HSP70-1A (HSPA1A) (NM_005345) Human Untagged Clone

CAT#: SC116766

HSPA1A (untagged)-Human heat shock 70kDa protein 1A (HSPA1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPA1A
• Synonyms: HEL-S-103; HSP70-1; HSP70-1A; HSP70-2; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116766 sequence for NM_005345 edited (data generated by NextGen Sequencing)
  ATGGCCAAAGCCGCGGCGATCGGCATCGACCTGGGCACCACCTACTCCTGCGTGGGGGTG
  TTCCAACACGGCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACCACCCCCAGC
  TACGTGGCCTTCACGGACACCGAGCGGCTCATCGGGGATGCGGCCAAGAACCAGGTGGCG
  CTGAACCCGCAGAACACCGTGTTTGACGCGAAGCGGCTGATCGGCCGCAAGTTCGGCGAC
  CCGGTGGTGCAGTCGGACATGAAGCACTGGCCTTTCCAGGTGATCAACGACGGAGACAAG
  CCCAAGGTGCAGGTGAGCTACAAGGGGGACACCAAGGCATTCTACCCCGAGGAGATCTCG
  TCCATGGTGCTGACCAAGATGAAGGAGATCGCCGAGGCGTACCTGGGCTACCCGGTGACC
  AACGCGGTGATCACCGTGCCGGCCTACTTCAACGACTCGCAGCGCCAGGCCACCAAGGAT
  GCGGGTGTGATCGCGGGGCTCAACGTGCTGCGGATCATCAACGAGCCCACGGCCGCCGCC
  ATCGCCTACGGCCTGGACAGAACGGGCAAGGGGGAGCGCAACGTGCTCATCTTTGACCTG
  GGCGGGGGCACCTTCGACGTGTCCATCCTGACGATCGACGACGGCATCTTCGAGGTGAAG
  GCCACGGCCGGGGACACCCACCTGGGTGGGGAGGACTTTGACAACAGGCTGGTGAACCAC
  TTCGTGGAGGAGTTCAAGAGAAAACACAAGAAGGACATCAGCCAGAACAAGCGAGCCGTG
  AGGCGGCTGCGCACCGCCTGCGAGAGGGCCAAGAGGACCCTGTCGTCCAGCACCCAGGCC
  AGCCTGGAGATCGACTCCCTGTTTGAGGGCATCGACTTCTACACGTCCATCACCAGGGCG
  AGGTTCGAGGAGCTGTGCTCCGACCTGTTCCGAAGCACCCTGGAGCCCGTGGAGAAGGCT
  CTGCGCGACGCCAAGCTGGACAAGGCCCAGATTCACGACCTGGTCCTGGTCGGGGGCTCC
  ACCCGCATCCCCAAGGTGCAGAAGCTGCTGCAGGACTTCTTCAACGGGCGCGACCTGAAC
  AAGAGCATCAACCCCGACGAGGCTGTGGCCTACGGGGCGGCGGTGCAGGCGGCCATCCTG
  ATGGGGGACAAGTCCGAGAACGTGCAGGACCTGCTGCTGCTGGACGTGGCTCCCCTGTCG
  CTGGGGCTGGAGACGGCCGGAGGCGTGATGACTGCCCTGATCAAGCGCAACTCCACCATC
  CCCACCAAGCAGACGCAGATCTTCACCACCTACTCCGACAACCAACCCGGGGTGCTGATC
  CAGGTGTACGAGGGCGAGAGGGCCATGACGAAAGACAACAATCTGTTGGGGCGCTTCGAG
  CTGAGCGGCATCCCTCCGGCCCCCAGGGGCGTGCCCCAGATCGAGGTGACCTTCGACATC
  GATGCCAACGGCATCCTGAACGTCACGGCCACGGACAAGAGCACCGGCAAGGCCAACAAG
  ATCACCATCACCAACGACAAGGGCCGCCTGAGCAAGGAGGAGATCGAGCGCATGGTGCAG
  GAGGCGGAGAAGTACAAAGCGGAGGACGAGGTGCAGCGCGAGAGGGTGTCAGCCAAGAAC
  GCCCTGGAGTCCTACGCCTTCAACATGAAGAGCGCCGTGGAGGATGAGGGGCTCAAGGGC
  AAGATCAGCGAGGCSGACAAGAAGAAGGTGCTGGACAAGTGTCAAGAGGTCATCTCGTGG
  CTGGACGCCAACACCTTGGCCGAGAAGGACGAGTTTGAGCACAAGAGGAAGGAGCTGGAG
  CAGGTGTGTAACCCCATCATCAGCGGACTGTACCAGGGTGCCGGTGGTCCCGGGCCTGGG
  GGCTTCGGGGCTCAGGGTCCCAAGGGAGGGTCTGGGTCAGGCCCCACCATTGAGGAGGTA
  GATTAG
  
  Clone variation with respect to NM_005345.5
  222 t=>c;330 g=>c;1695 g=>s
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005345 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCGACAGTCC
  ACTACCTTTTTCGAGAGTGACTCCCGTTGTCCCAAGGCTTCCCAGAGCGAACCTGTGCGG
  CTGCAGGCACCGGCGCGTCGAGTTTCCGGCGTCCGGAAGGACCGAGCTCTTCTCGCGGAT
  CCAGTGTTCCGTTTCCAGCCCCCAATCTCAGAGCCGAGCCGACAGAGAGCAGGGAACCGG
  CATGGCCAAAGCCGCGGCGATCGGCATCGACCTGGGCACCACCTACTCCTGCGTGGGGGT
  GTTCCAACACGGCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACCACCCCCAG
  CTACGTGGCCTTCACGGACACCGAGCGGCTCATCGGGGATGCGGCCAAGAACCAGGTGGC
  GCTGAACCCGCAGAACACCGTGTTTGACGCGAAGCGGCTGATCGGCCGCAAGTTCGGCGA
  CCCGGTGGTGCAGTCGGACATGAAGCACTGGCCTTTCCAGGTGATCAACGACGGAGACAA
  GCCCAAGGTGCAGGTGAGCTACAAGGGGGACACCAAGGCATTCTACCCCGAGGAGATCTC
  GTCCATGGTGCTGACCAAGATGAAGGAGATCGCCGAGGCGTACCTGGGCTACCCGGTGAC
  AACGCGGTGATCACCGTGCCGGCCTACTTCAACGACTCGCAGCGCCAGGCCACCAAGGAT
  GCNGGNNTGTGATCGCGGNGCTCAAACGTGCTGCGGATCATCAACGAGCCCACGGNCGNC
  GCCATTCGCTACGGNCTGNACAGAACGGGCAGGGGGGAGCGCACGTGCTCATCTTTGACC
  CTGGCGGGGCACCTTNCGACGTGTCCTTCTTGACGATCGACGACGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005345 unedited
  GCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTGAATTTTAAAGTTTATTTACTTTGAAGTAACCAACTTAAAAATGGCCTGAGTTA
  AGTGTATTAAAAAGAAGAAATAGTCGTAAGATGGCAGTATAAATTCATCTCTGCATGTAG
  AAACCGGAAAAAAGCAAGTTCAGTACTTCACCAAAAAATTTCAACATTGCAAACACAGGA
  AATTGAGAACTGACAAACAGAAATACTAGGAAATGCAAAGTCTTGAAGCTCCAAAACAAA
  AACAGCAATCTTGGAAAGGCCCCTAATCTACCTCCTCAATGGGGGGGCCTGACCCAGACC
  CTCCCTTGGGACCCTGAGCCCCGAAGCCCCCAGGCCCGGGACCACCGGCACCCTGGTACA
  GTCCGCTGATGATGGGGTTACACACCTGCTCCAGCTCCTTCCTTTTGTGCTCAAACTCGT
  CCTTCTCGGCCAAGGTGTTGGCGTCCAGCCACGAGATGACCTCTTGACACTTGTTCAGAA
  CCTTNTTNTTGTCCGCCTCGCTGATCTTGCCCTTGAGCCCCTTATCCTTCACGGCGCTCT
  TCATGTTGAAGGCGTAAGACTCCAGGGCGTTCTTGGTTGACACCCTCTCGCGCTGCACCT
  CGGCCTCCGCTTTGTACTTCTCCGCCTACTGTACCATGCGCTCGATCTCCTCCTTGCTTA
  GGCGGCCCTTGTNGTTTGTGATGGAGATCTTGTTGGCCTTGCCGGGCCTTTGCCCTGGCC
  GTGACGTTAAAGATGCCGTTGGCATCGATGTGCAAAGTCACCTGAACCTGGGCCCGCCCC
  TGGGGCCCGTAGGATGCCGCTCATTTCGATTCGCCCCACGATTGTTGTCTTCGCATGGCC
  TTTTGCCCTTGTAACCTGGATAGCCCCCGGTTGTTGTCGAGAAGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005345
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005345.4, NP_005336.2
• RefSeq Size: 2383 bp
• RefSeq ORF: 1926 bp
• Locus ID: 3303
• Cytogenetics: 6p21.33
• Domains: HSP70
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Prion diseases, Spliceosome
• Gene Summary: 'This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]'