LARGE (NM_004737) Human Untagged Clone

CAT#: SC117185

LARGE (untagged)-Human like-glycosyltransferase (LARGE), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LARGE
• Synonyms: LARGE; MDC1D; MDDGA6; MDDGB6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004737, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGGAATCTGCAGGGGGAGACGGAAATTCTTGGCTGCCTCGTTGAGTCTTCTCTGCATCCCAGCCA
  TCACCTGGATTTACCTGTTTTCTGGGAGCTTCGAAGATGGAAAGCCCGTGTCTCTGTCACCGCTGGAGTC
  CCAGGCACACAGCCCCAGGTACACGGCCTCCAGCCAGCGGGAGCGCGAGAGCCTGGAGGTGCGCATGCGC
  GAGGTGGAGGAGGAGAACCGCGCCCTCCGCAGGCAGCTCAGCCTGGCCCAGGGCCGAGCCCCATCCCATC
  GCCGAGGCAACCACTCCAAGACCTACTCCATGGAGGAGGGCACTGGAGACAGCGAGAACCTTCGGGCTGG
  CATCGTGGCAGGCAACAGCTCCGAGTGTGGGCAGCAGCCGGTCGTGGAGAAATGCGAGACAATCCACGTT
  GCTATTGTCTGCGCCGGATACAATGCCAGCCGGGATGTCGTCACCCTGGTCAAATCCGTCCTGTTCCATA
  GACGGAACCCTCTGCACTTCCACCTTATTGCTGACTCCATTGCGGAGCAGATCCTGGCCACGCTCTTCCA
  GACCTGGATGGTGCCCGCTGTGCGTGTGGACTTCTACAATGCAGACGAGCTCAAGTCTGAAGTTTCCTGG
  ATCCCCAATAAACATTACTCTGGGATTTATGGTCTGATGAAGCTTGTCCTGACCAAGACTCTTCCTGCCA
  ACCTGGAGAGAGTCATCGTCCTTGACACGGATATCACCTTTGCCACTGACATTGCAGAGCTGTGGGCTGT
  GTTCCACAAGTTCAAAGGTCAGCAAGTCCTGGGCTTGGTGGAGAACCAGAGTGACTGGTACCTTGGAAAC
  CTGTGGAAAAATCACCGCCCATGGCCAGCCCTTGGAAGAGGCTACAACACAGGGGTGATCCTGTTACTTC
  TGGATAAGCTGCGGAAGATGAAATGGGAGCAGATGTGGAGGCTGACCGCAGAGAGGGAGCTCATGGGCAT
  GCTCTCTACATCCTTAGCTGACCAGGATATTTTCAATGCCGTCATCAAACAAAACCCCTTCCTTGTGTAC
  CAGCTCCCCTGCTTCTGGAATGTGCAGCTGTCAGACCACACCCGCTCCGAGCAGTGCTACAGAGACGTGT
  CTGATCTAAAGGTCATTCACTGGAACTCCCCCAAGAAGCTCCGGGTGAAGAACAAGCATGTGGAGTTTTT
  TCGCAACCTCTACCTGACCTTCCTGGAGTATGACGGCAATCTTCTGAGGCGGGAACTGTTTGGCTGCCCC
  AGTGAGGCTGATGTCAACAGTGAAAACCTCCAGAAGCAGCTGTCTGAGCTGGACGAGGACGACCTGTGCT
  ATGAGTTCCGGCGAGAGCGCTTCACTGTCCACCGCACCCACCTGTACTTCCTGCACTACGAGTATGAGCC
  TGCAGCAGACAGCACGGACGTCACCCTGGTCGCTCAGCTGTCCATGGACAGGCTCCAGATGCTGGAGGCC
  ATCTGCAAGCACTGGGAGGGGCCCATCAGCCTGGCCCTCTACCTGTCAGACGCCGAGGCCCAGCAGTTCC
  TCCGCTACGCACAGGGCTCTGAGGTGCTTATGAGCCGCCACAACGTGGGCTACCACATCGTGTACAAGGA
  GGGCCAGTTCTACCCCGTGAACCTGCTGCGCAACGTGGCCATGAAGCACATCAGCACTCCCTACATGTTC
  CTGTCTGACATTGACTTCCTGCCCATGTATGGGCTCTATGAGTACCTCAGGAAGTCTGTCATCCAGCTCG
  ATCTTGCCAACACCAAGAAAGCAATGATTGTCCCCGCGTTCGAGACACTGCGCTACCGGCTGTCCTTCCC
  CAAGTCAAAAGCGGAGTTGCTGTCAATGCTGGACATGGGGACCCTCTTCACATTCAGGTACCACGTCTGG
  ACGAAAGGCCACGCACCCACAAACTTCGCCAAGTGGCGGACCGCCACCACGCCTTACCGGGTTGAGTGGG
  AGGCCGATTTTGAGCCGTATGTTGTTGTGAGACGTGACTGCCCGGAGTACGACCGGAGGTTTGTAGGCTT
  TGGCTGGAACAAAGTGGCTCATATCATGGAGCTGGATGTGCAGGAGTATGAGTTCATTGTGCTGCCCAAC
  GCCTACATGATCCACATGCCTCATGCCCCCAGCTTCGACATTACCAAGTTCCGTTCCAACAAGCAATACC
  GCATCTGTCTCAAAACCCTCAAGGAAGAGTTTCAGCAGGACATGTCCCGCCGCTACGGCTTTGCTGCCCT
  GAAATATCTCACAGCCGAGAACAACAGCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004737 unedited
  NGGTCCAAATTTTGNTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCTGG
  CTCGGAAGAGTCCCCCGGGCCGGGAGCAGCTCCAGGCAGCGGCCCCGGAGGAAGAGGAAG
  AAGGGACAGTGCTCAGCTTGGGGGACCCGGACCCTCGCCGCGGCATTTGGAGCCGGGGGC
  AGTCCCGAACTCTGTGCTTGGCACCGCCGCTCCGAGTAGGGCAGCGCCTGCCGGGACCCT
  GACCCGGACCCCCTGCGCCTCGTAGGCGGCGGCGCCGCCGCGCCACCCTGTTCTTCCGTG
  TCTCCCTCTGCCTGGCGGCAGTCACGGCCAAGAGAGTATTATGAGGGAGGCCGAGGACTT
  CATGCTCCGGACAGAGAAACGGCGCTGGGATTAGGGATTGCCACTTCTGAGAGGATGCTG
  GGAATCTGCAGGGGGAGACGGAAATTCTTGGCTGCCTCGTTGAGTCTTCTCTGCATCCCA
  GCCATCACCTGGATTTACCTGTTTTCTGGGAGCTTCGAAGATGGAAAGCCCGTGTCTCTG
  TCACCGCTGGAGTCCCAGGCACACAGCCCCAGGTACACGGCCTCCAGCCAGCGGGAGCGC
  GAGAGCCTGGAGGTGCGCATGCGCGAGGTGGAGGAGGAGAACCGCGCCCTCCGCAGGCAG
  CTCAGCCTGGCCCAGGGCCGAGCCCCATCCCATCGCCGAGGCAACCACTCCAAGACCTAC
  TCCATGGAGGANGGCACTGGAGACAGCGAGAACCTTCGGGCTGGCATCGTGGCAGGCAAC
  AGCTCCGAGTGTGGGCAGCAGCCCGNTCGTGGAGAAATGCGAGACAAATCCACGTGCTAT
  TGTCTGCGCTGGATCAATGCCAGCCGGGATGTCGTCACCTGGTCAATCCCGTCTGTCCAT
  AGACGNA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004737 unedited
  NNNAAAATTTAGCTATGNCCGCGGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTG
  TCTGTACTTTCATTTATTAAATGCAGCTTTCGCTGGTCACATTGCAACTCAATACAAACA
  GCCCAGCAGAGGGTAGGGCATTAACTCTTGTTCTCATCAATGTAAACACAATATTAATAT
  TGAAGATTAAAGAAAATGGGTAAAAAGTCCAGTGTCTCCTCAATCTGTGTGAAGGTGAAG
  GTGGTTGCCTACCCTTGGACAGGTCCCAAAGGGAGACTGAGGTTGTCACCTCTTCCCATC
  CACAGTGAAGCAGGCAAGAACCAGAGGAACCCAATCACTTTCTTCCTGTAGGTCTCCAGA
  TGGATACGTGAATCTTCAGAACTGGGCTTTCATGAATTATGAAAGTTCTTCGAAAGGCCT
  GAGAGGTTCGTACCTTGTGTGTGCACTGAAGTAGTTCCTTCAAAGCCCTTTCCCATGAAT
  CAGTCACTGCCAATANAAAATGTGACCGGTGTAAAACAGCCAGCCCTCGTAATTCCGCAG
  TCCCCATCGCTATAGCCCCTGGATTCTGGAGAGTAGGGAGTTCAAAGTCACGGGAGCCTC
  CGTGATCATCCTGAAGGAAGCTGCCCATGTTAAATATCGGGCGAAGATGCTTGACCTCCT
  TCCTGGGCCACTGCTGAATGAGGNGGGGTTGGTAAAAGCAANNTAATTTTTCTTTATAAC
  CCTCTAAGATCAGATTTTTTATTTTGGATTGGCACCCCCAAAATAAACAAACCCCCCAGA
  AAAAACAAACAAAACAGGAGTGACTTTTGGGATAAAGGAAACCATTACCCCCCCTCCCGG
  AACCCTGGGTTCAAGGCCCCATTGGGTTCTTCCAAAAGGGGGGGTTAAAAAATAAGTATG
  CCCTCCGTTGGAAACCCAACTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004737
• ORF Size: 2271 bp
• Insert Size: 4110
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_004737.3, NP_004728.1
• RefSeq Size: 4194
• RefSeq ORF: 2271
• Locus ID: 9215
• Domains: Glyco_transf_8
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. The protein encoded by this gene is the glycotransferase that adds the final xylose and glucuronic acid to alpha-dystroglycan and thereby allows alpha-dystroglycan to bind ligands including laminin 211 and neurexin. Mutations in this gene cause several forms of congenital muscular dystrophy characterized by cognitive disability and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2018]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.