PD1 (PDCD1) (NM_005018) Human Untagged Clone

CAT#: SC117011

PD-1 / PDCD1 (untagged)-Human programmed cell death 1 (PD-1 / PDCD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDCD1
• Synonyms: CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005018 edited
  GAATTCGGCACGAGGTGGAGAAGGCGGCACTCTGGTGGGGCTGCTCCAGGCATGCAGATC
  CCACAGGCGCCCTGGCCAGTCGTCTGGGCGGTGCTACAACTGGGCTGGCGGCCAGGATGG
  TTCTTAGACTCCCCAGACAGGCCCTGGAACCCCCCCACCTTCTCCCCAGCCCTGCTCGTG
  GTGACCGAAGGGGACAACGCCACCTTCACCTGCAGCTTCTCCAACACATCGGAGAGCTTC
  GTGCTAAACTGGTACCGCATGAGCCCCAGCAACCAGACGGACAAGCTGGCCGCCTTCCCC
  GAGGACCGCAGCCAGCCCGGCCAGGACTGCCGCTTCCGTGTCACACAACTGCCCAACGGG
  CGTGACTTCCACATGAGCGTGGTCAGGGCCCGGCGCAATGACAGCGGCACCTACCTCTGT
  GGGGCCATCTCCCTGGCCCCCAAGGCGCAGATCAAAGAGAGCCTGCGGGCAGAGCTCAGG
  GTGACAGAGAGAAGGGCAGAAGTGCCCACAGCCCACCCCAGCCCCTCACCCAGGCCAGCC
  GGCCAGTTCCAAACCCTGGTGGTTGGTGTCGTGGGCGGCCTGCTGGGCAGCCTGGTGCTG
  CTAGTCTGGGTCCTGGCCGTCATCTGCTCCCGGGCCGCACGAGGGACAATAGGAGCCAGG
  CGCACCGGCCAGCCCCTGAAGGAGGACCCCTCAGCCGTGCCTGTGTTCTCTGTGGACTAT
  GGGGAGCTGGATTTCCAGTGGCGAGAGAAGACCCCGGAGCCCCCCGTGCCCTGTGTCCCT
  GAGCAGACGGAGTATGCCACCATTGTCTTTCCTAGCGGAATGGGCACCTCATCCCCCGCC
  CGCAGGGGCTCAGCCGACGGCCCTCGGAGTGCCCAGCCACTGAGGCCTGAGGATGGACAC
  TGCTCTTGGCCCCTCTGACCGGCTTCCTTGGCCACCAGTGTTCTGCAGACCCTCCACCAT
  GAGCCCGGGTCAGCGCATTTCCTCAGGAGAAGCAGGCAGGGTGCAGGCCATTGCAGGCCG
  TCCAGGGGCTGAGCTGCCTGGGGGCGACCGGGGCTCCAGCCTGCACCTGCACCAGGCACA
  GCCCCACCACAGGACTCATGTCTCAATGCCCACAGTGAGCCCAGGCAGCAGGTGTCACCG
  TCCCCTACAGGGAGGGCCAGATGCAGTCACTGCTTCAGGTCCTGCCAGCACAGAGCTGCC
  TGCGTCCAGCTCCCTGAATCTCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCCTGCGGCC
  CGGGGCTGAAGGCGCCGTGGCCCTGCCTGACGCCCCGGAGCCTCCTGCCTGAACTTGGGG
  GCTGGTTGGAGATGGCCTTGGAGCAGCCAAGGTGCCCCTGGCAGTGGCATCCCGAAACGC
  CCTGGACGCAGGGCCCAAGACTGGGCACAGGAGTGGGAGGTACATGGGGCTGGGGACTCC
  CCAGGAGTTATCTGCTCCCTGCAGGCCTAGAGAAGTTTCAGGGAAGGTCAGAAGAGCTCC
  TGGCTGTGGTGGGCAGGGCAGGAAACCCCTCCACCTTTACACATGCCCAGGCAGCACCTC
  AGGCCCTTTGTGGGGCAGGGAAGCTGAGGCAGTAAGCGGGCAGGCAGAGCTGGAGGCCTT
  TCAGGCCCAGCCAGCACTCTGGCCTCCTGCCGCCGCATTCCACCCCAGCCCCTCACACCA
  CTCGGGAGAGGGACATCCTACGGTCCCAAGGTCAGGAGGGCAGGGCTGGGGTTGACTCAG
  GCCCCTCCCAGCTGTGGCCACCTGGGTGTTGGGAGGGCAGAAGTGCAGGCACCTAGGGCC
  CCCCATGTGCCCACCCTGGGAGCTCTCCTTGGAACCCATTCCTGAAATTATTTAAAGGGG
  TTGGCCGGGCTCCCACCAGGGCCTGGGTGGGAAGGTACAGGCGTTCCCCCGGGGCCTAGT
  ACCCCCGCCGTGGCCTATCCACTCCTCACATCCACACACTGCACCCCCACTCCTGGGGCA
  GGGCCACCAGCATCCAGGCGGCCAGCAGGCACCTGAGTGGCTGGGACAAGGGATCCCCCT
  TCCCTGTGGTTCTATTATATTATAATTATAATTAAATATGAGAGCATGCCAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005018 unedited
  GCATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGGAGAAGGC
  GGCACTCTGGTGGGGCTGCTCCAGGCATGCAGATCCCACAGGCGCCCTGGCCAGTCGTCT
  GGGCGGTGCTACAACTGGGCTGGCGGCCAGGATGGTTCTTAGACTCCCCAGACAGGCCCT
  GGAACCCCCCCACCTTCTCCCCAGCCCTGCTCGTGGTGACCGAAGGGGACAACGCCACCT
  TCACCTGCAGCTTCTCCAACACATCGGAGAGCTTCGTGCTAAACTGGTACCGCATGAGCC
  CCAGCAACCAGACGGACAAGCTGGCCGCCTTCCCCGAGGACCGCAGCCAGCCCGGCCAGG
  ACTGCCGCTTCCGTGTCACACAACTGCCCAACGGGCGTGACTTCCACATGAGCGTGGTCA
  GGGCCCGGCGCAATGACAGCGGCACCTACCTCTGTGGGGCCATCTCCCTGGCCCCCAAGG
  CGCAGATCAAAGAGAGCCTGCGGGCAGAGCTCAGGGTGACAGAGAGAAGGGCAGAAGTGC
  CCACAGCCCACCCCAGCCCCTCACCCAGGCCAGCCGGCCAGTTCCAAACCCTGGTGGTTG
  GTGTCGTGGGCGGCCTGCTGGGCAGCCTGGTGCTGCTAGTCTGGGTCCTGGCCGTCATCT
  GCTCCCGGGCCGCACGAGGGACAATAGGAGCCAGGCGCACCGGCCAGCNCCCTGAAGAGG
  ACCCCTCAGCCGTGCCTGTGTTCTCTGTGGACTATGGGGAGCTGGATTTCCAGTGGCGAG
  AGAAGACCCCGGACCCCCCGTGCCCTGTGTCCCTGAGCAGACGGAGTATGCCACCATTGT
  CTTTCCTAGCGGAATGGGCACCTCATNCNCCGCCCGCAGGGGCTCAGCCGACGGCCCTCG
  GAGTGCCCAGCCCTGAGGCCTGAGATGGACCTGCTCTTGCCCTCTGACGGCTNCTGGCCC
  CAGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005018 unedited
  CGCGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCATGCTCTCATATTTAA
  TTATAATTATAATATAATAGAACCACAGGGAAGGGGGATCCCTTGTCCCAGCCACTCAGG
  TGCCTGCTGGCCGCCTGGATGCTGGTGGCCCTGCCCCAGGAGTGGGGGTGCAGTGTGTGG
  ATGTGAGGAGTGGATAGGCCACGGCGGGGGTACTAGGCCCCGGGGGAACGCCTGTACCTT
  CCCACCCAGGCCCTGGTGGGAGCCCGGCCAACCCCTTTAAATAATTTCAGGAATGGGTTC
  CAAGGAGAGCTCCCAGGGTGGGCACATGGGGGGCCCTAGGTGCCTGCACTTCTGCCCTCC
  CAACACCCAGGTGGCCACAGCTGGGAGGGGCCTGAGTCAACCCCAGCCCTGCCCTCCTGA
  CCTTGGGACCGTAGGATGTCCCTCTCCCGAGTGGTGTGAGGGGCTGGGGTGGAATGCGGC
  GGCAGGAGGCCAGAGTGCTGGCTGGGCCTGAAAGGCCTCCAGCTCTGCCTGCCCGCTTAC
  TGCCTCAGCTTTCCTGCCCCACAAAGGGCCTGAGGTGCTGCCTGGGCATGTGTAAAGGTG
  GAGGGGTTTCCTGCCCTGCCCACCACAGCCAGGAGCTCTTCTGACCTTTCCTGAAACTTC
  TCTAGGCCTGCAGGGAGCAGATAACTTCTGGGGAGTCCCCAGCCCCATTGTCCTTCCAAT
  TCTGTGCCCAGTCTTGGGCCCTGCGCCCAGGGCGTTTTGGGATGCCCCTGCCCGGGGCCC
  CTTTGCTGGTCCAAGCCATTTCCAACCAACCCCAAGTTCAGCAGGAAGTTCCGGGCGTAA
  GGAAGGGCCCGCGCCTTTAACCCCGGCCCCATGCACTACTACATCATATCATCAGTTCCG
  AGTATATGGAGCTGGCCCCAGCACTTTTTGTCTGCAGGACTCGAAACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005018
• Insert Size: 2240 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005018.1, NP_005009.1
• RefSeq Size: 2106 bp
• RefSeq ORF: 867 bp
• Locus ID: 5133
• Cytogenetics: 2q37.3
• Domains: ig, IGv, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), T cell receptor signaling pathway
• Gene Summary: 'Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020]'