HDAC1 (NM_004964) Human Untagged Clone

CAT#: SC117054

HDAC1 (untagged)-Human histone deacetylase 1 (HDAC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HDAC1
• Synonyms: GON-10; HD1; KDAC1; RPD3; RPD3L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004964 edited
  ATGGCGCAGACGCAGGGCACCCGGAGGAAAGTCTGTTACTACTACGACGGGGATGTTGGA
  AATTACTATTATGGACAAGGCCACCCAATGAAGCCTCACCGAATCCGCATGACTCATAAT
  TTGCTGCTCAACTATGGTCTCTACCGAAAAATGGAAATCTATCGCCCTCACAAAGCCAAT
  GCTGAGGAGATGACCAAGTACCACAGCGATGACTACATTAAATTCTTGCGCTCCATCCGT
  CCAGATAACATGTCGGAGTACAGCAAGCAGATGCAGAGATTCAACGTTGGTGAGGACTGT
  CCAGTATTCGATGGCCTGTTTGAGTTCTGTCAGTTGTCTACTGGTGGTTCTGTGGCAAGT
  GCTGTGAAACTTAATAAGCAGCAGACGGACATCGCTGTGAATTGGGCTGGGGGCCTGCAC
  CATGCAAAGAAGTCCGAGGCATCTGGCTTCTGTTACGTCAATGATATCGTCTTGGCCATC
  CTGGAACTGCTAAAGTATCACCAGAGGGTGCTGTACATTGACATTGATATTCACCATGGT
  GACGGCGTGGAAGAGGCCTTCTACACCACGGACCGGGTCATGACTGTGTCCTTTCATAAG
  TATGGAGAGTACTTCCCAGGAACTGGGGACCTACGGGATATCGGGGCTGGCAAAGGCAAG
  TATTATGCTGTTAACTACCCGCTCCGAGACGGGATTGATGACGAGTCCTATGAGGCCATT
  TTCAAGCCGGTCATGTCCAAAGTAATGGAGATGTTCCAGCCTAGTGCGGTGGTCTTACAG
  TGTGGCTCAGACTCCCTATCTGGGGATCGGTTAGGTTGCTTCAATCTAACTATCAAAGGA
  CACGCCAAGTGTGTGGAATTTGTCAAGAGCTTTAACCTGCCTATGCTGATGCTGGGAGGC
  GGTGGTTACACCATTCGTAACGTTGCCCGGTGCTGGACATATGAGACAGCTGTGGCCCTG
  GATACGGAGATCCCTAATGAGCTTCCATACAATGACTACTTTGAATACTTTGGACCAGAT
  TTCAAGCTCCACATCAGTCCTTCCAATATGACTAACCAGAACACGAATGAGTACCTGGAG
  AAGATCAAACAGCGACTGTTTGAGAACCTTAGAATGCTGCCGCACGCACCTGGGGTCCAA
  ATGCAGGCGATTCCTGAGGACGCCATCCCTGAGGAGAGTGGCGATGAGGACGAAGACGAC
  CCTGACAAGCGCATCTCGATCTGCTCCTCTGACAAACGAATTGCCTGTGAGGAAGAGTTC
  TCCGATTCTGAAGAGGAGGGAGAGGGGGGCCGCAAGAACTCTTCCAACTTCAAAAAAGCC
  AAGAGAGTCAAAACAGAGGATGAAAAAGAGAAAGACCCAGAGGAGAAGAAAGAAGTCACC
  GAAGAGGAGAAAACCAAGGAGGAGAAGCCAGAAGCCAAAGGGGTCAAGGAGGAGGTCAAG
  TTGGCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004964 unedited
  TTTAGNATTTGGCTACTATCATATAGGCGGCCCGCGATTCGGCACGAGGCTGACGGTAGG
  GACGGGAGGCGAGCAAGATGGCGCAGACGCAGGGCACCCGGAGGAAAGTCTGTTACTACT
  ACGACGGGGATGTTGGAAATTACTATTATGGACAAGGCCACCCAATGAAGCCTCACCGAA
  TCCGCATGACTCATAATTTGCTGCTCAACTATGGTCTCTACCGAAAAATGGAAATCTATC
  GCCCTCACAAAGCCAATGCTGAGGAGATGACCAAGTACCACAGCGATGACTACATTAAAT
  TCTTGCGCTCCATCCGTCCAGATAACATGTCGGAGTACAGCAAGCAGATGCAGAGATTCA
  ACGTTGGTGAGGACTGTCCAGTATTCGATGGCCTGTTTGAGTTCTGTCAGTTGTCTACTG
  GTGGTTCTGTGGCAAGTGCTGTGAAACTTAATAAGCAGCAGACGGACATCGCTGTGAATT
  GGGCTGGGGGCCTGCACCATGCAAAGAAGTCCGAGGCATCTGGCTTCTGTTACGTCAATG
  ATATCGTCTTGGCCATCCTGGAACTGCTAAAGTATCACCAGAGGGTGCTGTACATTGACA
  TTGATATTCACCATGGTGACGGCGTGGAAGAGGCCTTCTACACCACGGACCGGGTCATGA
  CTGTGTCCTTTCATAAGTATGGAGAGTACTTCCCAGGAACTGGGGACCTACGGGGATATC
  GGGCTGGGCAAGCAAGTATTATGCTGTTAACTACCCGCTCCGAGACCGGATTGATGACGA
  GTCCTATGAGGCCATTTCCAAGCCGTCATGTCCAAGGAATGGNAGAGTTCCACCCTAGTG
  CGGTGGGTCTACAGGGTGGCTCAGACTCCTATCTGGGGATCGGGTANGTTGCTTCATCTA
  ACTTCAAAGACCCGCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004964 unedited
  CAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAGAAAGGGACCATTTTAT
  TACAAAGAGGCTCACAAAAATGAAAATAGTATCTCAAAAAGGAAACTAGACTAGCAACCT
  CCACCTGCAGAATTAGGAGAAGACAGACAGAGGGCAGGCAGTGTTTCTTGGCTCACTTGA
  GGCCAGTGGGAAGGTACTAATACAGAACCAATCTAAAAATGGCTGATGTTACTTTAGGAG
  CCTGAAAAAAACAGGAGATCCTTGAAGACCCAGCCACCCCTTCTAGAATGTTCAATAAGG
  GCACCTTTCCAAAGCTACTAAGCAGGCACTTGGCATTTCAGGAGTTTGTCTTATGGTTGC
  ATAAAAGTATCCCTTTCACCCAGACCTGGCACCCTTTATGGTTCAAAGTTAAGAACGGGA
  AGAATGGGTGGCAAGGTGGCTCCTGGAAGAGCTCACCCAGCACAGCTGCCCTGAGCTCGG
  GGCCTTGGTTTCTGTCCCTGGGGATATTTATATTTAATAAATTTTTATATAAATTCCCCG
  AGAACTAGAAAATATAAAATCTGAGGGGTTGGGGAAGAAACGTGAGGGACTCACCAGGAA
  GCCAGAGCTGGAGAAGTCCATTCAGGCCAACTTGACCTCCTCCTTGACCCCTTTGGCTTC
  TGGACTTCCCCTCCCTGGGTTTTTTCCTTCGGGAGACCTCCTTTTTCTCCTCTGGGCCTT
  TTCTTTTTTTATCCCCGGTTTGGACTCTTTTGGGCTTTTTTGAAGTTGGAACAATTTTTG
  CGGGCCCCCTTTTCTCCTTTTTATAATCGGAAAATCTTCTCTACAGGCATCCCTTTTCAA
  AAACACCCACCAAGTCTTGCCAGGTGCCCTTCCCCCCCACCCCTTCCTTAGGTACGGCGC
  CCATGATTTCCGCTTTGTCCCCCCCGTCGCGGATTTTTATGGCCCCAAACCGCCGCTTCC
  CCCCTGTCCATCCCCGCCGTTTCACTTATATACCCGCCCCCCCCCACTTCCATTCCCCCC
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004964
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004964.2, NP_004955.2
• RefSeq Size: 2091 bp
• RefSeq ORF: 1449 bp
• Locus ID: 3065
• Cytogenetics: 1p35.2-p35.1
• Domains: Hist_deacetyl
• Protein Families: Adult stem cells, Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer
• Gene Summary: 'Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]'