NRF1 (NM_005011) Human Untagged Clone

CAT#: SC117004

NRF1 (untagged)-Human nuclear respiratory factor 1 (NRF1), transcript variant 1


  "NM_005011" in other vectors (4)

Reconstitution Protocol

USD 850.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NRF1
Synonyms ALPHA-PAL
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_005011, the custom clone sequence may differ by one or more nucleotides


ATGGAGGAACACGGAGTGACCCAAACCGAACATATGGCTACCATAGAAGCACATGCAGTGGCCCAGCAAG
TGCAGCAGGTCCATGTGGCTACTTACACCGAGCATAGTATGCTGAGTGCTGATGAAGACTCGCCTTCTTC
TCCCGAGGACACCTCTTACGATGACTCAGATATACTCAACTCCACAGCAGCTGATGAGGTGACAGCTCAT
CTGGCAGCTGCAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGAAAGAAACGGAAACGGC
CTCATGTATTTGAGTCTAATCCATCTATCCGGAAGAGGCAACAAACACGTTTGCTTCGGAAACTTCGAGC
CACGTTAGATGAATATACTACTCGTGTGGGACAGCAAGCTATTGTCCTCTGTATCTCACCCTCCAAACCT
AACCCTGTCTTTAAAGTGTTTGGTGCAGCACCTTTGGAGAATGTGGTGCGTAAGTACAAGAGCATGATCC
TGGAAGACCTGGAGTCTGCTCTGGCAGAACACGCCCCTGCGCCACAGGAGGTTAACTCAGAACTGCCGCC
TCTCACCATCGACGGAATTCCAGTCTCTGTGGACAAAATGACCCAGGCCCAGCTTCGGGCATTTATCCCA
GAGATGCTCAAGTACTCTACAGGTCGGGGAAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCCATCTGGT
GGCCTGAAGATATCCCCTGGGCAAATGTCCGGAGTGATGTCCGCACAGAAGAGCAAAAGCAGAGGGTTTC
ATGGACCCAGGCACTACGGACCATAGTTAAAAACTGTTATAAACAGCATGGGCGGGAAGACCTTTTGTAT
GCCTTTGAAGATCAGCAAACGCAAACACAGGCCACAGCCACACATAGTATAGCTCATCTTGTACCATCAC
AGACTGTAGTCCAGACTTTTAGTAACCCTGATGGCACTGTCTCACTTATCCAGGTTGGTACGGGGGCAAC
AGTAGCCACATTGGCTGATGCTTCAGAATTGCCAACCACGGTCACCGTTGCCCAAGTGAATTATTCTGCC
GTGGCTGATGGAGAGGTGGAACAAAATTGGGCCACGTTACAGGGAGGTGAGATGACCATCCAGACGACGC
AAGCATCAGAGGCCACCCAGGCGGTGGCATCGTTGGCAGAGGCCGCAGTGGCAGCTTCTCAGGAGATGCA
GCAGGGAGCTACAGTCACTATGGCGCTTAACAGCGAAGCTGCCGCCCATGCTGTCGCCACCCTGGCTGAG
GCCACCTTACAAGGTGGGGGACAGATCGTCTTGTCTGGGGAAACCGCAGCAGCCGTCGGAGCACTTACTG
GAGTCCAAGATGCTAATGGCCTGGTCCAGATCCCTGTGAGCATGTACCAGACTGTGGTGACCAGCCTCGC
CCAGGGCAACGGACCAGTGCAGGTGGCCATGGCCCCTGTGACCACCAGGATATCAGACAGCGCAGTCACC
ATGGACGGCCAAGCTGTGGAGGTGGTGACATTGGAACAGTGA


>OriGene 5' read for NM_005011 unedited
CGCAATAGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGCATTGCCGCTGGT
GGCAGGAGGCTGCGAGGAGCCGGCGCGGTCGCAGTCTCCACGGCGCAGGCCCACGGTAGC
GCAGCCGCTCTGAGAACTTCATGGAGGAACACGGAGTGACCCAAACCGAACATATGGCTA
CCATAGAAGCACATGCAGTGGCCCAGCAAGTGCAGCAGGTCCATGTGGCTACTTACACCG
AGCATAGTATGCTGAGTGCTGATGAAGACTCGCCTTCTTCTCCCGAGGACACCTCTTACG
ATGACTCAGATATACTCAACTCCACAGCAGCTGATGAGGTGACAGCTCATCTGGCAGCTG
CAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGAAAGAAACGGAAACGGC
CTCATGTATTTGAGTCTAATCCATCTATCCGGAAGAGGCAACAAACACGTTTGCTTCGGA
AACTTCGAGCCACGTTAGATGAATATACTACTCGTGTGGGACAGCAAGCTATTGTCCTCT
GTATCTCACCCTCCAAACCTAACCCTGTCTTTAAAGTGTTTGGTGCAGCACCTTTGGAGA
ATGTGGTGCGTAAGTACAAGAGCATGATCCTGGAAGACCTGGAGTCTGCTCTGGCAGAAC
ACGCCCCTGCGCCACAGGAGGTTAACTCAGAACTGCCGCCTCTCACCATCGATGGAATTC
CAGTCTCTGTGGACAAAATGACCCAGGCCCAGCTTCGGGCATTTATCCCAGAGATGCTCA
AGTACCTCTACAGGTCCGGGGAAAACCAGGCTGGGGGAAAGAAAGCTGCAGGCCATNTTG
TGGCCTGAAGAATCCCCTGGCCAAAGTCCGGATGATGTCCGCCNCAGAGAGCAAAGCAGA
GGGTTGGTACCGGGGCAACAGTAACCACATTGGCTGATGCCTTAAAATGCCACCN
>OriGene 3' read for NM_005011 unedited
CGGGGGAGCCNNCCCCTTNNNNNNNGANGATCTTGNCCGCGGCCGCATCTANGANCGAGN
NNTTNTTTTTTTTTTTTTTTTTTTTTTGACCTTAAAACCAGACAACATTTTAATAGTCAT
GAGATCAAGGATTCCTGGGAAGGAAAGGAGATGGGAGGCTGAGGCCCGGGAGCTTTCAAG
ACATTCATCTGCCTGTGGCAAGGTCAGCACCTGGGCCAGCCCGAGGAGCATCTCAGCTGG
CAGACCACGCAGTCCCTGGACCTTGCAAGCACATCTCTGACCCACCCCATGCTGGAGCCT
CTTTCCTCAGGTGAGTCCAGCAGGGAGAGTTCTGTGTGGCCACTGGGAGGCAGCTTTGGT
GAAGGGCATGGTCACCTCCGCCTGAGTTTGTCAGTAGCTCAACGCATGACCCTGGTATGA
ACAAAAACTGGGTGACTGTGTCTGGCCACGTACCCTACCACCCACTAAACTGGGTGAATT
ATTGTTTCCACAGCGCACCTTGATTTCATACAGATAATTCCATGCGGGTTTCATACATAT
ATATATATATATGGATATATATACTTTATATATATGTAAATGTGTATGTCCATATGTACA
CATATACATATATTCACACTCACACAACACACACATAAGTTTACACACACATTTCCGCAG
AAGAAAGCCTGCCACAANTTGGCTTTTTTTGGACAGTGAANATGACAATGCAGTTTCCTC
ACCCATCCCAGAAAATCAGAAGTGTGCTTTAAAAATGCATTTCAAAATCGCTTTTTTCTT
TTAAAAAAAAAGGTTACAAACTTTNCTTCAAAGTCAAAGACTTATTCCACTTGATGGGCC
TTTGCAACGTGTAAAATTTTTGAGGTGACTGAAACCATGCCTTAAATGGCTGATGTCACT
GTCCATGTACCACCTCCCAGTTGGCCGCCATGGGACTGGCCTTTTGATATCTGGGGCCAG
GGGCCATGCCACTGCCTGGTCCTGCCCTGGCAAGCTGGCCACAAGT
Restriction Sites NotI-NotI     
ACCN NM_005011
Insert Size 2440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005011.2, NP_005002.2
RefSeq Size 2514 bp
RefSeq ORF 1569 bp
Locus ID 4899
Cytogenetics 7q32.2
Protein Families Transcription Factors
Protein Pathways Huntington's disease
Gene Summary 'This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]'
Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The encoded protein (isoform 1) is shorter, compared to isoform 2. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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