SEPP1 (SELENOP) (NM_005410) Human Untagged Clone

CAT#: SC116747

SEPP1 (untagged)-Human selenoprotein P, plasma, 1 (SEPP1), transcript variant 1 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: SELENOP
• Synonyms: SELP; SeP; SEPP; SEPP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116747 sequence for NM_005410 edited (data generated by NextGen Sequencing)
  ATGTGGAGAAGCCTGGGGCTTGCCCTGGCTCTCTGTCTCCTCCCATCGGGAGGAACAGAG
  AGCCAGGACCAAAGCTCCTTATGTAAGCAACCCCCAGCCTGGAGCATAAGAGATCAAGAT
  CCAATGCTAAACTCCAATGGTTCAGTGACTGTGGTTGCTCTTCTTCAAGCCAGCTGATAC
  CTGTGCATACTGCAGGCATCTAAATTAGAAGACCTGCGAGTAAAACTGAAGAAAGAAGGA
  TATTCTAATATTTCTTATATTGTTGTTAATCATCAAGGAATCTCTTCTCGATTAAAATAC
  ACACATCTTAAGAATAAGGTTTCAGAGCATATTCCTGTTTATCAACAAGAAGAAAACCAA
  ACAGATGTCTGGACTCTTTTAAATGGAAGCAAAGATGACTTCCTCATATATGATAGATGT
  GGCCGTCTTGTATATCATCTTGGTTTGCCTTTTTCCTTCCTAACTTTCCCATATGTAGAA
  GAAGCCATTAAGATTGCTTACTGTGAAAAGAAATGTGGAAACTGCTCTCTCACGACTCTC
  AAAGATGAAGACTTTTGTAAACGTGTATCTTTGGCTACTGTGGATAAAACAGTTGAAACT
  CCATCGCCTCATTACCATCATGAGCATCATCACAATCATGGACATCAGCACCTTGGCAGC
  AGTGAGCTTTCAGAGAATCAGCAACCAGGAGCACCAAATGCTCCTACTCATCCTGCTCCT
  CCAGGCCTTCATCACCACCATAAGCACAAGGGTCAGCATAGGCAGGGTCACCCAGAGAAC
  CGAGATATGCCAGCAAGTGAAGATTTACAAGATTTACAAAAGAAGCTCTGTCGAAAGAGA
  TGTATAAATCAATTACTCTGTAAATTGCCCACAGATTCAGAGTTGGCTCCTAGGAGCTGA
  TGCTGCCATTGTCGACATCTGATATTTGAAAAAACAGGGTCTGCAATCACCTGACAGTGT
  AAAGAAAACCTCCCATCTTTATGTAGCTGACAGGGACTTCGGGCAGAGGAGAACATAACT
  GAATCTTGTCAGTGACGTTTGCCTCCAGCTGCCTGACAAATAAGTCAGCAGCTTATACCC
  ACAGAAGCCAGTGCCAGTTGACGCTGAAAGAATCAGGCAAAAAAGTGAGAATGACCTTCA
  AACTAA
  
  Clone variation with respect to NM_005410.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005410 unedited
  GATCTTGGGATTTTGTAATCCGACTTACTATAGGCGGCCGCGCAATTCGCACGAGGCCAG
  AGTAAAGCAAANAGAAAGGAAGCAGGCCCGTTGGAAGTGGTTGTGACAACCCCAGCAATG
  TGGAGAAGCCTGGGGCTTGCCCTGGCTCTCTGTCTCCTCCCATCGGGAGGAACAGAGAGC
  CAGGACCAAAGCTCCTTATGTAAGCAACCCCCAGCCTGGAGCATAAGAGATCAAGATCCA
  ATGCTAAACTCCAATGGTTCAGTGACTGTGGTTGCTCTTCTTCAAGCCAGCTGATACCTG
  TGCATACTGCAGGCATCTAAATTAGAAGACCTGCGAGTAAAACTGAAGAAAGAAGGATAT
  TCTAATATTTCTTATATTGTTGTTAATCATCAAGGAATCTCTTCTCGATTAAAATACACA
  CATCTTAAGAATAAGGTTTCAGAGCATATTCCTGTTTATCAACAAGAAGAAAACCAAACA
  GATGTCTGGACTCTTTTAAATGGAAGCAAAGATGACTTCCTCATATATGATAGATGTGGC
  CGTCTTGTATATCATCTTGGTTTGCCTTTTTCCTTCCTAACTTTCCCATATGTAGAAGAA
  GCCATTAAGATTGCTTACTGTGAAAAGAAATGTGGAAACTGCTCTCTCACGACTCTCANA
  GATGAAGACTTTTGTAAACGTGTATCTTTGGCTACTGTGGATAAAACAGTTGANACTCCA
  TCGCCTCATTACCATCATGAGCATCATCACAATCATGGACATCAGCACCTTGGCAGCAGT
  GAGCTTTCAGAGAATCAGNCACCAGGAGCACCAAATGCTCCTACTCATCCTGCNTCTNCA
  GGCCTTCATCACCACCATAAGCACAAGGGTCAGCATAGGCAGGGNTCACCCAGAGAACCG
  AGATATGCCCCAGCAGTGAAGATTTACNNAGATTACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005410 unedited
  TAGCTATGAACCCGCGGCCGCAATCTAGGATCGAGNNNTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTAAAGGGTTTTATTGAATTTATTTGGACAAATCCGTACTGTATCC
  AATTCTGTACTGCATTCTTGCTTAAATAGTATTAACCATAAAGGAGGTCAGGTTTATAGG
  GTTTGGTTTACCTATTAAACCATCATTGACTATGGAAATACTTACTAAGCATTATAGAGA
  CAGACAATATTATCTTTCCCCTTATATCTTTTAAAACAGCCACTCAAGTTTTAAAAGAGT
  ATGATACAACATTAGAGAAAGAAAGATACAAAGGCTTTATTCATGTGTGATAGTAAAAAT
  CAGGATGAGTCTTAAATATACAAAAGATAAATGGATATTTAAAATAGTTATATATGCTTT
  TTTAACAAAATATTCACGTGTTAAGTATTTCTGGATCTTAAAATACAAAATCCACTTATT
  TTATTAGTTAAAAGCAAAAGATTGAAAAGACAAAGTAATATTGTTTTGAAAGATAAGTAA
  AGAAAAAAATGGAAAGCATGCCTTTGTTGTTCTTCCTCCCATTCTAAACTGCTAATTATC
  CAACAGAAACCCCTAGGTCATAGTTTACGTTTCTATTCTCATTAAAGCAAATACAACACT
  GGAAAAAGAAAAAAAAGACCTATTAACCAAAAGCTGCAATCACCTTTCAGTTGCTCCATC
  ATAAAAAATATGGTTTGAGTCAATATTTCTATGACATAAAATTTAAAATCTCGAACCCCA
  ATTAAGCAAATTTCTCCATGTTTGCACAAATCCTAANTTCTATTTTTGGCTCCACTAATT
  GGCGAGTTTAAAAAAACGCCTGGAAATGAAAATGTGTCTACACTAAATGGCGGAGATGTT
  CTATTTCAAAAATAAGGTTGGAAGCGCTTTCTAATTTTTTGGCTCGATCTTTCAGGGCAA
  ATGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005410
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005410.2, NP_005401.3
• RefSeq Size: 2164 bp
• Locus ID: 6414
• Cytogenetics: 5p12
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in human), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. The use of alternative polyadenylation sites, one located in between the two SECIS elements, results in two populations of mRNAs containing either both (predominant) or just the upstream SECIS element (PMID:27881738). Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Oct 2018]'
  Transcript Variant: This variant (1, also known as Sepp1a) represents the predominant transcript and encodes isoform 1. Variants 1 and 2 encode the same isoform.