POLR2K (NM_005034) Human Untagged Clone

CAT#: SC116985

POLR2K (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa (POLR2K)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2K
• Synonyms: ABC10-alpha; hRPB7.0; hsRPB10a; RPABC4; RPB7.0; RPB10alpha; RPB12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005034 edited
  ATGGACACCCAGAAGGACGTTCAACCTCCAAAGCAGCAACCAATGATATATATCTGTGGA
  GAGTGTCACACAGAAAATGAAATAAAATCTAGGGATCCAATCAKATGCAGAGAATGTGGA
  TACAGAATAATGTACAAGAAAAGGACTAAAAGATTGGTCGTTTTTGATGCTCGATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005034 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGTTGTATTTGGAAAC
  GCGGAGTGAGTTTTTCCGTGCTGTGTAGGGGCTAACAATGGACACCCAGAAGGACGTTCA
  ACCTCCAAAGCAGCAACCAATGATATATATCTGTGGAGAGTGTCACACAGAAAATGAAAT
  AAAATCTAGGGATCCAATCAGATGCAGAGAATGTGGATACAGAATAATGTACAAGAAAAG
  GACTAAAAGATTGGTCGTTTTTGATGCTCGATGAATGCTGGGAATTCAGAGGAATGTCTT
  CACTTATACTTGGATTTGCTCTCTTCCCATTTCTGATTGTTGTATAGCTTTCGATTTTGC
  TTACAGTAGTTCCCCCTTATCTTCGGGAGATACATTCCAAGGCCCCCAGTGAACTCCTGA
  AACCTCAAACAGTACCAAACCTTTATACACTGTTTTTTCCATATATATATACCTATGATA
  AAGTATAATGTATAAATTAAGCATAGCAAGAGATTAATAATAATGTAATAGAACAATGAT
  AACATACTATAATAAAAGTTATGTGAATGTGGTTGGTCTCTCTTGCTNTCAAAATATCTT
  CTTGTACAGTACTCACCTATTTTAGAATGTGGTTGACTACAGGTAACCAAAACCACAGAA
  AGGGAAACTTTGGATGAGGNGGGCACTACTGTACTTANGAATACANCTATATACATATGA
  TTTTATTTTTAAGACATATTATANTTGGGTATCTACTAATATTTTGNATAAAGCAATTTT
  TGNTCCATTACGNGACTTTTNTGNNTTATNGATATGTAATTAACACACNNATAAGGNTAA
  AGTTGCTCCCCCAACACACTTTTAATCAAACCTAAATCATCTGCAGTCCTTGTAAAAATT
  GCAGTTTCTAAACCCTCTGAAGTTTGATAANTAATTNATGCCAACAAAAAAAAAAAAAAC
  TGACTTTGATGGCGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005034 unedited
  ATGTACCGCGGAACGCTTTTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTGCAATAAA
  TTTATTTAATCAGAACTTCAGAGGTTCTAGAAACCTGCATTTTTAACAAGGACTGCAGAT
  GATTCTAGGTTTTGATTAAAAGTGTGGTTTGGGGAAGCAACTTTACCCTTTATTGTGTGT
  TAAATTACATATACAATAAAACAAAAAGTCACGTAATGGAACAAAAAATTGCTTTATACA
  AAATATTAGTAGATACCCAAATATAATATGGCCTTAAAAATAAAATCATATGTATATAGT
  TGCATTCCTAAGTACAGTAGTGCCCCCCTCATCCAAAGTTTCCCTTTCTGTGGTTTTGGT
  TACCTGCAGTCAACCCACATTCTAAAATAGGCGAGCACTGTACAAGAAGATATTCTGAAA
  GCCAGAGAGACCAACTACATTCACATCAACCTTTTATTATCGCCCGCCATCATTGCTCCT
  ATTACATCTTCATCCAACCCTTTGCTATGCCTAACCCCTTCCCCCCACCCTCTCATCGCC
  TCATCTCTCCGGCCTCATCACGCTTCCCGTCTCGTCTTCGCCCACTCGTCTTTTCATCTC
  CTCCCCCCTCTTTTACTCCCTTTCACCCCCACTCTGCCCCACTTCCCGCACTTCCCATCC
  CCCTTTATCCCTCCGCCACCACCTTCAACCCCTCCTCCCCTTCTCCCCCCACCTTCTCCT
  CCTTTCTCCCCCTCTCCCCCATTTTACTCCTCCAACACTCCTTCCTCCCCCCTCTCTCCT
  TCTCTCACCCCTCCACCTTTCTTTCCGCCTATCACCCCCTCCTCTTCCACTCCTTTCCTT
  TCTTCCGCTATCTTCTCTATCTCTCCCCTCCCTCCCTCCACCTCTCTCTACCACCCTCCC
  CTCACCATTCCCTATATCTTACTATCTTCCTGTCACATTTCTTAGATCTCTCACACTCTT
  GCTCCCTCGTTCTCATTCCCTCCTCCTCCTCACTCCCTTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005034
• Insert Size: 980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005034.3, NP_005025.1
• RefSeq Size: 971 bp
• RefSeq ORF: 177 bp
• Locus ID: 5440
• Cytogenetics: 8q22.2
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008]'