CTNS (NM_004937) Human Untagged Clone

CAT#: SC117040

CTNS (untagged)-Human cystinosis, nephropathic (CTNS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTNS
• Synonyms: CTNS-LSB; PQLC4; SLC66A4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117040 sequence for NM_004937 edited (data generated by NextGen Sequencing)
  ATGATAAGGAATTGGCTGACTATTTTTATCCTTTTTCCCCTGAAGCTCGTAGAGAAATGT
  GAGTCAAGCGTCAGCCTCACTGTTCCTCCTGTCGTAAAGCTGGAGAACGGCAGCTCGACC
  AACGTCAGCCTCACCCTGCGGCCACCATTAAATGCAACCCTGGTGATCACTTTTGAAATC
  ACATTTCGTTCCAAAAATATTACTATCCTTGAGCTCCCCGATGAAGTTGTGGTGCCTCCT
  GGAGTGACAAACTCCTCTTTTCAAGTGACATCTCAAAATGTTGGACAACTTACTGTTTAT
  CTACATGGAAATCACTCCAATCAGACCGGCCCGAGGATACGCTTTCTTGTGATCCGCAGC
  AGCGCCATTAGCATCATAAACCAGGTGATTGGCTGGATCTACTTTGTGGCCTGGTCCATC
  TCCTTCTACCCTCAGGTGATCATGAATTGGAGGCGGAAAAGTGTCATTGGTCTGAGCTTC
  GACTTCGTGGCTCTGAACCTGACGGGCTTCGTGGCCTACAGTGTATTCAACATCGGCCTC
  CTCTGGGTGCCCTACATCAAGGAGCAGTTTCTCCTCAAATACCCCAACGGAGTGAACCCC
  GTGAACAGCAACGACGTCTTCTTCAGCCTGCACGCGGTTGTCCTCACGCTGATCATCATC
  GTGCAGTGCTGCCTGTATGAGCGCGGTGGCCAGCGCGTGTCCTGGCCTGCCATCGGCTTC
  CTGGTGCTCGCGTGGCTCTTCGCATTTGTCACCATGATCGTGGCTGCAGTGGGAGTGATC
  ACGTGGCTGCAGTTTCTCTTCTGCTTCTCCTACATCAAGCTCGCAGTCACGCTGGTCAAG
  TATTTTCCACAGGCCTACATGAACTTTTACTACAAAAGCACTGAGGGCTGGAGCATTGGC
  AACGTGCTCCTGGACTTCACCGGGGGCAGCTTCAGCCTCCTGCAGATGTTCCTCCAGTCC
  TACAACAACGACCAGTGGACGCTGATCTTCGGAGACCCAACCAAGTTTGGACTCGGGGTC
  TTCTCCATCGTCTTCGACGTCGTCTTCTTCATCCAGCACTTCTGTTTGTACAGAAAGAGA
  CCGGGGTATGACCAGCTGAACTAG
  
  Clone variation with respect to NM_004937.2
  779 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004937 unedited
  TGGAGCACCAGGGAGGCGGTGCATTCCTGACCGGCACCTGGCGAGGCTCATGCGTCCCGT
  GAGGGCGGTTCCTCGAGCCTGGGGGCGCTCAGATTGCTTTGGAGACGCTGAGAGAACCTT
  TGCGAGAGCGCCGGTTGACGTGCGGAGTGCGGGGCTCCGGGGGACTGAGCAGCACGAGAC
  CCCATCCTCCCCTCCGGGTTTTCACACTGGGCGAAGGGAGGACTCCTGAGCTCTGCCTCT
  TCCAGTAACATTGAGGATTACTGTGTTTTGTGAGAGCTCGCTAGGCGCCCTAAGCAACAG
  AGTTCTGAGAAATCGAGAAACATGATAAGGAATTGGCTGACTATTTTTATCCTTTTTCCC
  CTGAAGCTCGTAGAGAAATGTGAGTCAAGCGTCAGCCTCACTGTTCCTCCTGTCGTAAAG
  CTGGAGAACGGCAGCTCGACCAACGTCAGCCTCACCCTGCGGCCACCATTAAATGCAACC
  CTGGTGATCACTTTTGAAATCACATTTCGTTCCAAAAATATTACTATCCTTGAGCTCCCC
  GATGAAGTTGTGGTGCCTCCTGGAGTGACAAACTCCTCTTTTCAAGTGACATCTCAAAAT
  GTTGGACAACTTACTGTTTATCTACATGGAAATCACTCCAATCAGACCGGCCCGAGGATA
  CGCTTTCTTGTGATCCGCAGCAGCGCCATTAGCATCATANACCAGGTGATTGGCTGGATC
  TACTTTGTGGCCTGGTCCATCTNCTTCTACCCTCAGGTGATCATGAATTTGGAGCNGAAA
  GTGTCATTGGTCTGAGCTTCGACTTCGTGGCTCTGAACCTGACCGGCTTCGTGGCCTACA
  GTGTATTNCACATCGGCCTCCTCTGGGTAGCCTACTCAAGGAGCAGTTCCTCCTAAATAC
  CCAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004937 unedited
  TTTTTAGAAAGGAAAGCGAGTAGGTTTTAATTCAAAAACAGGCCCCTCGCGTTGATCTCG
  TAAAAGGAAACTCAGGGGACTGACAAGCTCAAGTCATGTATGAGGCACGTCCTGGGACCC
  CCACCCCTCCTGCCATAGGAAGGACAGCTTTGGGCAAAGGGAAGGAGGTTTGAAATCAGG
  GTTGGCCCCATACAAATTGGGTGAGGGGGTCTCAAGTACAAATACTTATCTGAGGCTCCT
  GAACAGGCCAAAAATTGGTGAGTCTCAAGTAGGTGTCTGGGGAAAAAGAGGGAAGGGGCC
  TGCCCTCGCTCCAGGGGAGCTGGTCGCCGTTTGGCAGGCCTAACAAACCTCTAAGGCACA
  AACTGGTAGCAGGAAAAAGCTATGTCCTGTACTCCAAATGCTGGGTAAGGAGCAGCTGGA
  TGTGCTCAAATGGGGCTCTTCTGAAAAGGTGGAGGTAGGAAAAAGGGCAAAAAAGAGTAA
  GCCACTTCCAAACGTCCCTCCAAAAAATCTGAAAGGAGTTACTATGCTAATGCGCTGGGC
  ATGAATCCAATTCAAAACCAATCGTGGTGGGCCCCTCATGGCTAAAAAATACTCTGGGTA
  GGGGCTTGGCTGTTACTGCAGTCACGGACGCTGAGTTATGTACAGAGAGTACGTCAGGGA
  TAGTGGCCTTAAGAAGAAGAGCCCCATGGCCTAACCCCAAAGTCCTAGCCCGTCCACCAG
  CACCTNTTGAGTTCACTGGCCACGCACCCGGCTGTTTGATCTTTTGGAATGCACCGGTGG
  CAACAAAGTGCCCTTGGCTCACCCGGTTGAAAGGTAGCCCTTAAGCTGCTGCAAAACGCT
  GGGGNNTTGGGGGCAAAGCCCTTTGTTGAAACCTGCTGGCCATCCTGNCTGAAAACGGTG
  CCAAACCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004937
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004937.1, NP_004928.1
• RefSeq Size: 2611 bp
• RefSeq ORF: 1104 bp
• Locus ID: 1497
• Cytogenetics: 17p13.2
• Domains: CTNS
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: 'This gene encodes a seven-transmembrane domain protein that functions to transport cystine out of lysosomes. Its activity is driven by the H+ electrochemical gradient of the lysosomal membrane. Mutations in this gene cause cystinosis, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR and 3' coding region, compared to variant 1, resulting in an isoform (2) with a distinct and shorter C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.