XPC (NM_004628) Human Untagged Clone

CAT#: SC117234

XPC (untagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XPC
• Synonyms: p125; RAD4; XP3; XPCC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117234 sequence for NM_004628 edited (data generated by NextGen Sequencing)
  ATGGCTCGGAAACGCGCGGCCGGCGGGGAGCCGCGGGGACGCGAACTGCGCAGCCAGAAA
  TCCAAGGCCAAGAGCAAGGCCCGGCGTGAGGAGGAGGAGGAGGATGCCTTTGAAGATGAG
  AAACCCCCAAAGAAGAGCCTTCTCTCCAAAGTTTCACAAGGAAAGAGGAAAAGAGGCTGC
  AGTCATCCTGGGGGTTCAGCAGATGGTCCAGCAAAAAAGAAAGTGGCCAAGGTGACTGTT
  AAATCTGAAAACCTCAAGGTTATAAAGGATGAAGCCCTCAGCGATGGGGATGACCTCAGG
  GACTTTCCAAGTGACCTCAAGAAGGCACACCATCTGAAGAGAGGGGCTACCATGAATGAA
  GACAGCAATGAAGAAGAGGAAGAAAGTGAAAATGATTGGGAAGAGGTTGAAGAACTTAGT
  GAGCCTGTGCTGGGTGACGTGAGAGAAAGTACAGCCTTCTCTCGATCTCTTCTGCCTGTG
  AAGCCAGTGGAGATAGAGATTGAAACGCCAGAGCAGGCGAAGACAAGAGAAAGAAGTGAA
  AAGATAAAACTGGAGTTTGAGACATATCTTCGGAGGGCGATGAAACGTTTCAATAAAGGG
  GTCCATGAGGACACACACAAGGTTCACCTTCTCTGCCTGCTAGCAAATGGCTTCTATCGA
  AATAACATCTGCAGCCAGCCAGATCTGCATGCTATTGGCCTGTCCATCATCCCAGCCCGC
  TTTACCAGAGTGCTGCCTCGAGATGTGGACACCTACTACCTCTCAAACCTGGTGAAGTGG
  TTCATTGGAACATTTACAGTTAATGCAGAACTTTCAGCCAGTGAACAAGATAACCTGCAG
  ACTACATTGGAAAGGAGATTTGCTATTTACTCTGCTCGAGATGATGAGGAATTGGTCCAT
  ATATTCTTACTGATTCTCCGGGCTCTGCAGCTCTTGACCCGGCTGGTATTGTCTCTACAG
  CCAATTCCTCTGAAGTCAGCAACAGCAAAGGGAAAGAAACCTTCCAAGGAAAGATTGACT
  GCGGATCCAGGAGGCTCCTCAGAAACTTCCAGCCAAGTTCTAGAAAACCACACCAAACCA
  AAGACCAGCAAAGGAACCAAACAAGAGGAAACCTTTGCTAAGGGCACCTGCAGGCCAAGT
  GCCAAAGGGAAGAGGAACAAGGGAGGCAGAAAGAAACGGAGCAAGCCCTCCTCCAGCGAG
  GAAGATGAGGGCCCAGGAGACAAGCAGGAGAAGGCAACCCAGCGACGTCCGCATGGCCGG
  GAGCGGCGGGTGGCCTCCAGGGTGTCTTATAAAGAGGAGAGTGGGAGTGATGAGGCTGGC
  AGCGGCTCTGATTTTGAGCTCTCCAGTGGAGAAGCCTCTGATCCCTCTGATGAGGATTCC
  GAACCTGGCCCTCCAAAGCAGAGGAAAGCCCCCGCTCCTCAGAGGACAAAGGCTGGGTCC
  AAGAGTGCCTCCAGGACCCATCGTGGGAGCCATCGTAAGGACCCAAGCTTGCCAGCGGCA
  TCCTCAAGCTCTTCAAGCAGTAAAAGAGGCAAGAAAATGTGCAGCGATGGTGAGAAGGCA
  GAAAAAAGAAGCATAGCTGGTATAGACCAGTGGCTAGAGGTGTTCTGTGAGCAGGAGGAA
  AAGTGGGTATGTGTAGACTGTGTGCACGGTGTGGTGGGCCAGCCTCTGACCTGTTACAAG
  TACGCCACCAAGCCCATGACCTATGTGGTGGGCATTGACAGTGACGGCTGGGTCCGAGAT
  GTCACACAGAGGTACGACCCAGTCTGGATGACAGTGACCCGCAAGTGCCGGGTTGATGCT
  GAGTGGTGGGCCGAGACCTTGAGACCATACCAGAGCCCATTTATGGACAGGGAGAAGAAA
  GAAGACTTGGAGTTTCAGGCAAAACACATGGACCAGCCTTTGCCCACTGCCATTGGCTTA
  TATAAGAACCACCCTCTGTATGCCCTGAAGCGGCATCTCCTGAAATATGAGGCCATCTAT
  CCCGAGACAGCTGCCATCCTTGGGTATTGTCGTGGAGAAGCGGTCTACTCCAGGGATTGT
  GTGCACACTCTGCATTCCAGAGACACGTGGCTGAAGAAAGCAAGAGTGGTGAGGCTTGGA
  GAAGTACCCTACAAGATGGTGAAAGGCTTTTCTAACCGTGCTCGGAAAGCCCGACTTGCT
  GAGCCCCAGCTGCGGGAAGAAAATGACCTGGGCCTGTTTGGCTACTGGCAGACAGAGGAG
  TATCAGCCCCCAGTGGCCGTGGACGGGAAGGTGCCCCGGAACGAGTTTGGGAATGTGTAC
  CTCTTCCTGCCCAGCATGATGCCTATTGGCTGTGTCCAGCTGAACCTGCCCAATCTACAC
  CGCGTGGCCCGCAAGCTGGACATCGACTGTGTCCAGGCCATCACTGGCTTTGATTTCCAT
  GGCGGCTACTCCCATCCCGTGACTGATGGATACATCGTCTGCGAGGAATTCAAAGACGTG
  CTCCTGACTGCCTGGGAAAATGAGCAGGCAGTCATTGAAAGGAAGGAGAAGGAGAAAAAG
  GAGAAGCGGGCTCTAGGGAACTGGAAGTTGCTGGCCAAAGGTCTGCTCATCAGGGAGAGG
  CTGAAGCGTCGCTACGGGCCCAAGAGTGAGGCAGCAGCTCCCCACACAGATGCAGGAGGT
  GGACTCTCTTCTGATGAAGAGGAGGGGACCAGCTCTCAAGCAGAAGCGGCCAGGATACTG
  GCTGCCTCCTGGCCTCAAAACCGAGAAGATGAAGAAAAGCAGAAGCTGAAGGGTGGGCCC
  AAGAAGACCAAAAGGGAAAAGAAAGCAGCAGCTTCCCACCTGTTCCCATTTGAGAAGCTG
  TGA
  
  Clone variation with respect to NM_004628.4
  2061 g=>a;2815 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004628 unedited
  AATCCGGCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGA
  ATTCGGCACGAGGCCCAGACAAGCAACATGGCTCGGAAACGCGCGGCCGGCGGGGAGCCG
  CGGGGACGCGAACTGCGCAGCCAGAAATCCAAGGCCAAGAGCAAGGCCCGGCGTGAGGAG
  GAGGAGGAGGATGCCTTTGAAGATGAGAAACCCCCAAAGAAGAGCCTTCTCTCCAAAGTT
  TCACAAGGAAAGAGGAAAAGAGGCTGCAGTCATCCTGGGGGTTCAGCAGATGGTCCAGCA
  AAAAAGAAAGTGGCCAAGGTGACTGTTAAATCTGAAAACCTCAAGGTTATAAAGGATGAA
  GCCCTCAGCGATGGGGATGACCTCAGGGACTTTCCAAGTGACCTCAAGAAGGCACACCAT
  CTGAAGAGAGGGGCTACCATGAATGAAGACAGCAATGAAGAAGAGGAAGAAAGTGAAAAT
  GATTGGGAAGAGGTTGAAGAACTTAGTGAGCCTGTGCTGGGTGACGTGAGAGAAAGTACA
  GCCTTCTCTCGATCTCTTCTGCCTGTGAAGCCAGTGGAGATAGAGATTGAAACGCCAGAG
  CAGGCGAAGACCAGAGGAAGAAGTGAAAAGATAAAACTGGAGTTGGAGACATATCTTCGG
  AGGGCGATGAAACGTTTCATAAAGGGGTCCATGAGGACACACCACAAGTTACCTTTCTTG
  GCTGCTAGCAAAGGGGTTCTATCGAATAACATCTGCAGCCAGCCAGATCTGCATGCTATT
  GGCCTGGCCATTATCCCAGCCCGTTTTCACAGATGCTGCCTCGAGAGGTGAACACCTATA
  CCCTTAAACCGGGGGAAGGGTTCATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004628 unedited
  GGTAATCTATGNACGCGGCCGCATTCTAGATCGAGTTTTTTTTTTTTTTTTTTTTTAGAT
  AAATGCTTTATTATTTTCTCAAAATGTTATAAAAGTCATTTCTCCTTAGTACAGAGAGCT
  TTATACATTTTATCTAGTAATGAATACAATCTAGTTTAACACTGATCTATTTTACTAACA
  TTTCCTTAATTTTCAATTCGTATTTTTCCTTTTCTTTCCTGATTTTAGCTTTTTTTAGGC
  TTCTGTCACCACAAAATGTTACTTGGAAAACTAGATCCCAGCAGATGACCTGTACTTCTC
  TGCTCTCTCCCCTACTGCAAAGAGGCAGTGAGGGCAGCATTAGTAAGCCTGACTCAGGGG
  AAGGTAAGTGGCCTGCAGAGAAATGGTCCTAGGTCCGCAACCGAGGCGAGTGAACTTGTC
  GGACAGATGAAGACTCTAACTGGAAGAAACGATCAGCGCATTCACGGATGCACCACCATC
  CAGAAATCTCCCGGTGGCTTCCATACAAAAACTGATGTTTCTAAAGATGGAAAGAACAGG
  TCTAGGAGGCAGAAGAGTATCTCCTAGCAAAGTGTTCTGTAGCTCAAAGGGTGAGTGGGC
  TTTGGTAGCAAAAAGCTTTGAAGGCTTCACCCTGGGTGAATCTGACAAGGGCTGGAGCCA
  GACGGGACCTGCAGCACCTCCTCAGCTTGGCCTCGTCTCCCCTGACCCCGCCTCCGTGCA
  TGCTGCCTCAGTTTGCCTTCTCAGCAGAGAAGCCCCCACCACCAGGGGCTGGGCATGCCC
  AGGGCAGGTGTGGGGCCTGTAGTGGGGCAGCAGCAACTGGTGGGGTGCCCCTCTAGTGGG
  CGCTCAGCTCACAGCTTCTCAAATGGGGACCAGGTGGGAAGCTGCTGNCTTTCTTTTTCC
  TTTTGGGTCTTCTTGGGGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004628
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004628.3, NP_004619.2
• RefSeq Size: 3714 bp
• RefSeq ORF: 2823 bp
• Locus ID: 7508
• Cytogenetics: 3p25.1
• Domains: Rad4
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• Gene Summary: 'The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).