SnoN (SKIL) (NM_005414) Human Untagged Clone

CAT#: SC116749

SKIL (untagged)-Human SKI-like oncogene (SKIL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SKIL
• Synonyms: SNO; SnoA; SnoI; SnoN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005414, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAAACCTCCAGACAAATTTCTCCTTGGTTCAGGGCTCAACTAAAAAACTGAATGGGATGGGAGATG
  ATGGCAGCCCCCCAGCGAAAAAAATGATAACGGACATTCATGCAAATGGAAAAACGATAAACAAGGTGCC
  AACAGTTAAGAAGGAACACTTGGATGACTATGGAGAAGCACCAGTGGAAACTGATGGAGAGCATGTTAAG
  CGAACCTGTACTTCTGTTCCTGAAACTTTGCATTTAAATCCCAGTTTGAAACACACATTGGCACAATTCC
  ATTTAAGTAGTCAGAGCTCGCTGGGTGGACCAGCAGCATTTTCTGCTCGGCATTCCCAAGAAAGCATGTC
  GCCTACTGTATTTCTGCCTCTTCCATCACCTCAGGTTCTTCCTGGCCCATTGCTCATCCCTTCAGATAGC
  TCCACAGAACTCACTCAGACTGTGTTGGAAGGGGAATCTATTTCTTGTTTTCAAGTTGGAGGAGAAAAGA
  GACTCTGTTTGCCCCAAGTCTTAAATTCTGTTCTCCGAGAATTTACACTCCAGCAAATAAATACAGTGTG
  TGATGAACTGTACATATATTGTTCAAGGTGTACTTCAGACCAGCTTCATATCTTAAAGGTACTGGGCATA
  CTTCCATTCAATGCCCCATCCTGTGGGCTGATTACATTAACTGATGCACAAAGATTATGTAATGCTTTAT
  TGCGGCCACGAACTTTTCCTCAAAATGGTAGCGTACTTCCTGCTAAAAGCTCATTGGCCCAGTTAAAGGA
  AACTGGCAGTGCCTTTGAAGTGGAGCATGAATGCCTAGGCAAATGTCAGGGTTTATTTGCACCCCAGTTT
  TATGTTCAGCCTGATGCTCCGTGTATTCAATGTCTGGAGTGTTGTGGAATGTTTGCACCCCAGACGTTTG
  TGATGCATTCTCACAGATCACCTGACAAAAGAACTTGCCACTGGGGCTTTGAATCAGCTAAATGGCATTG
  CTATCTTCATGTGAACCAAAAATACTTAGGAACACCTGAAGAAAAGAAACTGAAGATAATTTTAGAAGAA
  ATGAAGGAGAAGTTTAGCATGAGAAGTGGAAAGAGAAATCAATCCAAGACAGATGCACCATCAGGAATGG
  AATTACAGTCATGGTATCCTGTTATAAAGCAGGAAGGTGACCATGTTTCTCAGACACATTCATTTTTACA
  CCCCAGCTACTACTTATACATGTGTGATAAAGTGGTTGCCCCAAATGTGTCACTTACTTCTGCTGTATCC
  CAGTCTAAAGAGCTCACAAAGACAGAGGCAAGTAAGTCCATATCAAGACAGTCAGAGAAGGCTCACAGTA
  GTGGTAAACTTCAAAAAACAGTGTCTTATCCAGATGTCTCACTTGAGGAACAGGAGAAAATGGATTTAAA
  AACAAGTAGAGAATTATGTAGCCGTTTAGATGCATCAATCTCAAATAATTCTACAAGTAAAAGGAAATCT
  GAGTCTGCCACTTGCAACTTAGTCAGAGACATAAACAAAGTGGGAATTGGCCTTGTTGCTGCCGCTTCAT
  CTCCGCTTCTTGTGAAAGATGTCATTTGTGAGGATGATAAGGGAAAAATCATGGAAGAAGTAATGAGAAC
  TTATTTAAAACAACAGGAAAAACTAAACTTGATTTTGCAAAAGAAGCAACAACTTCAGATGGAAGTAAAA
  ATGTTGAGTAGTTCAAAATCTATGAAGGAACTCACTGAAGAACAGCAGAATTTACAGAAAGAGCTTGAAT
  CTTTGCAGAATGAACATGCTCAAAGAATGGAAGAATTTTATGTTGAACAGAAAGACTTAGAGAAAAAATT
  GGAGCAGATAATGAAGCAAAAATGTACCTGTGACTCAAATTTAGAAAAAGACAAAGAGGCTGAATATGCA
  GGACAGTTGGCAGAACTGAGGCAGAGATTGGACCATGCTGAGGCCGATAGGCAAGAACTCCAAGATGAAC
  TCAGACAGGAACGGGAAGCAAGACAGAAGTTAGAGATGATGATAAAAGAGCTAAAGCTGCAAATTCTGAA
  ATCATCAAAGACTGCTAAAGAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005414 unedited
  NGTTCAAAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGTGAGACAGAA
  AATGCTTGAACCCGGGAGGCGGAGGTTGCAGTATGCCGAGATCGCTGCACTTCAGCCTGG
  GCGACAGAGCGAGACTCCGTAAAAAAAAGAAAAAAAAAAAAAATAAAAGAAACTGGTTTC
  AAATTGGCCCTTTGGCCTCTGGAGCAAATTCAAATGTAACTCTTCCCCAACCCCCCTTCT
  CTTCTTCCAGATTAATTAAAAGAAGAATGAACTATAATCCTTGAAGATAACTGGGCAATT
  TTTTAAGTCGGAGGCTGTTCTTACTGGTGTGAGGATTTACACACGTCTTCAGTTTTTCAG
  CACAGACCAGCAGACCATCATTTTTAGAGGAAATACTCCCTCTGCCCTCCTTTTTGGTTT
  CCTTGGTGGTAAAGATTAAATTTGGTTGCATCATTTTGACTTGTGTTTGAGTCTAGGTTT
  TATGGCACAAGGAATGGCATAAACTTTTCATGTGTTTTGGTTAAAACAAACCAGACCATT
  GCATTGACCCTGGACATCTTTAATTGAGAAATTGGTAACTTTATTTTAATATGTATATCT
  GAAGAATTCAAGAAAACAAAGGCATCCTCAGAGGTGTGCCTCTTTTCTTTATTATTAGAG
  GCAAAACGAACAATTTTATAGGATTTGTAGTGAAATTATACCAGATTATAAGGAGAACCA
  AAACTAAGTCGCAAAATTTATTAATTTAAGGGGCTCTCGCTTTGAAAGTTTGAGAGTAAG
  TTACGATAGGCATTTGTATCCATTCATTACTTTCCTCTTTTCAAATAGCACTAAATAGAA
  TGCTAATCTCAGACTTAAATATTACAGNAAGGTGTACATGGGAAAACCNTCAGACAATTC
  TNCTTGGNTCAGGNCTCACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005414 unedited
  GTACCGCGGCACGCAATCTAGTGTCGAGNTNTTTTTTTTTTTTTTTTTTTTTTTCAAAAA
  TTATCACATTTTATTTTTGAAAATTGCTGCTTAAAAAAAAATCTAACTATAACCACATTT
  AAACTGAAAAACAAATTACAACTGCTGCTTCTGTTTAGCAGCAGCACATAACAACACATT
  CTTCTTCCCCACTGGGCTCATGGGGTATGCTATAACCTCAGTAAAATTCCTCCTAGGGTA
  CACATTAAAAAGAAAAGAACTGGGGGAAATTCATTCCACAATCTGAACAAAGTAAAAGTA
  AATTTGGGGTATGTTAATGAGTACACCATATCAACTTAAAAAAATTGGGTTAATGAGAAA
  AGGGGAGATTCAACTCCTTGAGTAGCTCTATATACTTAAAATATAAAGCCACCATTGTAT
  CTAAAATTGAGTCTGAAATATGGATAACAAAATTGACCACAGCTATTTTCCATCACATAT
  TGCAGCTTTTAAAAAGTACACAATTGTTAAGTTTGCATTAAAAATATAAGCAGATTACCT
  ACTAGTTGAAACCATATAGTATTGTTACTAAGCTGATTTTTAAAAAAGTACAAAGAAACA
  TACATTTTCATTCATTTGGAAAATGTAAAAATTTAAGCACTAATATATAATCTCTTCACT
  TTCTCTGATCTACAAATGGATAGAATGCCTAGTTATCGTCATGCAGATAAATTCTTCAGA
  ATTCAATTACCAAAGCAAGCAACAAACAAAAAAAACCTTGTCAGTAATACACAGATGAAT
  CTCTTTAACAGTTTCTATTCTTTAGCAGTCTTTGATGATTTCAGAAATTGCAGCTTTAGC
  TCTTTAATATCATCTCTAACTTCTGGCTGGCTTCCCGTCCTGGCTGAGTTATCTGGGAGT
  TCTGGCTATCGGCCTCACATGGTCCATCTTGGCCAAGTCTGCCACTGCCTGCTATTAGCC
  CCTTGTCTTTTTTAATTGAGCCAGGNACATTTTGTTATTATCGCCCCATTTTTCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005414
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005414.2, NP_005405.1
• RefSeq Size: 3111 bp
• RefSeq ORF: 2055 bp
• Locus ID: 6498
• Cytogenetics: 3q26.2
• Domains: Ski_Sno
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (1) encodes the longest isoform (1, also known as snoN). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.