Syntaxin 1a (STX1A) (NM_004603) Human Untagged Clone

CAT#: SC117260

STX1A (untagged)-Human syntaxin 1A (brain) (STX1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STX1A
• Synonyms: HPC-1; P35-1; STX1; SYN1A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004603 edited
  CGGGAGCATGAAGGACCGAACCCAGGAGCTCCGCACGGCCAAGGACAGCGATGATGATGA
  TGATGTCGCTGTCACCGTGGACCGAGACCGCTTCATGGATGAGTTCTTTGAGCAGGTGGA
  GGAGATTCGAGGCTTCATTGACAAGATCGCAGAGAACGTGGAGGAGGTGAAGCGGAAGCA
  CAGTGCCATCCTGGCATCCCCCAACCCCGACGAGAAGACGAAGGAGGAGCTGGAAGAACT
  CATGTCCGACATAAAGAAGACAGCAAACAAAGTTCGTTCCAAGTTAAAGAGCATCGAGCA
  GTCCATCGAGCAAGAGGAAGGCCTGAACCGCTCCTCCGCTGACCTGAGGATCCGGAAGAC
  ACAGCACTCCACGCTGTCCAGAAAGTTTGTGGAGGTCATGTCGGAGTACAACGCCACGCA
  GTCCGACTACCGCGAGCGCTGCAAAGGCCGCATCCAGAGGCAGCTGGAGATCACCGGCAG
  GACCACGACCAGTGAGGAGCTGGAGGACATGCTGGAGAGTGGGAACCCCGCCATCTTTGC
  CTCTGGGATCATCATGGACTCCAGCATCTCGAAGCAGGCTCTGAGCGAGATTGAGACGCG
  GCACAGTGAGATCATCAAGCTGGAGAACAGCATCCGTGAGCTACACGACATGTTCATGGA
  CATGGCCATGCTCGTGGAGAGCCAGGGAGAGATGATTGACAGGATCGAGTACAATGTGGA
  ACACGCGGTAGACTATGTGGAGAGGGCCGTGTCTGACACCAAGAAGGCCGTCAAGTACCA
  GAGCAAGGCGCGCCGGAAGAAAATCATGATCATCATCTGCTGTGTGATCCTGGGCATCGT
  CATCGCCTCCACTGTTGGGGGCATCTTCGCCTAGAAGCCACCCAAACTGCCACTCCACTC
  CAGGTGGGCCACTCCAAGGAGGCCCTGGCTGCTGCCACCTGGCTGGGCTGCCCTCCCAAC
  CCCCGCCTCTGGCTCAGAGCACCCTCCCTCCCGGCCCCCATGCTCCCTTCTCTGCCATGG
  GCCCTCCGTCCCCGCCCCGTGTCGTGTGCATGATCTCTGTGAGTGTGCGTCTGTACGGGA
  AGAGGCAGAGGGAGGCAGCCAGCGGGGCGTGATGCAGTGTGCACAGCGAGGAGCAGACCC
  AGGCAGGGCCGCCAGGGTGACACAGGCCACCCTTCCTTGCCTTCAGTAACTCGGTGGGCC
  CAGGTTCTGCTCTTCCCTGGGGACCCTAACCTCGCCTCCAGCTGACCTGCCCTGTCCTCT
  CCAGCTGTCCCCACAAGCAGAGCCCTGAGGGGTGGGGACCAGCTGGCCACATGGTGCTGC
  TTTTCAGGTTAGGGGAGAGGTGGCCCTGAGGGACAGCCCAGCTCTGAGTCTCAGTCGCTG
  ATCACTGCCAGGGAGGCTCAGGCTGCCATGGCTCCAGGCTCCCTCCCCTGCCTAGGGGCA
  AAGTCCATCGGGTCCTGGGCCTCAGCTTCCCTTCCCACATTCCTCCGGCCCCAGGAGCAA
  CCCCTTGGGCTAGGTCTGACCCCAGGTGTCCCTCTGGAAGGGGCTGGCTGGTGCCCTATT
  TCCAGCCACCCCAGCAGCTAGGGAGGCAAAGCAGGCTGCAGTCAGTCCCTCAAGCCAGCG
  TTGCATGTTTGGGATGGTGGCTCCTGTTGTCTTGCGCTCTGGGAAGTCAGATGTCATTTC
  AGGCCTGCAGTCTCATCCTGCCCTTGCCATCCTCCCATCGATGTGCCACGTGGGTGTCAC
  ATGTCCCAGATGCAGTATTCGGCAGCCAGCCGGGGAGGGCTACCTCCTCCTCCTCACCAC
  CTTGGGGCTTCTCATGGGAAATGTGCCCCCGCCCCAGGACCCTCTCCCTTGTGGACAGGC
  AGGGAGATGCATGCGAGTGCATGCAGCAGGGGATGGGGCCGTGTCCGTGTGCCCCACCCT
  CCCTCGGCTTTACTCCTGCCCAGTGACTGTGACCACTGTCCGTGTTGCCTTCTTGAACAG
  CGATTCCCCCCAACCCCTTCACCAAAGGTCTTGGTACAACCAGCTGCCCATTTTGTGAAA
  TTTTTATGTAGAATAAACATTTGTATCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004603
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_004603.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004603.1, NP_004594.1
• RefSeq Size: 2088 bp
• RefSeq ORF: 867 bp
• Locus ID: 6804
• Cytogenetics: 7q11.23
• Domains: t_SNARE, SynN
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).