCATCAAGACCAGGAAAAACA
AGAAAGACATACTCAATCCTGATTCAAGTATGGAAACTTCACCAGACTTTTTCTTCTAAA
ATCTGGATGTCATTGACGATAATGTTTATGGAGATAAGGTCTAAGTGCCTAAAAAAATGT
ACATATACCTGGTTGAAATACAACACTATACATACACACCACCATATATACTAGCTGTTA
ATCCTATGGAATGGGGTATTGGGAGTGCTTTTTTAATTTTTCATAGTTTTTTTTTAATAA
AATGGCATATTTTGCATCTACAACTTCTATAATTTGAAAAAATAAATAAACATTATCTTT
TTTGTGAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_005531 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAATAGGAGCAAG
CCAGCACTAGTCAGCTAACTAAGTGACTCAACCAAGGCCTTTTTTCCTTGTTATCTTTGC
AGATACTTCATTTTCTTAGCGTTTCTGGAGATTACAACATCCTGCGGTTCCGTTTCTGGG
AACTTTACTGATTTATCTCCCCCCTCACACAAATAAGCATTGATTCCTGCATTTCTGAAG
ATCTCAAGATCTGGACTACTGTTGAAAAAATTTCCAGTGAGGCTCACTTATGTCTGTAAA
GATGGGAAAAAAATACAAGAACATTGTTCTACTAAAAGGATTAGAGGTCATCAATGATTA
TCATTTTAGAATGGTTAAGTCCTTACTGAGCAACGATTTAAAACTTAATTTAAAAATGAG
AGAAGAGTATGACAAAATTCAGATTGCTGACTTGATGGAAGAAAAGTTCCGAGGTGATGC
TGGTTTGGGCAAACTAATAAAAATTTTCGAAGATATACCAACGCTTGAAGACCTGGCTGA
AACTCTTAAAAAAGAAAAGTTAAAAGTAAAAGGACCAGCCCTATCAAGAAAGAGGAAGAA
GGAAGTGGATGCTACTTCACCTGCACCCTCCACAAGCAGCACTGTCAAAACTGAAGGAGC
AGAGGCAACTCCTGGAGCTCAGAAAAGAAAAAAATCAACCANAGAAAAGGCTGCACCCAA
AGGGAGTAANGGTGTCCGAGAACAGACTCACCCTCCTCTCNCTGCAGAGCCCGCATGTCC
ACAGCCATGGGCCGTTNCCCATCTCCCAAGACCTCATTGTCAGCTTCACCCACACTTCTT
TCACTGAGAACCCGAAACAGTGGCCAATGTCAGTTACTCCCCAAAGAAATGTCTCAAAAC
CCCCATGATAGGGAAGTTCTGAT
>OriGene 3' read for NM_005531 unedited
CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAACAAAAAAGAAATGTTTA
TTTATTTTTTCAAATTATAGAAGTTGTAGATGCAAAATATGCCATTTTATTAAAAAAAAA
CTATGAAAAATTAAAAAAGCACTCCCAATACCCCATTCCATAGGATTAACAGCTAGTATA
TATGGTGGTGTGTATGTATAGTGTTGTATTTCAACCAGGTATATGTACATTTTTTTAGGC
ACTTAAACCTTATCTCCATAAACATTATCGTCAATGACATCCAGATTTTAGAAGAAAAAG
TCTGGTGAAGTTTCCATACTTGAATCAGGATTGAGTATGTCTTTCTTGTTTTTCCTGGAC
TTGATGACCTTGATGCGACTATGAATTACAGATCTCAACCGCCCGGTATTGCCACATTTC
GGTGCCCAACACAAACCTCGTTGACTCGCCGCTCGATCCCCTTCCTTCCCCTTCCACCGC
TCCACCCTGTAATCCCGACCCCCCGATCATTCTCCCCGATCCACCATCGCGCCACCACCA
CGCATCCTCACCCTTCATTGTATCTCCTTCATCTCCCCCCCGCCACTAACACGCCCCTCC
CCCCCATAATCCCTCCCTCTCTCCCACTTCCTCATTCGCGTTTACTTTCCCCCCGCATCC
TAACCCCGTCCTTATATCGCCCTCCCCAGTTGTCCTAATGCTACTCTCCGACCGCCCCCC
CATCCCGCTATCGCCTCCCCCCCCCGCGCATCTTTACGTTCCTCTCATGCCACTCTCCCG
CCTTTTACCGCCCTCCTACCTCCACCTACGTTTCTGTACTTATGTATATCCATCGCACTC
ATACTGCCCGTTCGCCGTACTTCCCCCTTCTCTTATAAGCCCGCTCCAGTTTATNTATCC
CCTCACTCCGTCTCCAATTTCCCCCATTTACCAATCCTCCATTACTACGATCGCACTCTA
CACGTCGTATGTCTGTTTCCTCCGNATCCTACCCCTATCTTGTTTACGTCTCTAGTCCTC
C
Restriction Sites NotI-NotI     
ACCN NM_005531
Insert Size 2770 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005531.1, NP_005522.1
RefSeq Size 2709 bp
RefSeq ORF 2190 bp
Locus ID 3428
Cytogenetics 1q23.1
Domains PAAD_DAPIN, HIN
Protein Families Transcription Factors
Gene Summary 'This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]'
Transcript Variant: This variant (2) consists of 11 exons and encodes isoform 2 (also known as isoform B, PMIDs:9718316 and 12588705). Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.

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