CDK5 (NM_004935) Human Untagged Clone

CAT#: SC117039

CDK5 (untagged)-Human cyclin-dependent kinase 5 (CDK5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK5
• Synonyms: LIS7; PSSALRE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004935 edited
  ATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAG
  GCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGAT
  GATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCAC
  AAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTT
  GAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCT
  GAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGC
  AATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTG
  AAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCT
  GAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCC
  ACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGG
  CCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGG
  ACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCG
  ATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGG
  GATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCC
  CTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004935 unedited
  NGGTCAAATTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGCAGGGG
  TCCCCGCGGCCGCCGCGAGCAGAATACGAGAACTGGAAAAGATGGGGAAGGCACCTACGG
  AACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAG
  GCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAA
  GGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCT
  GACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGG
  TGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATT
  CTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAG
  GAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCG
  CTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGC
  CAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGC
  CAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTT
  CCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTA
  TAAGCCCTATCCGATGTACCCGGCCACACATCCCTGGTGAACGTCGTGCCCAAACTCATG
  CCACAGGNAGGGATCTGCTGCAGAACCTTCTGAGTGTAACCCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004935 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTGGGAAAGGA
  GCCAATTTATGAAATTAAATAAAGTCCACAAAGGGGAGTGGAGAAATTCGGNGCTCAGGG
  CACCCCACACCCGGCGTGGGGCCCAGCACCTGCTTGGAGCACAGACGCACCAGAGCACCC
  CCCACTTGTCTCACACCCTCTCAAGAGGGGGCCTTAAATAGGCCAGAGCCCCAGCCCTGG
  AGGCCGGGGGGTCCCGGGGNCCTAGGGGCGGACACGAAGTCGNGAGAAGTAGGGGTGCGT
  GCAGGGCCCTCTTCTGCTGAGATACGCTGGACAGGGTTACACTTCAGAAGGTTCTGCAGC
  AGATCCCTCCCTGTGGCATTGAGTTTGGGCACGACGTTCACCAGGGATGTTGTGGCCGGG
  TACATCGGATAGGGCTTATAGTCTGGCAGCTTGGTCATAGAGGGCCACTGCTCCTCGGTG
  GGCGTCCCCAGCAGTCGGAAGATCCTCTTCAACTGGTCATCGACATCATTGCCGGGAAAA
  AGAGGCCGCCCAGCATTGGCCAGCTCTGCAAAGATGCAGCCGGCTGACCACATGTCGATG
  GACGTGGAGTACAGCTTGGCCCCAAAGAGGACATCCGGTGGGCGGTACCACAGTGTGACC
  ACCTCAGCTGAGTAACAGCGGACGGGAATCCCAAGGCTCGAGCCAGGCCAAAATCAGCCA
  ATTTCAGCTCCCCATTCCTGCTTATTAGCCGGTTCCTGGGCCTCAGGTCCCTGTGTAGCC
  ACATGCGGCTATGACAGAATCCCACCCCTTTTAGTACCTGGAAGAGCATGACCTTACAAT
  CTCAGATCGAGGTCCCATTGCAACTGGCAAATACCTCTTCAGTCCCTGGTCCAGAATTCA
  AAACCAAGTCCCTTCTTGCCCTGTGCAGGACTCCTGAAGCTGCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004935
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004935.2, NP_004926.1
• RefSeq Size: 1143 bp
• RefSeq ORF: 879 bp
• Locus ID: 1020
• Cytogenetics: 7q36.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Alzheimer's disease, Axon guidance
• Gene Summary: 'This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.