Hyaluronan synthase 2 (HAS2) (NM_005328) Human Untagged Clone

CAT#: SC116826

HAS2 (untagged)-Human hyaluronan synthase 2 (HAS2)


  "NM_005328" in other vectors (6)

Reconstitution Protocol

USD 540.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HAS2
Synonyms MGC126241; MGC126242
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_005328 edited
ATGCATTGTGAGAGGTTTCTATGTATCCTGAGAATAATTGGAACCACACTCTTTGGAGTC
TCTCTCCTCCTTGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACGGAT
AATTACTATTTCTCTTTTGGACTGTATGGTGCCTTTTTGGCATCACACCTCATCATCCAA
AGCCTGTTTGCCTTTTTGGAGCACCGAAAAATGAAAAAATCCCTAGAAACCCCCATAAAG
TTGAACAAAACAGTTGCCCTTTGCATCGCTGCCTATCAAGAAGATCCAGACTACTTAAGG
AAATGTTTGCAATCTGTGAAAAGGCTAACCTACCCTGGGATTAAAGTTGTCATGGTCATA
GATGGGAACTCAGAAGATGACCTTTACATGATGGACATCTTCAGTGAAGTCATGGGCAGA
GACAAATCAGCCACTTATATCTGGAAGAACAACTTCCACGAAAAGGGTCCCGGTGAGACA
GATGAGTCACATAAAGAAAGCTCGCAACACGTAACGCAATTGGTCTTGTCCAACAAAAGT
ATCTGCATCATGCAAAAATGGGGTGGAAAAAGAGAAGTCATGTACACAGCCTTCAGAGCA
CTGGGACGAAGTGTGGATTATGTACAGGTTTGTGATTCAGACACTATGCTTGACCCAGCC
TCATCTGTGGAGATGGTAAAAGTTTTAGAAGAAGATCCCATGGTTGGAGGTGTTGGGGGA
GATGTCCAGATTTTAAACAAGTACGATTCCTGGATCTCATTCCTCAGCAGTGTAAGATAT
TGGATGGCTTTTAATATAGAAAGGGCCTGTCAGTCTTATTTTGGGTGTGTTCAGTGCATT
AGTGGACCTCTGGGAATGTACAGAAACTCCTTGTTGCATGAGTTTGTGGAAGATTGGTAC
AATCAAGAATTTATGGGCAACCAATGTAGCTTTGGTGATGACAGGCATCTCACGAACCGG
GTGCTGAGCCTGGGCTATGCAACAAAATACACAGCTCGATCTAAGTGCCTTACTGAAACA
CCTATAGAATATCTCAGATGGCTAAACCAGCAGACCCGTTGGAGCAAGTCCTACTTCCGA
GAATGGCTGTACAATGCAATGTGGTTTCACAAACATCACTTGTGGATGACCTACGAAGCG
ATTATCACTGGATTCTTTCCTTTCTTTCTCATTGCCACAGTAATCCAGCTCTTCTACCGG
GGTAAAATTTGGAACATTCTCCTCTTCTTGTTAACTGTCCAGCTAGTAGGTCTCATAAAA
TCATCTTTTGCCAGCTGCCTTAGAGGAAATATCGTCATGGTCTTCATGTCTCTCTACTCA
GTGTTATACATGTCGAGTTTACTTCCCGCCAAGATGTTTGCAATTGCAACAATAAACAAA
GCTGGGTGGGGCACATCAGGAAGGAAAACCATTGTTGTTAATTTCATAGGACTCATTCCA
GTATCAGTTTGGTTTACAATCCTCCTGGGTGGTGTGATTTTCACCATTTATAAGGAGTCT
AAAAGGCCATTTTCAGAATCCAAACAGACAGTTCTAATTGTTGGAACGTTGCTCTATGCA
TGCTATTGGGTCATGCTTTTGACGCTGTATGTAGTTCTCATCAATAAGTGTGGCAGGCGG
AAGAAGGGACAACAATATGACATGGTGCTTGATGTATGA
>OriGene 5' read for NM_005328 unedited
CCATTCCCCCGCCCCGCTGCGCATTGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATAA
GCAGTTTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
CGAATTCGGCACGAGGAAGAGTTGAACCACCTAAGGCGGAGTTCAAACAAAGGCTGAAGC
AGGAAGGTTTGCTGGGGGAAATCAGTGGGGTCCCCTGAGCAGCGTGAAGTCATCAGGCCA
AGGTCGTCTCAAATTCATCTGATCTCTTATTACCTCAATTTTGGAAACTGCCCGCCACCG
ACCCTCCGGGACCACACAGACAGGCTGAGGACGACTTTATGACCAAGAGCTGAACAAGAT
GCATTGTGAGAGGTTTCTATGTATCCTGAGAATAATTGGAACCACACTCTTTGGAGTCTC
TCTCCTCCTTGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACGGATAA
TTACTATTTCTCTTTTGGACTGTATGGTGCCTTTTTGGCATCACACCTCATCATCCAAAG
CCTGTTTGCCTTTTTGGAGCACCGAAAAATGAAAAAATCCCTAGAAACCCCCATAAAGTT
GAACAAAACAGTTGCCCTTTGCATCGCTGCCTATCAAGAAGAGCCAGACTACTTAAGGAA
ATGTTTGCAATCTGTGAAAAGGCTAACCTACCCTGNGATTAAAGTTGTCATGGTCATAGA
TGGGAACTCAGCAGATGACCTTTACATGATGGACATCTTCAGTGAAGTCATGGGCAGAGA
CTAATCAGCCACTTATATCTGGAAGACAACTTCCCGAAAAGGGTCCCTGTGAGACAGATG
AGTCACATACAGCAAGCTCGCTACACGTAACGCTAATTGGTCTTGTCCTACACCAGTATC
TGCATCATGCTAAAATGTNNGTGGGACAAGAGCAGTCT
>OriGene 3' read for NM_005328 unedited
CCGGAAATACTATTACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCTTA
TCAAAAATATTTTATTTACAAAAAATAATTATACTATACATCCTATACTGGAAATACATT
GAATAATTGCTAAAATAAATACAGGCAATTAATTCAACTTTTTATAAGAATGAGAGAATT
TGACATTTGAATGTTATCAAAGCTTAACTTAGAACATAATTAGTTAAAAAGGCAAACTCA
AGTTTTCATCTCATTTATTGGCATGGTGTGTTTTGTTTTCCACAAGCTCTCCAATCAGTG
AGAGTCTTGAGTCTCAGATGTACCTTTTTGTACTCTGAAGGTAGGATGTGCAGCTTTTTC
TTAGGCAGGATAGATGTAACATAGATGACTGCCTAAAAAAGAGGCAGAAAGGCAGGAAGC
CCATTTTTTTTTTAACACTTCTCCATGTGTCTCTGCCTCACCATAATGCTGTTTATTCAA
TTATTCATATATTCCGATTGTGTTTTCAACACAGGTTTACACAGCACCAGCTACAAGGCA
AGGTCAATCGTGTAAAAAATAAAACTGGATAATGGAGGCCTTGCAGTAACTAAATTAGCC
AAGTTCAGAGTATGTGGTTCAACAATTTTTATATGTGCCCATCATTGATATACTTATTAA
ACCTTGATGTCACTTCTACTGGGGAGACATTTTTATTCAGGTCCACAACAGGAAATAAGA
AGTGATGATCTGTGTAATTCACAAAAACAAATTCCTCCTTTGTAAAGTAAAAGAGGAAAG
AAACTTGGTGGAGTCTTCTTGCCCAATTTCAAATTATATGGTTTTAAAAATTATCCCACT
TCACCCGGGTAGACCTTTTTACCTCCCTGGTAAAGGGAACG
Restriction Sites NotI-NotI     
ACCN NM_005328
Insert Size 4000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005328.1, NP_005319.1
RefSeq Size 3003 bp
RefSeq ORF 1659 bp
Locus ID 3037
Cytogenetics 8q24.13
Domains Glycos_transf_2
Protein Families Druggable Genome, Transmembrane
Gene Summary ' Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]'

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