n-Myc (MYCN) (NM_005378) Human Untagged Clone

CAT#: SC116780

MYCN (untagged)-Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYCN
• Synonyms: bHLHe37; MODED; N-myc; NMYC; ODED
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116780 sequence for NM_005378 edited (data generated by NextGen Sequencing)
  ATGCCGAGCTGCTCCACGTCCACCATGCCGGGCATGATCTGCAAGAACCCAGACCTCGAG
  TTTGACTCGCTACAGCCCTGCTTCTACCCGGACGAAGATGACTTCTACTTCGGCGGCCCC
  GACTCGACCCCCCCGGGGGAGGACATCTGGAAGAAGTTTGAGCTGCTGCCCACGCCCCCG
  CTGTCGCCCAGCCGTGGCTTCGCGGAGCACAGCTCCGAGCCCCCGAGCTGGGTCACGGAG
  ATGCTGCTTGAGAACGAGCTGTGGGGCAGCCCGGCCGAGGAGGACGCGTTCGGCCTGGGG
  GGACTGGGTGGCCTCACCCCCAACCCGGTCATCCTCCAGGACTGCATGTGGAGCGGCTTC
  TCCGCCCGCGAGAAGCTGGAGCGCGCCGTGAGCGAGAAGCTGCAGCACGGCCGCGGGCCG
  CCAACCGCCGGTTCCACCGCCCAGTCCCCGGGAGCCGGCGCCGCCAGCCCTGCGGGTCGC
  GGGCACGGCGGGGCTGCGGGAGCNGGCCGCGCCNNNNNNNNNCTGCCCGCCGAGCTCGCC
  CACCCGGCCGCCGAGTGCGTGGATCCCGCCGTGGTCTTCCCNNNNNNNGTGAACAAGCGC
  GAGCCAGCGCCCGTGCCCGCAGCCCCGGCCAGTGCCCCGGCGGCGGGCCCTGCGGTCGCC
  TCGGGGGCGGGTATTGCCGCCCCAGCCGGGGCCCCGGGGGTCGCCCCTCCGCGCCCAGGC
  GGCCGCCAGACCAGCGGCGGCGACCACAAGGCCCTCAGTACCTCCGGAGAGGACACCCTG
  AGCGATTCAGATGATGAAGATGATGAAGAGGAAGATGAAGAGGAAGAAATCGACGTGGTC
  ACTGTGGAGAAGCGGCGTTCCTCCTCCAACACCAAGGCTGTCACCACATTCACCATCACT
  GTGCGTCCCAAGAACGCAGCCCTGGGTCCCGGGAGGGCTCAGTCCAGCGAGCTGATCCTC
  AAACGATGCCTTCCCATCCACCAGCAGCACAACTATGCCGCCCCCTCTCCCTACGTGGAG
  AGTGAGGATGCACCCCCACAGAAGAAGATAAAGAGCGAGGCGTCCCCACGTCCGCTCAAG
  AGTGTCATCCCCCCAAAGGCTAAGAGCTTGAGCCCCCGAAACTCTGACTCGGAGGACAGT
  GAGCGTCGCAGAAACCACAACATCCTGGAGCGCCAGCGCCGCAACGACCTTCGGTCCAGC
  TTTCTCACGCTCAGGGACCACGTGCCGGAGTTGGTAAAGAATGAGAAGGCCGCCAAGGTG
  GTCATTTTGAAAAAGGCCACTGAGTATGTCCACTCCCTCCAGGCCGAGGAGCACCAGCTT
  TTGCTGGAAAAGGAAAAATTGCAGGCAAGACAGCAGCAGTTGCTAAAGAAAATTGAACAC
  GCTCGGACTTGCTAG
  
  Clone variation with respect to NM_005378.4
  504 c=>n;514 g=>n;515 g=>n;516 g=>n;517 g=>n;518 c=>n;519 c=>n;520 g=>n;521 c=>n;522 c=>n;582 c=>n;583 t=>n;584 t=>n;585 t=>n;586 c=>n;587 c=>n;588 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005378 unedited
  ATTAGGTCTACGACTCACTATAGGCGGCNCGCGAATCGGCACGAGGGTCGGTTGCAGTGT
  TGGAGGTCGGCGCCGGCCCCCGCCTTCCGCGCCCCCCACGGGAAGGAAGCACCCCCGGTA
  TTAAAACGAACGGGGCGGAAAGAAGCCCTCAGTCGCCGGCCGGGAGGCGAGCCGATGCCG
  AGCTGCTCCACGTCCACCATGCCGGGCATGATCTGCAAGAACCCAGACCTCGAGTTTGAC
  TCGCTACAGCCCTGCTTCTACCCGGACGAAGATGACTTCTACTTCGGCGGCCCCGACTCG
  ACCCCCCCGGGGGAGGACATCTGGAAGAAGTTTGAGCTGCTGCCCACGCCCCCGCTGTCG
  CCCAGCCGTGGCTTCGCGGAGCACAGCTCCGAGCCCCCGAGCTGGGTCACGGAGATGCTG
  CTTGAGAACGAGCTGTGGGGCAGCCCGGCCGAGGAGGACGCGTTCGGCCTGGGGGGACTG
  GGTGGCCTCACCCCCAACCCGGTCATCCTCCAGGACTGCATGTGGAGCGGCTTCTCCGCC
  CGCGAGAAGCTGGAGCGCGCCGTGAGCGAGAAGCTGCAGCACGGCCGCGGGCCGCCAACC
  GCCGGTTTCACCGCCCAGTCCCCCGGGAGCCGGCGCCGCCAGCCCTGCGGGTCGCGGGCA
  CGGTGGGGCTGCGGGAGCCGGCCGCGCCGGGGCCGACCTGCCCGCCGAGCTCGCCCACCC
  GGCCCGCGAGTGCGCGGATCCCGCCGAGGTCTTCCCCTTTCCTGTGAACAAGCGCGAGCC
  CGCGCCCGTGCCCGCATCCCCGCCCATGCCCCGGCGGCGGGCCCCTGCGGCGCCTTGGGG
  GCGGGTTATGACGCCCCAGCCGGGCCCCGAGGGTTGCCCCTCCCTGCCAGCGGCCGCCAA
  AAATCGTCGGCGACCCCAGGCCCTTGATCACTCGGCGAAGAACACTTGAGCGCATCTTAG
  CTCATATATATCCACGCAACCCGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005378 unedited
  NTTACCTTGGACGCGGCCGCATTCTANGATCGAGGTTTTTTTTTTTTTTTTTTTTTTTGC
  ATCATTTAATTTTAAGCTATTTATTTTCATAAACATGAGGTATTTCAAAGTGCTATAAGA
  TGCAGCACTAAATATATACATTTTGAAGAAATTAAACACAGAACTTTGCATTTACCCAGT
  TCTATGCACCAAACATGAACAAATACATTAACAGGAAGAAACAGGCTAGGAAAAAGGCAT
  ATATATATAGTAAATTTCTTTACAAAAGTTTCTTAGTTCAAAAAGTGATAAAGTAATATC
  TACTCAAAACTTTCACAACTCATTTTCATACGAAAATATAAGTATCAAATTTAGTTATGT
  ATCAGCGTCATACTAAAGTATACAGGCAGTGTAAGAATTAGTACAGTACATAACAGAGAT
  TAACAATATATTTGTATACAAAACATGCTCCTCAAACATTGAGGTATTATTACAGTACTT
  AGGTATGAACTTCCAGTCTAATACTGGCCGCAAAAGCCACCTCTCATTACCCAGGATGTA
  TACAAAAGGCGGATGTGTCAATGGTATTTACAGAAATGTTCCCCAGGGGTATCAAATGGC
  AAACCCCTTATGTGGCATCTGCTGGAACTTAAGCACCATTTTAAAAAGAAGGAATGACTT
  TCTGTTGTTTGGAAACTTGGAACACACAAGGTGACTTCAACAGCCCCAGAGGCTCCCAAC
  CATCACCAACGTTTAGCGCTGTCATGGAGGTGAGGTGGAGGAGGCTGCAAGGCCACCCAG
  CATCCTGGGATGCAGGCTCTCCAAGGGTCCAGCAGAACCCACACTGGTGGTCCTACTGGC
  CACCCAGAGCCGGACTCGACAGGGGAACGATTTGAAAGAAACCAACATTCTAAAGGCACA
  CCATGTTGGTGTAAAAAAATACAAATGTGCAAACGGCAGTGACTGCCACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005378
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005378.4, NP_005369.2
• RefSeq Size: 2613 bp
• RefSeq ORF: 1395 bp
• Locus ID: 4613
• Cytogenetics: 2p24.3
• Domains: HLH, Myc_N_term
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]'
  Transcript Variant: This variant (2) lacks segment 1b in the 5' region, compared to variant 1. This variant includes two open reading frames; the isoform 1 represented by this RefSeq is translated from the downstream open reading frame. This transcript and variant 1 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.