Hyaluronan synthase 2 (HAS2) (NM_005328) Human Untagged Clone

CAT#: SC116826

HAS2 (untagged)-Human hyaluronan synthase 2 (HAS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HAS2
• Synonyms: MGC126241; MGC126242
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005328 edited
  ATGCATTGTGAGAGGTTTCTATGTATCCTGAGAATAATTGGAACCACACTCTTTGGAGTC
  TCTCTCCTCCTTGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACGGAT
  AATTACTATTTCTCTTTTGGACTGTATGGTGCCTTTTTGGCATCACACCTCATCATCCAA
  AGCCTGTTTGCCTTTTTGGAGCACCGAAAAATGAAAAAATCCCTAGAAACCCCCATAAAG
  TTGAACAAAACAGTTGCCCTTTGCATCGCTGCCTATCAAGAAGATCCAGACTACTTAAGG
  AAATGTTTGCAATCTGTGAAAAGGCTAACCTACCCTGGGATTAAAGTTGTCATGGTCATA
  GATGGGAACTCAGAAGATGACCTTTACATGATGGACATCTTCAGTGAAGTCATGGGCAGA
  GACAAATCAGCCACTTATATCTGGAAGAACAACTTCCACGAAAAGGGTCCCGGTGAGACA
  GATGAGTCACATAAAGAAAGCTCGCAACACGTAACGCAATTGGTCTTGTCCAACAAAAGT
  ATCTGCATCATGCAAAAATGGGGTGGAAAAAGAGAAGTCATGTACACAGCCTTCAGAGCA
  CTGGGACGAAGTGTGGATTATGTACAGGTTTGTGATTCAGACACTATGCTTGACCCAGCC
  TCATCTGTGGAGATGGTAAAAGTTTTAGAAGAAGATCCCATGGTTGGAGGTGTTGGGGGA
  GATGTCCAGATTTTAAACAAGTACGATTCCTGGATCTCATTCCTCAGCAGTGTAAGATAT
  TGGATGGCTTTTAATATAGAAAGGGCCTGTCAGTCTTATTTTGGGTGTGTTCAGTGCATT
  AGTGGACCTCTGGGAATGTACAGAAACTCCTTGTTGCATGAGTTTGTGGAAGATTGGTAC
  AATCAAGAATTTATGGGCAACCAATGTAGCTTTGGTGATGACAGGCATCTCACGAACCGG
  GTGCTGAGCCTGGGCTATGCAACAAAATACACAGCTCGATCTAAGTGCCTTACTGAAACA
  CCTATAGAATATCTCAGATGGCTAAACCAGCAGACCCGTTGGAGCAAGTCCTACTTCCGA
  GAATGGCTGTACAATGCAATGTGGTTTCACAAACATCACTTGTGGATGACCTACGAAGCG
  ATTATCACTGGATTCTTTCCTTTCTTTCTCATTGCCACAGTAATCCAGCTCTTCTACCGG
  GGTAAAATTTGGAACATTCTCCTCTTCTTGTTAACTGTCCAGCTAGTAGGTCTCATAAAA
  TCATCTTTTGCCAGCTGCCTTAGAGGAAATATCGTCATGGTCTTCATGTCTCTCTACTCA
  GTGTTATACATGTCGAGTTTACTTCCCGCCAAGATGTTTGCAATTGCAACAATAAACAAA
  GCTGGGTGGGGCACATCAGGAAGGAAAACCATTGTTGTTAATTTCATAGGACTCATTCCA
  GTATCAGTTTGGTTTACAATCCTCCTGGGTGGTGTGATTTTCACCATTTATAAGGAGTCT
  AAAAGGCCATTTTCAGAATCCAAACAGACAGTTCTAATTGTTGGAACGTTGCTCTATGCA
  TGCTATTGGGTCATGCTTTTGACGCTGTATGTAGTTCTCATCAATAAGTGTGGCAGGCGG
  AAGAAGGGACAACAATATGACATGGTGCTTGATGTATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005328 unedited
  CCATTCCCCCGCCCCGCTGCGCATTGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATAA
  GCAGTTTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGAAGAGTTGAACCACCTAAGGCGGAGTTCAAACAAAGGCTGAAGC
  AGGAAGGTTTGCTGGGGGAAATCAGTGGGGTCCCCTGAGCAGCGTGAAGTCATCAGGCCA
  AGGTCGTCTCAAATTCATCTGATCTCTTATTACCTCAATTTTGGAAACTGCCCGCCACCG
  ACCCTCCGGGACCACACAGACAGGCTGAGGACGACTTTATGACCAAGAGCTGAACAAGAT
  GCATTGTGAGAGGTTTCTATGTATCCTGAGAATAATTGGAACCACACTCTTTGGAGTCTC
  TCTCCTCCTTGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACGGATAA
  TTACTATTTCTCTTTTGGACTGTATGGTGCCTTTTTGGCATCACACCTCATCATCCAAAG
  CCTGTTTGCCTTTTTGGAGCACCGAAAAATGAAAAAATCCCTAGAAACCCCCATAAAGTT
  GAACAAAACAGTTGCCCTTTGCATCGCTGCCTATCAAGAAGAGCCAGACTACTTAAGGAA
  ATGTTTGCAATCTGTGAAAAGGCTAACCTACCCTGNGATTAAAGTTGTCATGGTCATAGA
  TGGGAACTCAGCAGATGACCTTTACATGATGGACATCTTCAGTGAAGTCATGGGCAGAGA
  CTAATCAGCCACTTATATCTGGAAGACAACTTCCCGAAAAGGGTCCCTGTGAGACAGATG
  AGTCACATACAGCAAGCTCGCTACACGTAACGCTAATTGGTCTTGTCCTACACCAGTATC
  TGCATCATGCTAAAATGTNNGTGGGACAAGAGCAGTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005328 unedited
  CCGGAAATACTATTACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCTTA
  TCAAAAATATTTTATTTACAAAAAATAATTATACTATACATCCTATACTGGAAATACATT
  GAATAATTGCTAAAATAAATACAGGCAATTAATTCAACTTTTTATAAGAATGAGAGAATT
  TGACATTTGAATGTTATCAAAGCTTAACTTAGAACATAATTAGTTAAAAAGGCAAACTCA
  AGTTTTCATCTCATTTATTGGCATGGTGTGTTTTGTTTTCCACAAGCTCTCCAATCAGTG
  AGAGTCTTGAGTCTCAGATGTACCTTTTTGTACTCTGAAGGTAGGATGTGCAGCTTTTTC
  TTAGGCAGGATAGATGTAACATAGATGACTGCCTAAAAAAGAGGCAGAAAGGCAGGAAGC
  CCATTTTTTTTTTAACACTTCTCCATGTGTCTCTGCCTCACCATAATGCTGTTTATTCAA
  TTATTCATATATTCCGATTGTGTTTTCAACACAGGTTTACACAGCACCAGCTACAAGGCA
  AGGTCAATCGTGTAAAAAATAAAACTGGATAATGGAGGCCTTGCAGTAACTAAATTAGCC
  AAGTTCAGAGTATGTGGTTCAACAATTTTTATATGTGCCCATCATTGATATACTTATTAA
  ACCTTGATGTCACTTCTACTGGGGAGACATTTTTATTCAGGTCCACAACAGGAAATAAGA
  AGTGATGATCTGTGTAATTCACAAAAACAAATTCCTCCTTTGTAAAGTAAAAGAGGAAAG
  AAACTTGGTGGAGTCTTCTTGCCCAATTTCAAATTATATGGTTTTAAAAATTATCCCACT
  TCACCCGGGTAGACCTTTTTACCTCCCTGGTAAAGGGAACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005328
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005328.1, NP_005319.1
• RefSeq Size: 3003 bp
• RefSeq ORF: 1659 bp
• Locus ID: 3037
• Cytogenetics: 8q24.13
• Domains: Glycos_transf_2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: ' Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]'