ERp57 (PDIA3) (NM_005313) Human Untagged Clone

CAT#: SC116818

PDIA3 (untagged)-Human protein disulfide isomerase family A, member 3 (PDIA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDIA3
• Synonyms: ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HEL-S-93n; HEL-S-269; HsT17083; P58; PI-PLC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116818 sequence for NM_005313 edited (data generated by NextGen Sequencing)
  ATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCTTCTTGCCGCGGCCCGCCTCGCCGCTG
  CCTCCGACGTGCTAGAACTCACGGACGACAACTTCGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCT
  CATGCTCGTCGAGTTCTTCGCTCCCTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCA
  GCTACCAGATTAAAAGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATA
  AATATGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTATGATGG
  ACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGCTTCAGTGCCTCTCAGG
  ACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTCTATAGTAGGTTTTTTCGATGATTCAT
  TCAGTGAGGCTCACTCCGAGTTCCTAAAAGCAGCCAGCAACTTGAGGGATAACTACCGATTTGCACATAC
  GAATGTTGAGTCTCTGGTGAACGAGTATGATGATAATGGAGAGGGTATCATCTTATTTCGTCCTTCACAT
  CTCACTAACAAGTTTGAGGACAAGACTGTGGCATATACAGAGCAAAAAATGACCAGTGGCAAAATTAAAA
  AGTTTATCCAGGAAAACATTTTTGGTATCTGCCCTCACATGACAGAAGACAATAAAGATTTGATACAGGG
  CAAGGACTTACTTATTGCTTACTATGATGTGGACTATGAAAAGAACGCTAAAGGTTCCAACTACTGGAGA
  AACAGGGTAATGATGGTGGCAAAGAAATTCCTGGATGCTGGGCACAAACTCAACTTTGCTGTAGCTAGCC
  GCAAAACCTTTAGCCATGAACTTTCTGATTTTGGCTTGGAGAGCACTGCTGGAGAGATTCCTGTTGTTGC
  TATCAGAACTGCTAAAGGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGTGATGGGAAGGCTCTGGAG
  AGGTTCCTGCAGGATTACTTTGATGGCAATCTGAAGAGATACCTGAAGTCTGAACCTATCCCAGAGAGCA
  ATGATGGGCCTGTGAAGGTAGTGGTAGCAGAGAATTTTGATGAAATAGTGAATAATGAAAATAAAGATGT
  GCTGATTGAATTTTATGCCCCTTGGTGTGGTCATTGTAAGAACCTGGAGCCCAAGTATAAAGAACTTGGC
  GAGAAGCTCAGCAAAGACCCAAATATCGTCATAGCCAAGATGGATGCCACAGCCAATGATGTGCCTTCTC
  CATATGAAGTCAGAGGTTTTCCTACCATATACTTCTCTCCAGCCAACAAGAAGCTAAATCCAAAGAAATA
  TGAAGGTGGCCGTGAATTAAGTGATTTTATTAGCTATCTACAAAGAGAAGCTACAAACCCCCCTGTAATT
  CAAGAAGAAAAACCCAAGAAGAAGAAGAAGGCACAGGAGGATCTCTAA
  
  Clone variation with respect to NM_005313.4
  46 c=>n;47 t=>n;48 t=>n;49 g=>n;50 c=>n;51 c=>n;52 g=>n;53 c=>n;162 c=>t;1224 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005313 unedited
  CGGTGCAGATTTGTATACGACTCACTATAGGCGGCCGCGCAATCGGCACGAGGGCGGGTT
  AGTGGTCGCGCGCCCGACCTCCGCAGTCCCAGCCGAGCCGCGACCCTTCCGGCCGTCCCC
  ACCCCACCTCGCCGCCATGCGCCTCCGCCGCCTAGCGCTGTTCCCGGGTGTGGCGCTGCT
  TCTTGCCGCGGCCCGCCTCGCCGCTGCCTCCGACGTGCTAGAACTCACGGACGACAACTT
  CGAGAGTCGCATCTCCGACACGGGCTCTGCGGGCCTCATGCTCGTCGAGTTCTTCGCTCC
  CTGGTGTGGACACTGCAAGAGACTTGCACCTGAGTATGAAGCTGCAGCTACCAGATTAAA
  AGGAATAGTCCCATTAGCAAAGGTTGATTGCACTGCCAACACTAACACCTGTAATAAATA
  TGGAGTCAGTGGATATCCAACCCTGAAGATATTTAGAGATGGTGAAGAAGCAGGTGCTTA
  TGATGGACCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAGCAGGCAGGACCAGC
  TTCAGTGCCTCTCAGGACTGAGGAAGAATTTAAGAAATTCATTAGTGATAAAGATGCCTC
  TATAGTAGGTTTTTTCGATGATTCATTCAGTGAGGCTCACTCCGAGTTCCTAGAAGCAGC
  CAGCAACTTGAGGGATAACTACCGATTTGCACATACCAATGTTGAGTCTCTGGTGAACGA
  GTATGATGATCATGGAGCAGGGATCATCTTATTTCGTCCTTCACATCTCACTAACCAAGT
  TTGAAGACAAGACTGTGGCATATACCGAGCCACAAATGACCCGTGGCAACATTTAACAGG
  TTATCCCGGACAACATTTTTGGTATCTGCCCTCCCTTGACCGAAGACACTTCGATTTGTT
  CCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005313 unedited
  GAGCTTAAGGGGTCTCATTTTTGTTACTGTTCCAAAGTACAAAAAAAATAGAAAATACCC
  CCAACTCAACCCCACCCCCNACAACATTTCCCCCAACACAAATAATTTTTCCCAAAACCA
  CAAACATAAACTGGTCCTGGTATTTCCATAAACAGTGCAGCTAAGAAACAGTTTAGAGAA
  AATTTAACAGGATTCAGATTATATCCATTCTGTCCTCTCGGCCCTGAGAGGTAATAATTC
  CCATATGGGTCCTAGTCCTCCCCAATGGTTTTCCCATCTCTGATGGAAGAGTCCTTTTAC
  AAAGTGGTGTTTGGCTACTGCTTTAGAGATCCTCCTGTGCCTTCTTCTTCTTCTTGGGTT
  TTTCTTCTTGAATTACAGGGGGGTTTGTAGCTTCTCTTTGTAGATAGCTAATAAAATCAC
  TTAATTCACGGCCACCTTCATATTTCTTTGGATTTAGCTTCTTGTTGGCTGGAGAGAAGT
  ATATGGTAGGAAAACCTCTGACTTCATATGGAGAAGGCACATCATTGGCTGTGGCATCCA
  TCTTGGCTATGACGATATTTGGGTCTTTGCTGAGCTTCTCGCCAAGTTCTTTATACTTGG
  GCTCCAGGTTCTTACAATGACCACACCAAGGGGCATAAAATTCAATCAGCACATCTTTAT
  TTTCATTATTCACTATTTCATCAAAATTCTCTGCTACCACTACCTTCACAGGCCCATCAT
  TGCTCTCTGGGATAGGTTCAGACTTCAGGTATCTCTTCAGATTGCCATCAAAGTAATCCT
  GCAGGGACCTCTNCAGAGCCTTNCCATCACGCGAGAACTCCTCCTGCATGACAAACTTTT
  CTTCCTTAGCAGTTCTGATAAGCACAACAGGGAATCTCTTCAGCAGTGCTCTTCAAGCCA
  AAATCAGAAAGTTCATGGCTTAAGGGGTTTGCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005313
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005313.4, NP_005304.3
• RefSeq Size: 3060 bp
• RefSeq ORF: 1518 bp
• Locus ID: 2923
• Cytogenetics: 15q15.3
• Domains: thiored
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation
• Gene Summary: 'This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]'