PODXL (NM_005397) Human Untagged Clone

CAT#: SC116789

PODXL (untagged)-Human podocalyxin-like (PODXL), transcript variant 2


  "NM_005397" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PODXL
Synonyms Gp200; PC; PCLP; PCLP-1
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_005397, the custom clone sequence may differ by one or more nucleotides


ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGC
CGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACC
GACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAG
GCCAACGAAATCTTGGCCTCGGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAA
CCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTAC
TACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA
GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCC
ACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACT
TAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATG
AAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCA
CCCTACCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCCAGTCAGATGCCAGCCAGCTCTACGGC
CCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGGCATTGAGAACACCTACCCTGCCAGAG
ACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGG
CTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCT
GAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATATGCCGA
GCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCATCTGTTCCAGGAAGTC
AGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCTGCCAAGGATGTGTACGAGCGGCTGAA
GGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAG
GAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCG
TGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGA
GCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCTGAGATG
CAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTCCCTCTGGACAACCTGA
CCAAGGACGACCTGGATGAGGAGGAAGACACACACCTCTAG


>OriGene 5' read for NM_005397 unedited
NTTTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA
TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG
CCGCGAATTCGCACGAGGCGCCGCAGCGCGACTCGGGAGCCCCGGGCCACAGCCTGGCCT
CCGGAGCCACCCACAGGCCTCCCCGGGCGGCGCCCACGCTCCTACCGCCCGGACGCGCGG
ATCCTCCGCCGGCACCGCAGCCACCTGCTCCCGGCCCAGAGGCGACGACACGATGCGCTG
CGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTC
GCCGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAA
CAAAACAGCACCGACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCA
GAGCACAGTCCCCACTTCCAAGGCCAACGAAATCTTGGCCTCAGTCAAGGCGACCACCCT
TGGTGTATCCAGTGACTCACCGGGGACTACAACCCTGGCTCAGCAAGTCTCAGGCCCAGT
CAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTACTACCACCATCGAGAGCCC
CAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACAGCTAAACC
TAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAG
CCACAGTGTGACCACAGACCTCACATCCACTAAGGGCAGACATCTGACGACCCCTCACCC
CTACAGTCCACTTAGCCCCCGACACCCCACTTGACGCATNCTGTGGCCACCCCCACAGCT
CGGGACATGACCATCTTATGAAANTTNCAAGCAGTTCAGCACTGG
>OriGene 3' read for NM_005397 unedited
NCGGTTTCGCTATGGNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTA
CACAAAAACACTTTAATTGACAGTATACAATTTTCCAAAATATATTTTTGTAAGAAAATG
CAATAATTATTAACTATAGTTTTTACAAACAAGTTTCTCAGTAAATTCCAGTGTACTTCA
GACCCCTGTCCACTAAGACATATATGATCCCCCAGTTCCTGGGCAAACTGTTGAACATTC
ACTGCAGACAAAAAGACCAACACCAAAGAGTCATCTGTGTCCTCCATGCTGTGTTTGCAG
CAGACCTGAGGGATCAGCTAGTGACCGTGACAAAAGCTATGCTACAGTTTTACTCTTGCC
CTCTCTGCCTCCCCCATCATGTTTCCTTGGTCCCTCAGTCCTGTTGGAGTAAGTTCCATA
TATAAAAGCTCATCCAAAACTTTGTTTCTTGGCAGAAAGAAAGAATTGTTCACTTACATC
TTAAACCTAAATGATTCAGAGAAAGTGTAGAAGACCAGTTCTTATGTTTGTGTTCATGCC
CTCCCTAGCCTTGCTAGGGTAACAACTGGGAAAGGAATTCTGTCCTTTGTGTCATTCTGG
CACCTTCGAGTAGCAATTCTTAATTTACCGATTATTCCTTCTGAGGCGCACAGTCTGTGG
ATCCAGGGTTTAAACTTGGTTCTGTCCTGAACCATGTGATTTTGAGGAAGTCACTTCATC
TTTGTGAGCTTCAAATTATCTTTTTCTCTTCAAAAAGAATGGACTAGATCTTGGAAAACC
TTTCTCCATGACTACAGGGGGTGGCTTGCCACCCAAGCATATTGGCTCCAACCTTTACTG
GAAATGGAGCTGGGACCTTTCTGGGGAGGGGGGCAGGGGTTGGCTTTTAAAATTAAN
Restriction Sites NotI-NotI     
ACCN NM_005397
Insert Size 6000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005397.2, NP_005388.2
RefSeq Size 5903 bp
RefSeq ORF 1581 bp
Locus ID 5420
Cytogenetics 7q32.3
Protein Families Transmembrane
Gene Summary 'This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) lacks an in-frame alternate exon compared to variant 1, resulting in a shorter protein (isoform 2).

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