BAP1 (NM_004656) Human Untagged Clone

CAT#: SC117256

BAP1 (untagged)-Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAP1
• Synonyms: hucep-6; HUCEP-13; UCHL2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004656, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTTCACCCTGCTCGTGGAAGATTTCGGTG
  TCAAGGGGGTGCAAGTGGAGGAGATCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTAT
  CTTCCTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTTGGTGGATGATACGTCC
  GTGATTGATGATGATATTGTGAATAACATGTTCTTTGCCCACCAGCTGATACCCAACTCTTGTGCAACTC
  ATGCCTTGCTGAGCGTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCATGAAGGA
  CTTCACCAAGGGTTTCAGCCCTGAGAGCAAAGGATATGCGATTGGCAATGCCCCGGAGTTGGCCAAGGCC
  CATAATAGCCATGCCAGGCCCGAGCCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGA
  CCATGGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTGAGCTGGATGGGCTGAA
  GGTCTACCCCATTGACCATGGGCCCTGGGGGGAGGACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATC
  ATGGAGCGTATCGGCCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGGCAGTGG
  TGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTGAAGGTGAACCGTCAGACAGTACTAGA
  GGCTCTGCAGCAGCTGATAAGAGTAACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAG
  CTGCCTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCAAACAGGGCCCCTGCAG
  CCTCTGAGGGCAACCACACAGATGGTGCAGAGGAGGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAG
  CCCTCCCAACAAACCCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCCAACCCC
  ACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAATTATGCCAAGTCCCCCATGCAGGAGG
  AAGAAGACCTGGCGGCAGGTGTGGGCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGA
  TGAGGATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGCCCTTAGGTATAAGGGG
  AAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTTCTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACA
  CCATCAACGTCTTGGCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCATCAAGAC
  TAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACTCGCAGCCCTCACCCACCCCCAGCAAT
  GAGAGTACAGACACGGCCTCTGAGATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAG
  CCAACCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTTTTGGAGAGGATGACAG
  CCTGCTGCGTGTTGACTGCATACGCTACAACCGTGCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGC
  CTGCTGCACCTGGCTGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTTCCTCGC
  CCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCAGTGGAGAAGGAGGTCGTGGAAGCCAC
  GGACAGCAGAGAGAAGACGGGGATGGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAG
  GAGCTGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCGTGCCTCAAGGAGGAGG
  TAGAGAAGAGGAAGAAGTTCAAGATTGATGACCAGAGAAGGACCCACAACTACGATGAGTTCATCTGCAC
  CTTTATCTCCATGCTGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTGCGGCGG
  CGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCCTGACCGGCGGAAACGCTCTCGCCCCT
  ACAAGGCCAAGCGCCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004656 unedited
  CCCCCCTATCTCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGGGACTGAGGGGCCCCGGGGGCGGTGGGGGCTCCCGGTGGG
  GGCAGCGGTGGGGAGGGAGGGCCTGGACATGGCGCTGAGGGGCCGCCCCGCGGGAAGATG
  AATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTTCACCCTGCTCGTGGAAGAT
  TTCGGTGTCAAGGGGGTGCAAGTGGAGGAGATCTACGACCTTCAGAGCAAATGTCAGGGC
  CCTGTATATGGATTTATCTTCCTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAG
  GTCTCTACCTTGGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTC
  TTTGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGCGTGCTCCTG
  AACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCATGAAGGACTTCACCAAGGGT
  TTCAGCCCTGAGGCCCGAGCCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGT
  GCGGACCATGGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTATGA
  GCTGGATGGGCTGCAAGTCTACCCCATTGACCATGGGCCCTGGGGGGCAGACGAAGAGTG
  CACAGACCAAGCCCTGGCGGTCATCATGAAGCGTATCGGCCTCGCCACTGCAGGGGAGCC
  CTACCACGACATCCGCTTCAACCTGATGGCAGTGTTGCCCGACCGCAGGACCAAGTATGA
  GGCCTAGCTGCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004656 unedited
  CAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCACGA
  AACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTCTTATCTGGGCCCTGACATTTGCT
  CTGAAGGTCGTAGATCTCCTCCACTTGCACCACCTCGACCGCGATTCTTAGGAGAGCTTG
  ATGCATTCGCTGGTCCCGTTTTTCCAGGGCCTACAGGGGTCAAGCTGTGCTCACCCCAAA
  GGCAGTTGCCACACTTGCCAGCCCCCCCCACTCAACCACTATGTCCACATAAAGGGGCCT
  GCTTGGATCACCTTTTTCAAAGCCATCTGGCCGAAGCCATGGGCCTTGGTTGGGGCCCGG
  GCTCCAAGGGCACTGTTGGGCCCATTACCCGTTGTCAGCAAGTCCTACGGAACACCGGGG
  CCCCAAACGGTCGATTTGCTCCTGCTGTCCCCCACCATGACTGCCTTGCAGAGGCTGCGG
  AAACTGGTGTTGCACGAACAGCCTACTGTTGCCATCTTCCTATAAGAGACCTTGTCTCTG
  CTCCCCGTGAGAGGGAGACATGGTCACACTGATGTGCTGGACAGAGGCTCTTATGATCCT
  AATGGCCCTGGCTCAGACACCTATGTCCCAATTTAGGCCCATAGCTCGGGCCGCCACGTT
  CAGGGCAAAGTGCCCTAGAACCTTGTTACGGAGAGCAGCCTTGATCAGCCTGGGACTAAC
  AAGAAAAAACGTTCAGAGGCCTGTGAACTGTTCCAACTACCTTTGCTACGGCCAAGGGAT
  CCCATCACCGTGGTTACCCATCGCCGTTGGGGACGCCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004656
• ORF Size: 2190 bp
• Insert Size: 3520
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_004656.2, NP_004647.1
• RefSeq Size: 3599
• RefSeq ORF: 2190
• Locus ID: 8314
• Domains: Peptidase_C12
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]