Kv2.1 (KCNB1) (NM_004975) Human Untagged Clone

CAT#: SC117021

KCNB1 (untagged)-Human potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNB1
• Synonyms: DRK1; Kv2.1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004975, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGGCGGGCATGACGAAGCATGGCTCCCGCTCCACCAGCTCGCTGCCGCCCGAGCCCATGGAGATCG
  TGCGCAGCAAGGCGTGCTCTCGGCGGGTCCGCCTCAACGTCGGGGGGCTGGCGCACGAGGTACTCTGGCG
  TACCCTGGACCGCCTGCCCCGCACGCGGCTGGGCAAGCTCCGCGACTGCAACACGCACGACTCGCTGCTC
  GAGGTGTGCGATGACTACAGCCTCGACGACAACGAGTACTTCTTTGACCGCCACCCGGGCGCCTTCACCT
  CCATCCTCAACTTCTACCGCACTGGGCGACTGCACATGATGGAGGAGATGTGCGCGCTCAGCTTCAGCCA
  AGAGCTCGACTACTGGGGCATCGACGAGATCTACCTGGAGTCCTGCTGCCAGGCCCGCTACCACCAGAAG
  AAAGAGCAGATGAACGAGGAGCTCAAGCGTGAGGCCGAGACCCTACGGGAGCGGGAAGGCGAGGAGTTCG
  ATAACACGTGCTGCGCAGAGAAGAGGAAAAAACTCTGGGACCTACTGGAGAAGCCCAATTCCTCTGTGGC
  TGCCAAGATCCTTGCCATAATTTCCATCATGTTCATCGTCCTCTCCACCATTGCCCTGTCCCTCAACACG
  CTGCCTGAGCTACAGAGCCTCGATGAGTTCGGCCAGTCCACAGACAACCCCCAGCTGGCCCACGTGGAGG
  CCGTGTGCATCGCATGGTTCACCATGGAGTACCTGCTGAGGTTCCTCTCCTCGCCCAAGAAGTGGAAGTT
  CTTCAAGGGCCCACTCAATGCCATTGACTTGTTGGCCATTCTGCCATACTATGTCACCATTTTCCTCACC
  GAATCCAACAAGAGCGTGCTGCAATTCCAGAATGTCCGCCGCGTGGTCCAGATCTTCCGCATCATGCGAA
  TTCTCCGCATCCTTAAGCTTGCACGCCACTCCACTGGCCTCCAGTCTCTGGGCTTCACTTTGCGGAGGAG
  CTACAATGAGTTGGGCTTGCTCATCCTCTTCCTTGCCATGGGCATTATGATCTTCTCCAGCCTTGTCTTC
  TTTGCTGAGAAGGATGAGGACGACACCAAGTTCAAAAGCATCCCAGCCTCTTTCTGGTGGGCCACCATCA
  CCATGACTACTGTTGGGTATGGAGACATCTACCCCAAGACTCTCCTGGGGAAAATTGTTGGGGGACTCTG
  CTGCATTGCAGGAGTCCTGGTGATTGCTCTTCCCATCCCCATCATCGTCAATAACTTCTCTGAGTTCTAT
  AAGGAGCAGAAGAGACAGGAGAAAGCAATCAAACGGCGAGAGGCTCTGGAGAGAGCCAAGAGGAATGGCA
  GCATCGTATCCATGAACATGAAGGATGCTTTTGCCCGGAGCATTGAGATGATGGACATTGTGGTTGAGAA
  AAATGGGGAGAATATGGGTAAGAAAGACAAAGTACAAGATAACCACTTGTCTCCTAACAAATGGAAATGG
  ACAAAGAGGACACTGTCTGAAACCAGCTCAAGTAAGTCCTTTGAAACCAAGGAACAGGGATCCCCTGAAA
  AAGCCAGATCGTCTTCTAGTCCTCAGCACCTGAACGTTCAGCAGTTGGAAGACATGTACAATAAGATGGC
  CAAGACCCAATCCCAACCCATCCTCAATACCAAGGAGTCAGCAGCACAGAGCAAACCAAAGGAAGAACTT
  GAAATGGAGAGTATCCCCAGCCCCGTAGCCCCTCTGCCCACTCGCACAGAAGGGGTCATTGACATGCGAA
  GTATGTCAAGCATTGATAGTTTCATTAGCTGTGCCACAGACTTCCCTGAGGCCACCAGATTCTCCCACAG
  CCCTTTGACATCACTCCCCAGCAAGACTGGGGGCAGCACAGCCCCAGAAGTGGGCTGGCGGGGAGCTCTG
  GGTGCCAGTGGTGGTAGGTTTGTGGAGGCCAACCCCAGCCCTGATGCCAGCCAGCACTCTAGTTTCTTCA
  TCGAGAGCCCCAAGAGTTCCATGAAAACTAACAACCCTTTGAAGCTCCGAGCACTTAAAGTCAACTTCAT
  GGAGGGTGACCCCAGTCCACTCCTCCCCGTTCTAGGGATGTACCATGACCCTCTCAGGAACCGGGGGAGT
  GCTGCGGCTGCTGTCGCTGGACTGGAGTGTGCCACGCTTTTGGACAAGGCTGTGCTGAGCCCAGAGTCCT
  CCATCTACACCACAGCAAGTGCTAAGACACCCCCCCGGTCTCCTGAGAAACACACAGCAATAGCGTTCAA
  CTTTGAGGCGGGTGTCCACCAGTACATTGACGCAGACACAGATGATGAGGGACAGCTGCTCTACAGTGTG
  GACTCCAGCCCCCCCAAAAGCCTCCCTGGGAGCACCAGTCCGAAGTTCAGCACGGGGACAAGATCGGAGA
  AAAACCACTTTGAAAGCTCCCCTTTACCCACCTCCCCTAAGTTCTTAAGGCAGAACTGTATTTACTCCAC
  AGAAGCATTGACTGGAAAAGGCCCCAGTGGTCAGGAAAAGTGCAAACTTGAGAACCACATCTCCCCTGAC
  GTCCGTGTGTTGCCAGGGGGAGGAGCCCATGGAAGCACACGAGATCAGAGCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004975 unedited
  CTTCCGCACTAGGATCAGCGATGTTGGCGGGCATGACGTAAACATGAAATCCCGTTCCAC
  CAGCTCGCTGCCGCCCGAGCCCATGGAGATCGTGCGCAGCAAGGCGTGCTCTCGGCGGGT
  CCGCCTCAACGTCGGGGGGCTGGCGCACGAGGTACTCTGGCGTACCCTGGACCTGCGTCC
  CTTACGCGGTTTGGCAAGTTCCTCGACTGCAACATGCACGACTCGCTGCTCGAGGTGTGC
  GATGACTACAGCCTGGAGTACTACTAGTACTTCTTTGACCGCCACCCGGGCGCCTTCACC
  TCCATCCTCAACTTCTACCGCACTGGGCGACTGCACATGATGGAGGAGATGTGCGCGCTC
  AGCTTCAGCCAAGAGCTCGACTACTGGGGCATCGACGAGATCTACCTGGAGTCCTGCTGC
  CAGGCCCGCTACCACCAGAAGAAAGAGCAGATGAACGAGGAGCTCAAGCGTGAGGCCGAG
  ACCCTACGGGAGCGGGAAGGCGAGGAGTTCGATAACACGTGCTGCGCAGAGAAGAGGAAA
  AAACTCTGGGACCTACTGGAGAAGCCCAATTCCTCTGTGGCTGCCAAGATCCTTGCCATA
  ATTTCCATCATGTTCATCGTCCTCTCCACCATTGCCCTGTCCCTCAACACGCTGCCTGAG
  CTACAGAGCCTCGATGAGTTCGGCCAGTCCACAGAACACCCCCAGCTGGCCCACGTGGAG
  GCCGTGTGCATCGCATGGTTCACCATGGAGTACCTGCTGAGGTTCCTCTACTCGCCCAAG
  AAGTGGAAAGTCTTCAAAGGCCCCCTCAATGCCATTGGATTGTTGGCCATTCTGCCCTAC
  TATGTACCATTTTTCCTAACCGAATCCAACAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004975 unedited
  GGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAACTTGAAAACCCA
  NAAAACAAAACAGAATTTCTATTATAGTAGGTACTTTCCTTGCTGCAGCAGGAATTATTC
  AGTCTGAACTGGGCATTTCAATGCGTGGTATTTTTTTCCTTTCATTTTTGCAAGTAAAAA
  AATCATATTTTTATGCTGTTCTTCAGTAACACACAGTCTCTTTGAATAAACTATTTCTTT
  TGCATATTTGACACTTGCATATCTTTTGAGTCTACACTAGTGATGTAAAGAAAGCGTGTT
  CCTCCTCCAACCCTAAAACAATGCTCTGAGTTCATGCTGTTGGCTGGGTGCCCACCTTCT
  GAACCCTTCCCAGATTGCCAGGGGGCAATTAGAATTGAGTACAAGTGGTGCAACTCACCC
  TGATGGTATCTCAAAATAAAATCTCAGGTACTATGAAATACCGAAGTCAATGAAGACATT
  TGAGTAAGGGTTTTGCGCTGGGATCGTGGCTTATGGCGCAGAGACTTCCCCATGACCAAT
  CATTCCCTGTAGCTGTCTAACAGTGGAATCCATCCAGGAGGTGATGGACAGGTGTTCCTA
  GGGGTGGCTGGGCAACATTCAATACCAAATTGGTCAACCCACTCTTTCAACACCAGCCGC
  TTGTTCCAGCATCCTTCTCTAGCTGAAGCCAGNAGGACTGTCAATGTTGCCCTCCGAGGC
  TGAGATGGAAAGCCACGAGAGATGGGGAGTATGTGGCCCTCGTCCTGTCTAAAAGTGTGG
  TAGTGGTGGCTGTGAGTTCCACTCATGTCATCTCCCAAGAGGCTATAGAGTGTACAAGGA
  GTGCTGTGCCTATGCNNTGCCACTCATGCTGATGACTGTCATGCTCTAAGTCTANGGAGC
  CTCCTGAATCCTCTTTCTTGAGGCTTACGGCCTTCTGNTCCAGTCTGCAAAAACCTTNGC
  AGTAACTAACGTCTCTCTTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004975
• Insert Size: 3420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004975.2, NP_004966.1
• RefSeq Size: 3756 bp
• RefSeq ORF: 2577 bp
• Locus ID: 3745
• Cytogenetics: 20q13.13
• Domains: BTB, K_tetra, Kv2channel, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Protein Pathways: Taste transduction
• Gene Summary: 'Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shab-related subfamily. This member is a delayed rectifier potassium channel and its activity is modulated by some other family members. [provided by RefSeq, Jul 2008]'