SMAD4 (NM_005359) Human Untagged Clone

CAT#: SC116771

SMAD4 (untagged)-Human SMAD family member 4 (SMAD4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMAD4
• Synonyms: DPC4; JIP; MADH4; MYHRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116771 sequence for NM_005359 edited (data generated by NextGen Sequencing)
  ATGGACAATATGTCTATTACGAATACACCAACAAGTAATGATGCCTGTCTGAGCATTGTG
  CATAGTTTGATGTGCCATAGACAAGGTGGAGAGAGTGAAACATTTGCAAAAAGAGCAATT
  GAAAGTTTGGTAAAGAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCT
  ATAACTACAAATGGAGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGG
  AGGCTTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTCTGGAGG
  TGGCCTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTGTCAGTATGCGTTTGAC
  TTAAAATGTGATAGTGTCTGTGTGAATCCATATCACTACGAACGAGTTGTATCACCTGGA
  ATTGATCTCTCAGGATTAACACTGCAGAGTAATGCTCCATCAAGTATGATGGTGAAGGAT
  GAATATGTGCATGACTTTGAGGGACAGCCATCGTTGTCCACTGAAGGACATTCAATTCAA
  ACCATCCAGCATCCACCAAGTAATCGTGCATCGACAGAGACATACAGCACCCCAGCTCTG
  TTAGCCCCATCTGAGTCTAATGCTACCAGCACTGCCAACTTTCCCAACATTCCTGTGGCT
  TCCACAAGTCAGCCTGCCAGTATACTGGGGGGCAGCCATAGTGAAGGACTGTTGCAGATA
  GCATCAGGGCCTCAGCCAGGACAGCAGCAGAATGGATTTACTGGTCAGCCAGCTACTTAC
  CATCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTG
  CCTCACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCCGGACAT
  TACTGGCCTGTTCACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCT
  GAGTATTGGTGTTCCATTGCTTACTTTGAAATGGATGTTCAGGTAGGAGAGACATTTAAG
  GTTCCTTCAAGCTGCCCTATTGTTACTGTTGATGGATACGTGGACCCTTCTGGAGGAGAT
  CGCTTTTGTTTGGGTCAACTCTCCAATGTCCACAGGACAGAAGCCATTGAGAGAGCAAGG
  TTGCACATAGGCAAAGGTGTGCAGTTGGAATGTAAAGGTGAAGGTGATGTTTGGGTCAGG
  TGCCTTAGTGACCACGCGGTCTTTGTACAGAGTTACTACTTAGACAGAGAAGCTGGGCGT
  GCACCTGGAGATGCTGTTCATAAGATCTACCCAAGTGCATATATAAAGGTCTTTGATTTG
  CGTCAGTGTCATCGACAGATGCAGCAGCAGGCGGCTACTGCACAAGCTGCAGCAGCTGCC
  CAGGCAGCAGCCGTGGCAGGAAACATCCCTGGCCCAGGATCAGTAGGTGGAATAGCTCCA
  GCTATCAGTCTGTCAGCTGCTGCTGGAATTGGTGTTGATGACCTTCGTCGCTTATGCATA
  CTCAGGATGAGTTTTGTGAAAGGCTGGGGACCGGATTACCCAAGACAGAGCATCAAAGAA
  ACACCTTGCTGGATTGAAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTT
  CATACCATGCCGATTGCAGACCCACAACCTTTAGACTGA
  
  Clone variation with respect to NM_005359.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005359 unedited
  GTTCGCATTTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGGCTCTCCGGGCCGCCGGGNGAAAGCTACGGGCCCGGTGCGTCCGCG
  GACCAGCAGCGCGGGAGAGCGGACTCCCCTCGCCACCGCCCGAGCCCAGGTTATCCTGAA
  TACATGTCTAACAATTTTCCTTGCAACGTTAGCTGTTGTTTTTCACTGTTTCCAAAGGAT
  CAAAATTGCTTCAGAAATTGGAGACATATTTGATTTAAAAGGAAAAACTTGAACAAATGG
  ACAATATGTCTATTACGAATACACCAACAAGTAATGATGCCTGTCTGAGCATTGTGCATA
  GTTTGATGTGCCATAGACAAGGTGGAGAGAGTGAAACATTTGCAAAAAGAGCAATTGAAA
  GTTTGGTAAAGAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAA
  CTACAAATGGAGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGGAGGC
  TTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTCTGGAGGTGGC
  CTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTGTCAGTATGCGTTTGACTTAN
  AATGTGATAGTGTCTGTGTGAATCCATATCACTACGAACGAGTTGTATCACCTGGAANTG
  ATCTCTCAGGATTAACACTGCAGAGTAATGCTCCCATCAGTATGATGGTGAAGGATGAAT
  ATGTGCATGACTTTGAGGGACAGCCCATCGTTGTCCACTGAGGGACATCANTNCANACCT
  CCAGCATCCACCCAGTATCGTGCATCGACAGAGACATACAGCACCCCAGCTCTGTTAGCC
  CCATCTGAGTCTAATGCCTACAGCACTGGCCACTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005359 unedited
  ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTGAGATTCAG
  TTTTATTTATGCTACCAGCTAGGTAAAAAGCATACATATTGTGTCTGTGGTACAGTCAAT
  ACATCTGCAACTTTTCTACTTAAATTCTCTATACAAAGTCACTGCCAATTGATATGATCA
  TTGATGGCAAAGGGCTTAGAATAACCAAAAGATATAAAAAAGTTTGCAGTCTTGCCCCAA
  GATACAAAAACTGAATTTTAAACAATATCATAACATACATGATTTAAACATTATACCAAA
  AAAAGTCACATCAAATCAAGTACAAAAATATCCAAACTACCTATTATGAACTGTAATGTT
  TCCATTATTCTGCACAGTATTTAACATAGAATTTAGCAGTTTCCAAAATATTCAGTTTAT
  TCATTATTTAGTTTAAGCATTGCCACCTTTGCAGAACAGTGAGACATTAGGTAGAGTGAC
  GCTGCTTTTAGTGTTTTAAAAAATACAAAACCTTTCCCTATGAATGAAGGAAAAGAAATT
  CCTCTTATAGGCTTCTTAGCAACCAACCTTGTGCCTAGAAATACAGAAGAATACAACTAC
  TGTTTAACAGGGGAAAAGTATACTTCATCAACAGTCATCCACTAGGAATAACAAACGTAA
  TTTTAAATTNTTTTGTCTGGTAATTNTAAGGGATTCTCAATATACACACAGATTAAGAGT
  TTTTTGCCTTGGNCAAATAAAACTGAAGTTGCAAGACAATCTGAATTGAAACCATCTTCA
  CTAATATAACTTGCAAAAAATATTTTGATGAAAGATTACATACATTATTAACTTACTTTT
  TAAACACAGAGTAAAGTTACTCTTTGGTAAAATNAACTNACCCACATGGCACATTATTTT
  TGAAGTATGNTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005359
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005359.3, NP_005350.1
• RefSeq Size: 3220 bp
• RefSeq ORF: 1659 bp
• Locus ID: 4089
• Cytogenetics: 18q21.2
• Domains: DWB, DWA, MH1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]'