MARK2 (NM_004954) Human Untagged Clone

CAT#: SC117049

MARK2 (untagged)-Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK2
• Synonyms: EMK-1; EMK1; PAR-1; Par-1b; Par1b
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004954 edited
  GAATTCGGCACGAGGCCATGTCCAGCGCTCGGACCCCCCTACCCACGCTGAACGAGAGGG
  ACACGGAGCAGCCCACCTTGGGACACCTTGACTCCAAGCCCAGCAGTAAGTCCAACATGA
  TTCGGGGCCGCAACTCAGCCACCTCTGCTGATGAGCAGCCCCACATTGGAAACTACCGGC
  TCCTCAAGACCATTGGCAAGGGTAATTTTGCCAAGGTGAAGTTGGCCCGACACATCCTGA
  CTGGGAAAGAGGTAGCTGTGAAGATCATTGACAAGACTCAACTGAACTCCTCCAGCCTCC
  AGAAACTATTCCGCGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTTAAAT
  TATTTGAAGTGATTGAGACTGAGAAAACGCTCTACCTTGTCATGGAGTACGCTAGTGGCG
  GAGAGGTATTTGATTACCTAGTGGCTCATGGCAGGATGAAAGAAAAAGAGGCTCGAGCCA
  AATTCCGCCAGATAGTGTCTGCTGTGCAGTACTGTCACCAGAAGTTTATTGTCCATAGAG
  ACTTAAAGGCAGAAAACCTGCTCTTGGATGCTGATATGAACATCAAGATTGCAGACTTTG
  GCTTCAGCAATGAATTCACCTTTGGGAACAAGCTGGACACCTTCTGTGGCAGTCCCCCTT
  ATGCTGCCCCAGAACTCTTCCAGGGCAAAAAATATGATGGACCCGAGGTGGATGTGTGGA
  GCCTAGGAGTTATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAGAACC
  TCAAGGAGCTGCGGGAACGGGTACTGAGGGGAAAATACCGTATTCCATTCTACATGTCCA
  CGGACTGTGAAAACCTGCTTAAGAAATTTCTCATTCTTAATCCCAGCAAGAGAGGCACTT
  TAGAGCAAATCATGAAAGATCGATGGATGAATGTGGGTCACGAAGATGATGAACTAAAGC
  CTTACGTGGAGCCACTCCCTGACTACAAGGACCCCCGGCGGACAGAGCTGATGGTGTCCA
  TGGGTTATACACGGGAAGAGATCCAGGACTCGCTGGTGGGCCAGAGATACAACGAGGTGA
  TGGCCACCTATCTGCTCCTGGGCTACAAGAGCTCCGAGCTGGAAGGCGACACCATCACCC
  TGAAACCCCGGCCTTCAGCTGATCTGACCAATAGCAGCGCCCCATCCCCATCCCACAAGG
  TACAGCGCAGCGTGTCGGCCAATCCCAAGCAGCGGCGCTTCAGCGACCAGGCTGGTCCTG
  CCATTCCCACCTCTAATTCTTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAATAAGC
  GGCCTGAGGAGGACCGGGAGTCAGGGCGGAAAGCCAGCAGCACAGCCAAGGTGCCTGCCA
  GCCCCCTGCCCGGTCTGGAGAGGAAGAAGACCACCCCAACCCCCTCCACGAACAGCGTCC
  TCTCCACCAGCACAAATCGAAGCAGGAATTCCCCACTTTTGGAGCGGGCCAGCCTCGGCC
  AGGCCTCCATCCAGAATGGCAAAGACAGCCTAACCATGCCAGGGTCCCGGGCCTCCACGG
  CTTCTGCTTCTGCCGCAGTCTCTGCGGCCCGGCCCCGCCAGCACCAGAAATCCATGTCGG
  CCTCCGTGCACCCCAACAAGGCCTCTGGGCTGCCCCCCACGGAGAGTAACTGTGAGGTGC
  CGCGGCCCAGCACAGCCCCCCAGCGTGTCCCTGTTGCCTCCCCATCCGCCCACAACATCA
  GCAGCAGTGGTGGAGCCCCAGACCGAACTAACTTCCCCCGGGGTGTGTCCAGCCGAAGCA
  CCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAGAATTTGCCCTACGGTGTGA
  CCCCAGCCTCTCCCTCTGGCCACAGCCAGGGCCGGCGGGGGGCCTCTGGGAGCATCTTCA
  GCAAGTTCACCTCCAAGTTTGTACGCAGGAACCTGAATGAACCTGAAAGCAAAGACCGAG
  TGGAGACGCTCAGACCTCACGTGGTGGGCAGTGGCGGCAACGACAAAGAAAAGGAAGAAT
  TTCGGGAGGCCAAGCCCCGCTCCCTCCGCTTCACGTGGAGTATGAAGACCACGAGCTCCA
  TGGAGCCCAACGAGATGATGCGGGAGATCCGCAAGGTGCTGGACGCGAACAGCTGCCAGA
  GCGAGCTGCATGAGAAGTACATGCTGCTGTGCATGCACGGCACGCCGGGCCACGAGGACT
  TCGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCGCGGCTCTCTCTCAACGGGGTTCGAT
  TTAAGCGGATATCGGGCACCTCCATGGCCTTCAAAAACATTGCCTCCAAAATAGCCAACG
  AGCTGAAGCTTTAACAGGCTGCCAGGAGCGGGGGCGGCGGGGGCGGGCCAGCTGGACGGG
  CTGCCGGCCGCTGCGCCGCCCCACCTGGGCGAGACTGCAGCGATGGATTGGTGTGTCTCC
  CCTGCTGGCACTTCTCCCCTCCCTGGCCCTTCTCAGTTTTCTCTTACATGTTTGTGGGGG
  GTGGGAXXXXXXXXXXXXXXXXXXXXXXXCCACATTCACCCCTGCCCAGAGATTCCCCCT
  TCTCCTCTCCCCTACTGGAGGCAAAGGAAGGGGAGGGTGGATGGGGGGGCAGGGCTCCCC
  CTCGGTACTGCGGTTGCACAGAGTATTTCGCCTAAACCAAGAAATTTTTTATTACCAAAA
  AGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004954 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCATGTCCAGCGCTCGGACCC
  CCCTACCCACGCTGAACGAGAGGGACACGGAGCAGCCCACCTTGGGACACCTTGACTCCA
  AGCCCAGCAGTAAGTCCAACATGATTCGGGGCCGCAACTCAGCCACCTCTGCTGATGAGC
  AGCCCCACATTGGAAACTACCGGCTCCTCAAGACCATTGGCAAGGGTAATTTTGCCAAGG
  TGAAGTTGGCCCGACACATCCTGACTGGGAAAGAGGTAGCTGTGAAGATCATTGACAAGA
  CTCAACTGAACTCCTCCAGCCTCCAGAAACTATTCCGCGAAGTAAGAATAATGAAGGTTT
  TGAATCATCCCAACATAGTTAAATTATTTGAAGTGATTGAGACTGAGAAAACGCTCTACC
  TTGTCATGGAGTACGCTAGTGGCGGAGAGGTATTTGATTACCTAGTGGCTCATGGCAGGA
  TGAAAGAAAAAGAGGCTCGAGCCAAATTCCGCCAGATAGTGTCTGCTGTGCAGTACTGTC
  ACCAGAAGTTTATTGTCCATAGAGACTTAAAGGCAGAAAACCTGCTCTTGGATGCTGATA
  TGAACATCAAGATTGCAGACTTTGGCTTCAGCAATGAATTCACCTTTGGGAACAAGCTGG
  ACACCTTCTGTGGCAGTCCCCCTTATGCTGCCCCAGATCTCTTCCAGGGCATAAAATATG
  ATGGACCCGAGGTGGATGTGTGGAGCCTANNGAGTATCCTCTATACACTGGTCAGCGGAT
  CCCTGCCTTTTGATGGACAGAACCCTCAGGAGCTGCGGNAACGGGTACTGAGGNGAAAAT
  ACCGTATTTCATTCTACATGTCCACGGACTGTGAANACCCTGCTAANNGAATTCTCATTC
  TTATCCCCAGCAGAGAGCACTCTAGAGCAATCATGGAAGATCGATGGATGAATGTGGGTC
  ACGAANATGATGAACTAAGACCTACGTGGAGCCCTNCCTGACTACAGGACCCCGCNGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004954 unedited
  CAACCGCAGTACCGAGGGGGAGCCCTGCCCCCCCATCCACCCTCCCCTTCCTTTGCCTCC
  AGTAGGGGAGAGGAGAAGGGGGAATCTCTGGGCAGGGGTGAATGTGGGGGGCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004954
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004954.2, NP_004945.2
• RefSeq Size: 2784 bp
• RefSeq ORF: 2076 bp
• Locus ID: 2011
• Cytogenetics: 11q13.1
• Domains: UBA, pkinase, KA1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]'
  Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region and uses an alternate in-frame splice site in the central coding region, compared to variant 4. The resulting isoform (c) lacks two internal segments and 1 internal residue, compared to isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.