TLE3 (NM_005078) Human Untagged Clone

CAT#: SC116972

TLE3 (untagged)-Human transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (TLE3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005078, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTG
  AGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA
  CGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC
  TTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGC
  CTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGAC
  GGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCC
  CCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCT
  CCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCA
  TGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCC
  AGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACA
  GCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCC
  CGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAA
  AAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACC
  TTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCC
  AACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATA
  GGTATAATGGCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGA
  TGAGCCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGCCTCCACAACATCCCACC
  CCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCT
  GTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAG
  GAAAACCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGC
  CCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGT
  GCCGTGACCATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACA
  TCAGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTC
  CTGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGAC
  CTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCA
  TTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCACAA
  CCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGACATCTCCCATGATGGC
  ACCAAACTGTGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTAC
  AGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGG
  CATGGAGAGCAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGAG
  AGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACTGGGAAAGATAACCTTC
  TCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTG
  TGACATTTCAGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTTTATGAGGTC
  ATCTACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005078 unedited
  CCATTTGTAAACGACTTATATAGGCGGCCGCGCAATTCGCACCAGTCCACGCTCTCGGAA
  TCACGACCCCTCCCTGCCATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGG
  CAGCCGGGATTTAAATTCACGGTGGCTGAGTCTTGTGACAGGATCAAAGACGAATTCCAG
  TTCCTGCAAGCTCAGTATCACAGCCTCATAGTGGAGTACGACAAGCTGGCAAACGAGAAG
  ACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGCTTGAACATTGAA
  ATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGCCT
  TTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTC
  ACCATGACGGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCC
  CATGCCACACACGGCCCCCCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCA
  GGAATCCCCCCAGTGACAGGGAGCAGCTCCGGGCTGCTGGCACTGGGCGCCCTGNGCAGC
  CAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCATGAACTCGATCACAGAGAGAGA
  GAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCCAGTGAGAAGCAC
  CGGNGCTCTGCGGACTACAGCATGGAAGCCAAGAAAGCGAAGGCNGAGGAGAAGGACAGC
  TTGAGCCGATACGACAGTGATGGGGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAAA
  TGAAGACCCGCACGCCCCGGGTCAGCCCGGCACACTCCCTCCCTGAAATGGGCTGGNACA
  GGCCNGTAGCCTGAANAAAAGATGCCCCACAGCCTGGCTCGNTGGNNCTCTTCAGTAGCA
  CACCTTTCTNNCAGACAAAGACCTGGTCATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005078 unedited
  GGACAACTTATGGACGCGGCCGCATTCTTAAGATCGATTTTTTTTTTTTTTTTTTTTTTT
  TTTGGAATTTCCGGGGGGAAGGGGCCTCCTTTCCCATTGTTAATTCCCGAATTAGAAGTC
  GGTTTTTAACATTTTTAAAAAATTTTGGGCCCTGAAAAATCGGAAAAAAAAAACCCCCCC
  ATCCCCAAGAAAAAATTTGGGGTCCATTCCAGCACCCCCAACCCAAGGGGAGGGACAGGG
  CAAAAAAAAAGGGAAAAACAACCAAAACCTCTTGACCGGGTTTGGGGGGAAACCGAACCC
  TTAACCCTCCAATAAATTTTTTTGTACCACCTGCCACCATTGTTAAGGGGGAGGAGGAGA
  ACCAAAACCCTTCCAAGTCTGGGAAACACATAATTCCAGGCCCAGAATTCCAAACTGGGA
  CGGGGAACGTGCCCTTTAAGCCGGCCGAAGCGAACCCCTGAACCCTGGGTTGCCTGGGGC
  CAATCCTCCGCCATCCTGGGGGCCCCTCCCCTGGGGGTCTCCCTGTAAAACCCAATTCGG
  TTTTTCCCAAATTTTGACAGCCCTGCTGAAGTTCTGGTTAATTAAATAACCTCTTAAACG
  GGGGCCTTCTTGTAACCAAAGCCGGTTACAAGGAATTGGTAATCCGCTGAAATGTCCAAC
  TCAGAACGAACAAGGATTCTTAAAACTGGATATATGCTGCTCCATAAAGCGTCTTCCAGC
  CGTTGAAAAGGTATCTTTTCCATTGCTCACAAACCACTTGCCGCATTAGGCGAACTGTGA
  GGAAAGCACCCCACTTTTCTGCAGGTCCAACTGGGACTTGTCAGGCCTGGTGTGGGGGCA
  CAACCTCCAAGTTGGTGGTTTTCATGCCCAAAGGCAGCCATTCCCAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005078
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005078.1, NP_005069.1
• RefSeq Size: 5760 bp
• RefSeq ORF: 5760 bp
• Locus ID: 7090
• Cytogenetics: 15q23
• Domains: WD40, TLE_N
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.