NDUFS1 (NM_005006) Human Untagged Clone

CAT#: SC117000

NDUFS1 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_005006" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NDUFS1
Synonyms CI-75k; CI-75Kd; MC1DN5; PRO1304
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_005006, the custom clone sequence may differ by one or more nucleotides


ATGTTAAGGATACCTGTAAGAAAGGCCTTAGTAGGCCTTTCTAAGTCTCCTAAAGGATGTGTTCGAACAA
CTGCCACAGCAGCAAGCAACTTGATTGAAGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCGGGAAC
GACCGTCCTCCAAGCTTGTGAGAAGGTTGGCATGCAGATCCCTCGATTCTGTTATCATGAAAGGTTGTCT
GTTGCTGGAAACTGCAGGATGTGCCTTGTTGAAATTGAGAAAGCCCCTAAGGTTGTAGCTGCTTGTGCCA
TGCCAGTAATGAAGGGTTGGAATATCCTAACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGAT
GGAGTTCTTATTAGCAAATCACCCATTGGACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAG
GACCAGTCCATGATGTTTGGAAATGATAGGAGCCGATTTTTAGAGGGGAAGCGTGCTGTGGAAGACAAGA
ACATTGGGCCATTGGTAAAGACCATCATGACAAGATGTATACAGTGTACTCGCTGCATCAGGTTTGCAAG
TGAGATTGCAGGAGTAGATGATTTGGGAACAACAGGCAGAGGAAATGATATGCAAGTTGGCACATACATT
GAAAAGATGTTCATGTCTGAACTGTCTGGGAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTCTA
AGCCCTATGCCTTTACTGCCCGGCCTTGGGAAACAAGAAAGACAGAATCCATTGATGTAATGGATGCGGT
TGGAAGTAATATTGTGGTTAGCACAAGAACTGGAGAAGTGATGAGGATTTTGCCACGTATGCATGAGGAC
ATCAATGAAGAGTGGATCTCTGATAAAACCAGATTTGCCTATGATGGGCTAAAACGTCAAAGACTTACCG
AGCCAATGGTCAGAAATGAAAAAGGGCTTTTAACCTATACTTCTTGGGAGGATGCGCTCTCTCGCGTAGC
TGGAATGTTGCAGAGTTTTCAAGGCAAAGATGTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCC
CTGGTAGCTCTCAAAGATTTGCTTAATAGAGTGGACTCTGACACCTTATGCACTGAAGAGGTCTTCCCCA
CTGCAGGAGCTGGCACAGATTTGCGTTCCAATTATCTTCTTAATACTACAATTGCTGGTGTGGAAGAGGC
AGATGTTGTTCTTCTGGTTGGTACAAACCCACGTTTTGAGGCACCACTGTTTAATGCTAGAATTCGAAAG
AGCTGGCTGCATAATGACTTAAAAGTGGCCCTTATAGGCAGTCCAGTGGACCTCACTTACACATATGACC
ACCTGGGAGACTCCCCCAAAATTCTTCAAGACATTGCTTCGGGAAGCCATCCATTTAGCCAGGTCCTAAA
GGAAGCTAAAAAACCAATGGTGGTTTTAGGCAGTTCTGCACTCCAAAGAAATGATGGAGCAGCAATTCTT
GCAGCTGTTTCTAGCATTGCACAAAAGATTCGGATGACTAGTGGTGTTACTGGTGATTGGAAAGTTATGA
ATATCCTTCATAGGATTGCAAGTCAAGTAGCTGCTTTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAAT
TCGGAAGAACCCTCCCAAGGTGCTGTTTCTCCTGGGAGCAGATGGAGGTTGTATCACACGACAGGATTTG
CCAAAGGATTGTTTCATTATTTATCAAGGACATCATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTC
TCCCAGGAGCTGCTTACACAGAGAAGTCTGCTACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAA
GGTAGCAGTGACACCTCCTGGCTTGGCAAGAGAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCT
GGAATGACTCTTCCATATGATACTCTGGATCAAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTG
TTCGATATGATGATATTGAAGGGGCTAATTACTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCA
GCAGCTTCTTGCTGACCCACTTGTTCCACCTCAGCTAACTATAAAAGACTTCTACATGACAGATTCAATT
AGCAGAGCCTCACAGACAATGGCCAAATGTGTCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAAC
CATCCATATGCTGA


>OriGene 5' read for NM_005006 unedited
AAGTCACAAATTGTATCCGACTCATATAGGCGGCCGCGTAATTCGCACGAGCGGCCTCCT
AGGGGTCGTCGTGGTCCAGACAGTTTAGCAGAACAGCCTCCGCGGCATCCGGGGCAGAAG
CAATATGTTAAGGATACCTGTAAGAAAGGCCTTAGTAGGCCTTTCTAAGTCTCCTAAAGG
ATGTGTTCGAACAACTGCCACAGCAGCAAGCAACTTGATTGAAGTATTTGTTGATGGTCA
GTCTGTCATGGTGGAACCGGGAACGACCGTCCTCCAAGCTTGTGAGAAGGTTGGCATGCA
GATCCCTCGATTCTGTTATCATGAAAGGTTGTCTGTTGCTGGAAACTGCAGGATGTGCCT
TGTTGAAATTGAGAACGCCCCTAAGGTTGTAGCTGCTTGTGCCATGCCAGTAATGAAGGG
TTGGAATATCCTAACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGATGGAGTT
CTTATTAGCAAATCACCCATTGGACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCT
GCAGGACCAGTCCATGATGTTTGGAAATGATAGGAGCCGATTTTTAGAGGGGAAGCGTGC
TGTGGAAGACAAGAACATTGGGCCATTGGTAAAGACCATCATGACAAGATGTATACAGTG
TACTCCCTGCATCAGGTTTGCAAGTGAGATTGCAGGAGTAGATGATTTGGGAACAACAGG
CAGACTGAAAGATATGCAAGTTGCCACATACATTGAAAAGATCTTCATGTCTGAACTGCC
TCGGCAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTTTAAGCCCTATGCCTTTA
CTGGCCTGGCCTTGGGAAACCAGCAAGACCACATCCATTGATTGTCATGGATGCGGTTGG
CAAGCAAACTGTGGTTAACCCC
>OriGene 3' read for NM_005006 unedited
TCCAGGCCCGGTAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGGAT
CTCTTTATGTTCGTCACAAAGGTTATCAATGCTTTTAAGAGCATCTGCATAGTTTTGTTA
TTTAACCTTTACACACAATACATGTTTTTCAAACTGCAAAGTTGATAACTAATATCATTT
TCAAATAGGCATAGTATAACCTTAAATATTACATGATTCAAATTATTATTATTTTTTTTT
ACAAAGAAATAAACCTGTAAAGGATCACTGCACTACAGTTGTCCATTAATTATCTGCGGC
AAAACTGGGATCCTAGTAGAAGCTTCAGCATATGGATGGTTCCTCTACTGCCTGGGCACC
CTCTGTGACAGCTTTGACACATTTGGCCATTGTCTGTGAGGCTCTGCTAATTGAATCTGT
CATGTAGAAGTCTTTTATAGTTAGCTGAGGTGGAACAAGTGGGTCAGCAAGAAGCTGCTG
GTTCACTAGCTTTGAGAGCTCATTTGCTTGCTGGAAGTAATTAGCCCCTTCATATCATCA
TATCGAACAAGATTAGGAGAGACTTCTTCCAATCTGTTCCTTACTTGATCCAGAGTATCA
TATGGAAGAGTCATTCCAGNCATCTCAGAGAGTGCTCTTATNNATTTCCAGTCTTCTCTT
GCCAGCCCAGNAGNGTCACTGCTACCTTAGTCTGCTGAGCTCTACCCTCAGTGTGACATA
TGTANCAGACTTCTCTGTGGTAGCAGCTCTGGGAGAATACTCAGCTATGGGAGCCCACAT
ACCATGATGTCCTGATAATATGAAACANTCTTNGCACATCTGTCTGTGAACAACCTCTCT
GCTCAGGAGAAAAGACCTGTGGGAGGTCTTTCAAATGCTTCCCCCAGCTTATACCAGGGT
CAGCANNTCTGACTGCATCCTGAAGAATTCTACTTCAACCCA
Restriction Sites NotI-NotI     
ACCN NM_005006
Insert Size 2680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005006.5, NP_004997.4
RefSeq Size 3417 bp
RefSeq ORF 2184 bp
Locus ID 4719
Cytogenetics 2q33.3
Domains fer2, molybdopterin
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary 'The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
Transcript Variant: This variant (1) represents the longest and predominant transcript. Variant 1 encodes isoform 1.

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