GRB7 (NM_005310) Human Untagged Clone

CAT#: SC116816

GRB7 (untagged)-Human growth factor receptor-bound protein 7 (GRB7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRB7
• Synonyms: growth factor receptor-bound protein 7; OTTHUMP00000164352
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116816 sequence for NM_005310 edited (data generated by NextGen Sequencing)
  ATGGAGCTGGATCTGTCTCCACCTCATCTTAGCAGCTCTCCGGAAGACCTTTGCCCAGCC
  CCTGGGACCCCTCCTGGGACTCCCCGGCCCCCTGATACCCCTCTGCCTGAGGAGGTAAAG
  AGGTCCCAGCCTCTCCTCATCCCAACCACCGGCAGGAAACTTCGAGAGGAGGAGAGGCGT
  GCCACCTCCCTCCCCTCTATCCCCAACCCCTTCCCTGAGCTCTGCAGTCCTCCCTCACAG
  AGCCCAATTCTCGGGGGCCCCTCCAGTGCAAGGGGGCTGCTCCCCCGCGATGCCAGCCGC
  CCCCATGTAGTAAAGGTGTACAGTGAGGATGGGGCCTGCAGGTCTGTGGAGGTGGCAGCA
  GGTGCCACAGCTCGCCACGTGTGTGAAATGCTGGTGCAGCGAGCTCACGCCTTGAGCGAC
  GAGACCTGGGGGCTGGTGGAGTGCCACCCCCACCTAGCACTGGAGCGGGGTTTGGAGGAC
  CACGAGTCCGTGGTGGAAGTGCAGGCTGCCTGGCCCGTGGGCGGAGATAGCCGCTTCGTC
  TTCCGGAAAAACTTCGCCAAGTACGAACTGTTCAAGAGCTCCCCACACTCCCTGTTCCCA
  GAAAAAATGGTCTCCAGCTGTCTCGATGCACACACTGGTATATCCCATGAAGACCTCATC
  CAGAACTTCCTGAATGCTGGCAGCTTTCCTGAGATCCAGGGCTTTCTGCAGCTGCGGGGT
  TCAGGACGGAAGCTTTGGAAACGCTTTTTCTGCTTCTTGCGCCGATCTGGCCTCTATTAC
  TCCACCAAGGGCACCTCTAAGGATCCGAGGCACCTGCAGTACGTGGCAGATGTGAACGAG
  TCCAACGTGTACGTGGTGACGCAGGGCCGCAAGCTCTACGGGATGCCCACTGACTTCGGT
  TTCTGTGTCAAGCCCAACAAGCTTCGAAATGGCCACAAGGGGCTTCGGATCTTCTGCAGT
  GAAGATGAGCAGAGCCGCACCTGCTGGCTGGCTGCCTTCCGCCTCTTCAAGTACGGGGTG
  CAGCTGTACAAGAATTACCAGCAGGCACAGTCTCGCCATCTGCATCCATCTTGTTTGGGC
  TCCCCACCCTTGAGAAGTGCCTCAGATAATACCCTGGTGGCCATGGACTTCTCTGGCCAT
  GCTGGGCGTGTCATTGAGAACCCCCGGGAGGCTCTGAGTGTGGCCCTGGAGGAGGCCCAG
  GCCTGGAGGAAGAAGACAAACCACCGCCTCAGCCTGCCCATGCCAGCCTCCGGCACGAGC
  CTCAGTGCAGCCATCCACCGCACCCAACTCTGGTTCCACGGGCGCATTTCCCGTGAGGAG
  AGCCAGCGGCTTATTGGACAGCAGGGCTTGGTAGACGGCCTGTTCCTGGTCCGGGAGAGT
  CAGCGGAACCCCCAGGGCTTTGTCCTCTCTTTGTGCCACCTGCAGAAAGTGAAGCATTAT
  CTCATCCTGCCGAGCGAGGAGGAGGGCCGCCTGTACTTCAGCATGGATGATGGCCAGACC
  CGCTTCACTGACCTGCTGCAGCTCGTGGAGTTCCACCAGCTGAACCGCGGCATCCTGCCG
  TGCTTGCTGCGCCATTGCTGCACGCGGGTGGCCCTCTGA
  
  Clone variation with respect to NM_005310.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005310 unedited
  AGGTTGCGGATTAGTATACGATCCTATAGGGCGGCCGCGATTCGGCACGAGGGTCCCAGC
  CGGGTGTCCTGACGCTCGGGGTTCAGGACAAGGGCACACAACTGGTTCCGTTAAGCCCCT
  CTCTCGCTCAGACGCCATGGAGCTGGATCTGTCTCCACCTCATCTTAGCAGCTCTCCGGA
  AGACCTTTGCCCAGCCCCTGGGACCCCTCCTGGGACTCCCCGGCCCCCTGATACCCCTCT
  GCCTGAGGAGGTAAAGAGGTCCCAGCCTCTCCTCATCCCAACCACCGGCAGGAAACTTCG
  AGAGGAGGAGAGGCGTGCCACCTCCCTCCCCTCTATCCCCAACCCCTTCCCTGAGCTCTG
  CAGTCCTCCCTCACAGAGCCCAATTCTCGGGGGCCCCTCCAGTGCAAGGGGGCTGCTCCC
  CCGCGATGCCAGCCGCCCCCATGTAGTAAAGGTGTACAGTGAGGATGGGGCCTGCAGGTC
  TGTGGAGGTGGCAGCAGGTGCCACAGCTCGCCACGTGTGTGAAATGCTGGTGCAGCGAGC
  TCACGCCTTGAGCGACGAGACCTGGGGGCTGGTGGAGTGCCACCCCCACCTAGCACTGGA
  GCGGNGTTTGGAGGACCACGAGTCCGTGGTGGAAGTGCAGGCTGCCTGGCCCGTGGGCGG
  AGATAGCCGCTTCGTCTTCCGGAAAAACTTCGCCAAGTACGAACTGTTCAAGAGCTCCCC
  CACTCCCTGTTCCCAGAAAAAAGTTCTCCAGCTGTCTCGATGCACACACTGGTATATCCC
  ATGAAAACCTCATCCAGAACTTCCTGAATGCTGGCAGCTTTCCTGAGATCCAGGGCTTTT
  CTGCAGCTGCCGGGTTCAAGACGGGAGCCTCTGGAACCGCTNTTTTTGCTTCTTGCGCCG
  AACTGGCCCNTATGACTCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005310 unedited
  GGCACGCATTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTACAAAAAAAATCTTTA
  TTGTCACTAGTATAAAACAGAGCAGATCAACTGGCCTCTCGGTCTGTACAAAGTGTGGGG
  CGTGAAACCGCCTGGGCTGCCCCCACTTCTCCCATAATTCCCTGCCCTAGAGCAGCACCT
  CCAGAGCTAGGAGAAGGAGAGGGGGCCACCCAAGGCCTTCCCTTGAGGAGAGGGGTCAGG
  AGTGGACTGGAGTGGGGGCTGTTTTCTATCTGAGGGAGGCAAAGAAGCACAGGAGAAAAC
  TGGAGTGGCGGAACCCTACCGATCCTCATACCGTACCCTGTGGCCGCGCCCCAAAGTCCA
  CTGGATGGGTGGAGGGGCGGCGGCCACCCTGAAGGCGGACTGAGGCATGAGCCAATCCAC
  GGATCTGGTCAGAGGGCCACCCGCGTGCACCAATGGCGCACCAAGCACGGCCGGATGCCG
  CGGTTCACCTGGTGGAACTCCACCAGCTGCACCAGGCCATCGAAACGGCCCTGGCCATCA
  TACATGCTGAAGCACAGGCGAGCCCCCCTATCTCCTCGGCAGGATGAGATAATGCCCCAC
  TCCCTGCAGGCGGCCTACACAGAGGACAACACACTGAGGGACATCGCTGCCCCTTCCCGG
  TCCTAGAACAGTATGCCTCTCAACCCCCGATGCCACAACAACCCCTTGCCTCCCTCACAC
  GGTAAATGCCCACCTGACACCAAAAACTGCCGCCGCGGCAGGCCCGCCATGAGGCTACCG
  CCCGAAGCTGCCATGGACAGGTTGAGACGGGGACAACCAATTATCATCCAAACCGGCACT
  CCAACAGGCCACACTCCTACCACCCGGGGGTTCCAATGACCCCTCCCCTGGCTCCCATAT
  CCCTGCCCCCAGGACTCTTCCGGGGCACCCCTCAGGGGGGACGCCCACAAAAGGCTCCCC
  GGGAACCCGCCCCCCGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005310
• Insert Size: 2130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005310.2, NP_005301.2
• RefSeq Size: 2260 bp
• RefSeq ORF: 1599 bp
• Locus ID: 2886
• Cytogenetics: 17q12
• Domains: RA, SH2, PH
• Protein Families: Druggable Genome, Embryonic stem cells, Stem cell - Pluripotency
• Gene Summary: 'The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (a) is shorter at the N-terminus compared to isoform b. Variants 1, 2, and 3 all encode the same isoform (a).