ADA (NM_000022) Human Untagged Clone

CAT#: SC116886

ADA (untagged)-Human adenosine deaminase (ADA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADA
• Synonyms: ADA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116886 sequence for NM_000022 edited (data generated by NextGen Sequencing)
  ATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCACCTAGACGGA
  TCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGCCCTCCCAGCT
  AACACAGCAGAGGGGCTGCTGAACGTCATTGGCATGGACAAGCCGCTCACCCTTCCAGAC
  TTCCTGGCCAAGTTTGACTACTACATGCCTGCTATCGCGGGCTGCCGGGAGGCTATCAAA
  AGGATCGCCTATGAGTTTGTAGAGATGAAGGCCAAAGAGGGCGTGGTGTATGTGGAGGTG
  CGGTACAGTCCGCACCTGCTGGCCAACTCCAAAGTGGAGCCAATCCCCTGGAACCAGGCT
  GAAGGGGACCTCACCCCAGACGAGGTGGTGGCCCTAGTGGGCCAGGGCCTGCAGGAGGGG
  GAGCGAGACTTCGGGGTCAAGGCCCGGTCCATCCTGTGCTGCATGCGCCACCAGCCCAAC
  TGGTCCCCCAAGGTGGTGGAGCTGTGTAAGAAGTACCAGCAGCAGACCGTGGTGGCCATT
  GACCTGGCTGGAGATGAGACCATCCCAGGAAGCAGCCTCTTGCCTGGACATGTCCAGGCC
  TACCAGGAGGCTGTGAAGAGCGGCATTCACCGTACTGTCCACGCCGGGGAGGTGGGCTCG
  GCCGAAGTAGTAAAAGAGGCTGTGGACATACTCAAGACAGAGCGGCTGGGACACGGCTAC
  CACACCCTGGAAGACCAGGCCCTTTATAACAGGCTGCGGCAGGAAAACATGCACTTCGAG
  ATCTGCCCCTGGTCCAGCTACCTCACTGGTGCCTGGAAGCCGGACACGGAGCATGCAGTC
  ATTCGGCTCAAAAATGACCAGGCTAACTACTCGCTCAACACAGATGACCCGCTCATCTTC
  AAGTCCACCCTGGACACTGATTACCAGATGACCAAACGGGACATGGGCTTTACTGAAGAG
  GAGTTTAAAAGGCTGAACATCAATGCGGCCAAATCTAGTTTCCTCCCAGAAGATGAAAAG
  AGGGAGCTTCTCGACCTGCTCTATAAAGCCTATGGGATGCCACCTTCAGCCTCTGCAGGG
  CAGAACCTCTGA
  
  Clone variation with respect to NM_000022.2
  534 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000022 unedited
  GCAAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAAAGCCGAG
  CGGCCACCGAGCCGGCAGAGACCCACCGAGCGGCGGCGGAGGGAGCAGCGCCGGGGCGCA
  CGAGGGCACCATGGCCCAGACGCCCGCCTTCGACAAGCCCAAAGTAGAACTGCATGTCCA
  CCTAGACGGATCCATCAAGCCTGAAACCATCTTATACTATGGCAGGAGGAGAGGGATCGC
  CCTCCCAGCTAACACAGCAGAGGGGCTGCTGAACGTCATTGGCATGGACAAGCCGCTCAC
  CCTTCCAGACTTCCTGGCCAAGTTTGACTACTACATGCCTGCTATCGCGGGCTGCCGGGA
  GGCTATCAAAAGGATCGCCTATGAGTTTGTAGAGATGAAGGCCAAAGAGGGCGTGGTGAT
  TTGNGAAGTGGGCCGTACAGTCCGCACCTGCTGGCCAACTCCAAAGTGGAGCCAATCCCC
  TGGAACCAGGCTGAAGGGGACCTCACCCCAGACAGGNTGGGTGGCCCTAGTGGGCCAGGG
  GCTGCAGGAGGNGGAGCGAGACTTCGGGGTCAAGGCCCGGTCCATCCTGTGCTGCATGCG
  CCACCAGCCCAACTGGTCCCCAAGGNTGGGTGGAGCTGTGTAAAGAAGTACCAGCAGCAG
  ACCGGTGGTGGCCATTGACCTGGCTGGGAGATGAGACCATCCCCAGAAGCAGCCTCTTGC
  CTGGACATGTCCAGCCTACCAGNAGGCTTGTGAAGAAGCGCATTCACCGTACTGCCACCG
  GGGGAAGGGGGGGCTCGGGCCGAGTGTAAAAGAAGCTGGGGACATACTCAAGACGAGCGG
  TTGGGACACGCTACACACCCTGGAAGACAGGCCCTTATACAGCTGCGGCAGGAAACTGCC
  TTTCAGATTTGCCCTGGCCACTACTACTTGGGCCTGAAACCGACCCGGACAGGATATTTG
  GGTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000022 unedited
  GGGGGTGGGCCCCACNNCACCTTTTCCNNNNNGNNTTACTGTGNACCCGGCCGCTTTTNA
  NATTTTTTTTTTTTGCATGCCACCAGCCTGGGCTTCTTTATTGAGCACCAGNATTTCAGT
  ACAAGTTGCCACAGAAGGAGGGTTTCAGATTCAACCATGCCCATGTGCAAGGGCGCTGGT
  CCCTGGCCAGGGCACATAATCAGAGAAGTGACGCGGCCATGCCGAGGTATACGTGTGTGC
  AGAAATGGACACATAGGGTTCAGGAGCATCAGTAACTGACTATTGAGATCATGGTCTTCT
  TGGAAGGAATAAATGTAAAAATGTTGCTCAGCCCCACAGAGTTGGGGTGACTCCACAGGG
  TGAAGGCTTGGAGGAGTGGCGTCTTCAGAGGTTCTGCCCTGCAGAGGCTGAAGGTGGCAT
  CCCATAGGCTTTATAGAGCAGGTCGAGAAGCTCCCTCTTTTCATCTTCTGGGAGGAAACT
  AGATTTGGCCGCATTGATGTTCAGCCTTTTAAACTCCTCTTCAGTAAAGCCCATGTCCCG
  TTTGGTCATCTGGTAATCAGTGTCCAGGGTGGACTTGAAGATGAGCGGGTCATCTGTGTT
  GAGCGAGTAGTTAGCCTGGTCATTTTTGAGCCGAATGACTGCATGCTCCGTGTCCGGCTT
  CCANGCACCAGTGAGGTAGCTGGACCAGGGGCAGATCTCGAAGTGCATGTTTTCCTGCCG
  CAGCCTGTTATAAAAGGCCTGGTCTTCCAGGGTGTGGTAGCCGTGTCCCAGCCGCTCTGT
  CTTGAGTATGTCCACAGCCTTTTTTACTACTTTCGNCGAGCCCACCTCCCCGCCGTGGAC
  AGTACCGGGAATGCCGCTCTTACAGGCTCCTGGTAGGCCTGACATGTTCAGGCAAGAGCT
  GCTTCCTGGGAGGCCTCATTTCCAGCCAGTCATGGCCACACGGCTGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000022
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000022.2, NP_000013.2
• RefSeq Size: 1566 bp
• RefSeq ORF: 1092 bp
• Locus ID: 100
• Cytogenetics: 20q13.12
• Domains: A_deaminase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary immunodeficiency, Purine metabolism
• Gene Summary: 'This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia. [provided by RefSeq, Sep 2019]'