SMAD4 (NM_005359) Human Untagged Clone

CAT#: SC116771

SMAD4 (untagged)-Human SMAD family member 4 (SMAD4)


  "NM_005359" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SMAD4
Synonyms DPC4; JIP; MADH4; MYHRS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116771 sequence for NM_005359 edited (data generated by NextGen Sequencing)
ATGGACAATATGTCTATTACGAATACACCAACAAGTAATGATGCCTGTCTGAGCATTGTG
CATAGTTTGATGTGCCATAGACAAGGTGGAGAGAGTGAAACATTTGCAAAAAGAGCAATT
GAAAGTTTGGTAAAGAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCT
ATAACTACAAATGGAGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGG
AGGCTTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTCTGGAGG
TGGCCTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTGTCAGTATGCGTTTGAC
TTAAAATGTGATAGTGTCTGTGTGAATCCATATCACTACGAACGAGTTGTATCACCTGGA
ATTGATCTCTCAGGATTAACACTGCAGAGTAATGCTCCATCAAGTATGATGGTGAAGGAT
GAATATGTGCATGACTTTGAGGGACAGCCATCGTTGTCCACTGAAGGACATTCAATTCAA
ACCATCCAGCATCCACCAAGTAATCGTGCATCGACAGAGACATACAGCACCCCAGCTCTG
TTAGCCCCATCTGAGTCTAATGCTACCAGCACTGCCAACTTTCCCAACATTCCTGTGGCT
TCCACAAGTCAGCCTGCCAGTATACTGGGGGGCAGCCATAGTGAAGGACTGTTGCAGATA
GCATCAGGGCCTCAGCCAGGACAGCAGCAGAATGGATTTACTGGTCAGCCAGCTACTTAC
CATCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTG
CCTCACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCCGGACAT
TACTGGCCTGTTCACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCT
GAGTATTGGTGTTCCATTGCTTACTTTGAAATGGATGTTCAGGTAGGAGAGACATTTAAG
GTTCCTTCAAGCTGCCCTATTGTTACTGTTGATGGATACGTGGACCCTTCTGGAGGAGAT
CGCTTTTGTTTGGGTCAACTCTCCAATGTCCACAGGACAGAAGCCATTGAGAGAGCAAGG
TTGCACATAGGCAAAGGTGTGCAGTTGGAATGTAAAGGTGAAGGTGATGTTTGGGTCAGG
TGCCTTAGTGACCACGCGGTCTTTGTACAGAGTTACTACTTAGACAGAGAAGCTGGGCGT
GCACCTGGAGATGCTGTTCATAAGATCTACCCAAGTGCATATATAAAGGTCTTTGATTTG
CGTCAGTGTCATCGACAGATGCAGCAGCAGGCGGCTACTGCACAAGCTGCAGCAGCTGCC
CAGGCAGCAGCCGTGGCAGGAAACATCCCTGGCCCAGGATCAGTAGGTGGAATAGCTCCA
GCTATCAGTCTGTCAGCTGCTGCTGGAATTGGTGTTGATGACCTTCGTCGCTTATGCATA
CTCAGGATGAGTTTTGTGAAAGGCTGGGGACCGGATTACCCAAGACAGAGCATCAAAGAA
ACACCTTGCTGGATTGAAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTT
CATACCATGCCGATTGCAGACCCACAACCTTTAGACTGA

Clone variation with respect to NM_005359.5
>OriGene 5' read for NM_005359 unedited
GTTCGCATTTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACGAGGCCTCGTGCCG
AATTCGGCACGAGGGCTCTCCGGGCCGCCGGGNGAAAGCTACGGGCCCGGTGCGTCCGCG
GACCAGCAGCGCGGGAGAGCGGACTCCCCTCGCCACCGCCCGAGCCCAGGTTATCCTGAA
TACATGTCTAACAATTTTCCTTGCAACGTTAGCTGTTGTTTTTCACTGTTTCCAAAGGAT
CAAAATTGCTTCAGAAATTGGAGACATATTTGATTTAAAAGGAAAAACTTGAACAAATGG
ACAATATGTCTATTACGAATACACCAACAAGTAATGATGCCTGTCTGAGCATTGTGCATA
GTTTGATGTGCCATAGACAAGGTGGAGAGAGTGAAACATTTGCAAAAAGAGCAATTGAAA
GTTTGGTAAAGAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAA
CTACAAATGGAGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGGAGGC
TTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTCTGGAGGTGGC
CTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTGTCAGTATGCGTTTGACTTAN
AATGTGATAGTGTCTGTGTGAATCCATATCACTACGAACGAGTTGTATCACCTGGAANTG
ATCTCTCAGGATTAACACTGCAGAGTAATGCTCCCATCAGTATGATGGTGAAGGATGAAT
ATGTGCATGACTTTGAGGGACAGCCCATCGTTGTCCACTGAGGGACATCANTNCANACCT
CCAGCATCCACCCAGTATCGTGCATCGACAGAGACATACAGCACCCCAGCTCTGTTAGCC
CCATCTGAGTCTAATGCCTACAGCACTGGCCACTTT
>OriGene 3' read for NM_005359 unedited
ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTGAGATTCAG
TTTTATTTATGCTACCAGCTAGGTAAAAAGCATACATATTGTGTCTGTGGTACAGTCAAT
ACATCTGCAACTTTTCTACTTAAATTCTCTATACAAAGTCACTGCCAATTGATATGATCA
TTGATGGCAAAGGGCTTAGAATAACCAAAAGATATAAAAAAGTTTGCAGTCTTGCCCCAA
GATACAAAAACTGAATTTTAAACAATATCATAACATACATGATTTAAACATTATACCAAA
AAAAGTCACATCAAATCAAGTACAAAAATATCCAAACTACCTATTATGAACTGTAATGTT
TCCATTATTCTGCACAGTATTTAACATAGAATTTAGCAGTTTCCAAAATATTCAGTTTAT
TCATTATTTAGTTTAAGCATTGCCACCTTTGCAGAACAGTGAGACATTAGGTAGAGTGAC
GCTGCTTTTAGTGTTTTAAAAAATACAAAACCTTTCCCTATGAATGAAGGAAAAGAAATT
CCTCTTATAGGCTTCTTAGCAACCAACCTTGTGCCTAGAAATACAGAAGAATACAACTAC
TGTTTAACAGGGGAAAAGTATACTTCATCAACAGTCATCCACTAGGAATAACAAACGTAA
TTTTAAATTNTTTTGTCTGGTAATTNTAAGGGATTCTCAATATACACACAGATTAAGAGT
TTTTTGCCTTGGNCAAATAAAACTGAAGTTGCAAGACAATCTGAATTGAAACCATCTTCA
CTAATATAACTTGCAAAAAATATTTTGATGAAAGATTACATACATTATTAACTTACTTTT
TAAACACAGAGTAAAGTTACTCTTTGGTAAAATNAACTNACCCACATGGCACATTATTTT
TGAAGTATGNTC
Restriction Sites NotI-NotI     
ACCN NM_005359
Insert Size 3570 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005359.3, NP_005350.1
RefSeq Size 3220 bp
RefSeq ORF 1659 bp
Locus ID 4089
Cytogenetics 18q21.2
Domains DWB, DWA, MH1
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
Gene Summary 'This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]'

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