Translin (TSN) (NM_004622) Human Untagged Clone

CAT#: SC117228

TSN (untagged)-Human translin (TSN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSN
• Synonyms: BCLF-1; C3PO; RCHF1; REHF-1; TBRBP; TRSLN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117228 sequence for NM_004622 edited (data generated by NextGen Sequencing)
  ATGTCTGTGAGCGAGATCTTCGTGGAGCTGCAGGGCTTTTTGGCTGCCGAGCAGGACATC
  CGAGAGGAAATCAGAAAAGTTGTACAGAGTTTAGAACAAACAGCTCGAGAGATTTTAACT
  CTACTGCAAGGGGTCCATCAGGGTGCTGGGTTTCAGGACATTCCAAAGAGGTGTTTGAAA
  GCTCGAGAACATTTTGGTACAGTAAAAACACATCTAACATCTTTGAAGACCAAATTTCCT
  GCTGAACAGTATTACAGATTTCATGAGCACTGGAGGTTTGTGTTGCAGCGCTTGGTCTTC
  TTGGCAGCATTTGTTGTGTATTTGGAAACAGAAACACTAGTGACTCGAGAAGCAGTTACA
  GAAATTCTTGGCATTGAGCCAGATCGGGAGAAAGGATTTCATCTGGATGTAGAAGATTAT
  CTCTCAGGAGTTCTAATTCTTGCCAGTGAACTGTCGAGGCTGTCTGTCAACAGCGTGACT
  GCTGGAGACTACTCCCGACCCCTCCACATCTCCACCTTCATCAATGAGCTGGATTCCGGT
  TTTCGCCTTCTCAACCTGAAAAATGACTCCCTGAGGAAGCGCTACGACGGATTGAAATAT
  GACGTGAAGAAAGTAGAGGAAGTGGTCTATGATCTCTCCATCCGGGGCTTTAATAAGGAG
  ACGGCAGCAGCTTGTGTTGAAAAATAG
  
  Clone variation with respect to NM_004622.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004622 unedited
  NGGTCGAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCGGTAGCGG
  CGGCCGTTGCGATTGATTGCGCTGGTTGCCTGCGGCGTCCACTTCCTTGGCCGCCCTTGC
  TACACTGGCTGATTGTTGTGCAGCCGGCGCCATGTCTGTGAGCGAGATCTTCGTGGAGCT
  GCAGGGCTTTTTGGCTGCCGAGCAGGACATCCGAGAGGAAATCAGAAAAGTTGTACAGAG
  TTTAGAACAAACAGCTCGAGAGATTTTAACTCTACTGCAAGGGGTCCATCAGGGTGCTGG
  GTTTCAGGACATTCCAAAGAGGTGTTTGAAAGCTCGAGAACATTTTGGTACAGTAAAAAC
  ACATCTAACATCTTTGAAGACCAAATTTCCTGCTGAACAGTATTACAGATTTCATGAGCA
  CTGGAGGTTTGTGTTGCAGCGCTTGGTCTTCTTGGCAGCATTTGTTGTGTATTTGGAAAC
  AGAAACACTAGTGACTCGAGAAGCAGTTACAGAAATTCTTGGCATTGAGCCAGATCGGGA
  GAAAGGATTTCATCTGGATGTAGAAGATTATCTCTCAGGAGTTCTAATTCTTGCCAGTGA
  ACTGTCGAGGCTGTCTGTCAACAGCGTGACTGCTGGAGACTACTCCCGACCCCTCCACAT
  CTCCACCTTCATCAATGAGCTGGATTCCGGTTNTCGCCTTCTCAACCTGAAAAATGACTC
  CCTGAGGAAGCGCTACGACGGATTGAAATATGACGTGAAGAAAGTAGAGGAAGTGGTCTA
  TGATCTCTCCATCCGGNGCTTTAATANGAGACGGCAGCAGCTTGTGTTGAAAAATAGAGG
  CTCTCCTTGCTCCTGGCCTTGCTGACCTCAGCGGGTGCCAGAAAGGGGTGAGCACAGAGT
  GCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004622 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAAAAAAACCAAAAAA
  ATTTTATTGCTTTACAACAAGCCAGCAACAAACACAACTGAATAGTTATAAACATGACAC
  TTCTAAATCCTCAAATGGAATTCCAAGACCAGAGTGTGCTTCTCCACTTTAATAGCATTT
  GTTAAAACTGAAATCCACAACTTCTGGCAAGTACTTCAATATTCTCAAGCAGAATCAATA
  CTTAAAGAGGTTAAAAGAAAAAACTATTATCAAACGTATTGTCTGTGGGATGATCTAATT
  TAAAAGCCTTTCATGCTTGATCCTGGCAGTGCTATTACTGCACATTTTAAGTGACCATCA
  TGGGCACTTTTAAGAACCTAACTTCACAAAAAGAGCTGTCACAAGAAAATTGTTCTAAAA
  TCCCAATGCACAGTAACTCATGCCTCATTTCAACTGCTTTCCTCATGATGAAAAAATGTA
  GTCAGCCTTTAACTTGGCCTGATAAAAGCTGTGAAAGGTATGCCAAATGGCCCCTTGTAC
  AAACAGGAAGCCCAAATTGTTGATCAGGCAAGAAAAGGTGACACTTTTAGATACTTCATA
  TTATTCTGGAAAGCACTTTCCTTTCAGTGGGTGCAAAATAACTACGGATTAAGTCACCAT
  TTTAATCATCTCAATTCAATAGCAAGAGTTTCCTGGCACATACTCTTAGTACTACCTGAA
  AATTGGGGCGTGTGGGGAAGACCACCAACAGTATTTACATGACTTGCCCCAAACTGTACA
  TTTAAAACATGACTGGGTGGATAGTCACCAACACCTGAAATTCTTACTCTAAACACATTA
  CCAATCCCACGATTATTTTGGNGTGCTTCGGGNNTTTGTTTTAATAGAATGAGTCTACTT
  GTGCCAGCTGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004622
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004622.2, NP_004613.1
• RefSeq Size: 3408 bp
• RefSeq ORF: 687 bp
• Locus ID: 7247
• Cytogenetics: 2q14.3
• Domains: Translin
• Gene Summary: 'This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. This protein encoded by this gene, when complexed with translin-associated protein X, also forms a Mg ion-dependent endoribonuclease that promotes RNA-induced silencing complex (RISC) activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).