HINT1 (NM_005340) Human Untagged Clone

CAT#: SC116762

HINT1 (untagged)-Human histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HINT1
• Synonyms: HINT; NMAN; PKCI-1; PRKCNH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116762 sequence for NM_005340 edited (data generated by NextGen Sequencing)
  ATGGCAGATGAGATTGCCAAGGCTCAGGTCGCTCGGCCTGGTGGCGACACGATCTTTGGG
  AAGATCATCCGCAAGGAAATACCAGCCAAAATCATTTTTGAGGATGACCGGTGCCTTGCT
  TTCCATGACATTTCCCCTCAAGCACCAACACATTTTCTGGTGATACCCAAGAAACATATA
  TCCCAGATTTCTGTGGCAGAAGATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTT
  GGCAAGAAATGTGCTGCTGATCTGGGCCTGAATAAGGGTTATCGAATGGTGGTGAATGAA
  GGTTCAGATGGTGGACAGTCTGTCTATCACGTTCATCTCCATGTTCTTGGAGGTCGGCAA
  ATGCATTGGCCTCCTGGTTAA
  
  Clone variation with respect to NM_005340.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005340 unedited
  NGTAACGTACAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCCT
  CGTGCCGAATTCGGCACGAGGCCGAGCCTCTCCTCTGGCCGCCGCGCGGGAGAGAGGCCG
  AGATGGCAGATGAGATTGCCAAGGCTCAGGTCGCTCGGCCTGGTGGCGACACGATCTTTG
  GGAAGATCATCCGCAAGGAAATACCAGCCAAAATCATTTTTGAGGATGACCGGTGCCTTG
  CTTTCCATGACATTTCCCCTCAAGCACCAACACATTTTCTGGTGATACCCAAGAAACATA
  TATCCCAGATTTCTGTGGCAGAAGATGATGATGAAAGTCTTCTTGGACACTTAATGATTG
  TTGGCAAGAAATGTGCTGCTGATCTGGGCCTGAATAAGGGTTATCGAATGGTGGTGAATG
  AAGGTTCAGATGGTGGACAGTCTGTCTATCACGTTCATCTCCATGTTCTTGGAGGTCGGC
  AAATGCATTGGCCTCCTGGTTAAGCACGTTTTGGGGATAATTTTCTCTTCTTTAGGCAAT
  GATTAAGTTAGGCAATTTCCAGTATGTTAAGTAACACACTTATTTTTGCCTGTGTATGGA
  GAGATTCAAGAAATAATTTTAAAACCGCATACATAATAAAAGACATTGTTGCATGGCTTA
  TAGTCTCTCTGAGTGTTGCGTTTGATTTGTTATTTTAAAAACATATTTGTTGGAATATGT
  GAAGGGTGGGTTTATAATTTTGGGATGGTTTCTTGCTTTTTCTTCCCAAGGAGGACTCAA
  CATATGAACATCGCATTGTTTTAATGGTGTTACTAGTCTTTGAAGACAAATAATGAGAGT
  TTATAAATTTAGCTTTAACTTCGAAATATCTTTCCAGCCTAAAATAGGTCCCACTTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005340
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005340.3, NP_005331.1
• RefSeq Size: 675 bp
• RefSeq ORF: 381 bp
• Locus ID: 3094
• Cytogenetics: 5q23.3
• Domains: HIT
• Gene Summary: 'This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) encodes the functional protein.