CRKL (NM_005207) Human Untagged Clone

CAT#: SC116881

CRKL (untagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRKL
• Synonyms: v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116881 sequence for NM_005207 edited (data generated by NextGen Sequencing)
  ATGTCCTCCGCCAGGTTCGACTCCTCGGACCGCTCCGCCTGGTATATGGGGCCGGTGTCT
  CGCCAGGAGGCGCAGACCCGGCTCCAGGGCCAGCGCCACGGTATGTTCCTCGTCCGCGAT
  TCTTCCACCTGCCCTGGGGACTATGTGCTGTCGGTGTCCGAGAACTCGCGGGTCTCCCAC
  TACATCATCAACTCGCTGCCCAACCGCCGTTTTAAGATCGGGGACCAGGAATTTGACCAT
  TTGCCGGCCCTGCTGGAGTTTTACAAGATCCACTACCTGGACACCACCACCCTCATCGAG
  CCTGCGCCCAGGTATCCAAGCCCACCAATGGGATCTGTCTCAGCACCCAACCTGCCTACA
  GCAGAAGATAACCTGGAATATGTACGGACTCTGTATGATTTTCCTGGGAATGATGCCGAA
  GACCTGCCCTTTAAAAAGGGTGAGATCCTAGTGATAATAGAGAAGCCTGAAGAACAGTGG
  TGGAGTGCCCGGAACAAGGATGGCCGGGTTGGGATGATTCCTGTCCCTTATGTCGAAAAG
  CTTGTGAGATCCTCACCACACGGAAAGCATGGAAATAGGAATTCCAACAGTTATGGGATC
  CCAGAACCTGCTCATGCATACGCTCAACCTCAGACCACAACTCCTCTACCTGCAGTTTCC
  GGTTCTCCTGGGGCAGCAATCACCCCTTTGCCATCCACACAGAATGGACCTGTCTTTGCG
  AAAGCAATCCAGAAAAGAGTACCCTGTGCTTATGACAAGACTGCCTTGGCATTAGAGGTT
  GGTGACATCGTGAAAGTCACAAGGATGAATATAAATGGCCAGTGGGAAGGCGAAGTGAAC
  GGGCGCAAAGGGCTTTTCCCCTTTACGCACGTCAAAATCTTTGACCCTCAAAACCCAGAT
  GAAAACGAGTGA
  
  Clone variation with respect to NM_005207.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005207 unedited
  NTGTTAGTTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGCTGCCGCTT
  CTCCCGCGTCCGCCATTTTGTTGCTGTGGCTATTGGGAACAAGCTGGGCAAAAGCACCCC
  GGAGGCGCGACGCTCCTTCGAGTTCGGTGCCTCGTGTGACGGCGGGGGTCGGTGAAGACC
  CGTCGAGCTGCGGCGCCGGCGCGTTCCAGGCCGGGAGTCACTGGAGGCACCCCTGGGACG
  CCGAGCAGCCCGAGAACCCCGGGGTGGCCTCCGCTGCGGCTCGGGTTTGCCTGCCCCGAC
  CCCCCGGCTCTGCCGTGCATTCCCGGGCGGCTCTCTCCGTGTGGCGGCCCCGGAGCAGGC
  GGGCGGCGTCGGAGGATGCTGCGGGCCCGGAGCCGAGAGGAAAGTGCTGGCCCAGCCCTC
  TGAGCGCTCCTCGAGGTGTGCGAGAGGCCCTTCCTCGGCCCCAAAGCCGTCTGCCGGGCT
  AAGGCGTGCAGAGCAGGCGAGGACAGCCGCCGCCCCTACCGCCGCAGAGTCCCCGGTCCA
  ACACCATGTCCTCCGCCAGGTTCGACTCCTCGGACCGCTCCGCCTGGTATATGGGGCCGG
  TGTCTCGCCAGGAGGCGCAGACCCGGCTCCAGGGCCAGCGCCACGGTATGTTCCTCGTCC
  GCGATTCTTCCACCTGCCCTGNGGACTATGTGCTGTCGGTGTCCGAGAACTCGCGGGTCT
  CCCACTACATCATCAACTCGCTGCCCAACCGCCGTTTTAAGATCCGGGACCAGGAATTTG
  ACCATTTGCCCGGCCCTGCTGGAGTTTTACCAGATCCACTACCTGGACACCACCACCCTC
  ATCGAGCCTGCGCCCAGGTATCCAAGCCCACCATGGGGATCTGTCTCAGCACCCACCTGC
  CTACGNAGAAGATACCTGGAAATGTACGACTCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005207 unedited
  NNCCCCTTTTTANACCAGAATTCTCTCTTTCCTNAAGTTCACTTCCTGTTGTCCAAANAA
  AAGCAGATGGCAGCTAAGCCACTGCTTTGTGGAACAGCAAACATGCAGTATCTCGCCATG
  GCCTCAAGCTTTCTCTGGTGAACACAACAGAATCAGTCCCATGTACAAACGGGCAGCAAT
  GAGAAGGAAGACCCTTTCCATGTCAACTTCTCTCCACTTGTGTGCTTCCATCAGTATTTC
  CAGTTTATGATCCAAAAGAACTCTAAAATCGGCTACTCTTTGACAATACGACTATGATAC
  AAACATCAGGAGGCAGCCGCTGTGTGCATTCCAAATGTGTGTGCGACTGCCAGTCTGCAG
  AAAGCTGGACTTCGGCAATGCAGTGTGACTTGCCTATGAGAGAAAATGCTTTCCCACTTC
  AAAGGAGACTAGGACAGGCAGAACAACAAAGCAGCAGGAAACAGGGCAACAGCAATCACT
  CGTTTTCATCTGGGTTTTGAGGGTCAAAGATTTTGACGTGCGTAAAGGGGAAAAGCCCTT
  TGCGCCCGTTCACTTCGCCTTCCCACTGGCCATTTATATTCATCCTTGTGACTTTCACGA
  TGTCACCCACCTCTAATGCCCAAGCAGTCTTGTCATAAGCACAGGGTACTCCTTTCTGGA
  TTGCTTTCGCAAAGACAGGTCCATTCTGTGTGGGATGCAAAAGGGGTGAATTGCTGCCCC
  AGGAGAACCCGAAAACTGCAGGGAAAAGGAGTTTGTGGCTGAAGGTGAACCGATTCCTTG
  AACCGGGTCTGGGGTCCCATTACCGGTGGGATTCCTTTTTCCCTGCCTTCCCGGGGGGGG
  AGGAACCCCCAAACCTTTCCGCCTTAAGGGGACAGGGATTATCCCCACCCCGGCTTCCTG
  TGTCCGGGACCTTCCCATTGTTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005207
• Insert Size: 1910 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005207.2, NP_005198.1
• RefSeq Size: 5235 bp
• RefSeq ORF: 912 bp
• Locus ID: 1399
• Cytogenetics: 22q11.21
• Domains: SH2, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
• Gene Summary: 'This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]'