HAS3 (NM_005329) Human Untagged Clone

CAT#: SC116827

HAS3 (untagged)-Human hyaluronan synthase 3 (HAS3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HAS3
• Synonyms: HA synthase 3; hyaluronan synthase 3; hyaluronic acid synthase 3; OTTHUMP00000174869
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005329, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGGTGCAGCTGACGACAGCCCTGCGTGTGGTGGGCACCAGCCTGTTTGCCCTGGCAGTGCTGGGTG
  GCATCCTGGCAGCCTATGTGACGGGCTACCAGTTCATCCACACGGAAAAGCACTACCTGTCCTTCGGCCT
  GTACGGCGCCATCCTGGGCCTGCACCTGCTCATTCAGAGCCTTTTTGCCTTCCTGGAGCACCGGCGCATG
  CGACGTGCCGGCCAGGCCCTGAAGCTGCCCTCCCCGCGGCGGGGCTCGGTGGCACTGTGCATTGCCGCAT
  ACCAGGAGGACCCTGACTACTTGCGCAAGTGCCTGCGCTCGGCCCAGCGCATCTCCTTCCCTGACCTCAA
  GGTGGTCATGGTGGTGGATGGCAACCGCCAGGAGGACGCCTACATGCTGGACATCTTCCACGAGGTGCTG
  GGCGGCACCGAGCAGGCCGGCTTCTTTGTGTGGCGCAGCAACTTCCATGAGGCAGGCGAGGGTGAGACGG
  AGGCCAGCCTGCAGGAGGGCATGGACCGTGTGCGGGATGTGGTGCGGGCCAGCACCTTCTCGTGCATCAT
  GCAGAAGTGGGGAGGCAAGCGCGAGGTCATGTACACGGCCTTCAAGGCCCTCGGCGATTCGGTGGACTAC
  ATCCAGGTGTGCGACTCTGACACTGTGCTGGATCCAGCCTGCACCATCGAGATGCTTCGAGTCCTGGAGG
  AGGATCCCCAAGTAGGGGGAGTCGGGGGAGATGTCCAGATCCTCAACAAGTACGACTCATGGATTTCCTT
  CCTGAGCAGCGTGCGGTACTGGATGGCCTTCAACGTGGAGCGGGCCTGCCAGTCCTACTTTGGCTGTGTG
  CAGTGTATTAGTGGGCCCTTGGGCATGTACCGCAACAGCCTCCTCCAGCAGTTCCTGGAGGACTGGTACC
  ATCAGAAGTTCCTAGGCAGCAAGTGCAGCTTCGGGGATGACCGGCACCTCACCAACCGAGTCCTGAGCCT
  TGGCTACCGAACTAAGTATACCGCGCGCTCCAAGTGCCTCACAGAGACCCCCACTAAGTACCTCCGGTGG
  CTCAACCAGCAAACCCGCTGGAGCAAGTCTTACTTCCGGGAGTGGCTCTACAACTCTCTGTGGTTCCATA
  AGCACCACCTCTGGATGACCTACGAGTCAGTGGTCACGGGTTTCTTCCCCTTCTTCCTCATTGCCACGGT
  TATACAGCTTTTCTACCGGGGCCGCATCTGGAACATTCTCCTCTTCCTGCTGACGGTGCAGCTGGTGGGC
  ATTATCAAGGCCACCTACGCCTGCTTCCTTCGGGGCAATGCAGAGATGATCTTCATGTCCCTCTACTCCC
  TCCTCTATATGTCCAGCCTTCTGCCGGCCAAGATCTTTGCCATTGCTACCATCAACAAATCTGGCTGGGG
  CACCTCTGGCCGAAAAACCATTGTGGTGAACTTCATTGGCCTCATTCCTGTGTCCATCTGGGTGGCAGTT
  CTCCTGGGAGGGCTGGCCTACACAGCTTATTGCCAGGACCTGTTCAGTGAGACAGAGCTAGCCTTCCTTG
  TCTCTGGGGCTATACTGTATGGCTGCTACTGGGTGGCCCTCCTCATGCTATATCTGGCCATCATCGCCCG
  GCGATGTGGGAAGAAGCCGGAGCAGTACAGCTTGGCTTTTGCTGAGGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005329 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCGCGGAGCGGAGCGGGA
  GGAGGGGCATGGAGCCGCCGCGGGCCTGCTGAGCTCCGGAGCGCGGCAGCCGGCGGCACG
  ATGCCGGTGCAGCTGACGACAGCCCTGCGTGTGGTGGGCACCAGCCTGTTTGCCCTGGCA
  GTGCTGGGTGGCATCCTGGCAGCCTATGTGACGGGCTACCAGTTCATCCACACGGAAAAG
  CACTACCTGTCCTTCGGCCTGTACGGCGCCATCCTGGGCCTGCACCTGCTCATTCAGAGC
  CTTTTTGCCTTCCTGGAGCACCGGCGCATGCGACGTGCCGGCCAGGCCCTGAAGCTGCCC
  TCCCCGCGGCGGGGCTCGGTGGCACTGTGCATTGCCGCGTACCAGGAGGACCCTGACTAC
  TTGCGCAAGTGCCTGCGCTCGGCCCAGCGCATCTCCTTCCCTGACCTCAAGGTGGTCATG
  GTGGTGGATGGCAACCGCCAGGAGGACGCCTACATGCTGGACATCTTCCACGAGGTGCTG
  GGCGGCACCGAGCAGGCCGGCTTCTTTGTGTGGCGCAGCAACTTCCATGAGGCANGCGAG
  GGTGAGACGGAGGCCAGCCTGCAGGAGGGCATGGACCGTGTGCGGGATGTGGTGCGGGCC
  AGCACCTTCTCGTGCATCATGCAGAAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005329 unedited
  TTGGACCGCGGCCGCAATTCTAGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TGCATATGGGAGAAATACTTTATTTTGACAAATAAGTGCAGTATAAAAAACTTGACCTGA
  AAATTAAAAAAAAAAAAAAAAAGCAGGGAAAAATAAATATGTTTACAAATTATTGGAGAA
  ACAATACAAAAGAGGATTTGAATTCTCAATGAGGAAAAACCAAAATGCGTCCAATTGTAT
  AAAGGCTCTTCCCTTGCAAGAAACGGGGATAAAGCTGAAAACCCAGTTTGGTTTTGCTGC
  TGAAAAATGTACAAAATAACTTAAAAAAACCAGTCGAGGGCAAACGTTGGGAGCCCACTA
  CCTGTACCTGCCTGTTTTTGATTTGAGGAAAGTTGACGACCACAGTGCAAGGCAAAGCTG
  ATGGTAGGACAGCCAGGAAAGCCTGGCATCCTACATTAATCTTCAAGGAAATGCTGAAGA
  AAAACAAGCCCTTAACGAAGTCTGAACTCTGCACTTGGCTGACCGGATTTCCTCCATTCT
  AATGCTCAAAATAGTTTAACTTCTTGGCCAGCAGAACGATTGCCTGGATCAGCTGCCTTA
  ACAAATGCCTGGTGGCTTCCAGCCTTCATGGGTTGGGTCTGATGCCAACCAGCAGCTTTA
  CAGAAACTTGGAGGCTGTGGAAGGGCGCATTGTTACCTCCCCCGGNAAACCCTTGAGTTT
  CCGGGANATAAAGAAACCCNCGGTTTGCCATTTGGGGGCAGAGNCACCTTGGGGCTTGGG
  GGAAAACTGNAACATAAAGTNGCCCTTTTGTGGCNTAACCCCACAACCAGTTCGACCGGT
  TTGGTGCCCTTATACAGCCACTACGGTGNNCAATGGAGCTATNNNTAACTAAGGCTCTTC
  CNTATTGCTGTCCTCCATGTAATCCAGATGTCNATTATGTGATGCCGTGACTATCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005329
• Insert Size: 4180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005329.2, NP_005320.2
• RefSeq Size: 4220 bp
• RefSeq ORF: 1662 bp
• Locus ID: 3038
• Cytogenetics: 16q22.1
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (1) contains a different 5' exon as compared to variant 3. Both variants 1 and 3 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.