NFIL3 (NM_005384) Human Untagged Clone

CAT#: SC116782

NFIL3 (untagged)-Human nuclear factor, interleukin 3 regulated (NFIL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFIL3
• Synonyms: E4BP4; IL3BP1; NF-IL3A; NFIL3A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005384 edited
  GAATTCGGCACGAGGAAAGGAAGAATATTGATGGATTTTAAACCAGAGTTTTTAAAGAGC
  TTGAGAATACGGGGAAATTAATTTGTTCTCCTACACACATAGATAGGGTAAGGTTGTTTC
  TGATGCAGCTGAGAAAAATGCAGACCGTCAAAAAGGAGCAGGCGTCTCTTGATGCCAGTA
  GCAATGTGGACAAGATGATGGTCCTTAATTCTGCTTTAACGGAAGTGTCAGAAGACTCCA
  CAACAGGTGAGGAGCTGCTTCTCAGTGAAGGAAGTGTGGGGAAGAACAAATCTTCTGCAT
  GTCGGAGGAAACGGGAATTCATTCCTGATGAAAAGAAAGATGCTATGTATTGGGAAAAAA
  GGCGGAAAAATAATGAAGCTGCCAAAAGATCTCGTGAGAAGCGTCGACTGAATGACCTGG
  TTTTAGAGAACAAACTAATTGCACTGGGAGAAGAAAACGCCACTTTAAAAGCTGAGCTGC
  TTTCACTAAAATTAAAGTTTGGTTTAATTAGCTCCACAGCATATGCTCAAGAGATTCAGA
  AACTCAGTAATTCTACAGCTGTGTACTTTCAAGATTACCAGACTTCCAAATCCAATGTGA
  GTTCATTTGTGGACGAGCACGAACCCTCGATGGTGTCAAGTAGTTGTATTTCTGTCATTA
  AACACTCTCCACAAAGCTCGCTGTCCGATGTTTCAGAAGTGTCCTCAGTAGAACACACGC
  AGGAGAGCTCTGTGCAGGGAAGCTGCAGAAGTCCTGAAAACAAGTTCCAGATTATCAAGC
  AAGAGCCGATGGAATTAGAGAGCTACACAAGGGAGCCAAGAGATGACCGAGGCTCTTACA
  CAGCGTCCATCTATCAAAACTATATGGGGAATTCTTTCTCTGGGTACTCACACTCTCCCC
  CACTACTGCAAGTCAACCGATCCTCCAGCAACTCCCCGAGAACGTCGGAAACTGATGATG
  GTGTGGTAGGAAAGTCATCTGATGGAGAAGACGAGCAACAGGTCCCCAAGGGCCCCATCC
  ATTCTCCAGTTGAACTCAAGCATGTGCATGCAACTGTGGTTAAAGTTCCAGAAGTGAATT
  CCTCTGCCTTGCCACACAAGCTCCGGATCAAAGCCAAAGCCATGCAGATCAAAGTAGAAG
  CCTTTGATAATGAATTTGAGGCCACGCAAAAACTTTCCTCACCTATTGACATGACATCTA
  AAAGACATTTCGAACTCGAAAAGCATAGTGCCCCAAGTATGGTACATTCTTCTCTTACTC
  CTTTCTCAGTGCAAGTGACTAACATTCAAGATTGGTCTCTCAAATCGGAGCACTGGCATC
  AAAAAGAACTGAGTGGCAAAACTCAGAATAGTTTCAAAACTGGAGTTGTTGAAATGAAAG
  ACAGTGGCTACAAAGTTTCTGACCCAGAGAACTTGTATTTGAAGCAGGGGATAGCAAACT
  TATCTGCAGAGGTTGTCTCACTCAAGAGACTTATAGCCACACAACCAATCTCTGCTTCAG
  ACTCTGGGTAAATTACTACTGAGTAAGAGCTGGGCATTTAGAAAGATGTCATTTGCAATA
  GAGCAGTCCATTTTGTATTATGCTGAATTTTCACTGGACCTGTGATGTCATTTCACTGTG
  ATGTGCACATGTTGTCTGTTTGGTGTCTTTTTGTGCACAGATTATGATGAAGATTAGATT
  GTGTTATCACTCTGCCTGTGTATAGTCAGATAGTCCATGCGAAGGCTGTATATATTGAAC
  ATTATTTTTGTTGTTCTATTATAAAGTGTGTAAGTTACCAGTTTCAATAAAGGATTGGTG
  ACAAACACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACT
  CGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005384 unedited
  TGCAGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGAAGGAAG
  ATATTGATGGATTTTAAACCAGAGTTTTTAAAGAGCTTGAGATACGGGGAAATTAATTTG
  TTCTCCTACACACATAGATAGGGTAAGGTTGTTTCTGATGCAGCTGAGAAAAATGCAGAC
  CGTCAAAAAGGAGCAGGCGTCTCTTGATGCCAGTAGCAATGTGGACAAGATGATGGTCCT
  TAATTCTGCTTTAACGGAAGTGTCAGAAGACTCCACAACAGGTGAGGAGCTGCTTCTCAG
  TGAAGGAAGTGTGGGGAAGAACAAATCTTCTGCATGTCGGAGGAAACGGGAATTCATTCC
  TGATGAAAAGAAAGATGCTATGTATTGGGAAAAAAGGCGGAAAAATAATGAAGCTGCCAA
  AAGATCTCGTGAGAAGCGTCGACTGAATGACCTGGTTTTAGAGAACAAACTAATTGCACT
  GGGAGAAGAAAACGCCACTTTAAAAGCTGAGCTGCTTTCACTAAAATTAAAGTTTGGTTT
  AATTAGCTCCACAGCATATGCTCAAGAGATTCAGAAACTCAGTAATTCTACAGCTGTGTA
  CTTTCAAGATTACCAGACTTCCAAATCCAATGTGAGTTCATTTGTGGACGAGCACGAACC
  CTCGATGGTGTCAAGTAGTTGTATTTCTGTCATTAAACACTCTCCACANAGCTCGCTGTC
  CGATGTTTCAGAAGTGTCCTCAGTAGTACACACGCACGAGAGCTCTGTGCAGGGAAGCTG
  CANAAAGTCCTGAAACAAGTTCCAGATTTATCAAGCACGAGCCGATGGNATTAGAGAGCT
  ACACAAGGGACCCAGAGATGACCGAGCCTCTACACAGCGTTCATCTATCAAACTATATGG
  GGAATNCTTCTCTGGGTACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005384 unedited
  TTGTCCCAATCCTTTTGAACTGGTAACTTACACACTTTTAATAGAACAACAAAATAATGT
  TCAATATATACAGCCTTCGCATGGACTATCTGACTATACACAGGCAGAGTGATAACACAA
  TCTAATCTTCATCATAATCTGTGCACAAAAAGACACCAAACAGACAACATGTGCACATCA
  CAGTGAAATGACATCACAGGTCCAGTGAAAATTCAGCATAATACAAAATGGACTGCTCTA
  TTGCAAATGACATCTTTCTAAATGCCCAGCTCTTACTCAGTAGTAATTTACCCAGAGTCT
  GAAGCAGAGATTGGTTGTGTGGCTATAAGTCTCTTGAGTGAGACAACCTCTGCAGATAAG
  TTTGCTATCCCCTGCTTCAAATACAAGTTCTCTGGGTCAGAAACTTTGTAGCCACTGTCT
  TTCATTTCAACAACTCCAGTTTTGAAACTATTCTGAGTTTTGCCACTCAGTTCTTTTTGA
  TGCCAGTGCTCCGATTTGAGAGACCAATCTTGAATGTTAGTCACTTGCACTGAGAAAGGA
  GTAAGAGAAGAATGTACCATACTTGGGGCACTATGCTTTTCGAGTTCGAAATGTCTTTTA
  GATGTCATGTCAATAGGTGAGGAAAGTTTTTGCGTGGCCTCAAATTCATTATCAAAGGCT
  TCTACTTTGATCTGCATGGCTNTGGCTNTGATCCGGAGCTTGTGTGGCAGGCAGNAGAAT
  TCACTTCTGGAACTNTAACCACAGTTGCATGCACATGCTTGAGTTCAACTGGAGAATGGA
  TGGGNGCCCTTGNGGACCTGTTGCTCGTCTTCTCCATCAGATGACTTTCCTACCACACCA
  TCATCAGTTTNCCGACGTTCTCGGGGGAGTTGCTGGNAGGATCGGNTTGACTTNGCANTA
  NTGGGGGGAGAGTGTGAGTACCCAGAGAAAGAATTCCCCATATATTTTTGATAGATGGAC
  GCTGNGTTAGAAGCCCTCGTCATCTCTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005384
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005384.2, NP_005375.2
• RefSeq Size: 2104 bp
• RefSeq ORF: 1389 bp
• Locus ID: 4783
• Cytogenetics: 9q22.31
• Domains: BRLZ
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.