PCYT1A (NM_005017) Human Untagged Clone

CAT#: SC117010

PCYT1A (untagged)-Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A)


  "NM_005017" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PCYT1A
Synonyms CCTA; CT; CTA; CTPCT; PCYT1; SMDCRD
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117010 sequence for NM_005017 edited (data generated by NextGen Sequencing)
ATGGATGCACAGTGTTCAGCCAAGGTCAATGCAAGGAAGAGGAGAAAAGAGGCGCCCGGA
CCCAACGGGGCAACAGAAGAAGATGGGGTTCCTTCCAAAGTGCAGCGCTGTGCAGTGGGC
TTACGGCAACCAGCTCCTTTTTCTGATGAAATTGAAGTTGACTTTAGTAAGCCCTATGTC
AGGGTAACTATGGAAGAAGCCAGCAGAGGAACTCCTTGTGAGCGACCTGTGAGAGTTTAT
GCCGATGGAATATTTGACTTATTTCACTCTGGTCACGCCCGAGCTCTGATGCAAGCGAAG
AACCTTTTCCCTAATACGTACCTCATTGTGGGAGTTTGCAGTGATGAGCTCACACACAAC
TTCAAAGGCTTCACGGTGATGAACGAGAATGAGCGCTATGACGCAGTCCAGCACTGCCGC
TACGTGGATGAGGTGGTGAGGAATGCGCCCTGGACGCTGACACCCGAGTTCCTGGCCGAA
CACCGGATTGATTTTGTAGCCCATGATGATATTCCTTATTCATCTGCTGGCAGTGATGAT
GTTTATAAGCACATCAAGGAGGCAGGCATGTTTGCTCCAACACAGAGGACAGAAGGTATC
TCCACATCAGACATCATCACCCGAATTGTGCGGGATTATGATGTGTATGCGAGGCGGAAC
CTGCAGAGGGGCTACACAGCAAAGGAGCTCAATGTCAGCTTTATCAACGAGAAGAAATAC
CACTTGCAGGAGAGGGTTGACAAAGTAAAGAAGAAAGTGAAAGATGTGGAGGAAAAGTCA
AAAGAATTTGTTCAGAAGGTGGAGGAAAAAAGCATTGACCTCATTCAGAAGTGGGAGGAG
AAGTCCCGAGAATTCATTGGAAGTTTTCTGGAAATGTTTGGTCCGGAAGGAGCACTGAAA
CATATGCTGAAAGAGGGGAAGGGCCGGATGCTGCAGGCCATCAGCCCGAAGCAGAGCCCC
AGCAGCAGCCCTACTCGCGAGCGCTCCCCCTCCCCCTCTTTCCGATGGCCCTTCTCCGGC
AAGACTTCCCCACCTTGCTCCCCAGCAAATCTCTCCAGGCACAAGGCTGCAGCCTATGAT
ATCAGTGAGGATGAAGAAGACTAA

Clone variation with respect to NM_005017.2
>OriGene 5' read for NM_005017 unedited
NGGTTAGGTTTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGGCTCAAC
TTTCCCTAGTCAGTGACGCGGCGCCGGCCAGAAATCCGACCGGACCGGGCTCGGGGGAGC
GTGAGTTGCAGTTAAAAGAAGATGGATGCACAGTGTTCAGCCAAGGTCAATGCAAGGAAG
AGGAGAAAAGAGGCGCCCGGACCCAACGGGGCAACAGAAGAAGATGGGGTTCCTTCCAAA
GTGCAGCGCTGTGCAGTGGGCTTACGGCAACCAGCTCCTTTTTCTGATGAAATTGAAGTT
GACTTTAGTAAGCCCTATGTCAGGGTAACTATGGAAGAAGCCAGCAGAGGAACTCCTTGT
GAGCGACCTGTGAGAGTTTATGCCGATGGAATATTTGACTTATTTCACTCTGGTCACGCC
CGAGCTCTGATGCAAGCGAAGAACCTTTTCCCTAATACGTACCTCATTGTGGGAGACTTT
TTTCCAGTTGGGGAAGATGGTTGCAGTCCTAAGCAGTGGGAGAAGATGGGAGAGGTCGGT
GGCTGAGGAGGTGCTAAGCAAAGAGGACTCCAACACACAATTTATGATGTGGTGAACCAG
TTTGCAGTGATGAGCTCACACACAACTTCAAAGGCTTCACGGTGATGAACGAGAATGAGC
GCTATGACGCAGTCCAGCACTGCCGCTACGTGGATGAGGTGGTGAGGAATGCGCCCTGGA
CGCTGACACCCGAGTTCCTGGCCGAACACCGGATTGATTTTGTAGCCCATGATGATATTC
CTTATTCATCTGCTGGCAGTGATGATGTTATAAGCACATCANGGAGCAGGCCTGTTTGCT
TCACACAGAGACAGAAGGATCTCCACATAGACTCATACCCGAATGTGCGGATATGATGTG
ATGCGAGCCGGACTGCAGAGGGGTACCAGAAG
Restriction Sites NotI-NotI     
ACCN NM_005017
Insert Size 3570 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005017.2, NP_005008.2
RefSeq Size 1677 bp
RefSeq ORF 1104 bp
Locus ID 5130
Cytogenetics 3q29
Domains CTP_transf_2
Protein Pathways Glycerophospholipid metabolism, Metabolic pathways
Gene Summary 'This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]'
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

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