Myoglobin (MB) (NM_005368) Human Untagged Clone

CAT#: SC116773

MB (untagged)-Human myoglobin (MB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MB
• Synonyms: myoglobgin; PVALB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116773 sequence for NM_005368 edited (data generated by NextGen Sequencing)
  ATGGGGCTCAGCGACGGGGAATGGCAGTTGGTGCTGAACGTCTGGGGGAAGGTGGAGGCT
  GACATCCCAGGCCATGGGCAGGAAGTCCTCATCAGGCTCTTTAAGGGTCACCCAGAGACT
  CTGGAGAAGTTTGACAAGTTCAAGCACCTGAAGTCAGAGGACGAGATGAAGGCATCTGAG
  GACTTAAAGAAGCATGGTGCCACTGTGCTCACCGCCCTGGGTGGCATCCTTAAGAAGAAG
  GGGCATCATGAGGCAGAGATTAAGCCCCTGGCACAGTCGCATGCCACCAAGCACAAGATC
  CCCGTGAAGTACCTGGAGTTCATCTCGGAATGCATCATCCAGGTTCTGCAGAGCAAGCAT
  CCCGGGGACTTTGGTGCTGATGCCCAGGGGGCCATGAACAAGGCCCTGGAGCTGTTCCGG
  AAGGACATGGCCTCCAACTACAAGGAGCTGGGCTTCCAGGGCTAG
  
  Clone variation with respect to NM_005368.2
  174 g=>a;204 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005368 unedited
  NTTGTGCAAATATTTGTATACGACTCACTATAGGGCGGCCCGCGATTCGGCACCAGAGAC
  TCTGAAGCCAGACTACCTGGGTCCCAATCTTGGGCTTGGTATTTCCTCGCTGTGTGACTC
  TGGACTGCGCCATGGGGCTCAGCGACGGGGAATGGCAGTTGGTGCTGAACGTCTGGGGGA
  AGGTGGAGGCTGACATCCCAGGCCATGGGCAGGAAGTCCTCATCAGGCTCTTTAAGGGTC
  ACCCAGAGACTCTGGAGAAGTTTGACAAGTTCAAGCACCTGAAGTCAGAGGACGAGATGA
  AGGCATCTGAGGACTTAAAGAAGCATGGTGCCACTGTGCTCACCGCCCTGGGTGGCATCC
  TTAAGAAGAAGGGGCATCATGAGGCAGAGATTAAGCCCCTGGCACAGTCGCATGCCACCA
  AGCACAAGATCCCCGTGAAGTACCTGGAGTTCATCTCGGAATGCATCATCCAGGTTCTGC
  AGAGCAAGCATCCCGGGGACTTTGGTGCTGATGCCCAGGGGGCCATGAACAAGGCCCTGG
  AGCTGTTCCGGAAGGACATGGCCTCCAACTACAAGGAGCTGGGCTTCCAGGGCTAGGCCC
  CTGCCGCTCCCACCCCCACCCATCTGGGCCCCGGGTTCAAGAGAGAGCGGNGTCTGATCT
  CGTGTAGCCATATAGAGTTTGCTTCTGAGTGTCTGCTNTGTTTAGTAGAGGTGGGCAGGA
  GGAGCTGANGGGCTGGGGCTGGGGTGTTGAAGTTGGCTTTGCATGCCCAGCGATGCGCCT
  NCCTGTGGGATGTCATCACCCTGGGAACCCCGGAGTGGCCCTTGGCTCACTGTGTTCTGC
  ATGGNTGGNATCTGAATTAATTGCCTTTCTTCTAATCCCAACCGACTTCTTNCACCTCCA
  ACTGGCTGTACCCCAATCCAGCCATTACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005368 unedited
  TATGAACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTGATGTTGCAGG
  TTGTTTTATTAAAACCAGGTGAGTCACTGCCATACCTGAGGAGGCACACACAAGTTCCAG
  ATAGTCAAATGCTTCCCCAGCTGGCCCGCCCAGCCCAGATCTCCTCCTCATTGCAAAGGG
  ACATTCTGCTGTCTTCAAGGGGCCAGTGATTAATCAGACAATTGCTACTGTCAGGTGTAG
  TTAATGGCTTGGATTTGGGGTTACAGCCAGTTTGGAGGTTGGAAGAAGTTCGGTTGGGAT
  TTAGAAGAAAGGACAATTAATTCAGATCCAAACCATGCAGAACACAGTGAGCCAAGGGCC
  ACTCCCGGTTCCCAGGGTGATGACATCCCACAGGGAGGCGCATCGCTGGGCATGCAAAGC
  CAACTTCAACACCCCAGCCCCAGCCCCTCAGCTCCTCCTGCCCACCTCTACTAAACAAAG
  CAGACACTCAGAAGCAAACTCTATATGGCTACACGAGATCAGACCCCGCTCTCTCTTGAA
  CCCGGGGCCCANATGGGTGGGGGTGGGAGCGGCAGGGGCCTANCCCTGGAAGCCCAGCTC
  CTTGTAGTTGGAGGCCATGTCCTTCCGGAACAGCTCCAGGGCCTTGTTCATGGCCCCCTG
  NGCATCAGCACCAAAGTCCCCGGGATGCTTGCTCTGCAGAACCTGGATGATGCATTCCGA
  GATGAACTCCAGGTACTTCACGGGNGATCTTGTGCTNTGGTGGCATGCGACTGTGCCCAG
  GGGCTAATCTCTGCCTCATGATGCCCCTTCTTTCTAANGATGCCACCCAGGGCGGTGAGC
  ACAGTGGCACCATGCTTNCTTAAGNCCTCAGATGCCTTCATCTCGTCCTCTGACTTCAGG
  TGCCTNGAAACTGTCAAACTCTNCCGAGTCTCTGGGTGACCCCTTAAGACCTGATGAAGA
  CTTTCTGCCATGGCCTGTGGATGTCAGNCTCACCTTCCCCAGACGTTAGCACAACTGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005368
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005368.2, NP_005359.1
• RefSeq Size: 1078 bp
• RefSeq ORF: 465 bp
• Locus ID: 4151
• Cytogenetics: 22q12.3
• Domains: globin
• Gene Summary: 'This gene encodes a member of the globin superfamily and is predominantly expressed in skeletal and cardiac muscles. The encoded protein forms a monomeric globular haemoprotein that is primarily responsible for the storage and facilitated transfer of oxygen from the cell membrane to the mitochondria. This protein also plays a role in regulating physiological levels of nitric oxide. Multiple transcript variants encoding distinct isoforms exist for this gene. [provided by RefSeq, May 2020]'
  Transcript Variant: This variant (1) represents the shortest variant. Variants 1-4 encode the same protein.