PODXL (NM_005397) Human Untagged Clone

CAT#: SC116789

PODXL (untagged)-Human podocalyxin-like (PODXL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PODXL
• Synonyms: Gp200; PC; PCLP; PCLP-1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005397, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGC
  CGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACC
  GACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAG
  GCCAACGAAATCTTGGCCTCGGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAA
  CCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTAC
  TACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA
  GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCC
  ACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACT
  TAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATG
  AAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCA
  CCCTACCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCCAGTCAGATGCCAGCCAGCTCTACGGC
  CCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGGCATTGAGAACACCTACCCTGCCAGAG
  ACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGG
  CTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCT
  GAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATATGCCGA
  GCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCATCTGTTCCAGGAAGTC
  AGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCTGCCAAGGATGTGTACGAGCGGCTGAA
  GGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAG
  GAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCG
  TGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGA
  GCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCTGAGATG
  CAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTCCCTCTGGACAACCTGA
  CCAAGGACGACCTGGATGAGGAGGAAGACACACACCTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005397 unedited
  NTTTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA
  TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG
  CCGCGAATTCGCACGAGGCGCCGCAGCGCGACTCGGGAGCCCCGGGCCACAGCCTGGCCT
  CCGGAGCCACCCACAGGCCTCCCCGGGCGGCGCCCACGCTCCTACCGCCCGGACGCGCGG
  ATCCTCCGCCGGCACCGCAGCCACCTGCTCCCGGCCCAGAGGCGACGACACGATGCGCTG
  CGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTC
  GCCGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAA
  CAAAACAGCACCGACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCA
  GAGCACAGTCCCCACTTCCAAGGCCAACGAAATCTTGGCCTCAGTCAAGGCGACCACCCT
  TGGTGTATCCAGTGACTCACCGGGGACTACAACCCTGGCTCAGCAAGTCTCAGGCCCAGT
  CAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTACTACCACCATCGAGAGCCC
  CAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACAGCTAAACC
  TAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAG
  CCACAGTGTGACCACAGACCTCACATCCACTAAGGGCAGACATCTGACGACCCCTCACCC
  CTACAGTCCACTTAGCCCCCGACACCCCACTTGACGCATNCTGTGGCCACCCCCACAGCT
  CGGGACATGACCATCTTATGAAANTTNCAAGCAGTTCAGCACTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005397 unedited
  NCGGTTTCGCTATGGNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTA
  CACAAAAACACTTTAATTGACAGTATACAATTTTCCAAAATATATTTTTGTAAGAAAATG
  CAATAATTATTAACTATAGTTTTTACAAACAAGTTTCTCAGTAAATTCCAGTGTACTTCA
  GACCCCTGTCCACTAAGACATATATGATCCCCCAGTTCCTGGGCAAACTGTTGAACATTC
  ACTGCAGACAAAAAGACCAACACCAAAGAGTCATCTGTGTCCTCCATGCTGTGTTTGCAG
  CAGACCTGAGGGATCAGCTAGTGACCGTGACAAAAGCTATGCTACAGTTTTACTCTTGCC
  CTCTCTGCCTCCCCCATCATGTTTCCTTGGTCCCTCAGTCCTGTTGGAGTAAGTTCCATA
  TATAAAAGCTCATCCAAAACTTTGTTTCTTGGCAGAAAGAAAGAATTGTTCACTTACATC
  TTAAACCTAAATGATTCAGAGAAAGTGTAGAAGACCAGTTCTTATGTTTGTGTTCATGCC
  CTCCCTAGCCTTGCTAGGGTAACAACTGGGAAAGGAATTCTGTCCTTTGTGTCATTCTGG
  CACCTTCGAGTAGCAATTCTTAATTTACCGATTATTCCTTCTGAGGCGCACAGTCTGTGG
  ATCCAGGGTTTAAACTTGGTTCTGTCCTGAACCATGTGATTTTGAGGAAGTCACTTCATC
  TTTGTGAGCTTCAAATTATCTTTTTCTCTTCAAAAAGAATGGACTAGATCTTGGAAAACC
  TTTCTCCATGACTACAGGGGGTGGCTTGCCACCCAAGCATATTGGCTCCAACCTTTACTG
  GAAATGGAGCTGGGACCTTTCTGGGGAGGGGGGCAGGGGTTGGCTTTTAAAATTAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005397
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005397.2, NP_005388.2
• RefSeq Size: 5903 bp
• RefSeq ORF: 1581 bp
• Locus ID: 5420
• Cytogenetics: 7q32.3
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame alternate exon compared to variant 1, resulting in a shorter protein (isoform 2).