MMP9 (NM_004994) Human Untagged Clone

CAT#: SC116989

MMP9 (untagged)-Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMP9
• Synonyms: CLG4B; GELB; MANDP2; MMP-9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004994 edited
  GAATTCGGCACGAGGATGAGCCTCTGGCAGCCCCTGGTCCTGGTGCTCCTGGTGCTGGGC
  TGCTGCTTTGCTGCCCCCAGACAGCGCCAGTCCACCCTTGTGCTCTTCCCTGGAGACCTG
  AGAACCAATCTCACCGACAGGCAGCTGGCAGAGGAATACCTGTACCGCTATGGTTACACT
  CGGGTGGCAGAGATGCGTGGAGAGTCGAAATCTCTGGGGCCTGCGCTGCTGCTTCTCCAG
  AAGCAACTGTCCCTGCCCGAGACCGGTGAGCTGGATAGCGCCACGCTGAAGGCCATGCGA
  ACCCCACGGTGCGGGGTCCCAGACCTGGGCAGATTCCAAACCTTTGAGGGCGACCTCAAG
  TGGCACCACCACAACATCACCTATTGGATCCAAAACTACTCGGAAGACTTGCCGCGGGCG
  GTGATTGACGACGCCTTTGCCCGCGCCTTCGCACTGTGGAGCGCGGTGACGCCGCTCACC
  TTCACTCGCGTGTACAGCCGGGACGCAGACATCGTCATCCAGTTTGGTGTCGCGGAGCAC
  GGAGACGGGTATCCCTTCGACGGGAAGGACGGGCTCCTGGCACACGCCTTTCCTCCTGGC
  CCCGGCATTCAGGGAGACGCCCATTTCGACGATGACGAGTTGTGGTCCCTGGGCAAGGGC
  GTCGTGGTTCCAACTCGGTTTGGAAACGCAGATGGCGCGGCCTGCCACTTCCCCTTCATC
  TTCGAGGGCCGCTCCTACTCTGCCTGCACCACCGACGGTCGCTCCGACGGCTTGCCCTGG
  TGCAGTACCACGGCCAACTACGACACCGACGACCGGTTTGGCTTCTGCCCCAGCGAGAGA
  CTCTACACCCGGGACGGCAATGCTGATGGGAAACCCTGCCAGTTTCCATTCATCTTCCAA
  GGCCAATCCTACTCCGCCTGCACCACGGACGGTCGCTCCGACGGCTACCGCTGGTGCGCC
  ACCACCGCCAACTACGACCGGGACAAGCTCTTCGGCTTCTGCCCGACCCGAGCTGACTCG
  ACGGTGATGGGGGGCAACTCGGCGGGGGAGCTGTGCGTCTTCCCCTTCACTTTCCTGGGT
  AAGGAGTACTCGACCTGTACCAGCGAGGGCCGCGGAGATGGGCGCCTCTGGTGCGCTACC
  ACCTCGAACTTTGACAGCGACAAGAAGTGGGGCTTCTGCCCGGACCAAGGATACAGTTTG
  TTCCTCGTGGCGGCGCATGAGTTCGGCCACGCGCTGGGCTTAGATCATTCCTCAGTGCCG
  GAGGCGCTCATGTACCCTATGTACCGCTTCACTGAGGGGCCCCCCTTGCATAAGGACGAC
  GTGAATGGCATCCGGCACCTCTATGGTCCTCGCCCTGAACCTGAGCCACGGCCTCCAACC
  ACCACCACACCGCAGCCCACGGCTCCCCCGACGGTCTGCCCCACCGGACCCCCCACTGTC
  CACCCCTCAGAGCGCCCCACAGCTGGCCCCACAGGTCCCCCCTCAGCTGGCCCCACAGGT
  CCCCCCACTGCTGGCCCTTCTACGGCCACTACTGTGCCTTTGAGTCCGGTGGACGATGCC
  TGCAACGTGAACATCTTCGACGCCATCGCGGAGATTGGGAACCAGCTGTATTTGTTCAAG
  GATGGGAAGTACTGGCGATTCTCTGAGGGCAGGGGGAGCCGGCCGCAGGGCCCCTTCCTT
  ATCGCCGACAAGTGGCCCGCGCTGCCCCGCAAGCTGGACTCGGTCTTTGAGGAGCCGCTC
  TCCAAGAAGCTTTTCTTCTTCTCTGGGCGCCAGGTGTGGGTGTACACAGGCGCGTCGGTG
  CTGGGCCCGAGGCGTCTGGACAAGCTGGGCCTGGGAGCCGACGTGGCCCAGGTGACCGGG
  GCCCTCCGGAGTGGCAGGGGGAAGATGCTGCTGTTCAGCGGGCGGCGCCTCTGGAGGTTC
  GACGTGAAGGCGCAGATGGTGGATCCCCGGAGCGCCAGCGAGGTGGACCGGATGTTCCCC
  GGGGTGCCTTTGGACACGCACGACGTCTTCCAGTACCGAGAGAAAGCCTATTTCTGCCAG
  GACCGCTTCTACTGGCGCGTGAGTTCCCGGAGTGAGTTGAACCAGGTGGACCAAGTGGGC
  TACGTGACCTATGACATCCTGCAGTGCCCTGAGGACTAGGGCTCCCGTCCTGCTTTGGCA
  GTGCCATGTAAATCCCCACTGGGACCAACCCTGGGGAAGGAGCCAGTTTGCCGGATACAA
  ACTGGTATTCTGTTCTGGAGGAAAGGGAGGAGTGGAGGTGGGCTGGGCCCTCTCTTCTCA
  CCTTTGTTTTTTGTTGGAGTGTTTCTAATAAACTTGGATTCTCTAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004994 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGAGCCTCTGGCAGCC
  CCTGGTCCTGGTGCTCCTGGTGCTGGGCTGCTGCTTTGCTGCCCCCAGACAGCGCCAGTC
  CACCCTTGTGCTCTTCCCTGGAGACCTGAGAACCAATCTCACCGACAGGCAGCTGGCAGA
  GGAATACCTGTACCGCTATGGTTACACTCGGGTGGCAGAGATGCGTGGAGAGTCGAAATC
  TCTGGGGCCTGCGCTGCTGCTTCTCCAGAAGCAACTGTCCCTGCCCGAGACCGGTGAGCT
  GGATAGCGCCACGCTGAAGGCCATGCGAACCCCACGGTGCGGGGTCCCAGACCTGGGCAG
  ATTCCAAACCTTTGAGGGCGACCTCAAGTGGCACCACCACAACATCACCTATTGGATCCA
  AAACTACTCGGAAGACTTGCCGCGGGCGGTGATTGACGACGCCTTTGCCCGCGCCTTCGC
  ACTGTGGAGCGCGGTGACGCCGCTCACCTTCACTCGCGTGTACAGCCGGGACGCAGACAT
  CGTCATCCAGTTTGGTGTCGCGGAGCACGGAGACGGGTATCCCTTCGACGGGAAGGACGG
  GCTCCTGGCACACGCCTTTCCTCCTGGCCCCGGCATTCAGGGAGACGCCCATTTCGACGA
  TGACGAGTTGTGGTCCCTGGGCAAGGGCGTCGTGGTTCCAACTCGGTTTGGAAACGCAGA
  TGGCGCGGNCTGCCACTTCCCCTTCATCTTCGNAGGCCGCTCCTACTCTGCCTGCACCAC
  CGACGGTCGCTCCGACGGCTTGCCCTGGTGCAGTACCACGGCCAACTACGACACCGACGA
  CCGGNTTGGCTTNCTGCCCAGCGAGAGACTCTACACCCGGGACNGGCATGCTGATGGGAA
  ACCCTGNCAGTTNCATTCATCTTCAGGNCCATACTACTNCGCTGCCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004994 unedited
  TTTTTTTTTTTNNTNTNNTNNTTTTTTTNTTTTTTGTNNTTTTTTTTTTTTCGATTTCTT
  CAGCAACACTTCCACAAAAACAAGGTGTACAACACGGGCCCAGTCCACTCCCTCCTCCTT
  TCCTCCAGAACAGATACCAGTTTGTATCCGGCAAACTGGCTCCTTCCCCAGGTTGGTCCC
  AGCGGGGATCCACATGGCACTGCCAACCCACGACGGCCCCCCCTAGTCCTCAGGGCACTG
  CCGGCACGTCACAGGTCCACGTCCCCCACTTGGCCCACCTGGTACAACTCACTTCGGGAA
  CTCACGCTTCATTCTTAATCCGTTCTCGCCCCAAATAGGCTCTCTCCTTCCTCCTGCTAC
  CCGTCCCCGCGTGCCCACACGCTCCCCGGTTGACCTTCCTGTCCCCCCTCCTGGCCCTCC
  CGGTGATTTACCCTCCTCTGCCTCCACTCCCTCACCTCCATAGGTCCTCGTTCTCTCACC
  ATCTCCTCTCTCCCCCTGCCCCCTCCCCAGGGTCACCTCCCCCCTGCGCCCACGTCCGCC
  TCCCCATGCCTCTTTTTGTTCACACCCCTCGCTTTCGATTTATTGCCGCACCCTGAGTAC
  ACCCCTCACCCCAGCTGCCCATCTTCACCCATCTAATCCTTCCCTGTCACGCCGTCCCCC
  CCCCCCACCGCACCTCCCCGCCTGCCACCGCCCCTGCCGCCCCCACCCCTCCCCCCCAAC
  CCCAAAAGCGCCCCCTGCCATCTTGGCCTCCCTCCTTCTCTTTATCAAACAACACAAACT
  CTGCCCTTCCCTCGTAAAATTTACTATTGCCACCCCCACCCCCCCCGTATGACACGCATA
  CCATGTTCATATTCCGCCCATATTTCACCCTATGAACTTACATTGTCCACTTCACGGGCC
  CGCATTATGGGCTCACCCTCTTGCGCCCCCCCCCTGTCTTCACTCCTCCCCGCCCTCCCC
  CACCTCTTCCCTCCCTCCCCCGCCCCCCTCCACCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004994
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004994.2, NP_004985.2
• RefSeq Size: 2387 bp
• RefSeq ORF: 2124 bp
• Locus ID: 4318
• Cytogenetics: 20q13.12
• Domains: FN2, hemopexin, Peptidase_M10, ZnMc, PT
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer
• Gene Summary: 'Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]'