Transglutaminase 2 (TGM2) (NM_004613) Human Untagged Clone

CAT#: SC117263

TGM2 (untagged)-Human transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) (TGM2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGM2
• Synonyms: TG(C); TGC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117263 sequence for NM_004613 edited (data generated by NextGen Sequencing)
  ATGGCCGAGGAGCTGGTCTTAGAGAGGTGTGATCTGGAGCTGGAGACCAATGGCCGAGAC
  CACCACACGGCCGACCTGTGCCGGGAGAAGCTGGTGGTGCGACGGGGCCAGCCCTTCTGG
  CTGACCCTGCACTTTGAGGGCCGCAACTACGAGGCCAGTGTAGACAGTCTCACCTTCAGT
  GTCGTGACCGGCCCAGCCCCTAGCCAGGAGGCCGGGACCAAGGCCCGTTTTCCACTAAGA
  GATGCTGTGGAGGAGGGTGACTGGACAGCCACCGTGGTGGACCAGCAAGACTGCACCCTC
  TCGCTGCAGCTCACCACCCCGGCCAACGCCCCCATCGGCCTGTATCGCCTCAGCCTGGAG
  GCCTCCACTGGCTACCAGGGATCCAGCTTTGTGCTGGGCCACTTCATTTTGCTCTTCAAC
  GCCTGGTGCCCAGCGGATGCTGTGTACCTGGACTCGGAAGAGGAGCGGCAGGAGTATGTC
  CTCACCCAGCAGGGCTTTATCTACCAGGGCTCGGCCAAGTTCATCAAGAACATACCTTGG
  AATTTTGGGCAGTTTGAAGATGGGATCCTAGACATCTGCCTGATCCTTCTAGATGTCAAC
  CCCAAGTTCCTGAAGAACGCCGGCCGTGACTGCTCCCGCCGCAGCAGCCCCGTCTACGTG
  GGCCGGGTGGTGAGTGGCATGGTCAACTGCAACGATGACCAGGGTGTGCTGCTGGGACGC
  TGGGACAACAACTACGGGGACGGCGTCAGCCCCATGTCCTGGATCGGCAGCGTGGACATC
  CTGCGGCGCTGGAAGAACCACGGCTGCCAGCGCGTCAAGTATGGCCAGTGCTGGGTCTTC
  GCCGCCGTGGCCTGCACAGTGCTGAGGTGCCTGGGCATCCCTACCCGCGTCGTGACCAAC
  TACAACTCGGCCCATGACCAGAACAGCAACCTTCTCATCGAGTACTTCCGCAATGAGTTT
  GGGGAGATCCAGGGTGACAAGAGCGAGATGATCTGGAACTTCCACTGCTGGGTGGAGTCG
  TGGATGACCAGGCCGGACCTGCAGCCGGGGTACGAGGGCTGGCAGGCCCTGGACCCAACG
  CCCCAGGAGAAGAGCGAAGGGACGTACTGCTGTGGCCCAGTTCCAGTTCGTGCCATCAAG
  GAGGGCGACCTGAGCACCAAGTACGATGCGCCCTTTGTCTTTGCGGAGGTCAATGCCGAC
  GTGGTAGACTGGATCCAGCAGGACGATGGGTCTGTGCACAAATCCATCAACCGTTCCCTG
  ATCGTTGGGCTGAAGATCAGCACTAAGAGCGTGGGCCGAGACGAGCGGGAGGATATCACC
  CACACCTACAAATACCCAGAGGGGTCCTCAGAGGAGAGGGAGGCCTTCACAAGGGCGAAC
  CACCTGAACAAACTGGCCGAGAAGGAGGAGACAGGGATGGCCATGCGGATCCGTGTGGGC
  CAGAGCATGAACATGGGCAGTGACTTTGACGTCTTTGCCCACATCACCAACAACACCGCT
  GAGGAGTACGTCTGCCGCCTCCTGCTCTGTGCCCGCACCGTCAGCTACAATGGGATCTTG
  GGGCCCGAGTGTGGCACCAAGTACCTGCTCAACCTCAACCTGGAGCCTTTCTCTGAGAAG
  AGCGTTCCTCTTTGCATCCTCTATGAGAAATACCGTGACTGCCTTACGGAGTCCAACCTC
  ATCAAGGTGCGGGCCCTCCTCGTGGAGCCAGTTATCAACAGCTACCTGCTGGCTGAGAGG
  GACCTCTACCTGGAGAATCCAGAAATCAAGATCCGGATCCTTGGGGAGCCCAAGCAGAAA
  CGCAAGCTGGTGGCTGAGGTGTCCCTGCAGAACCCGCTCCCTGTGGCCCTGGAAGGCTGC
  ACCTTCACTGTGGAGGGGGCCGGCCTGACTGAGGAGCAGAAGACGGTGGAGATCCCAGAC
  CCCGTGGAGGCAGGGGAGGAAGTTAAGGTGAGAATGGACCTGCTGCCGCTCCACATGGGC
  CTCCACAAGCTGGTGGTGAACTTCGAGAGCGACAAGCTGAAGGCTGTGAAGGGCTTCCGG
  AATGTCATCATTGGCCCCGCCTAA
  
  Clone variation with respect to NM_004613.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004613 unedited
  NCCGTTCAGATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACCAGGCCA
  GTGGTCGCACTTGGAGGGTCTCGCCGCCAGTGGAAGGAGCCACCGCCCCCGCCCGACATG
  GCCGAGGAGCTGGTCTTAGAGAGGTGTGATCTGGAGCTGGAGACCAATGGCCGAGACCAC
  CACACGGCCGACCTGTGCCGGGAGAAGCTGGTGGTGCGACGGGGCCAGCCCTTCTGGCTG
  ACCCTGCACTTTGAGGGCCGCAACTACGAGGCCAGTGTAGACAGTCTCACCTTCAGTGTC
  GTGACCGGCCCAGCCCCTAGCCAGGAGGCCGGGACCAAGGCCCGTTTTCCACTAAGAGAT
  GCTGTGGAGGAGGGTGACTGGACAGCCACCGTGGTGGACCAGCAAGACTGCACCCTCTCG
  CTGCAGCTCACCACCCCGGCCAACGCCCCCATCGGCCTGTATCGCCTCAGCCTGGAGGCC
  TCCACTGGCTACCAGGGATCCAGCTTTGTGCTGGGCCACTTCATTTTGCTCTTCAACGCC
  TGGTGCCCAGCGGATGCTGTGTACCTGGACTCGGAAGAGGAGCGGCAGGAGTATGTCCTC
  ACCCAGCAGGGCTTTATCTACCAGGGCTCGGCCAAGTTCATCAAGAACATACCTTGGAAT
  TNTGGGCAGTTTGAAGATGGGATCCTAGACATCTGCCTGATCCTTCTANGATGTCAACCC
  CAGTTCCTGAAGAACGCCGGCCGTGACTGCTCCCGCCGCAGCAGCCCCGTCTACGTGGGC
  CGGGTGGTGAGTGGCATGGTCAACTGNCACGATGACCANGGTGTGCTGCTGGGACGCTGG
  GACAACACTACGGGGACGGCGTCAGCCCATGTNCTGGATCGGCAGCGTGGACATCCTGCN
  GCGCTGGAAGAACACNGCTGCCANCGCGTCAAGTATGGCCAGTGCTGGGNTCTCNNNCGN
  CGTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004613 unedited
  CCATAAACACCTTCTTTTNNGGAGAGGGAGCCATCAGAGTCGAGTTTTTTTTTTTTTTTC
  TTTTATTTCCGAGTGTGAAAAATAAACTCCCTTTAAATCAGNGNNANGNACCATTGCGGG
  TCAAAAGCACATCCCTTTGAAAGGNGTGGAACCCAAGGCTTTATATAAAACCAGTAAAGA
  AATAGAGCTCTATGTATAGAGAAAATATACATGTCTGAAAAATGGGTGACTCTTTCCTGA
  GCAAAGAAGAAAGATCAAAATGCAACAGAATGATTCTCTCCAAGACCATCCCTGGGAATT
  GGGAGGCCCTGGAGGCTGTGATCTCTACCTAAAACAGAAAATCCCCAAATCTTTCAGCCT
  AAGGGAAGCACTGTCAAGTAAAAAGAGCAGGAGATAAAGTTCAAAGCATGACAACTCATG
  GGATCCACAAGCTACCTCCGGAGCAGGGGCTATGTTTGAAAAGCCTAATACTTGATAGGA
  ATGGAAAGATGGAAGAGTTAATCTTGTAGGATAAGCGACCAGCACTGATAATATAAAGAA
  AGATAGGAATAACTTGTCATTAGCAACGAGAAGGATCAAGAGAAGAGAAATGGAAGACAA
  CGATCAACACACCTATAATTGGAATCCGAAGGAAGAGAACTGAAACAAAAAAAGGAACCG
  ATTAAAATATTATTAACAAAAAGTGGGAAGCACACAAGTTTAACATCTAATAAAACTATT
  AAATAAAAAATCGAATAATGTCAGATCAAAAAGTGAAAATTAAATCTATGCACATTTCAA
  GTGTAAGGTCAAACACAGAACAATACATTACAACTTAATGGAAAAGAATCCTCAAAAAAA
  GACGAAAACTAACATGCCTATAGTATGAAAAACGAATAAAAATATAAACAATCTCAAAAA
  CTAAAAAGCTATTAATATTAGTAAAAAAATAAAAAATATATAATCAATAAAAGATCTATA
  TGAAAAAAATAGATAACTAGAGAAAAACATATATGAATAAAAATTGATATACAGTAAAAA
  ATAAATATTACATGAATGATTGTACTCATAATCAGAATCTCAAAACTAGAATAAAGAAAA
  AGTGTATAGAATATTCAATAGAATCAAAATATTACCTAAATATATCACCAAGAATCTTAT
  ATAATAAGAACACTGAAAATATTTATAAATTATAAGAGATGAGCAGCCTATAAAAACGTG
  CTCCATTACTCCTTGGTATGAATAACACATATATAGATAATTCTAATAAGATATGCAGCA
  TAAGCATATCTCGGAAACGACGGAGTGATAATTCATAAAATCACTATATGATTATTAAGA
  ACTACTGATATATCATAAATAAGGATTCGACTACATGATTATATTGGTATCGTAATCGAT
  GAGTCAATATGACAACTAATATACGATACATGGAGTCATATTCATATACTGACTTATCAC
  ACTTACACAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004613
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004613.2, NP_004604.2
• RefSeq Size: 3937 bp
• RefSeq ORF: 2064 bp
• Locus ID: 7052
• Cytogenetics: 20q11.23
• Domains: Transglutamin_C, TGc
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• Gene Summary: 'Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.