PERK (EIF2AK3) (NM_004836) Human Untagged Clone

CAT#: SC117132

EIF2AK3 (untagged)-Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2AK3
• Synonyms: PEK; PERK; WRS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004836 edited
  ATGGAGCGCGCCATCAGCCCGGGGCTGCTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTG
  CTGGGGCTCGCGGCAAGGACGGTGGCCGCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACG
  GCGGAGGCGGCGTTCGGCCTCGGGGCGGCCGCTGCTCCCACCTCAGCGACGCGAGTACCG
  GCGGCGGGCGCCGTGGCTGCGGCCGAGGTGACTGTGGAGGACGCTGAGGCGCTGCCGGCA
  GCCGCGGGAGAGCAGGAGCCTCGGGGTCCGGAACCAGACGATGAGACAGAGTTGCGACCG
  CGCGGCAGGTCATTAGTAATTATCAGCACTTTAGATGGGAGAATTGCTGCCTTGGATCCT
  GAAAATCATGGTAAAAAGCAGTGGGATTTGGATGTGGGATCCGGTTCCTTGGTGTCATCC
  AGCCTTAGCAAACCAGAGGTATTTGGGAATAAGATGATCATTCCTTCCCTGGATGGAGCC
  CTCTTCCAGTGGGACCGAGACCGTGAAAGCATGGAAACAGTTCCTTTCACAGTTGAATCA
  CTTCTTGAATCTTCTTATAAATTTGGAGATGATGTTGTTTTGGTTGGAGGAAAATCTCTG
  ACTACATATGGACTCAGTGCATATAGTGGAAAGGTGAGGTATATCTGTTCAGCTCTGGGT
  TGTCGCCAATGGGATAGTGACGAAATGGAACAAGAGGAAGACATCCTGCTTCTACAGCGT
  ACCCAAAAAACTGTTAGAGCTGTCGGACCTCGCAGTGGCAATGAGAAGTGGAATTTCAGT
  GTTGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACGAGAGCCGGATTTATTGAA
  AGCACCTTTAAGCCCAATGAGAACACAGAAGAGTCTAAAATTATTTCAGATGTGGAAGAA
  CAGGAAGCTGCCATAATGGACATAGTGATAAAGGTTTCGGTTGCTGACTGGAAAGTTATG
  GCATTCAGTAAGAAGGGAGGACATCTGGAATGGGAGTACCAGTTTTGTACTCCAATTGCA
  TCTGCCTGGTTACTTAAGGATGGGAAAGTCATTCCCATCAGTCTTTTTGATGATACAAGT
  TATACATCTAATGATGATGTTTTAGAAGATGAAGAAGACATTGTAGAAGCTGCCAGAGGA
  GCCACAGAAAACAGTGTTTACTTGGGAATGTATAGAGGCCAGCTGTATCTGCAGTCATCA
  GTCAGAATTTCAGAAAAGTTTCCTTCAAGTCCCAAGGCTTTGGAATCTGTCACTAATGAA
  AACGCAATTATTCCTTTACCAACAATCAAATGGAAACCCTTAATTCATTCTCCTTCCAGA
  ACTCCTGTCTTGGTAGGATCTGATGAATTTGACAAATGTCTCAGTAATGATAAGTTTTCT
  CATGAAGAATATAGTAATGGTGCACTTTCAATCTTGCAGTATCCATATGATAATGGTTAT
  TATCTACCATACTACAAGAGGGAGAGGAACAAACGAAGCACACAGATTACAGTCAGATTC
  CTCGACAACCCACATTACAACAAGAATATCCGCAAAAAGGATCCTGTTCTTCTTTTACAC
  TGGTGGAAAGAAATAGTTGCAACGATTTTGTTTTGTATCATAGCAACAACGTTTATTGTG
  CGCAGGCTTTTCCATCCTCATCCTCACAGGCAAAGGAAGGAGTCTGAAACTCAGTGTCAA
  ACTGAAAATAAATATGATTCTGTAAGTGGTGAAGCCAATGACAGTAGCTGGAATGACATA
  AAAAACTCTGGATATATATCACGATATCTAACTGATTTTGAGCCAATTCAATGCCTGGGA
  CGTGGTGGCTTTGGAGTTGTTTTTGAAGCTAAAAACAAAGTAGATGACTGCAATTATGCT
  ATCAAGAGGATCCGTCTCCCCAATAGGGAATTGGCTCGGGAAAAGGTAATGCGAGAAGTT
  AAAGCCTTAGCCAAGCTTGAACACCCGGGCATTGTTAGATATTTCAATGCCTGGCTCGAA
  GCACCACCAGAGAAGTGGCAAGAAAAGATGGATGAAATTTGGCTGAAAGATGAAAGCACA
  GACTGGCCACTCAGCTCTCCTAGCCCAATGGATGCACCATCAGTTAAAATACGCAGAATG
  GATCCTTTCTCTACAAAAGAACATATTGAAATCATAGCTCCTTCACCACAAAGAAGCAGG
  TCTTTTTCAGTAGGGATTTCCTGTGACCAGACAAGTTCATCTGAGAGCCAGTTCTCACCA
  CTGGAATTCTCAGGAATGGACCATGAGGACATCAGTGAGTCAGTGGATGCAGCATACAAC
  CTCCAGGACAGTTGCCTTACAGACTGTGATGTGGAAGATGGGACTATGGATGGCAATGAT
  GAGGGGCACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTCCTTATGTAAGGTCAAGGGAG
  AGAACCTCCTCTTCAATAGTATTTGAAGATTCTGGCTGTGATAATGCTTCCAGTAAAGAA
  GAGCCGAAAACTAATCGATTGCATATTGGCAACCATTGTGCTAATAAACTAACTGCTTTC
  AAGCCCACCAGTAGCAAATCTTCTTCTGAAGCTACATTGTCTATTTCTCCTCCAAGACCA
  ACCACTTTAAGTTTAGATCTCACTAAAAACACCACAGAAAAACTCCAGCCCAGTTCACCA
  AAGGTGTATCTTTACATTCAAATGCAGCTGTGCAGAAAAGAAAACCTCAAAGACTGGATG
  AATGGACGATGTACCATAGAGGAGAGAGAGAGGAGCGTGTGTCTGCACATCTTCCTGCAG
  ATCGCAGAGGCAGTGGAGTTTCTTCACAGTAAAGGACTGATGCACAGGGACCTCAAGCCA
  TCCAACATATTCTTTACAATGGATGATGTGGTCAAGGTTGGAGACTTTGGGTTAGTGACT
  GCAATGGACCAGGATGAGGAAGAGCAGACGGTTCTGACCCCAATGCCAGCTTATGCCAGA
  CACACAGGACAAGTAGGGACCAAACTGTATATGAGCCCAGAGCAGATTCATGGAAACAGC
  TATTCTCATAAAGTGGACATCTTTTCTTTAGGCCTGATTCTATTTGAATTGCTGTATCCA
  TTCAGCACTCAGATGGAGAGAGTCAGGACCTTAACTGATGTAAGAAATCTCAAATTTCCA
  CCATTATTTACTCAGAAATATCCTTGTGAGTACGTGATGGTTCAAGACATGCTCTCTCCA
  TCCCCCATGGAACGACCTGAAGCTATAAACATCATTGAAAATGCTGTATTTGAGGACTTG
  GACTTTCCAGGAAAAACAGTGCTCAGACAGAGGTCTCGCTCCTTGAGTTCATCGGGAACA
  AAACATTCAAGACAGTCCAACAACTCCCATAGCCCTTTGCCAAGCAATTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004836 unedited
  GCAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCGGGCT
  GAGACGTGGCCAGGGGAACACGGCTGGCTGTCCAGGCCGTCGGGGCGGCAGTAGGGTCCC
  TAGCACGTCCTTGCCTTCTTGGGAGCTCCAAGCGGCGGGAGAGGCAGGCGTCAGTGGCTG
  CGCCTCCATGCCTGCGCGCGGGGCGGGACGCTGATGGAGCGCGCCATCAGCCCGGGGCTG
  CTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTGCTGGGGCTCGCGGCAAGGACGGTGGCC
  GCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACGGCGGAGGCGGCGTTCGGCCTCGGGGCG
  GCCGCTGCTCCCACCTCAGCGACGCGAGTACCGGCGGCGGGCGCCGTGGCTGCGGCCGAG
  GTGACTGTGGAGGACGCTGAGGCGCTGCCGGCAGCCGCGGGAGAGCAGGAGCCTCGGGGT
  CCGGAACCAGACGATGAGACAGAGTTGCGACCGCGCGGCAGGTCATTAGTAATTATCAGC
  ACTTTAGATGGGAGAATTGCTGCCTTGGATCCTGAAAATCATGGTAAAAAGCAGTGGGAT
  TTGGATGTGGGATCCGGTTCCTTGGTGTCATCCAGCCTTAGCAAACCAGAGGTATTTGGG
  AATAAGATGATCATTCCTTCCCTGGATGGAGCCCTCTTCCAGTGGGACCGAGACCGTGAA
  AGCATGGAAACAGTTCCTTTCACAGTTGAATCACTTCTTGAATCTTCTTTATAATTTGGA
  GATGATGTTGTTTTGGTTGGGAGGAAATCTCTGACTACATATGGACTCAGTGCATATAGT
  GGAAAGGTGAGGTATATCTGTTCAGCTCTGGGGTGTCGCCATGGGATAGTGACGAATGGG
  ACCAGAGGAAGACTNNCTGCTCTACGCGTACCCAANAACTGTTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004836 unedited
  GTGGACGAAGCCGCCTGNNAGATCGAGTTTTTTTCTTTTTTTTTTTTTTAATTAAAATTA
  GTTTTATTAAATCATTTAAACTTGAAAAAATCACAAGTTAACACACTTAAGTGTGTTCTG
  TACACCACCAACCCAAATGGATTGATTCCACAATTTTTATAAATAAAACATAAACATATT
  TACAGTGAAAAATAAAAACATGTCAGCAAGCGATATGATTAAACTGTTACCTATAATAAA
  TTTGGGGGAAGTACCAACCTACCGGATTACGTGTCCTATACTTACCATTCCATATCGTTT
  CCCAATCCCCTCCGACCCTAAACCCCCCCCCCCCAGTACGTAGGCATCCCTTACTCCCAA
  TTCTCTTCATCCAATCTTTATTCCCCTCACATCTCCAATCAACACTCTATTCTCTTCCCA
  TCTTACCCCCACTTTTCTTCTTTTGTTTTCATTTCTTTCTAGTCTACCCCCCCTCCTACT
  CCCTCCCACCCCTCTTCATCTTTCCTTCTTCCAATCCCCTCCGTGTTTTTCCCCCTTCCC
  CTTTTAATTCTTTCCACCTTTTTCTTCCCCCTCTCCGCCCCCATTTTTAACTCCCCTATT
  TTTCCCTCCTTTTACACCCCCCCTGTTTTTCCAGCCCCACCCCCCCCTTTTTTTTTCTAT
  TTTTTATCCCACCCCCCTCCTATCCCCCCTTTTTCCCTTTCTCCCTATCCCCCCCCATTT
  TTATTTTTTCTCCTTCCCCCCCCCCTTTTTTTCCTTCCCTTCCCCCCTCCTCCTTCTCCC
  CTACTATTTCTTATATCCTCCACCCCCTTTCTCTCTTCCCCCCCCCCCACCCCTCCCCTT
  TTTTTCCCCCTCCCCCCCTTTCCCCATTCCCCCCCACCCTTCCCGTCCTCTTACCTAATA
  TCATCACACATTCCCCCACCGCTCACCCCCCCCCTTTTTTGTTTTTCCTCCCCCCTTTCA
  CCCCCCCGCCTCTATTTCACCCACCTCACTACCTAATTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004836
• ORF Size: 3351 bp
• Insert Size: 4280
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_004836.3, NP_004827.3
• RefSeq Size: 4511
• RefSeq ORF: 3351
• Locus ID: 9451
• Domains: pkinase, TyrKc, S_TKc, PQQ
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
• Protein Pathways: Alzheimer's disease
• Gene Summary: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.