NRF1 (NM_005011) Human Untagged Clone

CAT#: SC117004

NRF1 (untagged)-Human nuclear respiratory factor 1 (NRF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRF1
• Synonyms: ALPHA-PAL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005011, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGAACACGGAGTGACCCAAACCGAACATATGGCTACCATAGAAGCACATGCAGTGGCCCAGCAAG
  TGCAGCAGGTCCATGTGGCTACTTACACCGAGCATAGTATGCTGAGTGCTGATGAAGACTCGCCTTCTTC
  TCCCGAGGACACCTCTTACGATGACTCAGATATACTCAACTCCACAGCAGCTGATGAGGTGACAGCTCAT
  CTGGCAGCTGCAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGAAAGAAACGGAAACGGC
  CTCATGTATTTGAGTCTAATCCATCTATCCGGAAGAGGCAACAAACACGTTTGCTTCGGAAACTTCGAGC
  CACGTTAGATGAATATACTACTCGTGTGGGACAGCAAGCTATTGTCCTCTGTATCTCACCCTCCAAACCT
  AACCCTGTCTTTAAAGTGTTTGGTGCAGCACCTTTGGAGAATGTGGTGCGTAAGTACAAGAGCATGATCC
  TGGAAGACCTGGAGTCTGCTCTGGCAGAACACGCCCCTGCGCCACAGGAGGTTAACTCAGAACTGCCGCC
  TCTCACCATCGACGGAATTCCAGTCTCTGTGGACAAAATGACCCAGGCCCAGCTTCGGGCATTTATCCCA
  GAGATGCTCAAGTACTCTACAGGTCGGGGAAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCCATCTGGT
  GGCCTGAAGATATCCCCTGGGCAAATGTCCGGAGTGATGTCCGCACAGAAGAGCAAAAGCAGAGGGTTTC
  ATGGACCCAGGCACTACGGACCATAGTTAAAAACTGTTATAAACAGCATGGGCGGGAAGACCTTTTGTAT
  GCCTTTGAAGATCAGCAAACGCAAACACAGGCCACAGCCACACATAGTATAGCTCATCTTGTACCATCAC
  AGACTGTAGTCCAGACTTTTAGTAACCCTGATGGCACTGTCTCACTTATCCAGGTTGGTACGGGGGCAAC
  AGTAGCCACATTGGCTGATGCTTCAGAATTGCCAACCACGGTCACCGTTGCCCAAGTGAATTATTCTGCC
  GTGGCTGATGGAGAGGTGGAACAAAATTGGGCCACGTTACAGGGAGGTGAGATGACCATCCAGACGACGC
  AAGCATCAGAGGCCACCCAGGCGGTGGCATCGTTGGCAGAGGCCGCAGTGGCAGCTTCTCAGGAGATGCA
  GCAGGGAGCTACAGTCACTATGGCGCTTAACAGCGAAGCTGCCGCCCATGCTGTCGCCACCCTGGCTGAG
  GCCACCTTACAAGGTGGGGGACAGATCGTCTTGTCTGGGGAAACCGCAGCAGCCGTCGGAGCACTTACTG
  GAGTCCAAGATGCTAATGGCCTGGTCCAGATCCCTGTGAGCATGTACCAGACTGTGGTGACCAGCCTCGC
  CCAGGGCAACGGACCAGTGCAGGTGGCCATGGCCCCTGTGACCACCAGGATATCAGACAGCGCAGTCACC
  ATGGACGGCCAAGCTGTGGAGGTGGTGACATTGGAACAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005011 unedited
  CGCAATAGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGCATTGCCGCTGGT
  GGCAGGAGGCTGCGAGGAGCCGGCGCGGTCGCAGTCTCCACGGCGCAGGCCCACGGTAGC
  GCAGCCGCTCTGAGAACTTCATGGAGGAACACGGAGTGACCCAAACCGAACATATGGCTA
  CCATAGAAGCACATGCAGTGGCCCAGCAAGTGCAGCAGGTCCATGTGGCTACTTACACCG
  AGCATAGTATGCTGAGTGCTGATGAAGACTCGCCTTCTTCTCCCGAGGACACCTCTTACG
  ATGACTCAGATATACTCAACTCCACAGCAGCTGATGAGGTGACAGCTCATCTGGCAGCTG
  CAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGAAAGAAACGGAAACGGC
  CTCATGTATTTGAGTCTAATCCATCTATCCGGAAGAGGCAACAAACACGTTTGCTTCGGA
  AACTTCGAGCCACGTTAGATGAATATACTACTCGTGTGGGACAGCAAGCTATTGTCCTCT
  GTATCTCACCCTCCAAACCTAACCCTGTCTTTAAAGTGTTTGGTGCAGCACCTTTGGAGA
  ATGTGGTGCGTAAGTACAAGAGCATGATCCTGGAAGACCTGGAGTCTGCTCTGGCAGAAC
  ACGCCCCTGCGCCACAGGAGGTTAACTCAGAACTGCCGCCTCTCACCATCGATGGAATTC
  CAGTCTCTGTGGACAAAATGACCCAGGCCCAGCTTCGGGCATTTATCCCAGAGATGCTCA
  AGTACCTCTACAGGTCCGGGGAAAACCAGGCTGGGGGAAAGAAAGCTGCAGGCCATNTTG
  TGGCCTGAAGAATCCCCTGGCCAAAGTCCGGATGATGTCCGCCNCAGAGAGCAAAGCAGA
  GGGTTGGTACCGGGGCAACAGTAACCACATTGGCTGATGCCTTAAAATGCCACCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005011 unedited
  CGGGGGAGCCNNCCCCTTNNNNNNNGANGATCTTGNCCGCGGCCGCATCTANGANCGAGN
  NNTTNTTTTTTTTTTTTTTTTTTTTTTGACCTTAAAACCAGACAACATTTTAATAGTCAT
  GAGATCAAGGATTCCTGGGAAGGAAAGGAGATGGGAGGCTGAGGCCCGGGAGCTTTCAAG
  ACATTCATCTGCCTGTGGCAAGGTCAGCACCTGGGCCAGCCCGAGGAGCATCTCAGCTGG
  CAGACCACGCAGTCCCTGGACCTTGCAAGCACATCTCTGACCCACCCCATGCTGGAGCCT
  CTTTCCTCAGGTGAGTCCAGCAGGGAGAGTTCTGTGTGGCCACTGGGAGGCAGCTTTGGT
  GAAGGGCATGGTCACCTCCGCCTGAGTTTGTCAGTAGCTCAACGCATGACCCTGGTATGA
  ACAAAAACTGGGTGACTGTGTCTGGCCACGTACCCTACCACCCACTAAACTGGGTGAATT
  ATTGTTTCCACAGCGCACCTTGATTTCATACAGATAATTCCATGCGGGTTTCATACATAT
  ATATATATATATGGATATATATACTTTATATATATGTAAATGTGTATGTCCATATGTACA
  CATATACATATATTCACACTCACACAACACACACATAAGTTTACACACACATTTCCGCAG
  AAGAAAGCCTGCCACAANTTGGCTTTTTTTGGACAGTGAANATGACAATGCAGTTTCCTC
  ACCCATCCCAGAAAATCAGAAGTGTGCTTTAAAAATGCATTTCAAAATCGCTTTTTTCTT
  TTAAAAAAAAAGGTTACAAACTTTNCTTCAAAGTCAAAGACTTATTCCACTTGATGGGCC
  TTTGCAACGTGTAAAATTTTTGAGGTGACTGAAACCATGCCTTAAATGGCTGATGTCACT
  GTCCATGTACCACCTCCCAGTTGGCCGCCATGGGACTGGCCTTTTGATATCTGGGGCCAG
  GGGCCATGCCACTGCCTGGTCCTGCCCTGGCAAGCTGGCCACAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005011
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005011.2, NP_005002.2
• RefSeq Size: 2514 bp
• RefSeq ORF: 1569 bp
• Locus ID: 4899
• Cytogenetics: 7q32.2
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease
• Gene Summary: 'This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The encoded protein (isoform 1) is shorter, compared to isoform 2. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.