CFAP410 (NM_004928) Human Untagged Clone

CAT#: SC117034

C21orf2 (untagged)-Human chromosome 21 open reading frame 2 (C21orf2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CFAP410
• Synonyms: C21orf2; LRRC76; RDMS; SMDAX; YF5/A2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117034 sequence for NM_004928 edited (data generated by NextGen Sequencing)
  ATGAAGCTGACGCGGAAGATGGTTCTGACCCGGGCCAAGGCCTCGGAGCTGCACAGCGTG
  CGCAAGCTCAACTGCTGGGGCAGCCGCCTCACAGATATCTCCATTTGCCAGGAGATGCCC
  AGCCTGGAGGTGATCACGCTCAGTGTCAACAGCATCTCCACCCTGGAGCCTGTGAGCCGG
  TGCCAGCGCCTGAGTGAGCTGTACCTGCGGAGGAACCGCATCCCCAGCCTGGCTGAGCTC
  TTCTACCTGAAGGGGCTGCCGCGTCTGCGGGTGCTGTGGCTGGCCGAGAACCCGTGCTGC
  GGCACCAGCCCCCACCGCTACCGCATGACCGTGCTGCGCACCCTGCCGCGCCTACAGAAG
  CTGGACAACCAGGCTGTGACGGAGGAGGAGCTGTCCCGTGCACTGAGTGAGGGAGAGGAG
  ATCACTGCGGCCCCAGAGAGAGAGGGCACAGGCCACGGCGGCCCCAAGCTATGCTGCACA
  CTGAGCTCCCTCAGCTCCGCTGCTGAGACTGGCCGGGACCCGCTGGACAGCGAGGAGGAG
  GCAACCNNCGGCGCCCAGGATGAACGTGGCCTGAAGCCGCCTTCCCGGGGCCAGTTTCCT
  TCCCTCTCAGCCAGGGATGCCTCGAGCAGCCACAGGGGCAGGAACGTCCTGACTGCCATC
  CTGCTGCTGCTGCGGGAGCTGGATGCAGAGGGGCTGGAGGCCGTGCAGCAGACTGTGGGC
  AGCCGGCTGCAGGCCCTGCGTGGGGAAGAGGTGCAGGAGCACGCCGAGTGA
  
  Clone variation with respect to NM_004928.2
  547 a=>n;548 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004928 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGCAGAGCCAACGTGTC
  AGCGGCGCCCAAGCGGCCCCAGCGGGCTCGCGTCGCCCCGCTCTCCTCACCGAGCCGCCA
  ATCGGCTCAGGATCCGCCCCTGACGACGCGGGCCCCGCCCCTGGAGACACGCGCCGCGCA
  GTCGTCACCCGCCCGGGATCAGGAGGCCGGGGGCGCCCGCCGGTCGGGCCTGGGCGGCCG
  CCATGAAGCTGACGCGGAAGATGGTTCTGACCCGGGCCAAGGCCTCGGAGCTGCACAGCG
  TGCGCAAGCTCAACTGCTGGGGCAGCCGCCTCACAGATATCTCCATTTGCCAGGAGATGC
  CCAGCCTGGAGGTGATCACGCTCAGTGTCAACAGCATCTCCACCCTGGAGCCTGTGAGCC
  GGTGCCAGCGCCTGAGTGAGCTGTACCTGCGGAGGAACCGCATCCCCAGCCTGGCTGAGC
  TCTTCTACCTGAAGGGGCTGCCGCGTCTGCGGGTGCTGTGGCTGGCCGAGAACCCGTGCT
  GCGGCACCAGCCCCCACCGCTACCGCATGACCGTGCTGCGCACCCTGCCGCGCCTACAGA
  AGCTGGACAACCAGGCTGTGACGGAGGAGGAGCTGTCCCGTGCACTGAGTGAGGGAGAGG
  AGATCACTGCGGCCCCGAGAGAGAGGCACAGGCCACGGCGGCCCCAGCTATGCTGCACAC
  TGAGCTCCCTCAGCTCCGCTGCTGAGACTGGCCCGGACCNGCTGGACAGCGAGGGAGGAG
  CACCCGCCGCCCAGATGAACGTGGCCTGAAGCCGCTTTCCCGGGCCAGTTTNCTTTCCTT
  TTAGCCAGGGATGCCTTGAGCAGCACAGGGGCACGGACGTCCTGACTGCATCCTGCTGCT
  GCTGCGGAACTGGAGCAANAGGGCTTGTAGGCCGGCACAAAACGGGGCCACCCGTTGAGC
  CCTGCTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004928 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAACCTTGGGAAAAATCTTTTA
  TTAGGGAGGACAGCCTGCAAAATCCTCCCTTTAAGAGCCCAGGCCAGCCTAGAACCTCCA
  GACCACAGAAGGGGAGTCCTGGGGACAGGACTGGTGTAGACAGGCATCTCCACCGCCCCG
  GTTAGCCAGTACCTAACACCCACTCCTGCCCTCGGCCGATGTGGCAAACCGGGGAGGCTT
  TTGTGTGGGGCCGGGGCTGCGGCCATGGCAGCCACCCTCCAGCTCCCGGGGGCTGGGGAA
  GACGCTGGGGTCCCCGTGGAGGCTGGAGCGGCGTTCAGGTCCTGCGGTCACTCGGCGTGC
  TCCTGCACCTCTTCCCCACGCAGGGCCTGCAGCCGGCTGCCCACAGTCTGCTGCACGGCC
  TCCAGCCCCTCTGCATCCAGCTCCCGCAGCAGCAGCAGGATGGCAGTCAGGACGTTCCTG
  CCCCTGTGGCTGCTCGAGGCATCCCTGGCTGAGAGGGAAGGAAACTGGCCCCGGGAAGGC
  GGCTTCAGGCCACGTTCATCCTGGGCGCCGGTTGCCTCCTCCTCGCTGTCCAGCGGGTCC
  CGGCCAGTCTCAGCAGCGGAGCTGAGGGAGCTCAGTGTGCAGCATAGCTTGGGGCCGCCG
  TGGCCTGTGCCCTCTCTCTCTGGGGCCGCAGTGATCTCCTCNTCCTCACTCAGTGCACGG
  GACAGCTCCTCCTNCGTCACAGCCTGGTTGTCCAGCTTCTGTANGCGCGGCAGGGTGCGC
  AGCACGGTCAATGCGTANCGGTGGGGGCTGTGCCGCANCACGGGTTCTCGGCCAGCCCAG
  CACCGCAGACGCGCAGCCCTTTCAGTATGAGAGCTCAGCAGCTGGGGATGCGGTCCTCCG
  CAGTACAGCTCACTCAGCGCTGGACCGGTTACAGCTCAGGGTGGAAAGCTGTTGCACTGA
  CCGGGACACCTCCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004928
• Insert Size: 1130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004928.1, NP_004919.1
• RefSeq Size: 2114 bp
• RefSeq ORF: 771 bp
• Locus ID: 755
• Cytogenetics: 21q22.3
• Domains: LRR, LRRcap
• Gene Summary: 'Four alternatively spliced transcript variants encoding four different isoforms have been found for this nuclear gene. All isoforms contain leucine-rich repeats. Three of these isoforms are mitochondrial proteins and one of them lacks the target peptide, so is not located in mitochondrion. This gene is down-regulated in Down syndrome (DS) brain, which may represent mitochondrial dysfunction in DS patients. [provided by RefSeq, Sep 2012]'
  Transcript Variant: This variant (1) encodes a mitochondrial protein (isoform 1).