BRD2 (NM_005104) Human Untagged Clone

CAT#: SC116928

BRD2 (untagged)-Human bromodomain containing 2 (BRD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRD2
• Synonyms: BRD2-IT1; D6S113E; FSH; FSRG1; NAT; O27.1.1; RING3; RNF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005104, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCAAAACGTGACTCCCCACAATAAGCTCCCTGGGGAAGGGAATGCAGGGTTGCTGGGGCTGGGCC
  CAGAAGCAGCAGCACCAGGGAAGAGGATTCGAAAACCCTCTCTCTTGTATGAGGGCTTTGAGAGCCCCAC
  AATGGCTTCGGTGCCTGCTTTGCAACTTACCCCTGCCAACCCACCACCCCCGGAGGTGTCCAATCCCAAA
  AAGCCAGGACGAGTTACCAACCAGCTGCAATACCTACACAAGGTAGTGATGAAGGCTCTGTGGAAACATC
  AGTTCGCATGGCCATTCCGGCAGCCTGTGGATGCTGTCAAACTGGGTCTACCGGATTATCACAAAATTAT
  AAAACAGCCTATGGACATGGGTACTATTAAGAGGAGACTTGAAAACAATTATTATTGGGCTGCTTCAGAG
  TGTATGCAAGATTTTAATACCATGTTCACCAACTGTTACATTTACAACAAGCCCACTGATGATATTGTCC
  TAATGGCACAAACGCTGGAAAAGATATTCCTACAGAAGGTTGCATCAATGCCACAAGAAGAACAAGAGCT
  GGTAGTGACCATCCCTAAGAACAGCCACAAGAAGGGGGCCAAGTTGGCAGCGCTCCAGGGCAGTGTTACC
  AGTGCCCATCAGGTGCCTGCCGTCTCTTCTGTGTCACACACAGCCCTGTATACTCCTCCACCTGAGATAC
  CTACCACTGTCCTCAACATTCCCCACCCATCAGTCATTTCCTCTCCACTTCTCAAGTCCTTGCACTCTGC
  TGGACCCCCGCTCCTTGCTGTTACTGCAGCTCCTCCAGCCCAGCCCCTTGCCAAGAAAAAAGGCGTAAAG
  CGGAAAGCAGATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCTCCAGCTAGCCCTCCTG
  GGAGTCTTGAGCCTAAGGCAGCACGGCTTCCCCCTATGCGTAGAGAGAGTGGTCGCCCCATCAAGCCCCC
  ACGCAAAGACTTGCCTGACTCTCAGCAACAACACCAGAGCTCTAAGAAAGGAAAGCTTTCAGAACAGTTA
  AAACATTGCAATGGCATTTTGAAGGAGTTACTCTCTAAGAAGCATGCTGCCTATGCTTGGCCTTTCTATA
  AACCAGTGGATGCTTCTGCACTTGGCCTGCATGACTACCATGACATCATTAAGCACCCCATGGACCTCAG
  CACTGTCAAGCGGAAGATGGAGAACCGTGATTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTT
  ATGTTCTCCAACTGCTATAAGTACAATCCCCCAGATCACGATGTTGTGGCAATGGCACGAAAGCTACAGG
  ATGTATTTGAGTTCCGTTATGCCAAGATGCCAGATGAACCACTAGAACCAGGGCCTTTACCAGTCTCTAC
  TGCCATGCCCCCTGGCTTGGCCAAATCGTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGCTCC
  TCTGAGGAAGAGGAGGAGGAAGATGAGGAGGACGAGGAGGAAGAAGAGAGTGAAAGCTCAGACTCAGAGG
  AAGAAAGGGCTCATCGCTTAGCAGAACTACAGGAACAGCTTCGGGCAGTACATGAACAACTGGCTGCTCT
  GTCCCAGGGTCCAATATCCAAGCCCAAGAGGAAAAGAGAGAAAAAAGAGAAAAAGAAGAAACGGAAGGCA
  GAGAAGCATCGAGGCCGAGCTGGGGCCGATGAAGATGACAAGGGGCCTAGGGCACCCCGCCCACCTCAAC
  CTAAGAAGTCCAAGAAAGCAAGTGGCAGTGGGGGTGGCAGTGCTGCTTTAGGCCCTTCTGGCTTTGGACC
  TTCTGGAGGAAGTGGCACCAAGCTCCCCAAAAAGGCCACAAAGACAGCCCCACCTGCCCTGCCTACAGGT
  TATGATTCAGAGGAGGAGGAAGAGAGCAGGCCCATGAGTTACGATGAGAAGCGGCAGCTGAGCCTGGACA
  TCAACAAATTACCTGGGGAGAAGCTGGGCCGAGTTGTGCATATAATCCAAGCCAGGGAGCCCTCTTTACG
  TGATTCAAACCCAGAAGAGATTGAGATTGATTTTGAAACACTCAAGCCATCCACACTTAGAGAGCTTGAG
  CGCTATGTCCTTTCCTGCCTACGTAAGAAACCCCGGAAGCCCTACACCATTAAGAAGCCTGTGGGAAAGA
  CAAAGGAGGAACTGGCTTTGGAGAAAAAGCGGGAATTAGAAAAGCGGTTACAAGATGTCAGCGGACAGCT
  CAATTCTACTAAAAAGCCCCCCAAGAAAGCGAATGAGAAAACAGAGTCATCCTCTGCACAGCAAGTAGCA
  GTGTCACGCCTTAGCGCTTCCAGCTCCAGCTCAGATTCCAGCTCCTCCTCTTCCTCGTCGTCGTCTTCAG
  ACACCAGTGATTCAGACTCAGGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005104
• Insert Size: 3520 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005104.3, NP_005095.1
• RefSeq Size: 4907 bp
• RefSeq ORF: 2406 bp
• Locus ID: 6046
• Cytogenetics: 6p21.32
• Domains: BROMO
• Protein Families: Protein Kinase
• Gene Summary: 'This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (1) and variant 2 encode the same predominant isoform (1).