PCYT1A (NM_005017) Human Untagged Clone

CAT#: SC117010

PCYT1A (untagged)-Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCYT1A
• Synonyms: CCTA; CT; CTA; CTPCT; PCYT1; SMDCRD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117010 sequence for NM_005017 edited (data generated by NextGen Sequencing)
  ATGGATGCACAGTGTTCAGCCAAGGTCAATGCAAGGAAGAGGAGAAAAGAGGCGCCCGGA
  CCCAACGGGGCAACAGAAGAAGATGGGGTTCCTTCCAAAGTGCAGCGCTGTGCAGTGGGC
  TTACGGCAACCAGCTCCTTTTTCTGATGAAATTGAAGTTGACTTTAGTAAGCCCTATGTC
  AGGGTAACTATGGAAGAAGCCAGCAGAGGAACTCCTTGTGAGCGACCTGTGAGAGTTTAT
  GCCGATGGAATATTTGACTTATTTCACTCTGGTCACGCCCGAGCTCTGATGCAAGCGAAG
  AACCTTTTCCCTAATACGTACCTCATTGTGGGAGTTTGCAGTGATGAGCTCACACACAAC
  TTCAAAGGCTTCACGGTGATGAACGAGAATGAGCGCTATGACGCAGTCCAGCACTGCCGC
  TACGTGGATGAGGTGGTGAGGAATGCGCCCTGGACGCTGACACCCGAGTTCCTGGCCGAA
  CACCGGATTGATTTTGTAGCCCATGATGATATTCCTTATTCATCTGCTGGCAGTGATGAT
  GTTTATAAGCACATCAAGGAGGCAGGCATGTTTGCTCCAACACAGAGGACAGAAGGTATC
  TCCACATCAGACATCATCACCCGAATTGTGCGGGATTATGATGTGTATGCGAGGCGGAAC
  CTGCAGAGGGGCTACACAGCAAAGGAGCTCAATGTCAGCTTTATCAACGAGAAGAAATAC
  CACTTGCAGGAGAGGGTTGACAAAGTAAAGAAGAAAGTGAAAGATGTGGAGGAAAAGTCA
  AAAGAATTTGTTCAGAAGGTGGAGGAAAAAAGCATTGACCTCATTCAGAAGTGGGAGGAG
  AAGTCCCGAGAATTCATTGGAAGTTTTCTGGAAATGTTTGGTCCGGAAGGAGCACTGAAA
  CATATGCTGAAAGAGGGGAAGGGCCGGATGCTGCAGGCCATCAGCCCGAAGCAGAGCCCC
  AGCAGCAGCCCTACTCGCGAGCGCTCCCCCTCCCCCTCTTTCCGATGGCCCTTCTCCGGC
  AAGACTTCCCCACCTTGCTCCCCAGCAAATCTCTCCAGGCACAAGGCTGCAGCCTATGAT
  ATCAGTGAGGATGAAGAAGACTAA
  
  Clone variation with respect to NM_005017.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005017 unedited
  NGGTTAGGTTTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGGCTCAAC
  TTTCCCTAGTCAGTGACGCGGCGCCGGCCAGAAATCCGACCGGACCGGGCTCGGGGGAGC
  GTGAGTTGCAGTTAAAAGAAGATGGATGCACAGTGTTCAGCCAAGGTCAATGCAAGGAAG
  AGGAGAAAAGAGGCGCCCGGACCCAACGGGGCAACAGAAGAAGATGGGGTTCCTTCCAAA
  GTGCAGCGCTGTGCAGTGGGCTTACGGCAACCAGCTCCTTTTTCTGATGAAATTGAAGTT
  GACTTTAGTAAGCCCTATGTCAGGGTAACTATGGAAGAAGCCAGCAGAGGAACTCCTTGT
  GAGCGACCTGTGAGAGTTTATGCCGATGGAATATTTGACTTATTTCACTCTGGTCACGCC
  CGAGCTCTGATGCAAGCGAAGAACCTTTTCCCTAATACGTACCTCATTGTGGGAGACTTT
  TTTCCAGTTGGGGAAGATGGTTGCAGTCCTAAGCAGTGGGAGAAGATGGGAGAGGTCGGT
  GGCTGAGGAGGTGCTAAGCAAAGAGGACTCCAACACACAATTTATGATGTGGTGAACCAG
  TTTGCAGTGATGAGCTCACACACAACTTCAAAGGCTTCACGGTGATGAACGAGAATGAGC
  GCTATGACGCAGTCCAGCACTGCCGCTACGTGGATGAGGTGGTGAGGAATGCGCCCTGGA
  CGCTGACACCCGAGTTCCTGGCCGAACACCGGATTGATTTTGTAGCCCATGATGATATTC
  CTTATTCATCTGCTGGCAGTGATGATGTTATAAGCACATCANGGAGCAGGCCTGTTTGCT
  TCACACAGAGACAGAAGGATCTCCACATAGACTCATACCCGAATGTGCGGATATGATGTG
  ATGCGAGCCGGACTGCAGAGGGGTACCAGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005017
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005017.2, NP_005008.2
• RefSeq Size: 1677 bp
• RefSeq ORF: 1104 bp
• Locus ID: 5130
• Cytogenetics: 3q29
• Domains: CTP_transf_2
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: 'This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.