NDUFB9 (NM_005005) Human Untagged Clone

CAT#: SC116999

NDUFB9 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa (NDUFB9), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFB9
• Synonyms: B22; CI-B22; LYRM3; MC1DN24; UQOR22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005005, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTTCTTGGCGTCGGGACCCTACCTGACCCATCAGCAAAAGGTGTTGCGGCTTTATAAGCGGGCGC
  TACGCCACCTCGAGTCGTGGTGCGTCCAGAGAGACAAATACCGATACTTTGCTTGTTTGATGAGAGCCCG
  GTTTGAAGAACATAAGAATGAAAAGGATATGGCGAAGGCCACCCAGCTGCTGAAGGAGGCCGAGGAAGAA
  TTCTGGTACCGTCAGCATCCACAGCCATACATCTTCCCTGACTCTCCTGGGGGCACCTCCTATGAGAGAT
  ACGATTGCTACAAGGTCCCAGAATGGTGCTTAGATGACTGGCATCCTTCTGAGAAGGCAATGTATCCTGA
  TTACTTTGCCAAGAGAGAACAGTGGAAGAAACTGCGGAGGGAAAGCTGGGAACGAGAGGTTAAGCAGCTG
  CAGGAGGAAACGCCACCTGGTGGTCCTTTAACTGAAGCTTTGCCCCCTGCCCGAAAGGAAGGTGATTTGC
  CCCCACTGTGGTGGTATATTGTGACCAGACCCCGGGAGCGGCCCATGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005005 unedited
  NGAATGTTCGCATTTTGTNATACGACTCCTATAGGCGGCCGCGNATTCGGCACCAGNAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
  GAGAGAGAGGGAAGGTCAGCGCCGTAATGGCGTTCTTGGCGTCGGGACCCTACCTGACCC
  ATCAGCAAAAGGTGTTGCGGCTTTATAAGCGGGCGCTACGCCACCTCGAGTCGTGGTGCG
  TCCAGAGAGACAAATACCGATACTTTGCTTGTTTGATGAGAGCCCGGTTTGAAGAACATA
  AGAATGAAAAGGATATGGCGAAGGCCACCCATCTGCTGAAGGAGGCCGAGGAAGAATTCT
  GGTACCGTCAGCATCCACAGCCATACATCTTCCCTGACTCTCCTGGGGGCACCTCCTATG
  AGAGATACGATTGCTACAAGGTCCCAGAATGGTGCTTATATGACTGGCATCCTTCTGAGA
  AGGCAATGTATCCTGATTACTTTGCCAAGAGAGAACAGTGGAAGAAACTGCGGAGGGAAA
  GCTGTGAACGAGAGGTTATGCATCTGCAGGAGGAAACGCCACCTGGTGGTCCTTTAACTG
  AAGCTTTGCCCCCTGCCCGAAAGGAAGGTGATTCTGCCCCCACTGTGGTGGTATATTGTG
  ACCAGACCCCGCGAGCGGCCCCTGTTTTAAAGAGAGAGACCTCATCTTTTCTGCTTGCCA
  GTTGAATATGTTTCAGAACATGCACTTTGCCCTATTTAAAAATCAGTGCAATTGGTCCAC
  AAAAACAAAACAATACTCTCTCTTTTTATTGCGGCCGCGGTCTTATTTGTTTTTTTGTCC
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005005 unedited
  GTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTGACCATTTCACTG
  ATTTTTTATTAAGGCAAGTGCATGTTCTGTAACATATTTCACTTGCAAGCATGAAAGATG
  AAGTCTCTCTCTTTCTACATGGGCCGCTCCCGGGGTCTGGTCACAATATACCACCACAGT
  GGGGGCAAATCACCTTCCTTTCGGGCAGGGGGCAAAGCTTCAGTTAAAGGACCACCAGGT
  GGCGTTTCCTCCTGCAGCTGCTTAACCTCTCGTTCCCAGCTTTCCCTCCGCAGTTTCTTC
  CACTGTTCTCTCTTGGCAAAGTAATCAGGATACATTGCCTTCTCAGAAAGATGCCAGTCA
  TCTAAGCACCATTCTGGGACCTTGTAGCAATCGTATCTCTCATATGAAGTGCCCCCAGGA
  GAGTCAAGGAAGATGTATGGCTGTGGATGCTGACGGTACCAAAATTCTTCCTCGGCCTCC
  TTCAGCAGCTGGGGGGCCCTCGCCAAAACTTTTTATTCTTATGCCTTCAACCGGCCTCTC
  TAAAAAACAAAGATCGGCATTTGCCTTCTGGACCCCCACAACTGAGGGGCGTACGCCCCT
  ATAATACACAACACCTTTCCTGCGGGCACGCACGCCCCACTCCAAACACCACACGGCCCC
  CACCCCCCCGTCTCTCTTCCCGCCCCCCCCCCCCCCCCCGTTCACACGGACAAAACTACC
  CGCCGAATCCTCACCCCCACCCCCTCACACGCGACTAATTCCCCGTCCCACTCGCCGCGC
  GCCACGACCAGCCACAACAGTGACCTAATCTCTTCTCAGCCACCCCATCTCCCACCTACC
  CGGATCCACCCGATCCACCATATACTATCTCCACCCCCCACATACACCACACACCCACCA
  ACACACTACAGCAGATCTCCTCATCCTCTCTGCAACACACCCCACCATCTCTACTCGCCG
  TTTCCCTATCTCCTTTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005005
• Insert Size: 860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005005.1, NP_004996.1
• RefSeq Size: 740 bp
• RefSeq ORF: 540 bp
• Locus ID: 4715
• Cytogenetics: 8q24.13
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).