ADA (NM_000022) Human Untagged Clone

CAT#: SC116886

ADA (untagged)-Human adenosine deaminase (ADA)


  "NM_000022" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ADA
Synonyms ADA1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116886 sequence for NM_000022 edited (data generated by NextGen Sequencing)
ATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCACCTAGACGGA
TCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGCCCTCCCAGCT
AACACAGCAGAGGGGCTGCTGAACGTCATTGGCATGGACAAGCCGCTCACCCTTCCAGAC
TTCCTGGCCAAGTTTGACTACTACATGCCTGCTATCGCGGGCTGCCGGGAGGCTATCAAA
AGGATCGCCTATGAGTTTGTAGAGATGAAGGCCAAAGAGGGCGTGGTGTATGTGGAGGTG
CGGTACAGTCCGCACCTGCTGGCCAACTCCAAAGTGGAGCCAATCCCCTGGAACCAGGCT
GAAGGGGACCTCACCCCAGACGAGGTGGTGGCCCTAGTGGGCCAGGGCCTGCAGGAGGGG
GAGCGAGACTTCGGGGTCAAGGCCCGGTCCATCCTGTGCTGCATGCGCCACCAGCCCAAC
TGGTCCCCCAAGGTGGTGGAGCTGTGTAAGAAGTACCAGCAGCAGACCGTGGTGGCCATT
GACCTGGCTGGAGATGAGACCATCCCAGGAAGCAGCCTCTTGCCTGGACATGTCCAGGCC
TACCAGGAGGCTGTGAAGAGCGGCATTCACCGTACTGTCCACGCCGGGGAGGTGGGCTCG
GCCGAAGTAGTAAAAGAGGCTGTGGACATACTCAAGACAGAGCGGCTGGGACACGGCTAC
CACACCCTGGAAGACCAGGCCCTTTATAACAGGCTGCGGCAGGAAAACATGCACTTCGAG
ATCTGCCCCTGGTCCAGCTACCTCACTGGTGCCTGGAAGCCGGACACGGAGCATGCAGTC
ATTCGGCTCAAAAATGACCAGGCTAACTACTCGCTCAACACAGATGACCCGCTCATCTTC
AAGTCCACCCTGGACACTGATTACCAGATGACCAAACGGGACATGGGCTTTACTGAAGAG
GAGTTTAAAAGGCTGAACATCAATGCGGCCAAATCTAGTTTCCTCCCAGAAGATGAAAAG
AGGGAGCTTCTCGACCTGCTCTATAAAGCCTATGGGATGCCACCTTCAGCCTCTGCAGGG
CAGAACCTCTGA

Clone variation with respect to NM_000022.2
534 a=>g
>OriGene 5' read for NM_000022 unedited
GCAAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAAAGCCGAG
CGGCCACCGAGCCGGCAGAGACCCACCGAGCGGCGGCGGAGGGAGCAGCGCCGGGGCGCA
CGAGGGCACCATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCA
CCTAGACGGATCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGC
CCTCCCAGCTAACACAGCAGAGGGGCTGCTGAACGTCATTGGCATGGACAAGCCGCTCAC
CCTTCCAGACTTCCTGGCCAAGTTTGACTACTACATGCCTGCTATCGCGGGCTGCCGGGA
GGCTATCAAAAGGATCGCCTATGAGTTTGTAGAGATGAAGGCCAAAGAGGGCGTGGTGAT
TTGNGAAGTGGGCCGTACAGTCCGCACCTGCTGGCCAACTCCAAAGTGGAGCCAATCCCC
TGGAACCAGGCTGAAGGGGACCTCACCCCAGACAGGNTGGGTGGCCCTAGTGGGCCAGGG
GCTGCAGGAGGNGGAGCGAGACTTCGGGGTCAAGGCCCGGTCCATCCTGTGCTGCATGCG
CCACCAGCCCAACTGGTCCCCAAGGNTGGGTGGAGCTGTGTAAAGAAGTACCAGCAGCAG
ACCGGTGGTGGCCATTGACCTGGCTGGGAGATGAGACCATCCCCAGAAGCAGCCTCTTGC
CTGGACATGTCCAGCCTACCAGNAGGCTTGTGAAGAAGCGCATTCACCGTACTGCCACCG
GGGGAAGGGGGGGCTCGGGCCGAGTGTAAAAGAAGCTGGGGACATACTCAAGACGAGCGG
TTGGGACACGCTACACACCCTGGAAGACAGGCCCTTATACAGCTGCGGCAGGAAACTGCC
TTTCAGATTTGCCCTGGCCACTACTACTTGGGCCTGAAACCGACCCGGACAGGATATTTG
GGTA
>OriGene 3' read for NM_000022 unedited
GGGGGTGGGCCCCACNNCACCTTTTCCNNNNNGNNTTACTGTGNACCCGGCCGCTTTTNA
NATTTTTTTTTTTTGCATGCCACCAGCCTGGGCTTCTTTATTGAGCACCAGNATTTCAGT
ACAAGTTGCCACAGAAGGAGGGTTTCAGATTCAACCATGCCCATGTGCAAGGGCGCTGGT
CCCTGGCCAGGGCACATAATCAGAGAAGTGACGCGGCCATGCCGAGGTATACGTGTGTGC
AGAAATGGACACATAGGGTTCAGGAGCATCAGTAACTGACTATTGAGATCATGGTCTTCT
TGGAAGGAATAAATGTAAAAATGTTGCTCAGCCCCACAGAGTTGGGGTGACTCCACAGGG
TGAAGGCTTGGAGGAGTGGCGTCTTCAGAGGTTCTGCCCTGCAGAGGCTGAAGGTGGCAT
CCCATAGGCTTTATAGAGCAGGTCGAGAAGCTCCCTCTTTTCATCTTCTGGGAGGAAACT
AGATTTGGCCGCATTGATGTTCAGCCTTTTAAACTCCTCTTCAGTAAAGCCCATGTCCCG
TTTGGTCATCTGGTAATCAGTGTCCAGGGTGGACTTGAAGATGAGCGGGTCATCTGTGTT
GAGCGAGTAGTTAGCCTGGTCATTTTTGAGCCGAATGACTGCATGCTCCGTGTCCGGCTT
CCANGCACCAGTGAGGTAGCTGGACCAGGGGCAGATCTCGAAGTGCATGTTTTCCTGCCG
CAGCCTGTTATAAAAGGCCTGGTCTTCCAGGGTGTGGTAGCCGTGTCCCAGCCGCTCTGT
CTTGAGTATGTCCACAGCCTTTTTTACTACTTTCGNCGAGCCCACCTCCCCGCCGTGGAC
AGTACCGGGAATGCCGCTCTTACAGGCTCCTGGTAGGCCTGACATGTTCAGGCAAGAGCT
GCTTCCTGGGAGGCCTCATTTCCAGCCAGTCATGGCCACACGGCTGCTG
Restriction Sites NotI-NotI     
ACCN NM_000022
Insert Size 2800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000022.2, NP_000013.2
RefSeq Size 1566 bp
RefSeq ORF 1092 bp
Locus ID 100
Cytogenetics 20q13.12
Domains A_deaminase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Primary immunodeficiency, Purine metabolism
Gene Summary 'This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia. [provided by RefSeq, Sep 2019]'

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